The Role of Sylepta Derogata[Lepidoptera: Pyralidae] In The Abscission And De...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
4 ijfaf nov-2017-2-allelopathic effect of eucalyptusAI Publications
Laboratory and greenhouse experiments were carried out at the Faculty of Agricultural Sciences, University of Gezira, Sudan in season 2014/15 to study the allelopathic effects of Eucalyptus (Eucalyptus camaldulensis Dehnh) leaf on seed germination and seedling growth of some poaceous crops. Laboratory experiments were conducted to study the allelopathic effects of leaf aqueous extract of Eucalyptus on seed germination of sorghum (Sorghum bicolor [L.] Moench), millet (Pennisetum glaucum [L.] R. Br.), maize (Zea mays L.) and wheat (Triticum vulgare L.). Six concentrations (0, 20, 40, 60, 80 and 100%) of the leaf aqueous extract of Eucalyptus were prepared from the stock solution (50 g / l). Treatments, for each crop, were arranged in completely randomized design with four replicates. The seeds were examined for germination at three days after initial germination. Greenhouse experiments were conducted to study the allelopathic effects of Eucalyptus leaf powder on seedling growth of the same crops. The leaf powder of Eucalyptus was incorporated into the soil at rate of 0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0% on w/w bases in pots. Treatments, for each crop, were arranged in completely randomized design with four replicates. The experiments were terminated at 30 days after sowing and the plant height, number of leaves and root length of crop seedlings were measured as well as plant fresh and dry weight. Data were subjected to analysis of variance procedure. Means were separated for significance using Duncan`s Multiple Range Test at p 0.5. The results showed that the leaf aqueous extract of Eucalyptus significantly reduced seed germination of the tested poaceous crops and there was direct negative relationship between concentration and germination. Also, the results showed that incorporating leaf powder of Eucalyptus into the soil significantly decreased plant height, number of leaves and root length of crop seedlings as well as seedling fresh and dry weight. In addition, the reduction in seedling growth was increased as the leaf powder increased in the soil. Based on results supported by different studies, it was concluded that Eucalyptus has allelopathic effects on seed germination and seedling growth of the poaceous crops.
Controlled environment system and method for rapid propagation of saba banana...Innspub Net
Conventional propagation practices of banana challenge the production of disease-free planting materials. This study evaluates the use of misting system and different plant growth enhancers, Benzyl Amino Purine at 2mg/l and Napthalene Acetic Acid at 0.93g/L, on plantlet development of Saba banana (Musa balbisiana) macropropagated under glasshouse conditions. A total of 36 corms are equally distributed in three propagators. Four growth parameters are observed and analysed using factorial in Completely Randomized Design in first generation plantlets (GP1) and second generation plantlets (GP2). Results show that the use of misting system significantly increased (p<0.01) all the growth parameters tested during the first and second cycles. The growth enhancers significantly shortened the number of days to emergence (p<0.01), (GP1, GP2) and increased the number of shoots emerged (p<0.01) (GP1, GP2), shoot collar diameter (p<0.01) (GP1) (p<0.05) (GP2), and total leaf area (p<0.05) (GP1) (p<0.01) (GP2). The interaction of the two factors has significantly shortened the number of days to emergence (P ≤ 0.05), produced the most number of shoots (P ≤ 0.01) and the largest total leaf area (P ≤ 0.05) in GP2. The findings suggest that the combined use of misting system and plant growth enhancers accelerates the growth of macropropagated Saba banana.
Evaluating Kenyan Dolichos (Lablab purpureus L.) Genotypes for Resistance to ...Premier Publishers
The objective of this study was to evaluate eighteen Dolichos lablab genotypes for resistance to Maruca vitrata and Helicoverpa armigera in the field using morphological markers. The study design was Randomized Complete Block (RCBD) with separation of mean done using Turkey’s range of test. Eldoret, KALRO Njoro and KALRO Kakamega were the study sites. Morphological parameters of pods were studied to determine whether they have any influence on resistance of Dolichos lablab to M. vitrata and H. armigera. The pod damage (%) of each genotype was calculated and given a resistance rating of 1-5 score damage. Genotype G2, Bahati and W7 were resistant to M. vitrata in a scale of 1(0-10%; low infestation), Bahati and W7 were moderately resistant to H. armigera in a scale of 2(11-30%; moderate infestation). Genotype LG1MoiP10 was susceptible to M. vitrata in a scale of 4 (51-70%; severe infestation) and genotype M5 was intermediate to H. armigera in a scale of 3 (31-50%; high infestation). There was positive significant correlation in H. armigera and M. vitrata pod damage with days to maturity, growth habit, and pod attachment. Pod length and pod fragrance were positively correlated to M. vitrata. Negative correlation was detected in pod thickness, pod pubescence and raceme position to pod damage by H. armigera and M. vitrata. The study identified G2, Bahati and W7 as promising resistant genotypes and can be used in Dolichos breeding program. However, there is need to further evaluate them in different environments and seasons for reliability.
In the laboratory of plant physiology of the University of Queensland (Gatton Campus), a seed germination experiment was undertaken on seeds of two Australian native plant species – Rhodanthe floribundato and Acacia aneura. Most Acacia, including A. aneura exhibit a physical dormancy due to the waxy coat covering the seed. Comparably, just a few species of Rhodanthe are studied as to their dormancy. However, they are also known to present different forms of dormancy. To understand and describe these dormancy mechanisms, a seed germination experiment was conducted on Acacia aneura and Rhodanthe floribunda. This experiment will either add to the existing knowledge regarding these species’ dormancy or corroborate them. It is expected that both species display some form(s) of dormancy.
ABSTRACT- Phylloplane fungi presented on the leaf surface were screened and selected for the assessment of their
potential against Alternaria alternata causing leaf spot disease of Rauwolfia serpentina (Sarapgandha). Among the
antagonists Trichoderma harzianum ISO-2 showed minimum conidial germination. Effect of foliar spray of phylloplane
fungi on the lesion development was also studied. In mist chamber studies seven treatments comprising application of
antagonists in the presence of pathogen proved effective in causing per cent disease reduction by 30-50 % in comparison
with the control. In the field trials Penicillium sublateritium showed the maximum fresh and dry root weight. P. sublateritium
also showed significant percentage disease reduction in comparison to control.
Key words- Alternaria alternata, Foliar spray, Leaf spot, Phylloplane fungi, Rauwolfia serpentina
The Role of Sylepta Derogata[Lepidoptera: Pyralidae] In The Abscission And De...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
4 ijfaf nov-2017-2-allelopathic effect of eucalyptusAI Publications
Laboratory and greenhouse experiments were carried out at the Faculty of Agricultural Sciences, University of Gezira, Sudan in season 2014/15 to study the allelopathic effects of Eucalyptus (Eucalyptus camaldulensis Dehnh) leaf on seed germination and seedling growth of some poaceous crops. Laboratory experiments were conducted to study the allelopathic effects of leaf aqueous extract of Eucalyptus on seed germination of sorghum (Sorghum bicolor [L.] Moench), millet (Pennisetum glaucum [L.] R. Br.), maize (Zea mays L.) and wheat (Triticum vulgare L.). Six concentrations (0, 20, 40, 60, 80 and 100%) of the leaf aqueous extract of Eucalyptus were prepared from the stock solution (50 g / l). Treatments, for each crop, were arranged in completely randomized design with four replicates. The seeds were examined for germination at three days after initial germination. Greenhouse experiments were conducted to study the allelopathic effects of Eucalyptus leaf powder on seedling growth of the same crops. The leaf powder of Eucalyptus was incorporated into the soil at rate of 0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0% on w/w bases in pots. Treatments, for each crop, were arranged in completely randomized design with four replicates. The experiments were terminated at 30 days after sowing and the plant height, number of leaves and root length of crop seedlings were measured as well as plant fresh and dry weight. Data were subjected to analysis of variance procedure. Means were separated for significance using Duncan`s Multiple Range Test at p 0.5. The results showed that the leaf aqueous extract of Eucalyptus significantly reduced seed germination of the tested poaceous crops and there was direct negative relationship between concentration and germination. Also, the results showed that incorporating leaf powder of Eucalyptus into the soil significantly decreased plant height, number of leaves and root length of crop seedlings as well as seedling fresh and dry weight. In addition, the reduction in seedling growth was increased as the leaf powder increased in the soil. Based on results supported by different studies, it was concluded that Eucalyptus has allelopathic effects on seed germination and seedling growth of the poaceous crops.
