Radioimmunoassay (RIA) is a sensitive technique for measuring trace amounts of substances using the principles of antibody-antigen reactions and radioactive isotopes. It involves a competitive binding reaction between a fixed amount of labeled analyte and a variable amount of unlabeled analyte for a limited number of antibody binding sites. A standard curve is developed to quantify the amount of analyte in unknown samples. RIA can measure analytes like hormones, drugs, and proteins in quantities as small as picograms. It has applications in fields like cancer detection, drug testing, and research on neurotransmitters.