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Dr.rubin results presentation group #4
- 1. Tropical Soil Bacteria Isolation& Characterization Tornés-Blanco Anibal Alicea-Pauneto Abneil D. Colón-Rivera Ramón B. González-Piñeiro Ann
- 2.
- 3. Fieldwork Data I Sample Extraction Date& Time Collected Site Features Humidity Temperature pH Sample A - Centro Médico 01/28/15 5:42 pm Urban Moist 302 K 7.33 Sample B- Spout 01/29/15 6:45 am Semi Rural Moist 296 K 6.50
- 4. Fieldwork Data II SampleExtraction Date & Time Collected Site Features Humidity Temperature pH Sample C - Asomante, Aibonito 01/29/15 7:30 a.m. Semi Rural Moist 293 K 5.00 Sample D- Toita, Cayey 01/28/15 5:30 pm Rural Moist 298 K 5.50
- 5. Soil Recollection w/CoordinatesI Sample A - 18°23'45.3"N 66°04'27.0"W Sample B - 18°12'26.9"N 66°03'10.4"W
- 6. Soil Recollection w/CoordinatesII Sample C - 18°8'3"N 66°16'23"W Sample D - 18°14'42.2"N 66°20'20.6"W
- 8. Characterization Results I BacteriaDesignator Code Samples PCR & Electrophoresis Bioinformatic Analysis ATB 30R01A Sample A + N/A ADAP 30R01A Sample B + N/A ADAP 30R01B + N/A
- 9. Characterization Results II BacteriaDesignator Code Samples PCR & Electrophoresis Bioinformatic Analysis RBCR 30P01A Sample C - N/A AMGP 30P01A Sample D - N/A
- 10. R2A Purifications I SampleA Sample B ADAP30R01A ADAP30R01B ATB30R01A
- 11. Purifications II ISP4 (SampleC) LB Medium (Sample D)* f*Experimental Variation*
- 12. Gram Stain I- Gram Positive Sample A Sample B ADAP30R01A ATB30R01A ADAP30R01B
- 13. Gram Stain II- Gram Positive Sample C Sample D RBCR 30P01A AMGP 30P01A
- 14. Morphology I Sample A: -Form: Circular - Elevation: Raised - Margin: Entire Sample B: - Form: Circular - Elevation: Convex - Margin: Entire
- 15. Morphology II Sample C: -Form: Circular - Elevation: Flat - Margin: Curled Sample D: - Form: Circular - Elevation: Convex - Margin: Entire
- 16. Antibiotic Production I SampleA Sample B
- 17. Antibiotic Production II SampleDSample C M. Lutheus (-) E. Coli (-)M. Lutheus (-) E. Coli (-)
- 18. Electrophoresis #1 -Samples A-D Slots 1-12 B C A D Slots 13-24
- 19. ● Howard Hughesand RISE Program at UPR Cayey ● Dr. Michael Rubin ● Mr. Giovanni Cruz ● National Institute of Health
Editor's Notes
- #2 Abneil Good afternoon, we are group #4 We will be talking about tropical soil bacteria Isolation & Characterization You know our names We will have bacteria designator
- #4 Anibal Both samples were moist Sample A being Urban, had a more basic pH than the rural
- #5 Ramon Moist pH did not have a big difference
- #6 Abneil Sample A: Was taken beside a pipeline that comes from the surgery floor Sample B: Is right beside a spout that people from the area believe that could be very contaminated
- #7 Ann Sample C: area that will be used to build an urbanization Sample D: Surrounded by La Plata River
- #8 Abneil This Geographic information system map recorded where each sample was collected. Sample A: was collected San Juan B: was collected in Caguas C: in Aibonito D:here in Cayey
- #9 Anibal Regarding the GIS map we showed previously we have different bacterial designator codes, PCR & Electrophoresis gave positive
- #10 Ramon Our antibiotic production was negative. The PCR and Electrophoresis was negative.
- #11 Abneil Sample A & B were purified in R2A mediums instead of ISP4 because they showed better efficacy
- #12 Ann Sample C and D were purified in ISP4 Fungus grew and the medium had to change to LB
- #13 Anibal Sample A: Gram stain was repeated thrice Sample B: Was repeated twice and we concluded that they were both Gram Positive Homogeneous
- #14 Ramon Sample C: Gram stain from the isolation #3 Sample D: gram stain with the purification #4 Was no Homogeneous
- #15 Abneil The plate interpretations for Sample A & B were similar in form and Margin but different in elevation.
- #16 Ann Both their forms were circular C was flat , Margin Curled D was convex, Margin Entire
- #17 Anibal The reaction of our bacterias to m.Luteus and e.Coli proved negative since they did not inhibit them
- #18 Ramon The bacteria dont inhibit E.coli and M.lutheus
- #19 Ann Sample A(1) bacteria proved positive, Sample B both bacteria proved positive with the initial primers C & D were negative but should be exposed to future work regarding them both.