This document summarizes research characterizing two distinct E-cadherin-associated complexes in polarized epithelial cells: an apical zonula adherens (ZA) complex and a basolateral complex. The apical ZA complex, defined by the protein PLEKHA7, suppresses growth and contains proteins involved in cytoskeletal structure. The basolateral complex lacks PLEKHA7 and contains proteins associated with increased cell signaling, mobility, and cancer progression. Experiments provide evidence that the apical ZA complex regulates miRNA processing to suppress expression of transforming markers promoted by the basolateral complex, thereby maintaining epithelial homeostasis.
Structural Determinants of Drugs Acting on the Nav1.8 ChannelShahnaz Yusaf
1) The study investigated the roles of pore-lining residues in sodium channel Nav1.8 in drug binding and functional properties using alanine mutations and patch clamp recordings.
2) Mutations of some residues altered voltage dependence of activation and inactivation kinetics, indicating their importance in gating.
3) Mutations of residues I381A, F1710A, and Y1717A reduced affinity of tetracaine, while mutations of residues L1410A and F1710A altered affinity of compound A-803467, showing the involvement of these residues in drug binding.
Proinflammatory Consequences of FasL Expression in the HeartElizabeth Berry
This document summarizes research on the effects of transgenic expression of Fas ligand (FasL) specifically in heart muscle cells. The researchers generated several lines of transgenic mice with different copy numbers of a FasL transgene driven by a heart-specific promoter. They found that FasL expression in the heart caused mild leukocyte infiltration and interstitial fibrosis but, surprisingly, no cardiomyocyte apoptosis or necrosis. Instead, the hearts developed cardiac hypertrophy accompanied by upregulation of proinflammatory cytokines. The degree of proinflammatory changes correlated with transgene copy number, indicating a dose-dependent response to FasL expression level. This suggests that tissue-specific factors can modulate the proinflammatory effects of FasL.
1) Results so far suggest that several cell cycle genes, including ftsE and pleC, are differentially expressed in a ΔcbrA mutant compared to wild type, indicating they are regulated by factors disrupted in the mutant, such as CtrA.
2) Tracking expression of cell cycle genes in root nodules found that some, like MinC and CcrM, are expressed only early in nodule development while others, including RcdA, PleC, FtsE and CtrA, are expressed throughout nodule development and symbiosis.
3) Future work will characterize additional potential CtrA target genes, examine how their expression is altered in other mutants, and investigate expression
Ø Researchers used CRISPR/Cas9 nuclease and nickase to target and repair the c.456+4A>T mutation in the FANCC gene, which causes Fanconi anemia (FA), in human fibroblasts derived from an FA patient.
Ø Both nuclease and nickase mediated repair of the mutation, restoring normal FANCC gene function, though nickase was more efficient due to preferentially inducing the homology-directed repair pathway.
Ø Genome-wide analyses found no off-target effects, confirming the CRISPR reagents specifically targeted the intended FANCC locus. This provides proof-of-principle that CR
CRISPR/Cas9 was used as a gene editing tool to repair the FANCC c.456+4A>T mutation, which causes Fanconi anemia. The mutation was corrected in skin fibroblasts from a patient with the mutation. Tests at the DNA, RNA, and protein levels showed correction of the mutation. No off-target effects were observed. The nickase version of CRISPR/Cas9 was more efficient and reliable than the nuclease version for correcting the mutation. This demonstrates the potential of CRISPR/Cas9 for treating genetic diseases like Fanconi anemia by directly correcting mutations.
This document summarizes a study examining the role of the long non-coding RNA Saf and splicing factor 45 in regulating alternative splicing of the Fas pre-mRNA and production of soluble Fas protein. The main findings are:
1) Saf is predominantly localized to the nucleus of cells. Overexpression of Saf in cell lines resulted in minimal effects on global gene expression.
2) Saf interacts with Fas pre-mRNA and splicing factor 45. This interaction promotes the skipping of exon 6 in the Fas pre-mRNA, leading to increased production of soluble Fas protein.
3) Soluble Fas protein protects cells from Fas ligand-induced apoptosis by binding to the ligand and blocking
1) The study explores how the transcription factor KLF1 regulates the gene encoding delta-aminolevulinic acid dehydratase (Alad), the first enzyme in the heme biosynthesis pathway.
2) Using a cell line model where KLF1 can be induced, the study finds that KLF1 significantly increases Alad mRNA and protein levels by increasing transcription from the Alad promoter.
3) In contrast, the study finds little effect of KLF1 on mRNA levels of the genes encoding two other early heme biosynthesis enzymes, ALAS2 and PBGD, suggesting KLF1 specifically regulates Alad transcription.
What is chromatin remodelling? Types of Chromatin remodelling. How chromatin behave during plant stress and application of chromatin remodelling for crop improvement with examples.
Structural Determinants of Drugs Acting on the Nav1.8 ChannelShahnaz Yusaf
1) The study investigated the roles of pore-lining residues in sodium channel Nav1.8 in drug binding and functional properties using alanine mutations and patch clamp recordings.
2) Mutations of some residues altered voltage dependence of activation and inactivation kinetics, indicating their importance in gating.
3) Mutations of residues I381A, F1710A, and Y1717A reduced affinity of tetracaine, while mutations of residues L1410A and F1710A altered affinity of compound A-803467, showing the involvement of these residues in drug binding.
Proinflammatory Consequences of FasL Expression in the HeartElizabeth Berry
This document summarizes research on the effects of transgenic expression of Fas ligand (FasL) specifically in heart muscle cells. The researchers generated several lines of transgenic mice with different copy numbers of a FasL transgene driven by a heart-specific promoter. They found that FasL expression in the heart caused mild leukocyte infiltration and interstitial fibrosis but, surprisingly, no cardiomyocyte apoptosis or necrosis. Instead, the hearts developed cardiac hypertrophy accompanied by upregulation of proinflammatory cytokines. The degree of proinflammatory changes correlated with transgene copy number, indicating a dose-dependent response to FasL expression level. This suggests that tissue-specific factors can modulate the proinflammatory effects of FasL.
1) Results so far suggest that several cell cycle genes, including ftsE and pleC, are differentially expressed in a ΔcbrA mutant compared to wild type, indicating they are regulated by factors disrupted in the mutant, such as CtrA.
2) Tracking expression of cell cycle genes in root nodules found that some, like MinC and CcrM, are expressed only early in nodule development while others, including RcdA, PleC, FtsE and CtrA, are expressed throughout nodule development and symbiosis.
3) Future work will characterize additional potential CtrA target genes, examine how their expression is altered in other mutants, and investigate expression
Ø Researchers used CRISPR/Cas9 nuclease and nickase to target and repair the c.456+4A>T mutation in the FANCC gene, which causes Fanconi anemia (FA), in human fibroblasts derived from an FA patient.
Ø Both nuclease and nickase mediated repair of the mutation, restoring normal FANCC gene function, though nickase was more efficient due to preferentially inducing the homology-directed repair pathway.