Controlled environment system and method for rapid propagation of saba banana...Innspub Net
Conventional propagation practices of banana challenge the production of disease-free planting materials. This study evaluates the use of misting system and different plant growth enhancers, Benzyl Amino Purine at 2mg/l and Napthalene Acetic Acid at 0.93g/L, on plantlet development of Saba banana (Musa balbisiana) macropropagated under glasshouse conditions. A total of 36 corms are equally distributed in three propagators. Four growth parameters are observed and analysed using factorial in Completely Randomized Design in first generation plantlets (GP1) and second generation plantlets (GP2). Results show that the use of misting system significantly increased (p<0.01) all the growth parameters tested during the first and second cycles. The growth enhancers significantly shortened the number of days to emergence (p<0.01), (GP1, GP2) and increased the number of shoots emerged (p<0.01) (GP1, GP2), shoot collar diameter (p<0.01) (GP1) (p<0.05) (GP2), and total leaf area (p<0.05) (GP1) (p<0.01) (GP2). The interaction of the two factors has significantly shortened the number of days to emergence (P ≤ 0.05), produced the most number of shoots (P ≤ 0.01) and the largest total leaf area (P ≤ 0.05) in GP2. The findings suggest that the combined use of misting system and plant growth enhancers accelerates the growth of macropropagated Saba banana.
Evaluating Kenyan Dolichos (Lablab purpureus L.) Genotypes for Resistance to ...Premier Publishers
The objective of this study was to evaluate eighteen Dolichos lablab genotypes for resistance to Maruca vitrata and Helicoverpa armigera in the field using morphological markers. The study design was Randomized Complete Block (RCBD) with separation of mean done using Turkey’s range of test. Eldoret, KALRO Njoro and KALRO Kakamega were the study sites. Morphological parameters of pods were studied to determine whether they have any influence on resistance of Dolichos lablab to M. vitrata and H. armigera. The pod damage (%) of each genotype was calculated and given a resistance rating of 1-5 score damage. Genotype G2, Bahati and W7 were resistant to M. vitrata in a scale of 1(0-10%; low infestation), Bahati and W7 were moderately resistant to H. armigera in a scale of 2(11-30%; moderate infestation). Genotype LG1MoiP10 was susceptible to M. vitrata in a scale of 4 (51-70%; severe infestation) and genotype M5 was intermediate to H. armigera in a scale of 3 (31-50%; high infestation). There was positive significant correlation in H. armigera and M. vitrata pod damage with days to maturity, growth habit, and pod attachment. Pod length and pod fragrance were positively correlated to M. vitrata. Negative correlation was detected in pod thickness, pod pubescence and raceme position to pod damage by H. armigera and M. vitrata. The study identified G2, Bahati and W7 as promising resistant genotypes and can be used in Dolichos breeding program. However, there is need to further evaluate them in different environments and seasons for reliability.
In the laboratory of plant physiology of the University of Queensland (Gatton Campus), a seed germination experiment was undertaken on seeds of two Australian native plant species – Rhodanthe floribundato and Acacia aneura. Most Acacia, including A. aneura exhibit a physical dormancy due to the waxy coat covering the seed. Comparably, just a few species of Rhodanthe are studied as to their dormancy. However, they are also known to present different forms of dormancy. To understand and describe these dormancy mechanisms, a seed germination experiment was conducted on Acacia aneura and Rhodanthe floribunda. This experiment will either add to the existing knowledge regarding these species’ dormancy or corroborate them. It is expected that both species display some form(s) of dormancy.
ABSTRACT- Phylloplane fungi presented on the leaf surface were screened and selected for the assessment of their
potential against Alternaria alternata causing leaf spot disease of Rauwolfia serpentina (Sarapgandha). Among the
antagonists Trichoderma harzianum ISO-2 showed minimum conidial germination. Effect of foliar spray of phylloplane
fungi on the lesion development was also studied. In mist chamber studies seven treatments comprising application of
antagonists in the presence of pathogen proved effective in causing per cent disease reduction by 30-50 % in comparison
with the control. In the field trials Penicillium sublateritium showed the maximum fresh and dry root weight. P. sublateritium
also showed significant percentage disease reduction in comparison to control.
Key words- Alternaria alternata, Foliar spray, Leaf spot, Phylloplane fungi, Rauwolfia serpentina
Dormancy breaking and the influence of gibberellic acid on the early growth o...Innspub Net
The purpose of this study was to determine the appropriate methods of breaking seed dormancy, level of water uptake, and the influence of gibberellic acid on the early growth of Tamarindus indica seedlings. The scarification methods used included: concentrated sulphuric acid (H2SO4), manual scarification, flaming, hot and cold water treatments. The experiments were conducted in the laboratory on Petri dishes and in potting media. GA3 was used to optimize the production of seedlings by spraying the solution on the foliage. The treatment for 10 minutes with Conc. H2SO4 gave the maximum germination percentage and water uptake (80.41%). GA3 enhances the growth of the seedlings by increasing the height, the number of leaves and stem girth at eight weeks after sowing. In conclusion, all the scarification treatments applied to the seeds of T. indica proved effective. The ten (10) minutes treatment with concentrated H2SO4 was the best treatment for breaking seed dormancy of T. indica. And gibberellic acid enhanced early and fast seedling growth as it increase height, number of leaves and stem girth of T. indica.
This study was carried out on the mycoflora associated with seeds of different citrus species. Citrus seed material was collected from districts of Punjab, i.e. Multan, Sargodha and Khanpur. Standard methods were applied for the isolation and identification of fungi. A total of 11 fungi including Aspergillus fumigatus, Aspergillus flavus, Dreschslera tetramera, Alternaria alternata, Curvularia lunata, Macrophomina phaseolina, Aspergillus niger, Fusarium solani, Fusarium moniliforme, Rhizopus and Penicillium spp were isolated from the seeds of citrus. For control of isolated seed-born fungi, 3 recommended fungicides such as Ridomil Gold, Bavistin, Score and two chemical Salicylic acid and Boric acid, were used at 20, 30, 40 mg/10 mL and 5, 6, 7 μL/10 mL, respectively and chemical with 20, 30, 40 mg/10 mL. All these fungicide and chemicals significantly reuced with population of all fungi present in naturally infected seed samples. Ridomil Gold and Salicylic acid were found to be the best for the control of se d-born fungi of citrus seed at 40 mg/10 mL. The isolation and identification of different mycotoxins is essential to study health status of the citrus consumers and to safeguard the standards of WTO.
Turbidity and Colour Reduction of Pond Water Using Extracts of Diospyros mesp...AZOJETE UNIMAID
The study examined the coagulation performance of leaves and root bark extracts of Diospyros mespiliformis, Mitragyna inermis, Piliostigma reticulatum and Tamarindus indica. The plant materials were collected along River Kubwa in Damboa local Government area of Borno State, Nigeria. The crude extracts of these plant materials were prepared based on soxhlet method using soxhlet solvent extractor. About 400mg of each pulverized sample was used for the extraction process. Three (3) liters of distilled water was used for the extraction process. The extracts were each added to highly turbid (422 NTU) pond water samples at various dosages of 60, 75, 90, 105, 120, and 135mg/l and a conventional coagulation-flocculation jar tests were conducted. The nature of the flocs formation for all the plant extracts were observed to be smoky rather than aggregating into denser masses and resulted in turbidity and colour removal efficiencies of 0.71-18.57% and 1.17-22.38% respectively. The coagulation activities were subjected to T-Test Analysis and it indicated that there was statistically (P< 0.05) significance difference at different concentrations. The results also revealed slight decrease in pH in the range of 8.1-7.55 exhibited by all the extracts. From the results of the turbidity and colour removal efficiencies, it can be concluded that these plant materials have the potential of improving the quality of pond or surface water (which most rural communities in developing countries depend for many purposes) that are heavily turbid; However, for them to perform effectively, a coagulant aid is required.
