TATA box is cis regulatory elements, which are present in DNA sequence.Its regulate the RNAP activity in gene expression.TATA box have significant regulatory effect on different metabolic pathway during stress condition.
TATA box is cis regulatory elements, which are present in DNA sequence.Its regulate the RNAP activity in gene expression.TATA box have significant regulatory effect on different metabolic pathway during stress condition.
photoperiodism its discovery,significance,classifications,mechanism,critical day length,quality of light, night break phenomenon,phytochrome.florigen,floering genes, circadian rhythm
Agenda 6 de juliol reunió científica anual de la xrb 2017 xrbiotech
Presentació dels guanyadors del ‘Pot d'Idees'. Coordinador Francisco Valero.
• 12:15 - 12:30 Primer premi: “Utilització de nanomaterials magnètics per a la millora de l'eficiència de separació de microalgues utilitzades en depuració d’aigües o en processos biotecnològics”, Xavier Font (Unitat d’Enginyeria Química, Biològica i Ambiental, UAB).
• 12:30 - 12:45 Finalista: “SUMO inhibitors as novel anti-cancer therapeutic drugs”, Zoila Babot (CRAG).
12:45 - 13:45 “Bioinformatics resources in the biotechnology landscape”, Dr. Alfonso Valencia, Director del Department de Ciències de la Vida del BSC.
Convocatoria pot d_idees_2017_final_fbg_bis_2xrbiotech
Convocatòria Pot Idees 2017 - Xarxa de Referència en Biotecnologia (XRB)
Bases de la convocatòria
Objectiu: Valorització de projectes innovadors i amb alt potencial de transferència en fase d’assaig pre-industrial o prova de concepte amb la finalitat de facilitar un pas endavant cara a la seva comercialització o cerca d’altres fons competitius que ajudin a desenvolupar el seu grau de maduresa.
Finalitat projectes / accions susceptibles de finançament: Els projectes, que podran disposar d’un pressupost màxim total de 10.000€ i mínim de 8.000 €, tindran una clara repercussió a curt-mig termini en favor dels sectors industrials i/o del grup de recerca (patents, contractes amb empreses, creació d’empreses, etc...). Podran abordar qualsevol temàtica dins de la biotecnologia i amb clars objectius de transferència i valorització.
Pressupost Total Màxim: 18.000€
Pressupost mínim per projecte: 8.000 €*
Pressupost màxim per projecte: 10.000 € *
Beneficiaris del finançament: Tots els investigadors adscrits a la XRB. No hi haurà límit de propostes per cada investigador però només podria ser finançada una d’elles. Seran els propis grups els encarregats de justificar econòmicament els projectes a la Fundació Bosch i Gimpera (FBG), que s'ocuparà de gestionar els pagaments i reemborsaments. Els grups de recerca seran els propietaris de tots els drets sobre els resultats del projecte.
Objectiu: Valorització de projectes innovadors i amb alt potencial de transferència en fase d’assaig pre-industrial, prova de concepte, etc... amb la finalitat de facilitar un pas endavant cara a la seva comercialització o cerca d’altres fons competitius que ajudin a desenvolupar el seu grau de maduresa.
Finalitat projectes / accions susceptibles de finançament: Els projectes, que podran disposar d’un pressupost màxim total de 10.000€ i mínim de 8.000 €, tindran una clara repercussió a curt-mig termini en favor dels sectors industrials i/o del grup de recerca (patents, contractes amb empreses, creació d’empreses, etc...). Podran abordar qualsevol temàtica dins de la biotecnologia i amb clars objectius de transferència i valorització.
El biogàs del futur s’alimenta de ferro. Applied Nanoparticles, una empresa sorgida de la UAB, triplica el gas produït a les plantes de residus.
Article a La Vanguardia
El biogás del futuro se alimenta de hierroxrbiotech
El biogás del futuro se alimenta de hierro. Applied Nanoparticles, empresa surgida de la UAB, triplica el gas producido en las plantas de residuos.