Ø Genome-wide analyses found no off-target effects, confirming the CRISPR reagents specifically targeted the intended FANCC locus. This provides proof-of-principle that CR
CRISPR/Cas9 was used as a gene editing tool to repair the FANCC c.456+4A>T mutation, which causes Fanconi anemia. The mutation was corrected in skin fibroblasts from a patient with the mutation. Tests at the DNA, RNA, and protein levels showed correction of the mutation. No off-target effects were observed. The nickase version of CRISPR/Cas9 was more efficient and reliable than the nuclease version for correcting the mutation. This demonstrates the potential of CRISPR/Cas9 for treating genetic diseases like Fanconi anemia by directly correcting mutations.
This document summarizes a study examining the role of the long non-coding RNA Saf and splicing factor 45 in regulating alternative splicing of the Fas pre-mRNA and production of soluble Fas protein. The main findings are:
1) Saf is predominantly localized to the nucleus of cells. Overexpression of Saf in cell lines resulted in minimal effects on global gene expression.
2) Saf interacts with Fas pre-mRNA and splicing factor 45. This interaction promotes the skipping of exon 6 in the Fas pre-mRNA, leading to increased production of soluble Fas protein.
3) Soluble Fas protein protects cells from Fas ligand-induced apoptosis by binding to the ligand and blocking
1) The study explores how the transcription factor KLF1 regulates the gene encoding delta-aminolevulinic acid dehydratase (Alad), the first enzyme in the heme biosynthesis pathway.
2) Using a cell line model where KLF1 can be induced, the study finds that KLF1 significantly increases Alad mRNA and protein levels by increasing transcription from the Alad promoter.
3) In contrast, the study finds little effect of KLF1 on mRNA levels of the genes encoding two other early heme biosynthesis enzymes, ALAS2 and PBGD, suggesting KLF1 specifically regulates Alad transcription.
What is chromatin remodelling? Types of Chromatin remodelling. How chromatin behave during plant stress and application of chromatin remodelling for crop improvement with examples.
Genome-wide characterization of AP2/ERF and HSP 90 gene family in select legumesICRISAT
This study characterized transcription factor (AP2/ERF) and molecular chaperone (HSP 90) genes in five legumes: chickpea, pigeonpea, common bean, medicago, and lotus. It identified 147-179 AP2/ERF genes across the five legumes, which were classified into ERF, DREB, AP2, and other subgroups. Fewer HSP 90 genes were identified, ranging from 5-7 per species. Gene structures, phylogenies, and expression under stress provided insights into the roles and evolution of these gene families in legumes.
1. Depletion of the histone acetyltransferase KAT2A reduces the colony-forming potential of normal hematopoietic stem cells, but does not alter their lineage bias. This reinforces other data showing KAT2A depletion does not cause a global downregulation of lineage-specific genes.
2. KAT2A depletion may accelerate stem cell differentiation, leading to an apparent reduction in proliferation. This is supported by an association between "transient clones" and KAT2A knockdown.
3. Further single-cell studies are proposed to better understand how KAT2A impacts cell fate transitions and heterogeneity between sister cells, including analyzing lineage marker expression and first division kinetics through daughter cell
This document provides a curriculum vitae for Devanand Kumar that summarizes his education and work experience. He received a PhD in Microbiology from the University of Delhi, where he identified and characterized histone acetyltransferases in Leishmania donovani. As a post-doctoral researcher, he further characterized the histone acetyltransferase HAT3 in L. donovani and found it plays a role in histone deposition, DNA damage response, and mediates PCNA acetylation and degradation after UV exposure. He has over 10 years of experience in molecular and cellular biology techniques and currently works as a research scientist at Premas Biotech Pvt. Ltd.
The document summarizes a study that found:
1) Interleukin-12 (IL-12) in dendritic cells traffics through late endocytic vesicles marked by the SNARE protein VAMP7.
2) Dendritic cells from VAMP7 knockout mice show partially impaired secretion of bioactive IL-12/p70 dimer.
3) At the immune synapse between dendritic cells and T cells, IL-12 containing vesicles rapidly redistribute and their release is entirely dependent on VAMP7, leading to reduced T cell acquisition of effector functions when stimulated with VAMP7 knockout dendritic cells.
This document summarizes a comparative genome analysis that identified five distinct subfamilies of sortase enzymes in gram-positive bacteria. Sortases are enzymes that anchor surface proteins to the cell wall by recognizing a cell wall sorting signal on the proteins. The analysis examined 72 bacterial genomes and identified 176 sortase homologs that clustered into six subfamilies based on sequence similarity, including two previously known subfamilies (SrtA and SrtB) and three new subfamilies numbered 3, 4, and 5. A hidden Markov model was used to further characterize the subfamilies. The analysis aims to predict which specific sortase enzymes are responsible for anchoring which surface proteins based on the sorting signal motifs.
The complement system is a series of more than 30 proteins that interact in a precise order to enhance host defense. It can be activated through three pathways: the classical pathway involves antibody binding and nine proteins; the alternative pathway is antibody-independent; and the lectin pathway involves mannose-binding lectin binding to pathogens. Complement activation results in cytolysis of target cells, opsonization to enhance phagocytosis, chemotaxis to recruit immune cells, and anaphylaxis.
ShRNA-specific regulation of FMNL2 expression in P19 cellsYousefLayyous
This video encompasses all the steps and data produced for my graduation project in BSc in Biopharmaceutical science. During the course of the project we modified mammalian cells using Short Hairpin RNA to inhibit the correct function of the cytoskelleton. In this way we studied the importance of FMNL2 for the activation and regulation of actin fibers. Among the methods used are Flourescent microscopy, mamallian cell culture, cloning and flow cytometry.
This research article investigates how plant ribosome-inactivating proteins (RIPs) induce cellular stress responses in human cancer cells. The researchers found that two human cancer cell lines exposed to three RIPs - ricin, riproximin and volkensin - activated the unfolded protein response (UPR), a stress response pathway in the endoplasmic reticulum. This suggests the UPR induction better explains the cellular effects of RIPs, as apoptosis was induced even when some protein translation was still occurring due to ribosomal damage. The study provides new insights into the molecular mechanisms by which RIPs exert their toxic effects on cells.
The document discusses hypoxia-inducible factor 1 (HIF-1), which promotes tumor growth through various target genes involved in processes like angiogenesis and drug resistance. The HIF-1α subunit is regulated by oxygen levels and targeted for degradation, making it an important target for cancer therapy. The authors designed G-rich oligonucleotides (ODNs) that form G-quartet structures to selectively target and inhibit HIF-1α. Two lead compounds, JG243 and JG244, decreased HIF-1α and HIF-2α levels and inhibited related proteins without affecting other factors. These JG-ODNs induced degradation of HIF-1α and HIF-2α in
Vitamin C inhibits FAS-induced apoptosis in monocytes and U937 cells by reducing caspase activity and ROS levels. The study found that loading cells with high intracellular concentrations of vitamin C through DHA administration blocked FAS-mediated apoptosis. Vitamin C's primary effect was inhibiting caspase-8 activation, with a separate impact on preserving mitochondrial membrane integrity and reducing ROS. This illuminates vitamin C's role in modulating redox signaling in FAS-induced apoptosis of immune cells.
This document summarizes a study investigating the role of a Plasmodium falciparum S33 proline aminopeptidase (PfPAP) in changes to host red blood cell deformability. The key findings are:
1) PfPAP contains a predicted protein export element suggesting it is exported into infected red blood cells. In silico modeling and recombinant protein studies confirmed PfPAP is a proline aminopeptidase.
