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Production of Feed and Energy from Jatropha curcas
                             • June 11th, 2009
                             • RRB Ghent
                             • Dr. Henk Joos
D1 Oils plc



    Why Jatropha curcas?

        Jatropha curcas is a hardy oilseed bearing tree.
              Centre of Origin in Central America.
              Commonly used in tropical growing areas as a hedge and source of oil.
              Can tolerate drought spells.
        A plant of many revenue opportunities – oil, power generation, animal feed and fertiliser
        Strong global demand for a sustainable energy product, will help countries/ industries meet climate
        treaty requirements.
        Jatropha curcas has high sustainability potential:
              Can be mixed or intercropped alongside existing vegetables/grains, resulting in additional
              and balanced cash income for farmers.
              Potential GHG savings of up to 66%.
        Jatropha curcas remains to be domesticated.



D1 Oils Plant Science – v.May 2009 - 2
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    Global Update Jatropha curcas

        Jatropha curcas has been planted in a number of different productions models in 25+ countries
        around the world
              Currently 500.000 ha + of planting in the ground.
              Majority was planted with “grain’’ and not with ‘’seed’’ of selected varieties.
              The agronomy knowledge base starts to develop but needs refining on a region by region
              basis. This is normal at the stage of domistication of the crop.
        First oil has been produced in different countries from 2-3 year old planting. Volumes expected to
        grow significantly in coming years but still relatively small volumes compared to regular plant
        derived commodity oils.
              Oil has been used primarely locally as a diesel replacement in generators and has been
              used also in biodiesel and shipping/aviation fuel experiments.
              Meal is used locally as biomass or fertilizer.
        A number of projects suffering under current economic crisis and the downturn in investment
        appetite.

D1 Oils Plant Science – v.May 2009 - 3
D1 Oils plc



    Next Steps

        Professional domestication of the crop in areas where it can perform.
              Move Jatropha from being a miracle crop to being a cash crop.
              Start to grow seed of selected varieties in correct adaptation zones.
        Integration of the crop alongside existing cropping expertise.
              Move towards an integrated production of food, feed, fuel and fiber!
        Further professionalization of the Jatropha industry.
              Capitalization of credible projects.
              Jatropha industry Association.
        Value added in the chain by maximal exploitation of oil and by-product streams.
              Animal feed applications
              Aviation
              Other by-product valorization.


D1 Oils Plant Science – v.May 2009 - 4
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    The D1 Oils Science Platform




D1 Oils Plant Science – v.May 2009 - 5   5
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   D1 Oils Science Objectives
                                                                                                             6




                                         Improve oil yield per hectare of Jatropha through agronomy
                                         and breeding.

                                         Select and breed Jatropha cultivars adapted to different
                                         target growing areas.

                                         Optimise oil quality for different end use markets.

                                         Ensure maximised economic value from by-products – e.g.
                                         meal for animal feed, energy from biomass.




D1 Oils Plant Science – v.May 2009 - 6
D1 Oils plc



    Jatropha curcas is not a miracle crop, but....

               Like any crop cultivated to produce grain, Jatropha curcas also needs to be:

                      Cultivated in areas where it can perform.
                      Grown from selected and performing genetics.
                      Grown under good agricultural practise.

               Jatropha can perform in a wide range of environments and needs relatively low water input.

               Jatropha can be developed into a sustainable crop. GHG savings of up to 65%.

               Jatropha is producing a number of anti-nutritional compounds making it unpallatable for
               animals to eat.



D1 Oils Plant Science – v.May 2009 - 7
D1 Oils plc



    Jatropha curcas is not a miracle crop, but....

        Professional Jatropha best practise guides and Jatropha crop calendars have been
        developed on a region by region basis.

        Jatropha should be grown in areas where it can be profitable for farmers and become a cash
        crop. Crop suitability maps have been developed.

        The first selected Jatropha cultivars have been released with a gain in yield of 25% +
              Better seed and seedling quality
              Higher grain yield per ha
              Higher oil content.




