1) The document describes the treatment of two patients, R and K, with chronic wounds using fetal stem cells.
2) Both patients had non-healing ulcers/wounds for over 3 months. They underwent a 4 step treatment involving intravenous and local injections of various fetal stem cells around the wounds as well as application of fetal tissue extracts.
3) For patient R, full wound closure was not achieved but signs of healing like epithelialization were observed over 46 days. For patient K, the wound showed continuous epithelialization and was nearly closed by 46 days with the aid of fetal stem cell therapy and antibiotics for infections.
Role of Stem Cells in Obstetrics and Gynecology PracticeAsha Jain
Role of Stem Cells in Obstetrics and Gynecology Practice
Talk delivered at 4th Biennial International ISCSGCON 2021
on Febuary 13,2021 by Dr. Asha Jain
Los días 20 y 21 de octubre de 2016, la Fundacion Ramón Areces organizó un simposio internacional para analizar las 'Enfermedades raras de la piel: de la clínica al gen y viceversa'. El doctor Fernando Larcher Laguzzi, del CIEMAT-Universidad Carlos III de Madrid-IIS Fundación Jiménez Díaz, ejerció de coordinador.
The study aimed to evaluate morphometric and immunohistochemical changes in the umbilical cord of patients with placenta previa compared to normotensive patients. Samples were taken from 20 placenta previa patients and 20 healthy pregnant women aged 35-38 weeks. Morphometric examination found significantly different endothelial cell length, fibroblast cell length, smooth muscle cell length, and vessel wall thickness between groups. Immunohistochemical analysis revealed increased ADAMTS-9 and VEGF-A expression and decreased E-cadherin expression in the placenta previa group, suggesting changes in extracellular matrix dynamics and intercellular adhesion that may impact fetal development. The results indicate ADAMTS-9 plays an important role in cellular regulation
The document discusses the use of bone marrow stromal stem cells (bMSCs) therapy for musculoskeletal problems like discs, cartilage, and bone. It describes a study conducted in rabbits to evaluate the effectiveness of transplanting bMSCs and distracting the intervertebral disc to reverse disc degeneration. The study found that bMSCs transplantation combined with disc distraction led to improved disc height, higher proteoglycan content, and better histological scores compared to other treatment groups. The results suggest that bMSCs transplantation along with increasing disc rehydration through distraction can promote extracellular matrix repair and regeneration for intervertebral disc degeneration.
Cartilage Repair using Stem cell & OrthobiologicsVaibhav Bagaria
Regenerating Cartilage is a challenge. What's new in this field of cartilage regeneration and the current status of the stem cell use in this field is described.
Company has been created for scientific elaboration and practical application of new biotechnological methods in human disease treatment, originated on applying fetal stem cells and tissues extract.
Company in its development reviews elaborating new biotechnological rejuvenating creams on the origin of placental extract, likewise embedding in clinic the anti-aging biotechnological complex.
Administration of Autologous Bone Marrow Stem Cells Into Spinal Cord Injury P...◂ Justin (M) Gaines ▸
This document summarizes a study that administered autologous bone marrow stem cells (BMSCs) via multiple routes (directly into the spinal cord, directly into the spinal canal, and intravenous) to 8 spinal cord injury patients. The study found that administering BMSCs via multiple routes was safe and improved patients' quality of life based on evaluations using scales like ASIA, Barthel, Frankel, and a new bladder function scale. To date, administering BMSCs to 52 spinal cord injury patients has had no cases of tumor formation, infection, or increased pain and few minor adverse events.
Autologous Mesenchymal Stem Cells in OrthopaedicsVladimir Bobic
Nuffield Health, The Grosvenor Hospital Chester, UK
27 June 2013. GP and Physiotherapy Seminar: Autologous Stem Cell Therapies in Orthopaedics. Moderator and Presenter: Vladimir Bobic, Chester Knee Clinic
Role of Stem Cells in Obstetrics and Gynecology PracticeAsha Jain
Role of Stem Cells in Obstetrics and Gynecology Practice
Talk delivered at 4th Biennial International ISCSGCON 2021
on Febuary 13,2021 by Dr. Asha Jain
Los días 20 y 21 de octubre de 2016, la Fundacion Ramón Areces organizó un simposio internacional para analizar las 'Enfermedades raras de la piel: de la clínica al gen y viceversa'. El doctor Fernando Larcher Laguzzi, del CIEMAT-Universidad Carlos III de Madrid-IIS Fundación Jiménez Díaz, ejerció de coordinador.