2) Genetic deletion of PfPAP in P. falciparum led to an increase in the deformability of infected red blood cells and reduced adherence of infected cells to the endothelial cell receptor CD36 under flow conditions.
3) These results suggest PfP
J. biol. chem. 2016-shao-jbc.m116.724401andrei andrei
FBXO3 promotes ubiquitylation and transcriptional activity of AIRE. The study found that the E3 ubiquitin ligase FBXO3 interacts with phosphorylated residues on AIRE and promotes its ubiquitylation. This post-translational modification increases the interaction between AIRE and P-TEFb, potentiating their transcriptional activity on tissue-specific antigen genes in the thymus. Knockdown of FBXO3 decreased ubiquitylation and transcriptional activity of AIRE.
Bacteria Induced Cryptic Meroterpenoid Pathway in Pathogenic Aspergillus fumi...Debanjan Chatterjee
The document summarizes a presentation on inducing a cryptic meroterpenoid pathway in the pathogenic fungus Aspergillus fumigatus through co-cultivation with the actinomycete Streptomyces rapamycinicus. Co-cultivation led to the activation of a previously silent polyketide synthase gene cluster and the production of novel prenylated polyketides, including Fumicyclines A. Deletion of the polyketide synthase gene confirmed its involvement in biosynthesis. While co-cultivation induced pathway expression, inhibition of histone acetyltransferase did not, suggesting the bacterium alters fungal epigenetic regulation. Understanding secondary metabolism in A. f
1) The document describes a study identifying new genes involved in gliding motility in Flavobacterium johnsoniae.
2) Transposon mutagenesis of an F. johnsoniae sprB mutant identified 8 mutants with increased phage resistance and reduced motility. 4 mutants had transposon insertions in remA, which encodes a cell surface protein with a lectin domain.
3) RemA was shown to localize to the cell surface and move rapidly along the cell surface, suggesting it acts as a mobile adhesin involved in gliding motility.
This document summarizes a thesis project investigating the regulation of the anaerobic responsive transcription factor TdcA in Salmonella Typhimurium. The project aims to construct Salmonella strains with deletions or tags of genes involved in regulating tdcA expression, and to analyze the effects on tdcA transcription and protein binding to its promoter region. Specifically, the project will create ∆tdcA and tdcA-FLAG strains to identify members of the TdcA regulon, and use transcriptional analysis and chromatin immunoprecipitation to study how proteins like CRP, FNR, and H-NS regulate tdcA expression in Salmonella. Understanding TdcA regulation is important because it plays a role in Salmon
The document summarizes the Signal Transducer and Activator of Transcription (STAT) protein family, with a focus on STAT3 and its role in psoriasis. It describes how STAT proteins are activated downstream of cytokine and growth factor receptors via phosphorylation by Janus kinases (JAKs). STAT3 in particular is activated by cytokines like IL-6 and plays roles in processes like acute phase response, cell growth, and embryonic development. Aberrant STAT3 activation has been implicated in conditions like psoriasis and cancer.
This study investigated the functional redundancy of the four aspartic proteinases (plasmepsins) found in the digestive vacuole of the malaria parasite Plasmodium falciparum. The researchers disrupted each of the plasmepsin genes (PfPM1, PfPM2, PfPM4, and PfHAP) through genetic engineering. They found that while disruption of PfPM1 and PfPM4 resulted in reduced parasite growth, none of the plasmepsins were essential for survival. This suggests the plasmepsins can compensate for each other, likely due to their structural and catalytic similarities. The study implies that effective antimalarial drugs will need to inhibit multiple plasmepsin family members.
1) The study identified SIRT2, a histone deacetylase, as a modulator of response to targeted cancer therapies through a genetic screen of epigenetic regulators.
2) Knockdown of SIRT2 conferred resistance to EGFR inhibitors in colon and lung cancer cell lines and to BRAF and MEK inhibitors in melanoma and colon cancer cell lines.
3) Loss of SIRT2 led to increased levels of phosphorylated ERK, suggesting it modulates resistance by regulating MEK kinase activity in the RAS-RAF-MEK-ERK signaling pathway.
The researchers engineered a genetic toolset called pB-Tet-GOI for flexible control of transgene expression in stem and progenitor-derived cell lineages. The system incorporates the latest tetracycline transactivator and reverse transactivator variants to provide regulated transgene expression upon addition or removal of doxycycline. It allows for doxycycline-induced, doxycycline-suppressed, doxycycline-resistant (constitutive), and doxycycline-induced/constitutive regulation of transgenes. Initial tests showed the system provides inducible transgene expression with minimal leakiness and can be used to bidirectionally express reporters and genes of interest to direct cell differentiation.
The document discusses research into understanding how glycosyltransferase enzymes control glycosidic linkage formation during polysaccharide biosynthesis. Specifically, it focuses on GlfT2 enzymes from Mycobacterium tuberculosis, Nocardia brasiliensis, and Rhodococcus equi which produce galactans with different linkage patterns. The research group seeks to gain mechanistic insights by examining differences in the active sites of these GlfT2 orthologs, particularly two 'DXD' motifs known to be critical for M. tb GlfT2 catalysis, through site-directed mutagenesis experiments. They hope this will illuminate how variations in glycosyltransferases guide specific linkage formation.
This document summarizes a presentation on using aptamers to deliver siRNA for gene silencing. The presentation introduces siRNA and RNAi, and discusses the challenge of delivering siRNA to specific cells and tissues. It then describes a study that conjugated an anti-prostate specific membrane antigen (PSMA) aptamer to siRNA targeting GADPH or lamin A/C mRNA. This conjugate selectively delivered functional siRNA to PSMA-positive prostate cancer cells but not PSMA-negative cells. The aptamer-siRNA conjugate showed rapid internalization and gene silencing comparable to commercial reagents. This approach could provide a cell-specific siRNA delivery method without immunogenicity issues of other systems.
This study tested new and modified peptide aptamers to determine their effectiveness in reducing infection by the Cabbage leaf curl virus (CaLCuV) in Arabidopsis plants. The researchers designed primers with targeted peptide sequences, amplified and cloned them onto a geminivirus vector, then inoculated Arabidopsis plants using a gene gun. They found that aptamers A1825 and A1843 showed the best results in reducing infection rates compared to the control, though A1841 showed higher infection but no difference in plant heights. Future work will test additional aptamers and further analyze the impact of peptide aptamers on infection rates.
Genome-wide characterization of AP2/ERF and HSP 90 gene family in select legumesICRISAT
This study characterized transcription factor (AP2/ERF) and molecular chaperone (HSP 90) genes in five legumes: chickpea, pigeonpea, common bean, medicago, and lotus. It identified 147-179 AP2/ERF genes across the five legumes, which were classified into ERF, DREB, AP2, and other subgroups. Fewer HSP 90 genes were identified, ranging from 5-7 per species. Gene structures, phylogenies, and expression under stress provided insights into the roles and evolution of these gene families in legumes.
1. Depletion of the histone acetyltransferase KAT2A reduces the colony-forming potential of normal hematopoietic stem cells, but does not alter their lineage bias. This reinforces other data showing KAT2A depletion does not cause a global downregulation of lineage-specific genes.