Evaluation of the Activity of Insecticides Plants in the Far North Region of ...IJEAB
This study proposes to assess the activity of insecticide plants in the far North region of Cameroon. The leaves or bark of four local plants (Azadirachtaindica, Boswelliadalzeilii, Khayasenegalensis and Ocimumcanum) were harvested, dried and powdered for the formulation of insecticidal chopsticks at different doses. Toxicity tests have been conducted on adult culicidae mosquitoes by fumigation. They reveal low levels of mortality after 15 minutes of exposure to the smoke of the chopsticks. Remanence due to chopsticks smoke leads to high rates of mortality after 6 and 24 hours of exposure. Mortality rates increase with the dose of each vegetable powder. Lethal doses were calculated 6 hours after exposure for each plant powder. Those of the leaves of Azadirachtaindica proved to be the most efficient thus with the lowest LD50 value of 36.14%. These vegetable powders can be used as natural insecticides instead of chemical insecticides.
Pharmacological activity of the methanolic extract of sea urchins against esc...Innspub Net
This study elucidated the pharmacological potential of sea urchins using methanol as extracting medium. The antibacterial potential was evaluated using the paper disc method and zone of inhibition against Escherichia coli and Staphylococcus aureus was measured. Antioxidant properties of sea urchins were evaluated using DPPH radical scavenging assay. Three species of sea urchin randomly collected along the intertidal zone of Diguisit, Baler Aurora were identified using diagnostic keys by the National Museum of the Philippines and they were identified as follows; Echinothrix diadema, Echinometra mathaei, and Echinometra oblonga. E. diadema recorded the highest diameter zone of inhibition against E. coli and S. aureus after 24 hours of incubation with 11.03 ± 1.75mm and 13.52 ± 1.13mm respectively while E. mathaei only inhibited S. aureus with zone of inhibition of 9.27 ± 2.06mm in 24 hours of incubation as well. As the zone of inhibition prolongs, the zone of inhibition decreases as observed in 48 hours of incubation. E. oblonga did not show inhibitoy effect, however it recorded the highest radical scavenging activity with 64.46% among the three species of sea urchins. This was followed by E. mathaei (51.52%) and E. diadema (37.38%). All collected species manifested antioxidant potential. Based on the results, the collected species of sea urchins has a pharmacological potential.
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
The papers for publication in The International Journal of Engineering& Science are selected through rigorous peer reviews to ensure originality, timeliness, relevance, and readability.
Efficacy of aqueous extracts of Aloe zebrina Baker, Capsicum annum L. and Mel...Innspub Net
Low cotton yields in Zimbabwe are mainly caused by infestation by high numbers of insect pests, diseases, poor agronomic practices and poor season quality. Aphids cause considerable yield loss in cotton. Control methods implemented for aphids include cultural approaches, chemical and biological control and use of biopesticides. Synthetic pesticides used by farmers have problems of environmental contamination, toxicity to non-target organisms, resistance by pests and toxicity to humans among others. Plant extracts (PEs) are suitable alternatives to synthetic pesticides. Three aqueous PEs at 10 and 20%v/v application rates, distilled water (no spray) and acetamiprid were evaluated against aphids. The 3×2 factorial + 2 experiments were laid out in an RCBD in the field and CRD for laboratory experiments. Leaves of Aloe zebrina and Melia azedarach and Capsicum annum fruits were dried and ground into powder. Water extracts were prepared and used at 10 and 20%v/v and compared with synthetic insecticide and distilled water in the laboratory. In field evaluations distilled water was replaced by unsprayed plots. All three PEs had insecticidal properties against aphids. During laboratory experiments C. annum 20%v/v killed 64.75% aphids and C. annum 10%v/v killed 63.85% aphids and they were significantly (p=0.007) different from other treatments after correcting for mortality. An average of 80.3% and 72.2% aphids were controlled by C. annum 20%v/v and A. zebrina 20%v/v during field trial, these were significant (p<0.001). A. zebrina extracts had both repellence and contact toxicity against aphids. All the PEs are suitable alternatives to aphicides for aphid management.
Dormancy breaking and the influence of gibberellic acid on the early growth o...Innspub Net
The purpose of this study was to determine the appropriate methods of breaking seed dormancy, level of water uptake, and the influence of gibberellic acid on the early growth of Tamarindus indica seedlings. The scarification methods used included: concentrated sulphuric acid (H2SO4), manual scarification, flaming, hot and cold water treatments. The experiments were conducted in the laboratory on Petri dishes and in potting media. GA3 was used to optimize the production of seedlings by spraying the solution on the foliage. The treatment for 10 minutes with Conc. H2SO4 gave the maximum germination percentage and water uptake (80.41%). GA3 enhances the growth of the seedlings by increasing the height, the number of leaves and stem girth at eight weeks after sowing. In conclusion, all the scarification treatments applied to the seeds of T. indica proved effective. The ten (10) minutes treatment with concentrated H2SO4 was the best treatment for breaking seed dormancy of T. indica. And gibberellic acid enhanced early and fast seedling growth as it increase height, number of leaves and stem girth of T. indica.
This study was carried out on the mycoflora associated with seeds of different citrus species. Citrus seed material was collected from districts of Punjab, i.e. Multan, Sargodha and Khanpur. Standard methods were applied for the isolation and identification of fungi. A total of 11 fungi including Aspergillus fumigatus, Aspergillus flavus, Dreschslera tetramera, Alternaria alternata, Curvularia lunata, Macrophomina phaseolina, Aspergillus niger, Fusarium solani, Fusarium moniliforme, Rhizopus and Penicillium spp were isolated from the seeds of citrus. For control of isolated seed-born fungi, 3 recommended fungicides such as Ridomil Gold, Bavistin, Score and two chemical Salicylic acid and Boric acid, were used at 20, 30, 40 mg/10 mL and 5, 6, 7 μL/10 mL, respectively and chemical with 20, 30, 40 mg/10 mL. All these fungicide and chemicals significantly reuced with population of all fungi present in naturally infected seed samples. Ridomil Gold and Salicylic acid were found to be the best for the control of se d-born fungi of citrus seed at 40 mg/10 mL. The isolation and identification of different mycotoxins is essential to study health status of the citrus consumers and to safeguard the standards of WTO.
Turbidity and Colour Reduction of Pond Water Using Extracts of Diospyros mesp...AZOJETE UNIMAID
The study examined the coagulation performance of leaves and root bark extracts of Diospyros mespiliformis, Mitragyna inermis, Piliostigma reticulatum and Tamarindus indica. The plant materials were collected along River Kubwa in Damboa local Government area of Borno State, Nigeria. The crude extracts of these plant materials were prepared based on soxhlet method using soxhlet solvent extractor. About 400mg of each pulverized sample was used for the extraction process. Three (3) liters of distilled water was used for the extraction process. The extracts were each added to highly turbid (422 NTU) pond water samples at various dosages of 60, 75, 90, 105, 120, and 135mg/l and a conventional coagulation-flocculation jar tests were conducted. The nature of the flocs formation for all the plant extracts were observed to be smoky rather than aggregating into denser masses and resulted in turbidity and colour removal efficiencies of 0.71-18.57% and 1.17-22.38% respectively. The coagulation activities were subjected to T-Test Analysis and it indicated that there was statistically (P< 0.05) significance difference at different concentrations. The results also revealed slight decrease in pH in the range of 8.1-7.55 exhibited by all the extracts. From the results of the turbidity and colour removal efficiencies, it can be concluded that these plant materials have the potential of improving the quality of pond or surface water (which most rural communities in developing countries depend for many purposes) that are heavily turbid; However, for them to perform effectively, a coagulant aid is required.
