Emad Hashish is a scholar visitor at Kansas State University who received his PhD from South Dakota State University. He has worked in research and teaching roles in Egypt, Germany, and Canada. His research focuses on developing vaccines for diseases like E. coli and BVDV. He has published several papers in this area and received funding for his work. Dr. Hashish utilizes techniques like PCR, protein analysis, and bioinformatics in his research.

1. 1
Emad Hashish, Ph.D.
Scholar Visitor, Department of Diagnostic Medicine Pathobiology, College of veterinary
medicine, K-State University, USA. emadhashish@vet.k-state.edu
Education & career employment
• January 2015- present: Scholar Visitor, Department of Diagnostic Medicine/Pathobiology
(DMP), College of veterinary medicine, KSU, USA.
• July 2014- January 2015: Scholar Visitor, Friedrich-Loeffler-Institute (FLI) for Animal
Health, Jena, Germany.
• June 2013- June 2014: Assistant professor, Clinical Pathology Department, Faculty of
Veterinary Medicine, Zagazig University, Egypt.
• August 2010- May 2013: Research assistant, South Dakota State University, Brookings, SD,
USA.
• September 2008 -July 2010: Teaching assistant, Clinical Pathology Department, Faculty of
Veterinary Medicine, Zagazig University, Egypt.
• March 2008- August 2008: Scholar Visitor, Ontario Veterinary College (OVC), Guelph
University, Canada.
• September 2004-February 2008: Teaching assistant, Clinical Pathology and Laboratory
Diagnosis Department, Faculty of Veterinary Medicine, Zagazig University, Egypt.
• 1998-2003: Undergraduate, Faculty of Veterinary Medicine, Zagazig University, Egypt.
• Publications
− Hashish EA, El Damaty HM, Tartor YH and Gouda SM (2015): Multiplex PCR for The
Identification of Diarrheagenic Escherichia coli in Newborn Calves in Egypt: Virulence
Genes Characterization and Clincobiochemical Assays. Under publication.
− Dlabola J, Hashish EA, Pauly B, Kubisiak B, Behm I, Heseler R, Schilephake A, Wieler L,
Neubauer H, Seyboldt C (2015): Clostridium botulinum type D/C intoxication in a dairy cow
stock in Saxony-Anhalt (Germany). Under publication.
− Hashish EA, Elgaml SA, El-Murr A, Khalil R (2015): Nephroprotective and antioxidant
significance of selenium and α-tocopherol on lead acetate-induced toxicity of Nile Tilapia
(Oreochromis niloticus). Fish Physiology and Biochemistry.

2. 2
− Amro AA, Neama AJ, Hussein A, Hashish EA, Sheweita SA (2014): Evaluation the Surface
Antigen of the Salmonella typhimurium ATCC 14028 Ghosts Prepared by "SLRP". Sci
World J. 2014: 1-6.
− Hashish EA, Zhang C, Ruan X, Knudsen DE, Chase CC, Isaacson RI, Zhou G, Zhang W
(2013): A multi-epitope fusion antigen elicited neutralizing antibodies against
enterotoxigenic E. coli (ETEC) and bovine viral diarrhea virus (BVDV) in vitro. ClinVaccine
Immunol. 20 (7): 1076-83.
− Hashish EA (2013): Development of a Subunit Vaccine Against Bovine Diarrhea Caused by
K99 Enterotoxigenic Escherichia Coli and Bovine Viral Diarrhea Virus. Dissertation, South
Dakota State University.
Research experience
1- PCR, Western blotting & tissue culture.
2- DNA, RNA extraction & cloning.
3- Protein extraction & purification.
4- ELISA & immunohistochemistry.
5- Bio-informatics software for B & T cell epitopes prediction.
6- MALDI-TOF MS for bacterial protein identification.
7- Clinicobiochemical & hematological diagnostic assays.
• Memberships, awards & fellowships:
• 2015, awarded a short-term fellowship from the STDF-STF, project number 6692, role
principal investigator (PI), project entitled: Vaccine development against selective virulence
factors from the enterotoxigenic E. Coli. To be carried out at the DMP, KS, USA.
• 2014, awarded a short-term postdoctoral research scholarship, from DAAD, host is Friedrich-
Loeffler-Institute (FLI) for Animal Health, Jena, Germany.
• 2012, awarded honored membership of the Honor Society of Phi Kappa Phi for the scientific
excellence and top academic achievement at the South Dakota State University, USA.
• 2010, research assistantship, Ph.D. program, South Dakota State University (SDSU), USA.
• 2008, awarded a short-term scholarship form the ministry of higher education (MHESR),
Egypt for research training in the Pathobiology Department, OVC, Guelph University,
Canada.
• 2003, honor certificate of General Egyptian Veterinary syndicate for the graduate scientific