2. KAT2A depletion may accelerate stem cell differentiation, leading to an apparent reduction in proliferation. This is supported by an association between "transient clones" and KAT2A knockdown.
3. Further single-cell studies are proposed to better understand how KAT2A impacts cell fate transitions and heterogeneity between sister cells, including analyzing lineage marker expression and first division kinetics through daughter cell
This document provides a curriculum vitae for Devanand Kumar that summarizes his education and work experience. He received a PhD in Microbiology from the University of Delhi, where he identified and characterized histone acetyltransferases in Leishmania donovani. As a post-doctoral researcher, he further characterized the histone acetyltransferase HAT3 in L. donovani and found it plays a role in histone deposition, DNA damage response, and mediates PCNA acetylation and degradation after UV exposure. He has over 10 years of experience in molecular and cellular biology techniques and currently works as a research scientist at Premas Biotech Pvt. Ltd.
The document summarizes a study that found:
1) Interleukin-12 (IL-12) in dendritic cells traffics through late endocytic vesicles marked by the SNARE protein VAMP7.
2) Dendritic cells from VAMP7 knockout mice show partially impaired secretion of bioactive IL-12/p70 dimer.
3) At the immune synapse between dendritic cells and T cells, IL-12 containing vesicles rapidly redistribute and their release is entirely dependent on VAMP7, leading to reduced T cell acquisition of effector functions when stimulated with VAMP7 knockout dendritic cells.
This document summarizes a comparative genome analysis that identified five distinct subfamilies of sortase enzymes in gram-positive bacteria. Sortases are enzymes that anchor surface proteins to the cell wall by recognizing a cell wall sorting signal on the proteins. The analysis examined 72 bacterial genomes and identified 176 sortase homologs that clustered into six subfamilies based on sequence similarity, including two previously known subfamilies (SrtA and SrtB) and three new subfamilies numbered 3, 4, and 5. A hidden Markov model was used to further characterize the subfamilies. The analysis aims to predict which specific sortase enzymes are responsible for anchoring which surface proteins based on the sorting signal motifs.
The complement system is a series of more than 30 proteins that interact in a precise order to enhance host defense. It can be activated through three pathways: the classical pathway involves antibody binding and nine proteins; the alternative pathway is antibody-independent; and the lectin pathway involves mannose-binding lectin binding to pathogens. Complement activation results in cytolysis of target cells, opsonization to enhance phagocytosis, chemotaxis to recruit immune cells, and anaphylaxis.
ShRNA-specific regulation of FMNL2 expression in P19 cellsYousefLayyous
This video encompasses all the steps and data produced for my graduation project in BSc in Biopharmaceutical science. During the course of the project we modified mammalian cells using Short Hairpin RNA to inhibit the correct function of the cytoskelleton. In this way we studied the importance of FMNL2 for the activation and regulation of actin fibers. Among the methods used are Flourescent microscopy, mamallian cell culture, cloning and flow cytometry.
This research article investigates how plant ribosome-inactivating proteins (RIPs) induce cellular stress responses in human cancer cells. The researchers found that two human cancer cell lines exposed to three RIPs - ricin, riproximin and volkensin - activated the unfolded protein response (UPR), a stress response pathway in the endoplasmic reticulum. This suggests the UPR induction better explains the cellular effects of RIPs, as apoptosis was induced even when some protein translation was still occurring due to ribosomal damage. The study provides new insights into the molecular mechanisms by which RIPs exert their toxic effects on cells.
The document discusses hypoxia-inducible factor 1 (HIF-1), which promotes tumor growth through various target genes involved in processes like angiogenesis and drug resistance. The HIF-1α subunit is regulated by oxygen levels and targeted for degradation, making it an important target for cancer therapy. The authors designed G-rich oligonucleotides (ODNs) that form G-quartet structures to selectively target and inhibit HIF-1α. Two lead compounds, JG243 and JG244, decreased HIF-1α and HIF-2α levels and inhibited related proteins without affecting other factors. These JG-ODNs induced degradation of HIF-1α and HIF-2α in
Vitamin C inhibits FAS-induced apoptosis in monocytes and U937 cells by reducing caspase activity and ROS levels. The study found that loading cells with high intracellular concentrations of vitamin C through DHA administration blocked FAS-mediated apoptosis. Vitamin C's primary effect was inhibiting caspase-8 activation, with a separate impact on preserving mitochondrial membrane integrity and reducing ROS. This illuminates vitamin C's role in modulating redox signaling in FAS-induced apoptosis of immune cells.
This document summarizes a study investigating the role of a Plasmodium falciparum S33 proline aminopeptidase (PfPAP) in changes to host red blood cell deformability. The key findings are:
1) PfPAP contains a predicted protein export element suggesting it is exported into infected red blood cells. In silico modeling and recombinant protein studies confirmed PfPAP is a proline aminopeptidase.
2) Genetic deletion of PfPAP in P. falciparum led to an increase in the deformability of infected red blood cells and reduced adherence of infected cells to the endothelial cell receptor CD36 under flow conditions.
3) These results suggest PfP
J. biol. chem. 2016-shao-jbc.m116.724401andrei andrei
FBXO3 promotes ubiquitylation and transcriptional activity of AIRE. The study found that the E3 ubiquitin ligase FBXO3 interacts with phosphorylated residues on AIRE and promotes its ubiquitylation. This post-translational modification increases the interaction between AIRE and P-TEFb, potentiating their transcriptional activity on tissue-specific antigen genes in the thymus. Knockdown of FBXO3 decreased ubiquitylation and transcriptional activity of AIRE.
Bacteria Induced Cryptic Meroterpenoid Pathway in Pathogenic Aspergillus fumi...Debanjan Chatterjee
The document summarizes a presentation on inducing a cryptic meroterpenoid pathway in the pathogenic fungus Aspergillus fumigatus through co-cultivation with the actinomycete Streptomyces rapamycinicus. Co-cultivation led to the activation of a previously silent polyketide synthase gene cluster and the production of novel prenylated polyketides, including Fumicyclines A. Deletion of the polyketide synthase gene confirmed its involvement in biosynthesis. While co-cultivation induced pathway expression, inhibition of histone acetyltransferase did not, suggesting the bacterium alters fungal epigenetic regulation. Understanding secondary metabolism in A. f
1) The document describes a study identifying new genes involved in gliding motility in Flavobacterium johnsoniae.
2) Transposon mutagenesis of an F. johnsoniae sprB mutant identified 8 mutants with increased phage resistance and reduced motility. 4 mutants had transposon insertions in remA, which encodes a cell surface protein with a lectin domain.
3) RemA was shown to localize to the cell surface and move rapidly along the cell surface, suggesting it acts as a mobile adhesin involved in gliding motility.
This document summarizes a thesis project investigating the regulation of the anaerobic responsive transcription factor TdcA in Salmonella Typhimurium. The project aims to construct Salmonella strains with deletions or tags of genes involved in regulating tdcA expression, and to analyze the effects on tdcA transcription and protein binding to its promoter region. Specifically, the project will create ∆tdcA and tdcA-FLAG strains to identify members of the TdcA regulon, and use transcriptional analysis and chromatin immunoprecipitation to study how proteins like CRP, FNR, and H-NS regulate tdcA expression in Salmonella. Understanding TdcA regulation is important because it plays a role in Salmon
The document summarizes the Signal Transducer and Activator of Transcription (STAT) protein family, with a focus on STAT3 and its role in psoriasis. It describes how STAT proteins are activated downstream of cytokine and growth factor receptors via phosphorylation by Janus kinases (JAKs). STAT3 in particular is activated by cytokines like IL-6 and plays roles in processes like acute phase response, cell growth, and embryonic development. Aberrant STAT3 activation has been implicated in conditions like psoriasis and cancer.