Evaluation of the Activity of Insecticides Plants in the Far North Region of ...IJEAB
This study proposes to assess the activity of insecticide plants in the far North region of Cameroon. The leaves or bark of four local plants (Azadirachtaindica, Boswelliadalzeilii, Khayasenegalensis and Ocimumcanum) were harvested, dried and powdered for the formulation of insecticidal chopsticks at different doses. Toxicity tests have been conducted on adult culicidae mosquitoes by fumigation. They reveal low levels of mortality after 15 minutes of exposure to the smoke of the chopsticks. Remanence due to chopsticks smoke leads to high rates of mortality after 6 and 24 hours of exposure. Mortality rates increase with the dose of each vegetable powder. Lethal doses were calculated 6 hours after exposure for each plant powder. Those of the leaves of Azadirachtaindica proved to be the most efficient thus with the lowest LD50 value of 36.14%. These vegetable powders can be used as natural insecticides instead of chemical insecticides.
Pharmacological activity of the methanolic extract of sea urchins against esc...Innspub Net
This study elucidated the pharmacological potential of sea urchins using methanol as extracting medium. The antibacterial potential was evaluated using the paper disc method and zone of inhibition against Escherichia coli and Staphylococcus aureus was measured. Antioxidant properties of sea urchins were evaluated using DPPH radical scavenging assay. Three species of sea urchin randomly collected along the intertidal zone of Diguisit, Baler Aurora were identified using diagnostic keys by the National Museum of the Philippines and they were identified as follows; Echinothrix diadema, Echinometra mathaei, and Echinometra oblonga. E. diadema recorded the highest diameter zone of inhibition against E. coli and S. aureus after 24 hours of incubation with 11.03 ± 1.75mm and 13.52 ± 1.13mm respectively while E. mathaei only inhibited S. aureus with zone of inhibition of 9.27 ± 2.06mm in 24 hours of incubation as well. As the zone of inhibition prolongs, the zone of inhibition decreases as observed in 48 hours of incubation. E. oblonga did not show inhibitoy effect, however it recorded the highest radical scavenging activity with 64.46% among the three species of sea urchins. This was followed by E. mathaei (51.52%) and E. diadema (37.38%). All collected species manifested antioxidant potential. Based on the results, the collected species of sea urchins has a pharmacological potential.
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
The papers for publication in The International Journal of Engineering& Science are selected through rigorous peer reviews to ensure originality, timeliness, relevance, and readability.
Efficacy of aqueous extracts of Aloe zebrina Baker, Capsicum annum L. and Mel...Innspub Net
Low cotton yields in Zimbabwe are mainly caused by infestation by high numbers of insect pests, diseases, poor agronomic practices and poor season quality. Aphids cause considerable yield loss in cotton. Control methods implemented for aphids include cultural approaches, chemical and biological control and use of biopesticides. Synthetic pesticides used by farmers have problems of environmental contamination, toxicity to non-target organisms, resistance by pests and toxicity to humans among others. Plant extracts (PEs) are suitable alternatives to synthetic pesticides. Three aqueous PEs at 10 and 20%v/v application rates, distilled water (no spray) and acetamiprid were evaluated against aphids. The 3×2 factorial + 2 experiments were laid out in an RCBD in the field and CRD for laboratory experiments. Leaves of Aloe zebrina and Melia azedarach and Capsicum annum fruits were dried and ground into powder. Water extracts were prepared and used at 10 and 20%v/v and compared with synthetic insecticide and distilled water in the laboratory. In field evaluations distilled water was replaced by unsprayed plots. All three PEs had insecticidal properties against aphids. During laboratory experiments C. annum 20%v/v killed 64.75% aphids and C. annum 10%v/v killed 63.85% aphids and they were significantly (p=0.007) different from other treatments after correcting for mortality. An average of 80.3% and 72.2% aphids were controlled by C. annum 20%v/v and A. zebrina 20%v/v during field trial, these were significant (p<0.001). A. zebrina extracts had both repellence and contact toxicity against aphids. All the PEs are suitable alternatives to aphicides for aphid management.
MICROORGANISMS ASSOCIATED WITH THE SPOILAGE OF CUCUMBER, GARDEN EGG AND PAWPA...paperpublications3
Abstract: A total of nine cucumbers, each of Garden egg and pawpaw samples were collected from Wurukum, High level and Wadata markets and cultured on appriopate agar, for colony count and isolation of bacteria according to their cultural and biochemical characteristics. The results revealed that garden egg from High Level Market had the highest bacterial count (1.9x105cfu/g) and the least was pawpaw from High Level Market (1.1 x 105 cfu/g). However, it was not statistically significant. The bacteria isolated were; Propianol bacteria (23.3%), Escherichia coli (16.6%), Staphylococcus aureus (36.7%), Bacillus spp (10.0%) and Corynebacteria (13.3%). The fungal isolates were Aspergillus flavus (10%), Aspergillus fumigatus (20%), Aspergillus nidulus (10%), Aspergillus terreus (20%) and mucor (40%). The result of this study shows fruits sold in Wurukum, High Level Market and Wadata Market are contaminated and may cause harm to consumers, so measures such as proper handling should be taken to control the contamination of these fruits.
Assessment of Endophytic Fungal Flora Responsible for Plant Growth Promotion...Sryahwa Publications
The present paper discusses the highest colonization of fungal endophytes as Alternaria speciesin comparison with Colletotrichumspecies and Fusarium species in all three plants Pongamia pinnata, Securinega leucopyrus and Rhus mysorensis. These endophytic fungi protect these plants from various
environmental factors such as temperature, moisture and other environmental factors.
The experiment was conducted at the experimental farm and laboratory of Institute of
Sustainable Agrotechnology, University Malaysia Perlis, Padang Besar, Perlis, Malaysia, with the objective
toinvestigate the inhibitory and stimulatory effects of aqueous extract of mungbean on seed germination and
seedling growth of three crop species, mungbean, sweet corn and okra. Different treatments of mungbean
aqueousextracts (vegetative fresh, vegetative after 2 weeks drying, vegetative after 4 weeks drying, flowering
fresh, flowering after 2 weeks drying, flowering after 4 weeks drying, flowering fresh, flowering after 2 weeks
drying, flowering after 4 weeks drying, maturity fresh, maturity after 2 weeks drying, maturity after 4 weeks
drying and water as control) were used to test their effect on the test species. The experiment was randomly
distributed and according to Completely Randomized Design(CRD) with five replicates. The results showed the
fresh vegetative aqueous extract of mungbean had a significant effect (stimulatory) on germination percent and
growth parameters such as number of root, root length and shoot height, of the three crop species. The study
revealed that the aqueous extract of mungbean have different effects (inhibitory and stimulatory) on the
seedlings and the mode of action depends on the associated plant species.Our results suggest that the aqueous
extract of mungbean from the different growth stages and drying periods have an allelopathic effect.
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Abstract— An efficient and relatively simple regeneration system was developed for an elite cultivar of Chinese Jujube, a perennial tree, by culturing young twig segments as explants from 8-15 year old trees. The twig segments were disinfected by submerging them in 1% sodium hypochlorite (NaOCl) for 15 min with 3 min vacuum. Calli developed from both ends of the twig segments on half-strength MS medium supplemented with sucrose and BA or BA and NAA in combination. The frequency of shoot formation from calli was higher than 80% when the explants were placed on the half - strength MS medium supplemented with BA (2.581 μM) and NAA (2.685 μM). Roots were produced from adventitious buds for 90% of the regenerated shoots when they were placed on the MS medium supplemented with 4.920 μM IBA and 5.708 μM IAA. After transplanting to soil, 82% of the regenerated seedlings survived when they were covered with glass containers to maintain humidity. The results suggest that Chinese jujube can be reproduced and multiplied using organogenesis with the appropriate explant and culture medium.
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Effects of acalypha wilkesiana leaf extract, hot and boiling
1. Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.18, 2014
Effects of Acalypha wilkesiana Leaf Extract, Hot and Boiling
Water on Plantain Growth Trend and Soil Nematode Densities
M. Omolara Olaniyi1
Department of Crop, Soil and Pest Management, School of Agriculture and Agricultural Technology,
Federal University of Technology, Akure, Ondo State, Nigeria
The study was funded in part by the International Foundation for Science through research grant number
C/3859-1. Olumide Alabi gave technical assistance with data collection.