Artículo en La Vanguardia
Ref 18 2014 CRAG responsable area projectes xrbiotech
Presentació de candidatures:
Carta de Presentació: Fent explícita la Referència de la plaça a la que s’opta i la motivació personal de la
sol∙licitud, adjuntant‐hi el Currículum Vitae, la documentació acreditativa dels mèrits exposats i de la titulació
obtinguda així com les aspiracions econòmiques i tres referències.
Termini de Presentació: Des de la publicació d’aquesta convocatòria fins el 23 d’octubre 2014.
Totes les candidatures s’han de registrar a través de la web del CRAG: http://www.cragenomica.es/jobs
Exploiting tertiary structure through local folds for crystallographic phasingxrbiotech
Exploiting tertiary structure through local folds for crystallographic phasing
Massimo Sammito, Claudia Millán, Dayté D Rodríguez, Iñaki M de Ilarduya, Kathrin Meindl, Ivan De Marino, Giovanna Petrillo, Rubén M Buey, José M de Pereda, Kornelius Zeth, George M Sheldrick and Isabel Usón
Journal:Nature Methods 10, 1099-1101 (2013); doi:10.1038/nmeth.2644
photoperiodism its discovery,significance,classifications,mechanism,critical day length,quality of light, night break phenomenon,phytochrome.florigen,floering genes, circadian rhythm
Agenda 6 de juliol reunió científica anual de la xrb 2017 xrbiotech
Presentació dels guanyadors del ‘Pot d'Idees'. Coordinador Francisco Valero.
• 12:15 - 12:30 Primer premi: “Utilització de nanomaterials magnètics per a la millora de l'eficiència de separació de microalgues utilitzades en depuració d’aigües o en processos biotecnològics”, Xavier Font (Unitat d’Enginyeria Química, Biològica i Ambiental, UAB).
• 12:30 - 12:45 Finalista: “SUMO inhibitors as novel anti-cancer therapeutic drugs”, Zoila Babot (CRAG).
12:45 - 13:45 “Bioinformatics resources in the biotechnology landscape”, Dr. Alfonso Valencia, Director del Department de Ciències de la Vida del BSC.
Convocatoria pot d_idees_2017_final_fbg_bis_2xrbiotech
Convocatòria Pot Idees 2017 - Xarxa de Referència en Biotecnologia (XRB)
Bases de la convocatòria
Objectiu: Valorització de projectes innovadors i amb alt potencial de transferència en fase d’assaig pre-industrial o prova de concepte amb la finalitat de facilitar un pas endavant cara a la seva comercialització o cerca d’altres fons competitius que ajudin a desenvolupar el seu grau de maduresa.
Finalitat projectes / accions susceptibles de finançament: Els projectes, que podran disposar d’un pressupost màxim total de 10.000€ i mínim de 8.000 €, tindran una clara repercussió a curt-mig termini en favor dels sectors industrials i/o del grup de recerca (patents, contractes amb empreses, creació d’empreses, etc...). Podran abordar qualsevol temàtica dins de la biotecnologia i amb clars objectius de transferència i valorització.
Pressupost Total Màxim: 18.000€
Pressupost mínim per projecte: 8.000 €*
Pressupost màxim per projecte: 10.000 € *
Beneficiaris del finançament: Tots els investigadors adscrits a la XRB. No hi haurà límit de propostes per cada investigador però només podria ser finançada una d’elles. Seran els propis grups els encarregats de justificar econòmicament els projectes a la Fundació Bosch i Gimpera (FBG), que s'ocuparà de gestionar els pagaments i reemborsaments. Els grups de recerca seran els propietaris de tots els drets sobre els resultats del projecte.
Objectiu: Valorització de projectes innovadors i amb alt potencial de transferència en fase d’assaig pre-industrial, prova de concepte, etc... amb la finalitat de facilitar un pas endavant cara a la seva comercialització o cerca d’altres fons competitius que ajudin a desenvolupar el seu grau de maduresa.