D1 Oils Plant Science – v.May 2009 - 8
Selected Cultivars for commercialization in 2010
Cumulative oil yield/Ha and Oil content grain (0-31M)
                                                        D1 Oils plc




                                                                      9




D1 Oils Plant Science – v.May 2009 - 9
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    Jatropha as an energy crop

               Jatropha produces several potential energy sources:

                     Oil (30-40% on grain weight basis).
                           up to 2 tonnes per ha based on current genetics.
                     Jatropha husks and hulls.
                     Jatropha meal after crushing.
                     Jatropha prunings (woodships).

               For every tonne of crude Jatropha Oils there will be:

                     1,25 tonne husks
                     1 tonne hulls
                     1 tonne seedcake


D1 Oils Plant Science – v.May 2009 - 10
D1 Oils plc



    Jatropha curcas Oil


               Jatropha curcas produce an oil fit for

                      Electricity generators
                      Esterification into biodiesel
                      Hydrocracking
                      Aviation and Shipping fuels

               Jatropha oil is fit for local use in tropical and subtropical countries with a high deficit for fossil
               fuel sources and can longer term become significant source of export revenues with surplus
               sales.




D1 Oils Plant Science – v.May 2009 - 11
D1 Oils plc



      Fatty Acid profile Jatropha accessions




D1 Oils Plant Science – v.May 2009 - 12
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    By-product differentiation

                                           Jatropha
                                           Grains



                       Biofuel             Oil                          Biofuel


                       Biomass             Hulls                        Biomass


                       Fertilizer          Kernel                       Animal Feed

                                          Removal of anti nutritional
                                          factors



D1 Oils Plant Science – v.May 2009 - 13                                           13
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    Proxy analysis of high protein meal
                                     D1 Processed        Soy meal             Soy meal
                                       Jatropha     (solvent extraction )   (mechanical
                                                          5-04-604           extraction)
                                                                               5-04-600
                                          67.89%           51.50%             48.70%
      Protein, Crude
                                          6.27%            6.70%               6.70%
      Fiber, Crude
                                          5.31%            1.00%               5.20%
      EE-Fat
                                          9.77%            6.50%               6.30%
      Ash
                                          20.53%           34.30%             33.10%
      NFE-Nitrogen Free Extract
                                          8.05%              6%                 10%
      ADF
                                          11.14%             9%                 15%
      NDF
      TDN-Total Digestible
                                          89.26%           81.00%             85.00%
      Nutrients
                                           3.94             3.57                3.75
      DE-Digestible Energy
                                          97.80%                              94.64%
      IVTDMD

D1 Oils Plant Science – v.May 2009 - 14                                        14
D1 Oils plc



    Amino Acid Analysis of high protein meal

                                             Soybean amino acid data vrs. D1 JSC profile

                      8

                      7

                      6

                      5

                      4                                                                                Soybean
                                                                                                       D1 Profile
                      3

                      2

                      1

                      0
                          Arg   Hist   Iso     Leu   Lys   Meth   Cys   Phen   Tyr   Thr   Try   Val




D1 Oils Plant Science – v.May 2009 - 15                                                                             15
D1 Oils plc



 Protein rich animal feed derived from Jatropha grains

    Activity                              Proof of?             Scale        Milestone Status
                        What?                     How?
    Lab scale           Removal ANF               −Analytical   g amounts    Q2,2008      done
                                                  −Bioassay
    Pilot scale         Removal ANF               −Analytical   kg amounts   Q1,2009      done
                                                  −Bioassay

    Digestability Can animals digest              −Chicken      G amounts    Q3/4,2008    done
    In Vitro      it?                             −Ruminant     Kg amounts   Q1,2009      done
    Palatability        Do animals like it?       −Ruminant     Kg amounts   2010        TBD
    Productivity        Do animals grow on        −Chicken      Kg amounts   2009      TBD
                        it?                       −Ruminant     Ton          2010-2011
                                                                Amounts
D1 Oils Plant Science – v.May 2009 - 16
D1 Oils plc



    Jatropha curcas High Protein Meal


            Based on a proprietary production process D1 Oils extracts from Jatropha curcas grains a
            protein meal with:

                   Effective removal of all anti nutirional compounds
                   High protein content (60%+).
                   High digestible energy content.
                   Excellent amino acid profile protein profile.
                   Excellent in vitro digestibility profiles for ruminants and chickens.

            Scale up and confirmative productivity trials remain to be done to confirm the meal as an
            high protein animal feed component.