Abstract
An experiment was laid out at the Teaching and Research Farm of the Federal University of Technology, Akure
to investigate the trend in plantain growth response and nematode population density build up in response to
paring, red acalypha leaf extract, hot water and boiling water dip. Above ground plant growth parameters were
assessed 55, 84 and 111 days after planting, approximately 8, 12 and 16 weeks respectively. Population densities
of plant parasitic nematodes in root rhizosphere at these dates were also investigated. The results indicated that
paring enhanced plantain resistance to plant parasitic and edaphic stressors. In a holistic approach, paring and
further treating pared suckers in red acalypha leaf extract for 5-15 mins conferred protection against plant
parasitic nematodes and liming effect on the soil. This supposed liming effect merits further investigations.
However, pre-plant dip of pared suckers in red acalypha leaf extract for 20 mins resulted in stress on the plant,
which encouraged high nematode density build up and concomitant parasitism.
Keywords: Helicotylenchus multicinctus, Pratylenchus coffeae, Radopholus similis, plantain, red acalypha, hot
water treatment, plant extract
1. Introduction
Plant parasitic nematodes are major constraints to the production of plantain (Musa spp., AAB-group) in Nigeria.
With reference to the, black sigatoka leaf disease and the banana weevils, the nematode species, Pratylenchus
coffeae and Radopholus similis are considered the key pathogens causing great damage to plantain in Nigeria
(Speijer et al., 2001). P. coffeae is strongly associated with root damage and plant toppling over, which are
strong indices of plant parasitic nematode damage. The species has been ranked the number one constraint to
plantain production in Nigeria (Olaniyi, 2011). The plant parasitic nematodes species most frequently associated
with plantain in Nigeria are Helicotylenchus multicintus, Hoplolaimus pararobustus, Meloidogyne spp.,
Pratylenchus spp. and Radopholus similis (Rotimi et al., 1999; Speijer et al., 2001).
A complex of Radopholus similis, Helicotylenchus dihystera, H. multicinctus, Hoplolaimus pararobustus and
Meloidogyne species in South eastern Nigeria cause on average, 50% loss in plantain production in Nigeria
depending on the cultivar type and cultural practices employed (Olaniyi, 2011). Pratylenchus coffeae was
reported to be important in southwestern Nigeria, yet in spite of its seriousness, it damage potential in
Southwestern Nigeria is yet to be quantified and described.
These damaging nematodes are introduced to new and otherwise clean fields through infested soil adhering to
planting materials and also infected roots of sucker planting materials (Speijer et al., 2001, Rotimi and Opadare,
2006). This makes sanitation an important nematode management principle in plantain. To improve root and
rhizome health, sucker planting materials are often pared (i.e. removal of adhering soil and peeling of the skin of
the rhizome in order to expose infected tissues, remove where possible before planting or discard materials with
serious infection (Blake, 1961). However, farmers do not feel comfortable with this procedure and are slow to
adopt it because they often believe that such pared suckers would not survive and if they do would have low
productivity. In order for farmers to adopt this cultural practice, the benefits of paring need to be demonstrated.
Also in developing effective environmentally friendly interventions, several options are considered. Acalypha
wilkesiana (red Acalypha) leaf extract has been demonstrated to be effective against plant parasitic nematodes
(Rotimi and Moens, 2005) and documented as effective plantain root health enhancer. In order to develop an
effective control tactic for plant parasitic nematodes on plantain an integrated management package should be
developed. Therefore, this study investigated the efficacy of combining paring with red Acalypha leaf extract at
different exposure duration, hot water or boiling water treatments in the control of plant parasitic nematodes on
plantain, cultivar Agbagba, in P. coffeae endemic Southwestern Nigeria.
105
2. Materials and Methods
2.1 Site Description and Field Layout
1 Current Address: Biology Unit, National Open University of Nigeria, 14/16 Ahmadu Bello Way, Victoria Island, Lagos,
Lagos State. E-mail: molaniyi@noun.edu.ng
2. Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.18, 2014
The trial was conducted at the Teaching and Research Farm (Crop section) of the Federal university of
Technology, Akure. Akure lies within the Tropical rainforest belt between latitude 5oN and longitude 150E of the
equator, with an annual mean temperature of about 27oC.the dry season is usually witnessed in Akure between
November and March, while the rainy season ranged from April to October.
The experimental site covered a total area of 960m2. Previously, the site was used for a mulch trial to study the
vegetative response of plantain to two organic mulch types. The experiment had been terminated five months
earlier and left to re-vegetate naturally before the site was opened for the present study. The trial was arranged in
a completely randomized design (CRD) of eight treatments in all and ten replicates per treatment. The site was
slashed and burned before marking out and establishing the field. The spacing used was 3 metres between the
rows and 2 metres within the rows, there were 10 suckers per row and eighty suckers in all for the trial.
2.2 Preparation of Red Acalypha Leaf Extracts and Sucker Treatment
Plantain (cultivar Agbagba) suckers were sourced from within the vicinity of the Teaching and Research Farm of
the University (Crop section), while the red Acalypha was sourced from Owena, a suburb of Akure. There were
eight treatments in the experiment including the controls. The treatments were: pared control (T1), pared suckers
with 5 mins dip in Acalypha extract (T2), pared suckers with 10 mins dip in Acalypha extract (T3), pared suckers
with 15 mins dip in Acalypha extract (T4), pared suckers with 20 mins dip in Acalypha extract (T5), Hot water
treatment at 52oC for 20 mins (T6), Boiling water treatment at 100oC for 30 sec. (T7) and Non-pared control
(T8).
Air-dried leaves of red Acalypha plant were pulverized and 100g of the powder was dissolved in 9 litres of cold
water in four different buckets each. A wooden rod was used to stir the mixture to ensure homogenization after
which they were left to stand for 30 mins. The buckets were respectively labelled as 5, 10, 15 and 20 minutes
with a marker to avoid mix-up. Thereafter, 10 suckers were dipped into each of the buckets and they were left to
stand for the duration on each bucket’s label. The suckers were removed and left to air dry under shade
afterwards.
For the hot water treatment, 10 suckers were dipped in water at 52oC ±2oC for 20 mins and they were removed
and left to cool for about 24 hours before planting, while for the boiling water treatment, 10 suckers were dipped
in water at 100oC for 30 sec. Suckers were planted on 21st December, 2006. Due to cessation of rainfall during
this period of the year, manual irrigation of the plant once in two days was adopted until the resumption of
rainfall in late March, 2007.
2.3 Above Ground Plant Growth Parameters
Growth parameters were taken on the above ground parts of the sprouted plants at 55, 84 and 111 days after
planting (DAP), giving approximately 8, 12 and 16 weeks after planting (WAP) respectively. Data taken
included the height of the pseudostem of the mother plant from the surface to the point of emergence (axils) of
the youngest leaf, the girth of the pseudostem at soil level, number of functional (green) and non-functional (dry)
leaves. A leaf was considered functional when at least 75% of the leaf area is green and non-functional if
otherwise (Rotimi et al., 2004b). The length and the width of the youngest leaf were measured: the length of the
youngest leaf opened was taken from the stalk to the leaf apex while the width was taken from the widest portion
of the leaf. The leaf area was calculated as length x width x 0.83 (a constant) according to Obiefuna and Ndubizu
(1979).
2.4 Nematode Extraction and Identification
Soil samples were taken from the rhizosphere of each plant at 8, 12 and 16 WAP. One hundred ml of each
sample was measured unto a serviette placed in a plastic sieve. The sieve was then placed in a plastic plate and
water was carefully added to the plate until the soil on the serviette-covered sieve appeared moist on top
(modified Baermann tray). The setup was left to stand for 24 hours after which the resulting suspension was
decanted, left to stand for 4 hours afterwards and then reduced to 30ml with a syringe.
Plant parasitic nematodes were identified to species level with the light microscope and all developmental stages
of the nematode species were counted, except for the root knot nematode which was identified only to genus
level and only vermiform juveniles and males that could be extracted with the extraction technique were
counted. The total of all the developmental stages counted was presented in each case. Densities of the
nematodes were estimated and presented per litre of soil.