This study investigated the functional redundancy of the four aspartic proteinases (plasmepsins) found in the digestive vacuole of the malaria parasite Plasmodium falciparum. The researchers disrupted each of the plasmepsin genes (PfPM1, PfPM2, PfPM4, and PfHAP) through genetic engineering. They found that while disruption of PfPM1 and PfPM4 resulted in reduced parasite growth, none of the plasmepsins were essential for survival. This suggests the plasmepsins can compensate for each other, likely due to their structural and catalytic similarities. The study implies that effective antimalarial drugs will need to inhibit multiple plasmepsin family members.
1) The study identified SIRT2, a histone deacetylase, as a modulator of response to targeted cancer therapies through a genetic screen of epigenetic regulators.
2) Knockdown of SIRT2 conferred resistance to EGFR inhibitors in colon and lung cancer cell lines and to BRAF and MEK inhibitors in melanoma and colon cancer cell lines.
3) Loss of SIRT2 led to increased levels of phosphorylated ERK, suggesting it modulates resistance by regulating MEK kinase activity in the RAS-RAF-MEK-ERK signaling pathway.
The researchers engineered a genetic toolset called pB-Tet-GOI for flexible control of transgene expression in stem and progenitor-derived cell lineages. The system incorporates the latest tetracycline transactivator and reverse transactivator variants to provide regulated transgene expression upon addition or removal of doxycycline. It allows for doxycycline-induced, doxycycline-suppressed, doxycycline-resistant (constitutive), and doxycycline-induced/constitutive regulation of transgenes. Initial tests showed the system provides inducible transgene expression with minimal leakiness and can be used to bidirectionally express reporters and genes of interest to direct cell differentiation.
The document discusses research into understanding how glycosyltransferase enzymes control glycosidic linkage formation during polysaccharide biosynthesis. Specifically, it focuses on GlfT2 enzymes from Mycobacterium tuberculosis, Nocardia brasiliensis, and Rhodococcus equi which produce galactans with different linkage patterns. The research group seeks to gain mechanistic insights by examining differences in the active sites of these GlfT2 orthologs, particularly two 'DXD' motifs known to be critical for M. tb GlfT2 catalysis, through site-directed mutagenesis experiments. They hope this will illuminate how variations in glycosyltransferases guide specific linkage formation.
This document summarizes a presentation on using aptamers to deliver siRNA for gene silencing. The presentation introduces siRNA and RNAi, and discusses the challenge of delivering siRNA to specific cells and tissues. It then describes a study that conjugated an anti-prostate specific membrane antigen (PSMA) aptamer to siRNA targeting GADPH or lamin A/C mRNA. This conjugate selectively delivered functional siRNA to PSMA-positive prostate cancer cells but not PSMA-negative cells. The aptamer-siRNA conjugate showed rapid internalization and gene silencing comparable to commercial reagents. This approach could provide a cell-specific siRNA delivery method without immunogenicity issues of other systems.
This study tested new and modified peptide aptamers to determine their effectiveness in reducing infection by the Cabbage leaf curl virus (CaLCuV) in Arabidopsis plants. The researchers designed primers with targeted peptide sequences, amplified and cloned them onto a geminivirus vector, then inoculated Arabidopsis plants using a gene gun. They found that aptamers A1825 and A1843 showed the best results in reducing infection rates compared to the control, though A1841 showed higher infection but no difference in plant heights. Future work will test additional aptamers and further analyze the impact of peptide aptamers on infection rates.
The document discusses aptamers, which are single-stranded folded oligonucleotides or peptides that bind to molecular targets with high affinity and specificity. Aptamers are produced through an in vitro selection process called SELEX that identifies nucleic acid sequences that bind to a target. The document outlines the SELEX process and compares properties of aptamers to antibodies. Potential applications of aptamers discussed include use as therapeutics, drug delivery agents, diagnostic tools, and in bioimaging and Western blot analysis due to their high specificity and low immunogenicity.
This document summarizes a study on developing a novel protein interaction platform to study neurodegeneration. The study cultured neuronal stem cells to form neurospheres, which were used as a model system. Lentiviral transfection was optimized to introduce target genes stably into the neurospheres. The goal was to analyze protein interactions of Alzheimer's disease proteins by co-immunoprecipitation of neurosphere cultures expressing tagged target proteins. This model aims to further the understanding of molecular mechanisms in neurodegenerative disorders like Alzheimer's disease.
International Journal of Plant Biotechnology
is a comprehensive journal that covers all the topics of plant biotechnology, including plant physiology to plant tissue culture from manipulation of plants to production of secondary metabolites under abiotic and biotic stress. Journal publishes recent advancement in the form of original research articles and review papers.
The document discusses cell culture, which involves growing cells outside their natural environment under controlled conditions. It provides a history of key developments in cell culture, including the first successful culture by Ross Harrison in 1907. It describes primary, secondary, and continuous cell lines. Various types of cell cultures are discussed, including adherent and suspension cultures. Applications of cell culture like model systems, toxicity testing, and cancer research are mentioned. Requirements for cell culture facilities and techniques for maintaining sterile conditions are outlined. Caco-2 cell lines are described as a model for drug absorption studies. Challenges with Caco-2 cell lines are also noted.
Cell lines are permanently established cell cultures that can proliferate indefinitely. They are derived from primary cell cultures isolated from animal or plant tissues. Cell lines may be normal or transformed and can have finite or continuous growth. Different cell lines have various applications including screening drugs, studying cell functions, and producing vaccines and therapeutic proteins. Selecting the appropriate cell line depends on factors like species, growth characteristics, and intended experimental purpose.
Production and purification of Viral vectors for gene and cell therapy appli...Dr. Priyabrata Pattnaik
The cell and gene therapy market is growing rapidly and is projected to reach $10 billion in 5 years. There are three main segments: gene therapy, stem cell therapy, and cell immunotherapy. Gene therapy uses viral vectors like lentivirus or adenovirus to deliver nucleic acids. The production of viral vectors like AAV involves growing HEK 293 cells in bioreactors, transfecting them with plasmids, harvesting and purifying the virus through clarification, filtration, and chromatography. CAR-T cell therapy is also discussed as an example of cell immunotherapy, which uses lentivirus to modify patient T-cells that are then reintroduced to the patient.
Recombinant viral vectors are genetic engineering tools commonly used for gene transfer purpose with high transfection efficiency and site specific gene insertion.
The document discusses olive leaf extract and its health benefits. It notes that olive leaf extract contains powerful antioxidants, has anti-aging properties, supports immune and cardiovascular health, and fights free radicals. The extract contains over 500% more antioxidant capacity than vitamin C. It is suggested for supporting overall wellness and health.