D1 Oils Plant Science – v.May 2009 - 17
Thank you for your attention



CONCLUSIONS

 Jatropha is not a miracle crop, but when farmed correctly it can
 deliver its high potential as a sustainable and economical industrial
 crop.

 Jatropha curcas produces a very versatile oil with short term
 production economics better than rapeseed and with longer term
 potential similar to oil palm.

 D1 Oils Plant Science has developed a proprietary process to extract
 high value protein meal from Jatropha grains with an potential added
 value per ha of 1000 USD +.

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Production of Feed and Energy from Jatropha Curcas

  • 1. D1 Oils plc Production of Feed and Energy from Jatropha curcas • June 11th, 2009 • RRB Ghent • Dr. Henk Joos
  • 2. D1 Oils plc Why Jatropha curcas? Jatropha curcas is a hardy oilseed bearing tree. Centre of Origin in Central America. Commonly used in tropical growing areas as a hedge and source of oil. Can tolerate drought spells. A plant of many revenue opportunities – oil, power generation, animal feed and fertiliser Strong global demand for a sustainable energy product, will help countries/ industries meet climate treaty requirements. Jatropha curcas has high sustainability potential: Can be mixed or intercropped alongside existing vegetables/grains, resulting in additional and balanced cash income for farmers. Potential GHG savings of up to 66%. Jatropha curcas remains to be domesticated. D1 Oils Plant Science – v.May 2009 - 2
  • 3. D1 Oils plc Global Update Jatropha curcas Jatropha curcas has been planted in a number of different productions models in 25+ countries around the world Currently 500.000 ha + of planting in the ground. Majority was planted with “grain’’ and not with ‘’seed’’ of selected varieties. The agronomy knowledge base starts to develop but needs refining on a region by region basis. This is normal at the stage of domistication of the crop. First oil has been produced in different countries from 2-3 year old planting. Volumes expected to grow significantly in coming years but still relatively small volumes compared to regular plant derived commodity oils. Oil has been used primarely locally as a diesel replacement in generators and has been used also in biodiesel and shipping/aviation fuel experiments. Meal is used locally as biomass or fertilizer. A number of projects suffering under current economic crisis and the downturn in investment appetite. D1 Oils Plant Science – v.May 2009 - 3
  • 4. D1 Oils plc Next Steps Professional domestication of the crop in areas where it can perform. Move Jatropha from being a miracle crop to being a cash crop. Start to grow seed of selected varieties in correct adaptation zones. Integration of the crop alongside existing cropping expertise. Move towards an integrated production of food, feed, fuel and fiber! Further professionalization of the Jatropha industry. Capitalization of credible projects. Jatropha industry Association. Value added in the chain by maximal exploitation of oil and by-product streams. Animal feed applications Aviation Other by-product valorization. D1 Oils Plant Science – v.May 2009 - 4
  • 5. D1 Oils plc The D1 Oils Science Platform D1 Oils Plant Science – v.May 2009 - 5 5
  • 6. D1 Oils plc D1 Oils Science Objectives 6 Improve oil yield per hectare of Jatropha through agronomy and breeding. Select and breed Jatropha cultivars adapted to different target growing areas. Optimise oil quality for different end use markets. Ensure maximised economic value from by-products – e.g. meal for animal feed, energy from biomass. D1 Oils Plant Science – v.May 2009 - 6
  • 7. D1 Oils plc Jatropha curcas is not a miracle crop, but.... Like any crop cultivated to produce grain, Jatropha curcas also needs to be: Cultivated in areas where it can perform. Grown from selected and performing genetics. Grown under good agricultural practise. Jatropha can perform in a wide range of environments and needs relatively low water input. Jatropha can be developed into a sustainable crop. GHG savings of up to 65%. Jatropha is producing a number of anti-nutritional compounds making it unpallatable for animals to eat. D1 Oils Plant Science – v.May 2009 - 7