2.5 Soil Chemical Analysis
At time of field establishment, soil samples were taken from each replicate hole and bulked per treatment for
physico-chemical analysis. Soil samples were also taken at termination of the experiment, 16 WAP. Soil
chemical analysis was carried out in the soil Analytical laboratory of the Department of Crop, Soil and Pest
106
3. Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.18, 2014
Management of the Federal University of Technology, Akure. Soil properties determined included pH, Nitrogen,
cations such as Ca2+, Mg2+ and K+ content. Others were Phosphorus, Sodium, organic matter, and organic carbon.
3. Results
3.1 Effects of Treatments on Plantain Growth Pattern across the Sampling Dates
The effect of the treatments on plant height relative to time is presented in Figure 1(a - h) the R2 value presented
on each graph is the coefficient of determination of each regression equation. The 5 mins dip in red acalypha leaf
extract (Figure 1b), 10 mins dip in red acalypha leaf extract (Figure 1c) and 30 sec dip in boiling water (Figure
1g) treatments exhibited linear trend in growth response over time. A quadratic polynomial functional trend in
height increase was observed with the 20 minutes dip in Acalypha extract treatment over time (T5), hot water
treatment and non-pared control which is the farmer’s method followed (Figure 1e, f, h). Only the pared (Figure
1a) and the 15 mins dip in Acalypha extract (Figure 1d) displayed a cubic polynomial trend in height increase
over time.
Pseudostem thickness increased at a linear rate over time only for the pared treatment (Figure 2a) while for the 5
mins dip in red acalypha leaf extract, pseudostem thickness over time responded with a quadratic polynomial
function (Figure 2b). All other treatments (Figure 2c - h) displayed a cubic polynomial trend in increase in
thickness over time.
In the number of functional leaves produced, the pared treatment followed a cubic polynomial function over time
(Figure 3a) while boiling water dip (Figure 3g) and the farmer’s material (Figure 3h) displayed a quadratic
polynomial trend from 55 to 111 days after planting. The number of green (functional) leaves produced in the 5,
10, 15 and 20 minutes dip in Acalypha extract treatments along side the hot water treatment exhibited a linear
response over the sampling dates (Figure 3b –f).
Ten, 15 and 20 min dip in red acalypha leaf extract as well as 30 sec dip in boiling water resulted in linear
response in leaf area expansion from 55 to 111 days after planting (Figure 4 c, d, e and g) while expansion trend
in leaf area in all the other treatments can be explained by the quadratic polynomial function.
107
4. Journal of Biology, Agriculture and Healthcare www.iiste.org
ISSN 2224-3208 (Paper) ISSN 2225-093X (Online)
Vol.4, No.18, 2014
25
20
15
10
5
a b
30
25
20
15
10
5
c d
25
20
15
10
5
e f
35
30
25
20
15
10
5
g h
18
16
14
12
10
8
6
4
2
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25
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5
Figure 1. Effects of red Acalypha extract, hot and boiling water treatments on height response of plantain from
55 to 111 days after planting.
108
Pared control
y = 7E-05x3 - 0.0155x2 + 1.0978x - 7.8614
R2 = 0.7274
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
5 minutes dip in Acalypha
extract
y = 0.0747x + 12.09
R2 = 0.9329
0
0 20 40 60 80 100 120
days after planting (days)
Height (cm)
10 minutes dip in Acalypha
extract
y = 0.1942x + 3.9017
R2 = 0.9773
0
0 20 40 60 80 100 120
days after planting (days)
15 minutes dip in Acalypha
extract
y = -8E-05x3 + 0.0208x2 - 1.6214x +
64.412
R2 = 0.2923
0
0 20 40 60 80 100 120
days after planting (days)
Height (cm)
20 minutes dip in Acalypha
extract
y = 0.003x2 - 0.3532x + 27.632
R2 = 0.7396
0
0 20 40 60 80 100 120
days after planting (days)
Height (cm)
Hot-water treatment
y = -0.0024x2 + 0.4299x + 1.4399
R2 = 0.6771 0
0 20 40 60 80 100 120
days after planting (days)
Height (cm) Boiling-water treatment
y = 0.0632x + 11.097
R2 = 0.7666
0
0 20 40 60 80 100 120
days af t er plant ing (days)
Non-pared control
y = -0.0042x2 + 0.5441x + 10.251
R2 = 0.6654
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
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Vol.4, No.18, 2014
109
10
8
6
4
2
a b
12
10
8
6
4
2
c d
12
10
8
6
4
2
e f
10
8
6
4
2
g h.
10
8
6
4
2
14
12
10
8
6
4
2
10
9.5
9
8.5
15
10
5
Figure 2. Effects of red Acalypha extract, hot and boiling water treatments on pseudostem girth of plantain
response from 55 to 111 days after planting.
Pared control
y = -0.0442x + 10.391
R2 = 0.945
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
5 minutes dip in Acalypha
extract
y = 0.0007x2 - 0.1269x + 13.265
R2 = 0.6359
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
10 minutes dip in Acalypha
extract
y = 2E-05x3 - 0.0052x2 + 0.3831x -
0.9432
R2 = 0.4287
0
0 20 40 60 80 100 120
days after planting (days)
Girth (cm)
15 minutes dip in Acalypha
extract
y = -9E-05x3 + 0.0233x2 - 1.8922x +
58.767
R2 = 0.6689
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
20 minutes dip in Acalypha
extract
y = -9E-05x3 + 0.0229x2 - 1.7801x + 51.469
R2 = 0.3556
0
0 20 40 60 80 100 120
days af t er pl ant i ng ( days)
Hot-water treatment
y = 3E-05x3 - 0.006x2 + 0.4357x -
0.9446
R2 = 0.4061
8
0 20 40 60 80 100 120
days af t er plant ing ( days)
Boiling-water treatment
y = -4E-05x3 + 0.0108x2 - 0.8369x +
28.418
R2 = 0.8299
0
0 20 40 60 80 100 120
days after planting (days)
Girth (cm)
Non-pared control
y = -0.0016x2 + 0.1399x + 10.737
R2 = 0.9049
0
0 20 40 60 80 100 120
days after planting (days)
Girth (cm)
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Vol.4, No.18, 2014
110
4.5
4
3.5
3
2.5
2
1.5
1
0.5
a b
8
7
6
5
4
3
2
1
c d
6
5
4
3
2
1
e f
6
5
4
3
2
1
g h
7
6
5
4
3
2
1
8
7
6
5
4
3
2
1
6
5
4
3
2
1
4.5
4
3.5
3
2.5
2
1.5
1
0.5
Figure 3. Effects of red Acalypha extract, hot and boiling water treatments on number of green leaves produced
by the plant from 55 to 111 days after planting.
Pared control
y = -0.001x2 + 0.1532x - 1.972
R2 = 0.887 0
0 20 40 60 80 100 120
days af t er plant ing ( d ays)
5 minutes dip in Acalypha
extract
y = 0.0511x + 0.04
R2 = 0.8296
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
10 minutes dip in Acalypha
extract
y = 0.0704x - 1.0638
R2 = 0.8859
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
15 minutes dip in Acalypha
extract
y = 0.069x - 0.9813
R2 = 0.9529
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
20 minutes dip in Acalypha
extract
y = 0.0597x - 1.0026
R2 = 0.9944
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
Hot-water treatment
y = 0.0545x - 0.4345
R2 = 0.9548
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
Boiling-water treatment
y = 0.0006x2 - 0.0677x + 5.6733
R2 = 0.5818
0
0 20 40 60 80 100 120
days after planting (days)
no. of green leaves
Non-pared control
y = -0.0013x2 + 0.2194x - 5.3017
R2 = 0.9004
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
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Vol.4, No.18, 2014
leaf area (cm2)
leaf area (cm2)
111
Pared control
y = -0.0835x2 + 12.06x - 190.27
R2 = 0.8809
300
250
200
150
100
50
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
a b
10 minutes dip in Acalypha
extract
y = 4.6715x - 80.986
R2 = 0.8613
500
400
300
200
100
0
0 20 40 60 80 100 120
days after planting (days)
leaf area (cm2)
c d
20 minutes dip in Acalypha
extract
y = 3.5363x + 16.008
R2 = 0.74
500
400
300
200
100
0
0 20 40 60 80 100 120
days after planting (days)
leaf area (cm2)
e f
Boiling-water treatment
y = 1.6598x + 143.12
R2 = 0.633
400
300
200
100
0
0 20 40 60 80 100 120
days after planting (days)
leaf area (cm2)
g h
5 minutes dip in Acalypha
extract
y = -0.0955x2 + 15.076x - 276.73
R2 = 0.7995
350
300
250
200
150
100
50
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
15 minutes dip in Acalypha
extract
y = 1.697x + 333.63
R2 = 0.5318
600
500
400
300
200
100
0
0 20 40 60 80 100 120
days af t er plant ing ( days)
Hot-water treatment
y = -0.1562x2 + 27.117x -
768.53
R2 = 0.7773
500
400
300
200
100
0
0 20 40 60 80 100 120
days after planting (days)
Non-pared control
y = -0.3791x2 + 57.51x -
1560.6
R2 = 0.7745
800
600
400
200
0
0 20 40 60 80 100 120
days after planting (days)
Figure 4. Effects of Acalypha wilkesiana leaf extract, hot and boiling water treatments on the leaf area growth
trend of plantain from 55 to 111 days after planting.