Cell culture involves growing cells from tissue or organ samples in artificial environments outside of the original organism. There are several stages of cell culture, beginning with isolating tissues through enzymatic or mechanical means. Primary cell cultures have a limited lifespan, while continuous cell lines can proliferate indefinitely. Proper culture conditions require appropriate media, substrates, gases, and temperature/humidity control. Cells may be grown as adherent monolayers or in suspension. Cell culture has many applications including drug development, cancer research, and production of therapeutic products.
Viral vectors are efficient tools for gene delivery due to viruses' ability to transfer DNA into host cells. The document discusses several types of viral vectors, including adenoviral, adeno-associated, retroviral, lentiviral, and baculovirus vectors. It provides details on the structure and genome organization of different viruses used to create these vectors. The document also explains the process of generating recombinant viral vectors by removing unnecessary viral genes and inserting genes of interest. Viral vectors allow for transient or stable gene expression and are useful for both research and clinical applications such as gene therapy and vaccine development.
This document summarizes a study investigating the roles of Rho and ADP-ribosylation Factor (ARF) GTPases in regulating phospholipase D (PLD) activity in human lung adenocarcinoma cells. The study used recombinant Sindbis viruses to express Clostridium botulinum C3 exoenzyme (which inactivates Rho) and a dominant-negative Rho mutant in the cells. Expression of C3 or the mutant Rho increased basal PLD activity and decreased phorbol ester-stimulated PLD activity. Bradykinin- or sphingosine-stimulated PLD activity, which had additive effects, was abolished by C3 or mutant Rho expression. Brefeld
1) The study examined how the HIV-1 Tat protein interacts with lipid rafts (LRs) in podocytes isolated from children with HIV-associated nephropathy (HIVAN) and regulates fibroblast growth factor-2 (FGF-2) signaling.
2) It found that Tat preferentially localizes to LRs in podocytes from HIVAN patients. The basic domain of Tat (RKKRRQRRR) was essential for targeting Tat to LRs and enhancing FGF-2 signaling.
3) Mutation of the basic domain (to AKKAAQAAA) prevented Tat from associating with LRs and enhancing FGF-2 signaling. This identifies the key domain of Tat responsible
The B30. 2 domain of pyrin, the familial mediterranean fever protein, interac...José Luis Moreno Garvayo
The document reports on research demonstrating a direct interaction between the B30.2 domain of the pyrin protein and caspase-1. This interaction is independent of the adaptor protein ASC. The B30.2 domain is both necessary and sufficient for binding to caspase-1. Mutations in the B30.2 domain associated with Familial Mediterranean Fever (FMF) reduce this interaction. The pyrin-caspase-1 interaction attenuates IL-1β production, and this inhibitory effect is diminished by FMF-associated B30.2 mutations. This provides a molecular explanation for how mutations in the B30.2 domain could increase inflammation in FMF patients.
The Ras pathway allows cells to respond to external signals by controlling processes like proliferation, survival, and apoptosis. When growth factors bind to receptor tyrosine kinases, it activates Ras which can then activate the MAPK, PI3K, and other pathways to regulate gene expression and cell behavior. Mutations that cause Ras to be constantly active are implicated in many cancers. Inhibiting Ras function through drugs like farnesyltransferase inhibitors may block its ability to drive uncontrolled cell growth.
Screening of receptor like kinase mutants of Arabidopsis thaliana using prote...Thomas Welch
This study screened 17 T-DNA knockout lines of receptor-like kinases in Arabidopsis thaliana for their response to protein extracts of the downy mildew pathogen Hyaloperonospora arabidopsidis using an oxidative burst assay. The assay found that knockout lines of ERL1 and BKK1 had significantly lower oxidative burst responses compared to the wild type, suggesting these genes may be involved in pathogen-associated molecular pattern triggered immunity against H. arabidopsidis. The results provide further evidence for the role of BKK1 in immunity and uncover the potential role of ERL1 in perceiving an obligate biotrophic pathogen, in contrast to previous research focusing on its response to nec
1. The authors aimed to determine if the Arabidopsis thaliana p80 protein localizes to endosomal vesicles like its animal homolog by expressing a GFP fusion of the plant protein in HeLa cells.
2. Control fusion proteins expressed well but the plant p80 protein did not reach sufficient levels for localization analysis, only showing background fluorescence.
3. Low transfection efficiency of the plant protein into human cells was a challenge, suggesting a plant-based system would be better for future studies of p80 protein localization.
This study investigated how oxidative stress activates the PI3K pathway in neurons affected by neurodegenerative diseases. The researchers found that ingestion of the oxidative stress inducer Paraquat in Drosophila larvae caused axonal transport defects and neuronal cell death. Expressing active PI3K suppressed Paraquat-mediated cell death but not axonal blocks, indicating PI3K acts downstream of transport defects. Expression of active PI3K also suppressed cell death from polyQ protein expression but did not affect associated transport defects. Dominant negative PI3K disrupted normal transport of huntingtin protein, linking PI3K directly to transport. Together, the findings suggest axonal transport defects activate the PI3K pathway to decrease oxidative stress-induced
Senior Thesis-Analyzing the interactions between MYOGEF and a component of er...Dougan McGrath
This document summarizes a study analyzing the interaction between MYOGEF, a guanine nucleotide exchange factor that activates RhoA, and SPTA1, a major component of the erythrocyte cytoskeleton. Previous research identified SPTA1 as an interacting partner of MYOGEF. The current study aims to characterize this interaction through constructing cDNA fragments of different regions of MYOGEF and SPTA1 and examining their interaction using yeast two-hybrid and in vitro pull-down assays. The results showed that the C-terminal region of MYOGEF interacted with the EF-hand motifs located in the C-terminal region of SPTA1. This interaction may lead to MYOGEF-mediated
This document summarizes research into the connection between the NF-κB and AP-1 transcription factor pathways. The researchers found that NF-κB regulates the expression of the elk-1 gene, which encodes a protein that activates the transcription of c-fos in response to stimuli. Inhibition of NF-κB activity decreased elk-1 expression and impaired the induction of c-fos and VEGF expression in response to various stimuli. Thus, NF-κB plays an essential role in regulating elk-1, c-fos, and VEGF expression by controlling elk-1 at the transcriptional level.
This document summarizes a study examining the connection between the NF-κB and AP-1 transcription factor pathways. The researchers found that NF-κB regulates the expression of elk-1, a member of the ternary complex factors (TCFs) that activate c-fos expression. Inhibition of NF-κB reduced elk-1 expression, impairing c-fos induction in response to various stimuli. Thus, NF-κB plays an essential role in regulating elk-1, c-fos, and downstream VEGF expression by controlling elk-1 at the transcriptional level. This reveals an interaction between the NF-κB and AP-1 pathways where NF-κB modulates AP-1 activity through regulating one of
Retinoblastoma family proteins New players in DNA repair by non-homologousMaciej Luczynski
This document summarizes recent findings that retinoblastoma family proteins (RB1, p107, p130) play a novel role in regulating DNA repair through non-homologous end-joining (NHEJ). The authors found that RB1's N-terminal domain directly interacts with proteins involved in NHEJ, including XRCC5 and XRCC6. Mutation analysis showed the interaction is mediated by a cyclin wedge surface in the N-terminal domain. Loss of RB1 impaired NHEJ and increased chromosomal abnormalities after irradiation, demonstrating its functional role in DNA repair. The authors hypothesize RB1 facilitates NHEJ by recruiting chromatin modifiers to sites of DNA damage via interactions between its three
Thant Bio Symposium Poster Spring 2016Claire Thant
The study found that axonal transport defects activate the PI3K pathway in an attempt to reduce oxidative stress and neuronal cell death in neurodegenerative disease models. Expressing constitutively active PI3K decreased cell death caused by polyQ repeats and Paraquat exposure but did not affect axonal transport blockages. Dominant negative PI3K disrupted normal huntingtin motility, indicating PI3K acts downstream of transport. Motor protein mutations and disease models showed increased levels of p-GSK3β, a PI3K effector, suggesting transport defects trigger the protective PI3K response.