Finalitat projectes / accions susceptibles de finançament: Els projectes, que podran disposar d’un pressupost màxim total de 10.000€ i mínim de 8.000 €, tindran una clara repercussió a curt-mig termini en favor dels sectors industrials i/o del grup de recerca (patents, contractes amb empreses, creació d’empreses, etc...). Podran abordar qualsevol temàtica dins de la biotecnologia i amb clars objectius de transferència i valorització.
El biogàs del futur s’alimenta de ferro. Applied Nanoparticles, una empresa sorgida de la UAB, triplica el gas produït a les plantes de residus.
Article a La Vanguardia
El biogás del futuro se alimenta de hierroxrbiotech
El biogás del futuro se alimenta de hierro. Applied Nanoparticles, empresa surgida de la UAB, triplica el gas producido en las plantas de residuos.
Artículo en La Vanguardia
Ref 18 2014 CRAG responsable area projectes xrbiotech
Presentació de candidatures:
Carta de Presentació: Fent explícita la Referència de la plaça a la que s’opta i la motivació personal de la
sol∙licitud, adjuntant‐hi el Currículum Vitae, la documentació acreditativa dels mèrits exposats i de la titulació
obtinguda així com les aspiracions econòmiques i tres referències.
Termini de Presentació: Des de la publicació d’aquesta convocatòria fins el 23 d’octubre 2014.
Totes les candidatures s’han de registrar a través de la web del CRAG: http://www.cragenomica.es/jobs
Exploiting tertiary structure through local folds for crystallographic phasingxrbiotech
Exploiting tertiary structure through local folds for crystallographic phasing
Massimo Sammito, Claudia Millán, Dayté D Rodríguez, Iñaki M de Ilarduya, Kathrin Meindl, Ivan De Marino, Giovanna Petrillo, Rubén M Buey, José M de Pereda, Kornelius Zeth, George M Sheldrick and Isabel Usón
Journal:Nature Methods 10, 1099-1101 (2013); doi:10.1038/nmeth.2644
Explore natural remedies for syphilis treatment in Singapore. Discover alternative therapies, herbal remedies, and lifestyle changes that may complement conventional treatments. Learn about holistic approaches to managing syphilis symptoms and supporting overall health.
New Directions in Targeted Therapeutic Approaches for Older Adults With Mantl...i3 Health
i3 Health is pleased to make the speaker slides from this activity available for use as a non-accredited self-study or teaching resource.
This slide deck presented by Dr. Kami Maddocks, Professor-Clinical in the Division of Hematology and
Associate Division Director for Ambulatory Operations
The Ohio State University Comprehensive Cancer Center, will provide insight into new directions in targeted therapeutic approaches for older adults with mantle cell lymphoma.
STATEMENT OF NEED
Mantle cell lymphoma (MCL) is a rare, aggressive B-cell non-Hodgkin lymphoma (NHL) accounting for 5% to 7% of all lymphomas. Its prognosis ranges from indolent disease that does not require treatment for years to very aggressive disease, which is associated with poor survival (Silkenstedt et al, 2021). Typically, MCL is diagnosed at advanced stage and in older patients who cannot tolerate intensive therapy (NCCN, 2022). Although recent advances have slightly increased remission rates, recurrence and relapse remain very common, leading to a median overall survival between 3 and 6 years (LLS, 2021). Though there are several effective options, progress is still needed towards establishing an accepted frontline approach for MCL (Castellino et al, 2022). Treatment selection and management of MCL are complicated by the heterogeneity of prognosis, advanced age and comorbidities of patients, and lack of an established standard approach for treatment, making it vital that clinicians be familiar with the latest research and advances in this area. In this activity chaired by Michael Wang, MD, Professor in the Department of Lymphoma & Myeloma at MD Anderson Cancer Center, expert faculty will discuss prognostic factors informing treatment, the promising results of recent trials in new therapeutic approaches, and the implications of treatment resistance in therapeutic selection for MCL.
Target Audience
Hematology/oncology fellows, attending faculty, and other health care professionals involved in the treatment of patients with mantle cell lymphoma (MCL).