3.2 Effects of Treatments on Plant Parasitic Nematode Species and Densities Recovered
Three species of plant parasitic nematodes namely: Helicotylenchus multicinctus, Pratylenchus coffeae and
Radopholus similis were recovered from plantain rhizosphere in this study. At 55 days (8 weeks) after planting,
only the 5 minutes dip in extract (T2) had a combination of two species of nematodes namely Radopholus similis
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Vol.4, No.18, 2014
and Pratylenchus coffeae while P. coffeae was recovered under plantain in the other treatments except 20 mins
dip in extract and the not pared control (Figure 5a), at 84 days (12 weeks) after planting, the highest population
density and species combination was observed in the 20 minutes dip in extract (T5) (Figure 5b) while at 111
days (16 weeks), the nematode population recovered from the roots had reduced relatively to the earlier
population densities observed in preceding samplings (Figure 5c).
density/litre soil
112
nematode from soil 55 DAP
700
600
500
400
300
200
100
0
A
A
a
A
a
a
A
a
A
A a
a b
a
T1 T2 T3 T4 T5 T6 T7 T8
Treatments
density/litre soil
R.s
Praty
a
a b
c
nematode from soil 84 DAP
700
600
500
400
300
200
100
0
A
a a
a1
a1
a
A
T1 T2 T3 T4 T5 T6 T7 T8
Treatments
R.s
Praty
H.m
nematode from soil 111 DAP
1000
800
600
400
200
0
A
a a H.m
A
T1 T2 T3 T4 T5 T6 T7 T8
Treatments
density/litre soil
R.s
Praty
Figure 5. Effects of treatments on rhizosphere nematode densities at 55 (a), 84 (b) and 111 (c) days after planting
(DAP).
T1 = Pared control (no dip treatment), T2 = 5 mins dip in Acalypha extract, T3: 10 mins dip in Acalypha
extract, T4 = 15 mins dip in Acalypha extract, T5 = 20 mins dip in Acalypha extract, T6 = Hot-water treatment
for 20 mins, T7 = Boiling-water treatment for 30 sec, T8 = Non-pared control. Column fractions followed by
same letters (and numbers) are not significantly different at P≤0.05.
3.3 Effects of Treatments on Soil Chemical Properties
Chemical properties of the soil at pre-plant are presented on table 1 while table 2 presents the soil chemical
properties 111 days after planting. Ten and 15 mins dip in Acalypha leaf extract, and 30 sec dip in boiling water
depressed soil pH, while 5 and 20 mins dip in red Acalypha extract as well as 20 mins dip in hot water resulted
in slightly raised pH. The non-pared control, which represented the farmer’s method, also resulted in depressed
soil pH, while the pared suckers gave relatively constant pH. Generally, soil potassium content declined between
planting and termination of the experiment 111 days afterwards. Except for the 15 mins dip in Acalypha extract
treatments, soil phosphorus also declined from planting till end of experiment. Nitrogen content also declined
marginally from planting to termination of the experiment; except for the 20 mins hot water dip and 30 mins
boiling water dip treatments.
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Table 1. Pre-plant soil chemical properties in plots where the treatments shown were laid out
T1 = Pared control (no dip treatment), T2 = 5 mins dip in Acalypha extract, T3: 10 mins dip in
Acalypha extract, T4 = 15 mins dip in Acalypha extract, T5 = 20 mins dip in Acalypha extract, T6
= Hot-water treatment for 20 mins, T7 = Boiling-water treatment for 30 sec, T8 = Non-pared
control.
Treatment pH Mg Ca %OM %OC P Na K N
T1 5.87 2.30 2.90 11.31 6.55 1.42 7.13 1.13 0.31
T2 5.59 1.10 3.50 6.49 3.75 1.41 12.3 0.41 0.38
T3 5.73 0.70 3.40 10.02 5.72 1.10 13.3 0.56 0.21
T4 5.79 1.30 3.10 8.62 4.99 0.96 11.39 1.24 0.20
T5 5.45 1.10 2.00 9.93 5.75 0.88 10.52 0.87 0.32
T6 5.46 1.10 2.40 13.25 7.66 1.43 12.35 0.46 0.17
T7 6.08 0.80 2.90 9.87 5.71 2.41 10.96 0.36 0.27
T8 5.68 1.80 2.90 10.97 6.34 1.29 13.04 0.36 0.18
Table 2. Effects of paring, red acalypha leaf extract , hot and boiling water treatments of plantain
sucker planting materials on soil chemical properties 16 weeks after planting
T1 = Pared control (no dip treatment), T2 = 5 mins dip in Acalypha extract, T3: 10 mins
dip in Acalypha extract, T4 = 15 mins dip in Acalypha extract, T5 = 20 mins dip in
Acalypha extract, T6 = Hot-water treatment for 20 mins, T7 = Boiling-water treatment for
30 sec, T8 = Non-pared control.
Treatment pH Mg Ca %OM %OC P Na K N
T1 5.86 1.10 3.80 11.18 6.46 0.94 11.39 0.56 0.28
T2 5.94 0.10 3.80 11.31 6.54 0.83 14.43 0.87 0.31
T3 5.24 0.50 3.80 9.73 5.63 0.59 11.83 0.41 0.15
T4 5.60 1.70 2.70 10.69 6.19 0.90 15.13 0.36 0.18
T5 5.84 2.00 2.40 11.24 6.50 1.05 11.74 0.42 0.18
T6 5.52 0.70 2.50 9.38 5.43 1.24 12.17 0.29 0.31
T7 5.56 1.60 2.80 9.31 5.39 0.88 11.83 0.25 0.27
T8 5.55 0.50 2.80 9.31 5.31 1.04 10.26 0.27 0.13
4. Discussion
The linear response obtained for some of the growth parameters for 5, 10, 15 and 20 minutes dip in acalypha
extract, hot and boiling water treatments in this study connotes that the rate of change in the growth of the plant
in the treatments increased over time at a constant rate in spite of the prevailing harsh dry condition. Polynomial
functional relationships obtained in the non-pared control (Farmer’s method) might be an indication of the high
susceptibility of unpared planting materials to dry weather. This then suggests that paring the sucker before
planting enhanced the plant’s ability to withstand biotic and edaphic stress. Further treating the planting
materials with plant extract or hot water (52 ±2oC) also conferred some level of resistance to these stress factors.
Olaniyi (2014b, In Press) also reported functional growth trend in time for plantain in reaction to organic mulch.
The author noted that organic mulch enhanced the plants ability to withstand biotic stressors like plant parasitic
nematodes in the environment.
