Application of phage typing and pulsed-field gel electrophoresis to analyse Salmonella enterica isolates from a suspected outbreak in Lagos, Nigeria
Kabir O. Akinyemi et al
Application of phage typing and pulsed-field gel electrophoresis to analyse Salmonella enterica isolates from a suspected outbreak in Lagos, Nigeria
Kabir O. Akinyemi et al
A recently started project exploring microbial populations attaching to plastic when inmersed in the ocean. Floating plastic (#2, #4, and #5) are deployed in coastal waters for varying times, and DNA extracted for 16S rRNA metagenomic sequencing. Validation of the sampling methodology and preliminary data are shown.
Presentation: Isolation and Characterization of Bacteria from Tropical SoilsNicole Rivera
The purpose of this experiment is to encounter bacteria from tropical soils that have the capability of producing antibiotic. In order to do so, it was necessary to isolate the bacteria, purify it five times and freeze it at a temperature of -80˚C. After purification, gram staining was performed to classify the bacteria as gram positive or negative. The bacteria were characterized by doing purification of genomic DNA and then, a Polymerase Chain Reaction (PCR) with the primers 16sRNA, 16S_1510R and 16S_8F. An electrophoresis was conducted to see if the PCR process went well. The PCR product was purified and analyzed by using Bioinformatics. Finally, both bacteria’s S15UPRCRISENRE30M01 and S15UPRCRISEAFD30M01A had positive results as antibiotic producers for M.luteus.
A recently started project exploring microbial populations attaching to plastic when inmersed in the ocean. Floating plastic (#2, #4, and #5) are deployed in coastal waters for varying times, and DNA extracted for 16S rRNA metagenomic sequencing. Validation of the sampling methodology and preliminary data are shown.
Presentation: Isolation and Characterization of Bacteria from Tropical SoilsNicole Rivera
The purpose of this experiment is to encounter bacteria from tropical soils that have the capability of producing antibiotic. In order to do so, it was necessary to isolate the bacteria, purify it five times and freeze it at a temperature of -80˚C. After purification, gram staining was performed to classify the bacteria as gram positive or negative. The bacteria were characterized by doing purification of genomic DNA and then, a Polymerase Chain Reaction (PCR) with the primers 16sRNA, 16S_1510R and 16S_8F. An electrophoresis was conducted to see if the PCR process went well. The PCR product was purified and analyzed by using Bioinformatics. Finally, both bacteria’s S15UPRCRISENRE30M01 and S15UPRCRISEAFD30M01A had positive results as antibiotic producers for M.luteus.
Enterprise Excellence is Inclusive Excellence.pdfKaiNexus
Enterprise excellence and inclusive excellence are closely linked, and real-world challenges have shown that both are essential to the success of any organization. To achieve enterprise excellence, organizations must focus on improving their operations and processes while creating an inclusive environment that engages everyone. In this interactive session, the facilitator will highlight commonly established business practices and how they limit our ability to engage everyone every day. More importantly, though, participants will likely gain increased awareness of what we can do differently to maximize enterprise excellence through deliberate inclusion.
What is Enterprise Excellence?
Enterprise Excellence is a holistic approach that's aimed at achieving world-class performance across all aspects of the organization.
What might I learn?
A way to engage all in creating Inclusive Excellence. Lessons from the US military and their parallels to the story of Harry Potter. How belt systems and CI teams can destroy inclusive practices. How leadership language invites people to the party. There are three things leaders can do to engage everyone every day: maximizing psychological safety to create environments where folks learn, contribute, and challenge the status quo.
Who might benefit? Anyone and everyone leading folks from the shop floor to top floor.
Dr. William Harvey is a seasoned Operations Leader with extensive experience in chemical processing, manufacturing, and operations management. At Michelman, he currently oversees multiple sites, leading teams in strategic planning and coaching/practicing continuous improvement. William is set to start his eighth year of teaching at the University of Cincinnati where he teaches marketing, finance, and management. William holds various certifications in change management, quality, leadership, operational excellence, team building, and DiSC, among others.
RMD24 | Retail media: hoe zet je dit in als je geen AH of Unilever bent? Heid...BBPMedia1
Grote partijen zijn al een tijdje onderweg met retail media. Ondertussen worden in dit domein ook de kansen zichtbaar voor andere spelers in de markt. Maar met die kansen ontstaan ook vragen: Zelf retail media worden of erop adverteren? In welke fase van de funnel past het en hoe integreer je het in een mediaplan? Wat is nu precies het verschil met marketplaces en Programmatic ads? In dit half uur beslechten we de dilemma's en krijg je antwoorden op wanneer het voor jou tijd is om de volgende stap te zetten.
Discover the innovative and creative projects that highlight my journey throu...dylandmeas
Discover the innovative and creative projects that highlight my journey through Full Sail University. Below, you’ll find a collection of my work showcasing my skills and expertise in digital marketing, event planning, and media production.
Unveiling the Secrets How Does Generative AI Work.pdfSam H
At its core, generative artificial intelligence relies on the concept of generative models, which serve as engines that churn out entirely new data resembling their training data. It is like a sculptor who has studied so many forms found in nature and then uses this knowledge to create sculptures from his imagination that have never been seen before anywhere else. If taken to cyberspace, gans work almost the same way.
Personal Brand Statement:
As an Army veteran dedicated to lifelong learning, I bring a disciplined, strategic mindset to my pursuits. I am constantly expanding my knowledge to innovate and lead effectively. My journey is driven by a commitment to excellence, and to make a meaningful impact in the world.