This master's dissertation aimed to demonstrate gene expression in Rat1 fibroblast cells transformed by EVI1 and the relationship between EVI1 levels and CAIII gene expression. Real-time PCR and western blotting showed higher CAIII gene and protein expression in Rat1neo cells compared to Rat15.6 cells, which overexpress EVI1. Luciferase assays also demonstrated higher activity in Rat1neo cells, indicating higher CAIII expression. Silencing CAIII in Rat1neo cells increased caspase 3 activity after hydrogen peroxide treatment, showing CAIII protects against apoptosis. The results suggest EVI1 overexpression represses CAIII expression, reducing protection against oxidative stress. Therefore, oxidative stress agents may selectively target cancer cells overexpressing
RTEL1 is a replisome-associated helicase that interacts with proliferating cell nuclear antigen (PCNA) through a PCNA interaction protein motif (PIP box). The study found that disrupting the RTEL1-PCNA interaction in mice (RTEL1 PIP mutant) led to replication fork instability, reduced fork extension rates, increased origin usage, and accelerated senescence. While T-loop disassembly at telomeres was unaffected, telomere replication was compromised, causing fragile telomeres. The RTEL1 PIP mutant mice were viable but loss of the RTEL1-PCNA interaction accelerated tumorigenesis in p53-deficient mice, suggesting an important role for
This study examines the correlation between cytoplasmic p21 levels, AKT activation, and response to tamoxifen in breast cancer patients. The results show that cytoplasmic p21 levels strongly correlate with pAKT levels and predict a poor response to tamoxifen therapy. In vitro experiments demonstrate that the growth factor HRG β 1 induces both nuclear and cytoplasmic p21 expression in breast cancer cells, impairing the inhibitory effects of tamoxifen on cell cycle progression and apoptosis.
2011 - Cellular inhibitor of apoptosis protein-1 (cIAP1) can regulate E2F1 tr...Simon Gemble
Cellular inhibitor of apoptosis protein-1 (cIAP1) can directly interact with the transcription factor E2F1 and increase its transcriptional activity. cIAP1 is recruited to E2F1 binding sites on cyclin E and cyclin A promoters in a cell cycle-dependent manner. Silencing cIAP1 inhibits E2F1 DNA binding and transcriptional activation of cyclin E, reducing cell proliferation. Thus, one function of nuclear cIAP1 is to regulate E2F1 transcriptional activity and control cell cycle progression.
The document describes a study investigating the molecular mechanisms by which an extract from the brown seaweed Fucus vesiculosus (Fv1) mediates cell cycle inhibition and cell death in pancreatic cancer cells. Gene expression profiling found that Fv1 treatment upregulated the cell cycle inhibitor p57 and downregulated genes involved in cell cycle progression. This suggests Fv1 induces cell cycle arrest. Fv1 also altered the morphology of treated cells and inhibited their viability and proliferation in a caspase-independent manner. Proliferation was found to be a prerequisite for Fv1's effectiveness.
Environmental Factor - July 2014_ Intramural papers of the monthXunhai 郑训海
The document summarizes 5 research papers from the National Institute of Environmental Health Sciences (NIEHS). It describes the key findings and conclusions from each paper which include: 1) NIEHS developed a 7-step framework for systematic reviews to address environmental health questions. 2) A study found that polymerase beta can complement aprataxin function during DNA repair. 3) Retinoic acid-related orphan receptor gamma regulates hepatic glucose metabolism and insulin sensitivity. 4) The INO80 complex maintains embryonic stem cell pluripotency and regulates blastocyst development. 5) A study characterized structural changes in HIV reverse transcriptase formation providing insights for new therapeutics.
Oncogenic Ras promotes the survival of cancer cells detached from the extracellular matrix (ECM) through distinct downstream pathways. Ras activates phosphatidylinositol 3-kinase (PI3K) signaling to regulate metabolism in detached cells, but surprisingly does not do so through Akt. Instead, Ras utilizes serum and glucocorticoid-regulated kinase 1 (SGK1) to promote ATP generation. Additionally, Ras blocks anoikis or detachment-induced apoptosis by reducing expression of the phosphatase PHLPP1, which activates p38 MAPK to induce anoikis. Thus, Ras facilitates survival of detached cancer cells through divergent effectors to regulate metabolism and anoikis.
2016 - A balanced pyrimidine pool is required for optimal Chk1 activation to ...Simon Gemble
This document summarizes a study investigating how a decrease in poly(ADP-ribose) polymerase 1 (PARP-1) activity in cells deficient for cytidine deaminase (CDA) leads to impaired activation of checkpoint kinase 1 (Chk1) and less efficient DNA damage checkpoints. The study found that CDA deficiency results in lower levels of Chk1 bound to chromatin and reduced phosphorylation/activation of Chk1 in response to genotoxic stress. This compromised Chk1 activation leads to less efficient S-phase and G2-M checkpoints and accumulation of unreplicated DNA during mitosis, resulting in ultrafine anaphase bridge formation. The results reveal an unexpected link between nucleotide pool balance
Similar to E-cadherin-based complexes regulate cell behaviour (20)
29 сентября состоятся Общественные слушания по вопросам реформирования здраво...oncoportal.net
От украинской онкологии в Общественных слушаниях участие примет Олег Спиженко, советник министра кабинета министров Украины по вопросам здравоохранения, Глава ассоциации частной медицины Украины, Генеральный директор Медицинского центра “Клиника Спиженко”.
EARLY CANCER DIAGNOSIS - guide from World Health Organization, 2017oncoportal.net
GUIDE TO CANCER EARLY DIAGNOSIS - World Health Organization 2017
Руководство по РАННЕЙ ДИАГНОСТИКЕ РАКА, Всемирная организация здравоохранения, 2017 год, 48 страниц, на английском языке.
Журнал "Медицинские аспекты здоровья женщины". Тематический выпуск "Онкогинекология"
№ 5 (91) 2015
Издательский дом «Здоровье Украины. Медицинские издания»
Приглашение на конференцию "Современные подходы к диагностике и лечению лимфо...oncoportal.net
«Современные подходы к диагностике и лечению лимфопролиферативных заболеваний» ‒ VII научно-практическая конференция с международным участием, Киев, 3-4 ноября 2016
программа прекурса СОВРЕМЕННЫЕ МЕТОДЫ ДИАГНОСТИКИ И ТЕРАПИИ В ДЕРМАТООНКОЛОГИИoncoportal.net
11-Е КИЕВСКИЕ ДЕРМАТОЛОГИЧЕСКИЕ ДНИ – КОНФЕРЕНЦИЯ, 12-14 ОКТЯБРЯ
12-14 октября 2016 года в столице Украины состоится ежегодное профессиональное событие в сфере специалистов-дерматологов ‒ конференция «11-е Киевские дерматологические дни». Организатором конференции выступает общественная организация «Украинская академия дерматовенерологии» (УАДВ).