The effects of treatments in the present study on growth parameters such as the leaf area, number of functional
leaves, plant height and girth revealed that 15 minutes dip of planting materials in the extract showed some
promise in enhancing plant growth performance. However, it is not clear what this additional benefit of dipping
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pared suckers translates to in maintaining low nematode populations since the suckers that were only pared had
very low densities of P. coffeae (about 20/litre soil) and maintained no detectable level of nematodes
subsequently. Where as the highest density of P. coffeae recorded in this study was from the 15 mins dip in
extract, earlier report (Olaniyi, 2014a, In Press) showed that 15 min dip reduced nematode damage to root and
rhizome of plantain and also reduced nematode densities. In the present study 5 and 10 mins dip treatment
seemed to better reduce nematode population densities on plantain compared to the 15 mins dip. Generally,
Helicotylenchus multicinctus, Pratylenchus coffeae and Radopholus similis were the nematode species observed
from plantain roots and the rhizosphere in this study. There is need to monitor plant response to these treatments
and nematode development on plantain under these treatments till yield stage.
Although densities of the species in the rhizosphere were low with maximum of 100 H. multicinctus / litre, 600
P. coffeae / litre and 200 R. similis / litre, it is clear that densities had increased from the initial pre-plant
densities of 1 P. coffeae / litre soil and 2 H. multicinctus / litre soil. This shows that planting a host plant in a soil
would quickly increase the densities of the nematode species to a damaging level. It would be informative to
investigate the effect of these densities on root and rhizome damage of plantain. Before the choice of a plant to
put in a soil is made, it would be helpful to first do a diagnostic nematode assessment in order not to put in a host
crop in a field where a damaging species is prevalent. For instance, Speijer et al. (2001) established Pc as the
most damaging on plantain in Nigeria and the fact that it could persist in a field without the plantain host makes
its control a big challenge as this might signify that it has a wide host range. The trend in population densities
recovered from plantain rhizosphere is at variance with earlier record of H. multicinctus often in higher densities
than other species on plantain in Nigeria (Speijer et al., 2001; Rotimi et al., 2004b, c; Coyne et al., 2005).
Radopholus similis was not recovered from the soil before the establishment of this trial but was recovered from
the rhizosphere of plantain 55 days (8 weeks) after planting in 5 min dip in red acalypha leaf extract treatment
but subsequently, it was undetected in soil samples. This shows that first R. similis may not have as wide host
range as P. coffeae or probably it could not persist as long as P. coffeae in the absence of a preferred host hence
the absence of the species in the soil pre plant. The land had been fallowed for about 5 months before it was
opened up for this study. However, the presence and disappearance of the species at subsequent sampling date
supported Olaniyi’s (2011) submission that for accurate diagnosis, nematode sampling should be done across
seasons. The study reported here was done in the dry season and Rotimi et al. (2004a) similarly observed that the
densities of R. similis could decline to an undetected level in dry soil.
No specific trend emerged in plant parasitic nematode community structure under plantain in this study. This
suggests that several factors confound field studies and it would be useful to investigate nematode reactions to
these treatments in controlled environment. For instance in the unpared control treatment, no nematode was
detected in plantain rhizosphere 8 and 16 weeks after planting whereas at 12 weeks after planting P. coffeae was
detected at an average of 400 nematodes / litre soil. However under the pared treatment, only very low density of
Pc was recovered 8 weeks after planting. Subsequently no nematode was detected implying that it may suffice
for nematode control to simply pare plantain sucker planting materials before planting without any further
treatment. Olaniyi (2014a, In Press) also did not recover any plant parasitic nematode species from the roots of
pared plantain at those dates.
A number of factors could be responsible for the variations observed in the nematode incidence across the
sampling dates. The variations obtained in the result across the three samplings established the rationale and the
importance of sampling more than once in the diagnostic study of plant parasitic nematodes so that correct
assertion can finally be made (Olaniyi, 2011, 2014a, In Press). It is a common phenomenon in nematode
population dynamics, and that is why it is advisable to sample several times in time and season. Also, sampling
only the rhizosphere could give misleading information as the species recorded in this study are migratory
endoparasites and several factors could be responsible for their density fluctuation in the rhizosphere. To confirm
this, the study reported by Olaniyi (2014a, In Press) investigated root densities and a different pattern emerged.
Therefore, for a more holistic overview, it is important to sample both root and soil complementarily as this
would give more reliable information and better guide management decision. Other factors that may be
responsible for variations include the soil condition, environmental condition and availability of moisture in the
soil.
As expected, N, P & K values declined over time in this study. Results of this study revealed that introducing
soil from other sources with planting materials as with the farmer’s method of not paring may result in increased
soil acidity. But this would be dependent on the chemical status of the introduced soil. This is revealed by the
stable pH of soils from pared suckers, which had all adhering soils removed before planting. Dipping rhizomes
of planting materials in red Acalypha leaf extract for 5 or 20 minutes tended to reduce soil acidity. The liming
effect of red Acalypha should be further explored. Considering the overall effect of treatments on plant response
and nematode density, it would be safe to recommend paring as a useful cultural practice that farmers should be
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encouraged to engage in. Further treating pared suckers in red acalypha extract for 5-15 mins might confer
protection against plant parasitic nematode damage (Olaniyi, 2014a, In Press) and improve soil quality.
References
Blake, C.D. (1961), “Root rot of bananas caused by Radopholus similis (Cobb) and its control in New South
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Wales”. Nematologica 6, 295-310.
Coyne, D.L., Rotimi, M.O., Speijer, P., De Schutter, B., Dubois, T., Auwerkerken, A., Tenkouano, A. and De
Waele, D. (2005), “Effects of nematode infection and mulching on the yield of plantain (Musa spp.,
AAB-group) ratoon crops and plantation longevity in southeastern Nigeria”. Nematology 7(4), 531-541.
Obiefuna, J.C. and Ndubizu., T.O.C. (1979), “Estimating leaf area in plantain”. Scientia Horticulturae 11, 31-36.
Olaniyi, M.O. (2006), “Effect of organic mulch and root dips on the establishment and root health of plantain”.
Xvill Reunia International Acorbat Anais Proceedings Memorias, 321.
Olaniyi, M.O. (2011), “Plant Parasitic Nematode Constraint to Plantain production in Nigeria”. LAP Lambert
Publishing, Germany. September 2011. ISBN 978-3-8454-2312-8, 240pp.
Olaniyi, M.O. (2014a), “Effects of Acalypha wilkesiana leaf extract, hot and boiling water on plantain growth
response and nematode damage”. Nigerian Journal of Nematology, In Press.
Olaniyi, M.O. (2014b), “Functional trend in leaf and sucker production of plantain in response to paring and
organic mulch”. The International Journal of Science and Technoledge, In Press.
Olaniyi, M.O. and Opadare, S.O. (2006), “Effect of paring on the establishment and root damage of French
plantain suckers”. In: E. Soprano, F.A. Tcacenco, L.A. Litchemberg & M.C. Silva (Eds.). Banana: A
Sustainable Business. XVII ACORBAT International Meeting, Joinville, Brazil. 15-20 October 2006,
345.
Rotimi, M.O. and Moens, M. (2005), “Effect of leaf extracts of some herbs on the juveniles of Meloidogyne
incognita (Kofoid & White) Chitwood”. Nigerian Journal of Plant Protection 22, 95- 103.
Rotimi, M.O., Speijer, P.R. and De Waele D. (2004a), “Effect of soil moisture and organic matter levels on
plantain root reaction to Radopholus similis”. International Journal of Nematology 14(2), 122-128.
Rotimi, M.O., Speijer, P., De Waele, D. and Swennen, R. (2004b), “Effect of mulching on the response of
plantain (cv. Agbagba, Musa spp. AAB-group) to plant parasitic nematodes in Southeastern Nigeria. I:
Vegetative growth”. Nigerian Journal of Forestry. 34 (1), 61-71.
Rotimi, M.O., Speijer, P., De Waele, D. and Swennen, R. (2004c). “Effect of mulching on the response of
plantain (cv. Agbagba, Musa spp. AAB-group) to plant parasitic nematodes in southern Nigeria II:
Reproductive growth and yield” Nigerian Journal of Forestry. 34(2), 61-71.
Speijer, P. R., Rotimi, M.O. and De Waele, D. (2001), “Plant parasitic nematodes associated with plantain in
southern Nigeria and their relative importance compared with other abiotic constraints”, Nematologie 3,
423-436.
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