3. 3
excellence.
• 2001, undergraduate scientific award of excellence, faculty of Veterinary Medicine, Zagazig
University.
Scientific Symposia and Conferences
• Internal FluResearchNet Meeting, 4th International Influenza Meeting (2014), University of
Muenster, Germany.
• Conference of research workers in animal diseases, (CRWAD), Chicago, IL, USA, oral
presentation entitled: Hashish E et al., (2012) A novel vaccine candidate protecting cattle
against diarrhea caused by enterotoxigenic Escherichia coli (ETEC) and bovine viral diarrhea
virus (BVDV).
• 72nd
Annual Meeting, North Central Branch of the American Society for Microbiology
(ASM) October 12th
-13th
, 2012 North Dakota State University, Fargo, ND, USA. Hashish E
et al., (2012) multivalent vaccine protecting against diarrhea caused by enterotoxigenic
Escherichia coli (ETEC) and bovine viral diarrhea virus (BVDV).
• John H and Amy Bowles Lawrence Foundation Symposium attendance, Global Frontiers in
Vaccine Development. Sanford School of Medicine, October 6th
, 2011, Sioux Falls, SD,
USA & October 7th
, 2011 in Vermillion, SD, USA.
Training Courses & certification
• Odyssey Infrared imaging system Training (Licor), South Dakota State University, 2011.
• Institutional Animal Care and Use (IACUC), online training for mice, rat and pig, 2010,
2015.
• Research ethics, Collaborative Institutional Training Initiative (CITI) online training, 2010.
• Practical laboratory course, Alborg diagnostic laboratory, 2009.
• International Computer Driving License (ICDL) certification, 2009.
• Clinical Pathology and diagnostic laboratory courses of Skills Training & Enhancement
Program, "VSTEP” at Ontario Veterinary College, Guelph, Ontario, Canada, 2008.
• Advanced Courses and laboratory training on Clinical Pathology at Ontario Veterinary
College (OVC), Guelph, Ontario, Canada, 2008.
• Bundle of soft skills development courses, SSEG, Zagazig University as part of Faculty and
leadership development program includes, Effective Teaching Training course (20 hours),

4. 4
Thinking Skills for (15 hours), Communication Skills (15 hours), Decision and Problem
Solving (15 hours), Ethics of Teaching (15 hours), Basis of Scientific Research (20 hours)
• Training course on computer science, SPSS, MS office, Internet and Windows, 2006.
References
• Dr. Christian Seyboldt
Friedrich-Loeffler-Institute for Animal Health
Naumburger Str. 96a | 07743 Jena, Germany.
Tel: +49 (364) 180-42295
Email: Christian.Seyboldt@fli.bund.de
• Dr. Heinrich Neubauer
Head of Friedrich-Loeffler-Institute for Animal Health
Naumburger Str. 96a | 07743 Jena, Germany.
Tel: +49 (364) 180-42200
Email: Heinrich.Neubauer@fli.bund.de
• Dr. Ying Fang
Department of diagnostic medicine/pathobiology,
Kansas State University, Manhattan, Kansas, USA.
Phone: +1 (785) 532-4452
E-mail: yfang@vet.k-state.edu
• Dr. Wafaa Eleraky
Dean, Faculty of Veterinary Medicine
Zagazig University, Zagazig, Egypt.
Phone: +2 (010) 905-20921
E-mail: wafaaeleraky@yahoo.com
• Dr. Darren Wood
Department of Pathobiology,
University of Guelph, Guelph, Ontario, Canada.
Phone: +1(519)-824-4120 ex. 54654
E-mail: woodr@uoguelph.ca