Мероприятие предусматривает проведение 3-х тематических прекурсов с освещением уникальных тематик с привлечением лучших спикеров в дерматовенерологии и смежных специальностях. Все три прекурса будут проведены в один день ‒12 октября, один из них, в частности, будет посвящен актуальным вопросам диагностики и лечения новообразований кожи.
• Прекурс №1 «Клеточная терапия в дерматологии и эстетической медицине».
• Прекурс №2 «Менеджмент инфекций передаваемых половым путем и урогенитальных инфекций. Опыт ведущих эспертов в дерматовенерологии, урологии и гинекологии».
• Прекурс №3 «Современные методы диагностики и терапии в дерматоонкологии» ‒ программа прекурса в файле для просмотра и скачивания
Сколько каждого из наименования лекарственных средств для взрослой государственной онкологии Украины куплено и состояние закупки - Выдержка из информации о закупках лекарственных средств в категории "Взрослая онкология", проведенных через международные организации за счет средств Госбюджета на 2015 год по состоянию на 15.08.2016
Сколько каждого из наименования лекарственных средств для детской государственной онкологии Украины куплено и состояние закупки - Выдержка из информации о закупках лекарственных средств в категории "Взрослая онкология", проведенных через международные организации за счет средств Госбюджета на 2015 год по состоянию на 15.08.2016
Сколько каждого из наименования лекарственных средств для детской государственной онкологии Украины куплено и состояние закупки - Выдержка из информации о закупках лекарственных средств в категории "Детская онкология", проведенных через международные организации за счет средств Госбюджета на 2015 год по состоянию на 15.08.2016
ПРОГРАМА: Науково-практична конференція з міжнародною участю «Актуальні питання радіаційної онкології в Україні».
в м. Ужгород (30 червня – 1 липня 2016)
Перелік лікарських засобів, що вже надійшли та будуть поставлені в Україну наприкінці травня-на початку червня 2016 року, за державною програмою дитяча онкологія, які закуповуються Crown Agents
Перелік лікарських засобів, що вже надійшли та будуть поставлені в Україну наприкінці травня-на початку червня 2016 року, за державною програмою доросла онкологія, які закуповуються Crown Agents
сравнительный анализ методов преинвазивной диагностики меланомы кожи, козлов ...oncoportal.net
Результаты исследования, которое включало:
1) Оценку эффективность диагностики меланомы и других видов рака кожи с исполь-
зованием радиотермометрии, флуоресцентной диагностики, дерматоскопии;
2) Сравнение эффективности диагностики с помощью радиотермометрии, флуоресцентной диагностики, дерматоскопии.
Basavarajeeyam is a Sreshta Sangraha grantha (Compiled book ), written by Neelkanta kotturu Basavaraja Virachita. It contains 25 Prakaranas, First 24 Chapters related to Rogas& 25th to Rasadravyas.
share - Lions, tigers, AI and health misinformation, oh my!.pptxTina Purnat
• Pitfalls and pivots needed to use AI effectively in public health
• Evidence-based strategies to address health misinformation effectively
• Building trust with communities online and offline
• Equipping health professionals to address questions, concerns and health misinformation
• Assessing risk and mitigating harm from adverse health narratives in communities, health workforce and health system
Local Advanced Lung Cancer: Artificial Intelligence, Synergetics, Complex Sys...Oleg Kshivets
Overall life span (LS) was 1671.7±1721.6 days and cumulative 5YS reached 62.4%, 10 years – 50.4%, 20 years – 44.6%. 94 LCP lived more than 5 years without cancer (LS=2958.6±1723.6 days), 22 – more than 10 years (LS=5571±1841.8 days). 67 LCP died because of LC (LS=471.9±344 days). AT significantly improved 5YS (68% vs. 53.7%) (P=0.028 by log-rank test). Cox modeling displayed that 5YS of LCP significantly depended on: N0-N12, T3-4, blood cell circuit, cell ratio factors (ratio between cancer cells-CC and blood cells subpopulations), LC cell dynamics, recalcification time, heparin tolerance, prothrombin index, protein, AT, procedure type (P=0.000-0.031). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and N0-12 (rank=1), thrombocytes/CC (rank=2), segmented neutrophils/CC (3), eosinophils/CC (4), erythrocytes/CC (5), healthy cells/CC (6), lymphocytes/CC (7), stick neutrophils/CC (8), leucocytes/CC (9), monocytes/CC (10). Correct prediction of 5YS was 100% by neural networks computing (error=0.000; area under ROC curve=1.0).
ABDOMINAL TRAUMA in pediatrics part one.drhasanrajab
Abdominal trauma in pediatrics refers to injuries or damage to the abdominal organs in children. It can occur due to various causes such as falls, motor vehicle accidents, sports-related injuries, and physical abuse. Children are more vulnerable to abdominal trauma due to their unique anatomical and physiological characteristics. Signs and symptoms include abdominal pain, tenderness, distension, vomiting, and signs of shock. Diagnosis involves physical examination, imaging studies, and laboratory tests. Management depends on the severity and may involve conservative treatment or surgical intervention. Prevention is crucial in reducing the incidence of abdominal trauma in children.
Rasamanikya is a excellent preparation in the field of Rasashastra, it is used in various Kushtha Roga, Shwasa, Vicharchika, Bhagandara, Vatarakta, and Phiranga Roga. In this article Preparation& Comparative analytical profile for both Formulationon i.e Rasamanikya prepared by Kushmanda swarasa & Churnodhaka Shodita Haratala. The study aims to provide insights into the comparative efficacy and analytical aspects of these formulations for enhanced therapeutic outcomes.
8 Surprising Reasons To Meditate 40 Minutes A Day That Can Change Your Life.pptxHolistified Wellness
We’re talking about Vedic Meditation, a form of meditation that has been around for at least 5,000 years. Back then, the people who lived in the Indus Valley, now known as India and Pakistan, practised meditation as a fundamental part of daily life. This knowledge that has given us yoga and Ayurveda, was known as Veda, hence the name Vedic. And though there are some written records, the practice has been passed down verbally from generation to generation.
Integrating Ayurveda into Parkinson’s Management: A Holistic ApproachAyurveda ForAll
Explore the benefits of combining Ayurveda with conventional Parkinson's treatments. Learn how a holistic approach can manage symptoms, enhance well-being, and balance body energies. Discover the steps to safely integrate Ayurvedic practices into your Parkinson’s care plan, including expert guidance on diet, herbal remedies, and lifestyle modifications.
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotesPsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
Osteoporosis - Definition , Evaluation and Management .pdfJim Jacob Roy
Osteoporosis is an increasing cause of morbidity among the elderly.
In this document , a brief outline of osteoporosis is given , including the risk factors of osteoporosis fractures , the indications for testing bone mineral density and the management of osteoporosis
- Video recording of this lecture in English language: https://youtu.be/kqbnxVAZs-0
- Video recording of this lecture in Arabic language: https://youtu.be/SINlygW1Mpc
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