Learning Objectives
1.) Identify clinical and biological prognostic factors that can guide treatment decision making for older adults with MCL
2.) Evaluate emerging data on targeted therapeutic approaches for treatment-naive and relapsed/refractory MCL and their applicability to older adults
3.) Assess mechanisms of resistance to targeted therapies for MCL and their implications for treatment selection
Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Prix Galien International 2024 Forum ProgramLevi Shapiro
June 20, 2024, Prix Galien International and Jerusalem Ethics Forum in ROME. Detailed agenda including panels:
- ADVANCES IN CARDIOLOGY: A NEW PARADIGM IS COMING
- WOMEN’S HEALTH: FERTILITY PRESERVATION
- WHAT’S NEW IN THE TREATMENT OF INFECTIOUS,
ONCOLOGICAL AND INFLAMMATORY SKIN DISEASES?
- ARTIFICIAL INTELLIGENCE AND ETHICS
- GENE THERAPY
- BEYOND BORDERS: GLOBAL INITIATIVES FOR DEMOCRATIZING LIFE SCIENCE TECHNOLOGIES AND PROMOTING ACCESS TO HEALTHCARE
- ETHICAL CHALLENGES IN LIFE SCIENCES
- Prix Galien International Awards Ceremony
These lecture slides, by Dr Sidra Arshad, offer a quick overview of physiological basis of a normal electrocardiogram.
Learning objectives:
1. Define an electrocardiogram (ECG) and electrocardiography
2. Describe how dipoles generated by the heart produce the waveforms of the ECG
3. Describe the components of a normal electrocardiogram of a typical bipolar leads (limb II)
4. Differentiate between intervals and segments
5. Enlist some common indications for obtaining an ECG
Study Resources:
1. Chapter 11, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 9, Human Physiology - From Cells to Systems, Lauralee Sherwood, 9th edition
3. Chapter 29, Ganong’s Review of Medical Physiology, 26th edition
4. Electrocardiogram, StatPearls - https://www.ncbi.nlm.nih.gov/books/NBK549803/
5. ECG in Medical Practice by ABM Abdullah, 4th edition
6. ECG Basics, http://www.nataliescasebook.com/tag/e-c-g-basics
Couples presenting to the infertility clinic- Do they really have infertility...Sujoy Dasgupta
Dr Sujoy Dasgupta presented the study on "Couples presenting to the infertility clinic- Do they really have infertility? – The unexplored stories of non-consummation" in the 13th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2024) at Manila on 24 May, 2024.
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
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Dr Pablo Leivar (CRAG) - THE DARK SIDE OF PLANT GROWTH: A ROLE FOR PHYTOCHROME-INTERACTING TRANSCRIPTION FACTORS
1. The da
e dark s de o p a
side of plant g o
growth:
a role for phytochrome-interacting
transcription factors
p
PABLO LEIVAR
XARXA DE REFERÈNCIA EN
BIOTECNOLOGIA
Barcelona, Juliol 2012
Centre de Recerca en
C t d R
Agrigenòmica (CRAG)
2. How do plants grow and develop in accordance to their ambient
light/DARK conditions?
g
- Introduction: phytochrome (phy) photoreceptors and PIF transcription factors
- Research lines: Seedling deetiolation / Diurnal growth
- Biotechnology p
gy potential
Dark Light
*seedlings
=plántulas
PHOTOMORPHOGENESIS
3. photosynthesis environmental
information
Light as Energy for photosynthesis and
as a source of Information
4. Light and photomorphogenesis
DAY
Intensity
Direction (photosynthetic
(phototropism) adaptation)
)
Color
( g
(neighbor
(germination) (deetiolation)
perception)
LIGHT (shade avoidance)
seed seedling juvenile adult
DARK
Darkness duration
or photoperiod
(flowering)
NIGHT
6. The Red (R)/Far Red (FR) sensing
phytochrome (phy) photoreceptors
VISIBLE SPECTRUM
Wavelength (nm)
Pr Pfr
7. phy act as molecular switches in response to R and FR
Red
(660 nm)
Physiological
Synthesis
responses
phy Pr phy Pfr
(Inactive) (Active)
Far Red
(
(730 nm))
Slow Dark
Reversion
8. How does the phy regulate plant morphogenesis in response to
changes in ambient light/dark conditions?