  • 8. D1 Oils plc Jatropha curcas is not a miracle crop, but.... Professional Jatropha best practise guides and Jatropha crop calendars have been developed on a region by region basis. Jatropha should be grown in areas where it can be profitable for farmers and become a cash crop. Crop suitability maps have been developed. The first selected Jatropha cultivars have been released with a gain in yield of 25% + Better seed and seedling quality Higher grain yield per ha Higher oil content. D1 Oils Plant Science – v.May 2009 - 8
  • 9. Selected Cultivars for commercialization in 2010 Cumulative oil yield/Ha and Oil content grain (0-31M) D1 Oils plc 9 D1 Oils Plant Science – v.May 2009 - 9
  • 10. D1 Oils plc Jatropha as an energy crop Jatropha produces several potential energy sources: Oil (30-40% on grain weight basis). up to 2 tonnes per ha based on current genetics. Jatropha husks and hulls. Jatropha meal after crushing. Jatropha prunings (woodships). For every tonne of crude Jatropha Oils there will be: 1,25 tonne husks 1 tonne hulls 1 tonne seedcake D1 Oils Plant Science – v.May 2009 - 10
  • 11. D1 Oils plc Jatropha curcas Oil Jatropha curcas produce an oil fit for Electricity generators Esterification into biodiesel Hydrocracking Aviation and Shipping fuels Jatropha oil is fit for local use in tropical and subtropical countries with a high deficit for fossil fuel sources and can longer term become significant source of export revenues with surplus sales. D1 Oils Plant Science – v.May 2009 - 11
  • 12. D1 Oils plc Fatty Acid profile Jatropha accessions D1 Oils Plant Science – v.May 2009 - 12
  • 13. D1 Oils plc By-product differentiation Jatropha Grains Biofuel Oil Biofuel Biomass Hulls Biomass Fertilizer Kernel Animal Feed Removal of anti nutritional factors D1 Oils Plant Science – v.May 2009 - 13 13
  • 14. D1 Oils plc Proxy analysis of high protein meal D1 Processed Soy meal Soy meal Jatropha (solvent extraction ) (mechanical 5-04-604 extraction) 5-04-600 67.89% 51.50% 48.70% Protein, Crude 6.27% 6.70% 6.70% Fiber, Crude 5.31% 1.00% 5.20% EE-Fat 9.77% 6.50% 6.30% Ash 20.53% 34.30% 33.10% NFE-Nitrogen Free Extract 8.05% 6% 10% ADF 11.14% 9% 15% NDF TDN-Total Digestible 89.26% 81.00% 85.00% Nutrients 3.94 3.57 3.75 DE-Digestible Energy 97.80% 94.64% IVTDMD D1 Oils Plant Science – v.May 2009 - 14 14
  • 15. D1 Oils plc Amino Acid Analysis of high protein meal Soybean amino acid data vrs. D1 JSC profile 8 7 6 5 4 Soybean D1 Profile 3 2 1 0 Arg Hist Iso Leu Lys Meth Cys Phen Tyr Thr Try Val D1 Oils Plant Science – v.May 2009 - 15 15
  • 16. D1 Oils plc Protein rich animal feed derived from Jatropha grains Activity Proof of? Scale Milestone Status What? How? Lab scale Removal ANF −Analytical g amounts Q2,2008 done −Bioassay Pilot scale Removal ANF −Analytical kg amounts Q1,2009 done −Bioassay Digestability Can animals digest −Chicken G amounts Q3/4,2008 done In Vitro it? −Ruminant Kg amounts Q1,2009 done Palatability Do animals like it? −Ruminant Kg amounts 2010 TBD Productivity Do animals grow on −Chicken Kg amounts 2009 TBD it? −Ruminant Ton 2010-2011 Amounts D1 Oils Plant Science – v.May 2009 - 16
  • 17. D1 Oils plc Jatropha curcas High Protein Meal Based on a proprietary production process D1 Oils extracts from Jatropha curcas grains a protein meal with: Effective removal of all anti nutirional compounds High protein content (60%+). High digestible energy content. Excellent amino acid profile protein profile. Excellent in vitro digestibility profiles for ruminants and chickens. Scale up and confirmative productivity trials remain to be done to confirm the meal as an high protein animal feed component. D1 Oils Plant Science – v.May 2009 - 17
  • 18. Thank you for your attention CONCLUSIONS Jatropha is not a miracle crop, but when farmed correctly it can deliver its high potential as a sustainable and economical industrial crop. Jatropha curcas produces a very versatile oil with short term production economics better than rapeseed and with longer term potential similar to oil palm. D1 Oils Plant Science has developed a proprietary process to extract high value protein meal from Jatropha grains with an potential added value per ha of 1000 USD +.