VAT Registration Outlined In UAE: Benefits and Requirementsuae taxgpt
Vat Registration is a legal obligation for businesses meeting the threshold requirement, helping companies avoid fines and ramifications. Contact now!
https://viralsocialtrends.com/vat-registration-outlined-in-uae/
Putting the SPARK into Virtual Training.pptxCynthia Clay
This 60-minute webinar, sponsored by Adobe, was delivered for the Training Mag Network. It explored the five elements of SPARK: Storytelling, Purpose, Action, Relationships, and Kudos. Knowing how to tell a well-structured story is key to building long-term memory. Stating a clear purpose that doesn't take away from the discovery learning process is critical. Ensuring that people move from theory to practical application is imperative. Creating strong social learning is the key to commitment and engagement. Validating and affirming participants' comments is the way to create a positive learning environment.
Tata Group Dials Taiwan for Its Chipmaking Ambition in Gujarat’s DholeraAvirahi City Dholera
The Tata Group, a titan of Indian industry, is making waves with its advanced talks with Taiwanese chipmakers Powerchip Semiconductor Manufacturing Corporation (PSMC) and UMC Group. The goal? Establishing a cutting-edge semiconductor fabrication unit (fab) in Dholera, Gujarat. This isn’t just any project; it’s a potential game changer for India’s chipmaking aspirations and a boon for investors seeking promising residential projects in dholera sir.
Visit : https://www.avirahi.com/blog/tata-group-dials-taiwan-for-its-chipmaking-ambition-in-gujarats-dholera/
LA HUG - Video Testimonials with Chynna Morgan - June 2024Lital Barkan
Have you ever heard that user-generated content or video testimonials can take your brand to the next level? We will explore how you can effectively use video testimonials to leverage and boost your sales, content strategy, and increase your CRM data.🤯
We will dig deeper into:
1. How to capture video testimonials that convert from your audience 🎥
2. How to leverage your testimonials to boost your sales 💲
3. How you can capture more CRM data to understand your audience better through video testimonials. 📊
Attending a job Interview for B1 and B2 Englsih learnersErika906060
It is a sample of an interview for a business english class for pre-intermediate and intermediate english students with emphasis on the speking ability.
The world of search engine optimization (SEO) is buzzing with discussions after Google confirmed that around 2,500 leaked internal documents related to its Search feature are indeed authentic. The revelation has sparked significant concerns within the SEO community. The leaked documents were initially reported by SEO experts Rand Fishkin and Mike King, igniting widespread analysis and discourse. For More Info:- https://news.arihantwebtech.com/search-disrupted-googles-leaked-documents-rock-the-seo-world/
1. Isolation, Purification and
Characterization of Gêmeos and
Isinaayusferti, bacteriophages
Isolated from Tropical Soils
Amanda M. Icazatti Burtell, Fernando Pachecho Ocasio,
John Rivera Meléndez & Leyda Villagrasa Méndez
Dr. Michael Rubin
RISE Program
University of Puerto Rico at Cayey
2. Introduction
• What is a bacteriophage?
• What host bacteria are being used?
– Mycobacterium smegmatis
– Bacillus cereus
• Where can bacteriophages be found?
• What do bacteriophages need for living?
• Types of life cycles: Lytic vs. Lysogenic cycle
3. • Why are bacteriophages important?
Phages can be used in various fields of science such
as:
- Genetics - Cloning and mutation of DNA and
studies in genome
- Therapeutic uses for the creation of antibiotics
resistant to bacteria
• What is the purpose of this experiment?
4. Problem and Hypothesis
• Problem: Can novel and interesting
bacteriophages be isolated from the tropical
soils of Puerto Rico?
• Hypothesis: Both Mycobacteriphages and
Bacilophages will be found on the tropical
soils of Puerto Rico.
10. Gêmeos (bacilophage)
• Coordenates: Lat: 23' 36" 23 S Long: 46' 41" 51 W
• Temperature: 19˚C
• Details: Dark and damp soil
• Found in a populated area near a river
• Sao Paolo. Brazil
12. Sample Coordenates Details Depth Temperature Date Place
Sample
#1
LVM
Lat: 18.2333419
Long:
-66.0478104
Humid and
plain soil
2” 26˚C Feb/3/14 Caguas,
PR
Sample
# 2
LVM
LAT: 18.1192448
LONG:
-66.1667764
Dry and
lumpy soil
1” 25˚C Feb/16/
14
Cayey,
PR
Sample
#3
LVM
Lat: 18.2333419
Long:
-66.0478104
Humid and
plain soil
3” 27˚C Feb/23/
14
Caguas,
PR
13. Sample Coordenates Details Depth Temperatur
e
Date Place
Sample
#1
JLR
Lat: 18 Nᵒ
Long: 66 Wᵒ
Humid and
saturated
soil
1‘’ 31˚C Feb/3/14 Cidra,
PR
Sample
# 2
JLR
Lat: 18 Nᵒ
Long: 66 Wᵒ
Dry soil
under
trash
disposal
Surface
sample
32˚C Feb/21/14 Cidra,
PR
14. Isinaayusferti (mycobacteriophage)
Coordenates: Lat: 18˚ 14’ .0312 " N
Long: 66˚ 2' 52.116" W
Temperature: 27 ˚ C
Place: Caguas, Puerto Rico
Details: Humid and plain soil. Found near the
garbage in front of a house.
17. Conclusion
• The soil samples were collected and isolated
using the enrichment method.
• A bacillophage was found in Sao Paolo, Brazil
and was named Gêmeos.
• Third Purification Stock Filtrate (Gêmeos)
• A mycobacteriophage found in Caguas, Puerto
Rico was named Isinaayusferti.
• Concentrated Stock Phage (Isinaayusferti)