Red
(660 nm)
- SEEDLING
DARK/LIGHT DEETIOLATION
TRANSITIONS
- DIURNAL
GROWTH
phy Pr phy Pfr
(Inactive) (Active)
Far Red
(
(730 nm))
Slow Dark
Reversion
9. Seedling Deetiolation Diurnal Growth
(day/night y
( y g cycles) )
Cotyledons
(embryonic leaves)
Hypocotyl
(young stem)
10 mm
4 days seedlings after germination at 22º
Arabidopsis
thaliana
0.5 mm
10. Seedling Deetiolation Diurnal Growth
(day/night y
( y g cycles) )
Etiolated
growth
10 mm
Deetiolation
phyB
DARK RED
(D) (R)
R
Pr Pfr
Etiolated
Eti l t d
growth
11. Seedling Deetiolation Diurnal Growth
(day/night y
( y g cycles) )
Etiolated
growth
SD-induced
growth
10 mm
Deetiolation
DARK RED Short-Days W
(D) (R) (SD: 16hD-8hW)
(day)
R How does the phy R
Pr Pfr transmit the primary Pr Pfr
D
light signal (night)
Etiolated
Eti l t d information? Hypocotyl
H t l
growth growth
12. Seedling Deetiolation
Etiolated
growth Seedling deetiolation as
a model system
d l t
10 mm
Deetiolation to study responses to
Light/Dark
DARK RED
(D) (R)
R How does the phy
Pr Pfr transmit the primary
light signal
Etiolated
Eti l t d information?
growth
13. PIF3 and related PIFs are phy-interacting bHLH transcription
factors that selectively bind the Pfr form of the phy
Nuclear
Localization Signal
g
NLS
PIF3
PIFs 1,4,5
, ,
APA/B bHLH
phy Pfr Basic
Binding Helix-loop-helix
Red
GAD:PIF3
(660 nm)
Preferential binding to
PIF3 the DNA motif G-BOX:
phyB CACGTG
GAD: (Martínez-García et al., 2000, Science)
PIF3
phy Pr phy Pfr
(Inactive)
+FR
(Active) +R
+
Far Red
+
(730 nm) (Ni et al., 1999, Nature)
14. Photoactivated phy triggers rapid pifq mutants lacking PIFs 1, 3,
proteolytic degradation of PIFs 1, 4 and 5 show a constitutively
y
3, 4 and 5 in response to light photomorphogenic phenotype
PIFs
R light
D + R (min)
Etiolated
0 10 20 30 40 50 60 Growth
phy
PIF3
(Al-Sady et al., 2006, Molecular Cell)
PIFs
PIF3:CFP PHYB:YFP Overlay
WT pifq
(Bauer et al, 2004, The Plant Cell)
(pif1pif3pif4pif5)
(Leivar et al., 2008, Current Biology)
15. PIF1, 3, 4 and 5 accumulate in the dark
and sustain etiolated growth
CYTOPLASM
Dark
PIF1
Pr PIF3
PIF4
PIF5
PIF-regulated
PIF regulated
G-box
genes
NUCLEUS
Transcriptional PIF-regulated Etiolated
network transcriptome Growth
~1000 genes
1000
16. Initiation of seedling deetiolation through
phy-induced degradation of PIFs 1 3 4 5
1,3,4,5
CYTOPLASM
SE?
E3 LIGAS
KINASE?
?
Red 26S proteasome
induced Dark Light
R P Ub degradation
R PIF1 PIF1 PIF1
P Ub
Pr PIF3 PIF3 PIF3
Pfr P Ub
PIF4 PIF4 PIF4
PIF5
P PIF5
Ub
PIF5
PIF-regulated
PIF regulated
G-box
genes
NUCLEUS
Signal Transcriptional Transcriptional Etiolated Seedling
Perception network reprogramming Growth Deetiolation
& (Photomorphogenesis)
Nuclear
translocation
17. The Dark
Side of Plant
Seedling Deetiolation growth
R
Pr Pfr PIFs
PIF
Transcriptional
network
Etiolated
Growth
18. How do plants grow in accordance
to their ambient light/DARK conditions?
Seedling Deetiolation
R
Pr Pfr PIFs
PIF
Transcriptional
network
Etiolated
Growth
RESEARCH LINE 1:
Seedling growth in the Dark after germination:
Identification of new regulators of deetiolation acting
downstream of the PIFs
1a- Functional profiling of PIF3-regulated genes
1b- Suppressor screen of pifq mutants
19. 1a- Functional Profiling of PIF3-regulated genes
I. Identification of PIF3-regulated MIDA II. Selection candidate
(Misexpressed In DArk) genes regulatory genes
-1.35000 0 1.35000
Z-score
- 13 MIDA genes selected
(MIDA1-MIDA13)
D0h_Rep1
D1h_Rep1
D1h_Rep2
D1h_Rep3
WT D0h_Rep3
- Identification of insertional
D0h_Rep2
D0h_Rep1
D0h_Rep3
D1h_Rep1
pif3 D1h_Rep3
D1h Rep3
mutants in public collections
D0h_Rep2
D1h_Rep2
42 Induced Genes 40 Repressed Genes (mida1-mida13)
2.5 1.2
Average FC
Average FC
2 1
1.5 0.8
0.6
1
0.4
0.5 0.2
0 0
WT pif3 WT pif3
III. Phenotypic analysis
of mida mutants.
(Sentandreu et al., 2011, Plant Cell)
20. 1a- Functional Profiling of PIF3-regulated genes
PIF3-regulated transcriptional network
implement organ-specific responses
during seedling deetiolation
g g
Maria Sentandreu
Next:
Characterization of the
molecular function of
the MIDAs during
seedling deetiolation.
g
(Sentandreu et al., 2011, Plant Cell)
(Sentandreu et al., 2011, Plant Signaling and Behavior)
22. 1b- Suppressor screen of pifq mutants
Transformation with a Full-Length
overexpression cDNA (FOX) library
(Ichikawa et al., 2006, Plant Journal 45: 974)
Screen in dark in the presence of hygromycin
WT pifq
(pif1pif3pif4pif5)
pifq pifq HygR
Next:
HygR
Validation of sop mutants. Putative sops
Identification of responsible genes.
(suppressors-of-pifq)
23. How do plants grow in accordance
to their ambient light/DARK conditions?
Seedling Deetiolation Diurnal Growth
(day)
R
R
Pr Pfr PIFs
PIF Pfr Pr
D
(night)
Transcriptional
network
Etiolated Hypocotyl
Growth Growth
RESEARCH LINE 1: RESEARCH LINE 2:
Seedling growth in the Dark after germination: Seedling growth in the Dark in diurnal conditions
Identification of new regulators of deetiolation acting (8h Light:16h Dark)
downstream of the PIFs
2a- Characterization of PIF3 function
1a- Functional profiling of PIF3-regulated genes
2b- Definition of PIF-regulated transcriptional
g p
1b- Suppressor screen of pifq mutants networks implementing growth responses to light
24. 2a- Characterization of PIF3 function in diurnal growth
0h 8h 24h
Day Night
(Nozue et al 2007 Nature)
al., 2007,
Hypocotyl growth rate peaks at the end of the night
0.25‐
0.2‐ WT
ate
Growth Ra
(mmh-1)
0.15‐
0.1‐
Judit
0.05‐
Soy
0‐
pif3
3 6 9 12 15 18 21 24 27
Time (h)
(Soy et al., 2012, Plant Journal)
25. 2a- Characterization of PIF3 function in short-days
0h 8h 24h II. PIF3 induces the expression of
growth-related at the end-of the night
10-
I. PIF3 protein accumulates at
p
vel
Expression Lev
8
8- PIL1
the end of the night in short-days WT
Relative
6-
WT pif3 4-
h: 3 6 8 10 12 14 16 18 20 22 23 24 25 23 pif3
2-
PIF3
n.s 0-
3 6 9 12 15 18 21 24
n.s
Time (h)
ponceau 7
ent
6 PIL1p
ChIP qRT-PCR
old-Enrichme
5
4
3
PIF3 2
PIF4/5 1
Fo
C
0
PIL1 NoAb Ab NoAb Ab NoAb Ab NoAb Ab
XTR7 promoter control
region promoter
control
region
phyA, HFR1 WT YFP-PIF3
phyB phyB phyB
phytochrome-Imposed oscillations of PIF3
Hypocotyl
yp y abundance regulate hypocotyl growth
elongation under diurnal conditions in conjunction
Day Night with PIF4/5 and the circadian clock
(Soy et al., 2012, Plant Journal)
26. 2b- Definition of PIF-regulated transcriptional networks
implementing growth responses to light
(Leivar et al., 2009 Pl t C ll)
(L i t l 2009, Plant Cell)
Comparative
transcriptomic Deetiolation
(WT vs pifq)
analysis (839 genes)
729
11 76 Simulated
Diurnal 23
(WT vs
shade
(WT vs pifq)
pif4pif5) 66 148
18 (265 genes)
(118 genes)
(Nozue et al., 2011, Plant Physiology)
(Leivar et al., 2012, Plant Cell)
27. Seedling deetiolation Diurnal conditions
D R SD W
(8hW-16hD)
R Day
Pr Pfr Pr Pfr
Night
Pr
PIFs
G-box
cellular machinery that implement
different facets of growth
28. Seedling deetiolation Diurnal conditions
D R SD W
(8hW-16hD)
R Day
Pr Pfr Pr Pfr
Night
The balance between phy
Pfr
Pf and PIFs determines plant
growth during light/dark
PIFs transitions
G-box
cellular machinery that implement
different facets of growth
29. Aplicaciones y potencial biotecnológico del sistema del fitocromo
1. Obtención de variedades que muestran alteraciones en las respuestas a la luz para
mejorar procesos agrícolas
- Revolución verde: Selección de variedades de cereales semi-enanas, portadoras de alelos que modulan
la señalización por luz, y que muestran un crecimiento reducido a cambio de una mayor producción de
semillas (p.ej. alelo Reduced height1 (Rht1) en arroz).
2- Ingeniería molecular basada en el fitocromo
OFF ON
2.1- Generación de interruptores moleculares, reversibles, y
PIF Pr +R PIF Pfr
modulables por luz:
+
- Expresión génica
g X Y X Y
+FR
- Splicing de proteínas
(Shimizu et al., 2002, Nat. Biotech.)
- Señalización en dominios subcelulares discretos (Tyszkiewicz et al., 2008, Nat. Methods)
proceso
2.2- Generación de nuevos marcadores fluorescentes (Levskaya et al., 2009, Nature) celular
basados en el fitocromo que emiten en infrarrojo
(Shu et al., 2009, Science)
3- Regulación por luz, fitocromo y PIFs del metabolismo lipídico
g p , p
en semillas y plántulas
Potencial aplicado:
- Producción de ácidos grasos poli-insaturados con valor nutracéutico
- Producción de aceites que actúen como biodiesel
Céline Diaz
30. Roger Miralles
Guiomar Martín
Elena Monte
El M t Céline Diaz
Maria Judit Soy
Sentandreu
Old members
Nahuel González At UC-Berkeley
Peter H. Quail
Funding
Beatriu de Pinós Fellowship (Generalitat de Catalunya) At CNB-CSIC
Marie Curie Actions IRG PIRG06-GA-2009–256420
Salomé Prat
Ministerio de Ciencia e I
Mi i t i d Ci i Innovación (MICINN)
ió
AGAUR 2009 SGR 206