The study aimed to evaluate morphometric and immunohistochemical changes in the umbilical cord of patients with placenta previa compared to normotensive patients. Samples were taken from 20 placenta previa patients and 20 healthy pregnant women aged 35-38 weeks. Morphometric examination found significantly different endothelial cell length, fibroblast cell length, smooth muscle cell length, and vessel wall thickness between groups. Immunohistochemical analysis revealed increased ADAMTS-9 and VEGF-A expression and decreased E-cadherin expression in the placenta previa group, suggesting changes in extracellular matrix dynamics and intercellular adhesion that may impact fetal development. The results indicate ADAMTS-9 plays an important role in cellular regulation
The document discusses the use of bone marrow stromal stem cells (bMSCs) therapy for musculoskeletal problems like discs, cartilage, and bone. It describes a study conducted in rabbits to evaluate the effectiveness of transplanting bMSCs and distracting the intervertebral disc to reverse disc degeneration. The study found that bMSCs transplantation combined with disc distraction led to improved disc height, higher proteoglycan content, and better histological scores compared to other treatment groups. The results suggest that bMSCs transplantation along with increasing disc rehydration through distraction can promote extracellular matrix repair and regeneration for intervertebral disc degeneration.
Cartilage Repair using Stem cell & OrthobiologicsVaibhav Bagaria
Regenerating Cartilage is a challenge. What's new in this field of cartilage regeneration and the current status of the stem cell use in this field is described.
Company has been created for scientific elaboration and practical application of new biotechnological methods in human disease treatment, originated on applying fetal stem cells and tissues extract.
Company in its development reviews elaborating new biotechnological rejuvenating creams on the origin of placental extract, likewise embedding in clinic the anti-aging biotechnological complex.
Administration of Autologous Bone Marrow Stem Cells Into Spinal Cord Injury P...◂ Justin (M) Gaines ▸
This document summarizes a study that administered autologous bone marrow stem cells (BMSCs) via multiple routes (directly into the spinal cord, directly into the spinal canal, and intravenous) to 8 spinal cord injury patients. The study found that administering BMSCs via multiple routes was safe and improved patients' quality of life based on evaluations using scales like ASIA, Barthel, Frankel, and a new bladder function scale. To date, administering BMSCs to 52 spinal cord injury patients has had no cases of tumor formation, infection, or increased pain and few minor adverse events.
Autologous Mesenchymal Stem Cells in OrthopaedicsVladimir Bobic
Nuffield Health, The Grosvenor Hospital Chester, UK
27 June 2013. GP and Physiotherapy Seminar: Autologous Stem Cell Therapies in Orthopaedics. Moderator and Presenter: Vladimir Bobic, Chester Knee Clinic
This document discusses the importance of using accurate 3D modeling data for planning and optimizing wireless networks such as 3G, 4G, LTE, and WiMax networks. It notes that the level of detail and accuracy of the modeling data directly impacts the accuracy of network planning, which can reduce network rollout and optimization costs for operators. Higher quality 3D modeling data that accounts for terrain and urban obstacles can increase the distance between base stations by 25-30% while maintaining network quality. The document promotes the 3D modeling services of Visicom, a Ukrainian company, for wireless network planning.
Equally Yoked - Biblical counseling - Liberal Arts - HumanitiesJohnNevinsAndrews1874
The document discusses the concept of being "equally yoked" from a biblical perspective. It provides several bible verses regarding being equally yoked and definitions of social identity, civil identity, personal identity, and suggestions for liberal arts schools that align with biblical principles of being equally yoked. The overall message is that Christians should only be in relationships where both individuals share the same core biblical beliefs and values.
Our consultants designed a self-service check-in kiosk to reduce workload for hotel receptionists and speed up the check-in process for frequent travelers. User research involved interviews and observations at various hotels. The resulting design uses smart cards for a 3-step check-in/out process and addresses accessibility, speed, and workload reduction through considerations for positioning, lighting, and allowing simultaneous check-ins. User testing was conducted and the final recommendations included a key dispenser and optional anti-glare shield.
The document discusses the challenges of developing offshore oil and gas fields, particularly in deep waters and remote locations. It notes that while offshore development technologies have enabled projects in up to 3,000m of water, developing fields in ultra-deep waters and frontier regions requires further technological advances. Specifically, it focuses on the need for high-capacity pipe-lay vessels using J-lay or reel-lay methods to install thick-walled pipes in these challenging environments, with each method having advantages depending on the characteristics of the specific field development project. Saipem's new flexible lay and construction vessel, the Normand Maximus, is presented as an innovative vessel designed to aid in ultra-deep water SURF installation and field
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive functioning. Exercise causes chemical changes in the brain that may help protect against mental illness and improve symptoms.
Dr. Kenneth Dickie from Royal Centre of Plastic Surgery in Barrie, Ontario explained the use of stem cells technology in plastic surgery.
If you have any questions, please contact Dr. Kenneth Dickie at http://royalcentreofplasticsurgery.com/
Cultured skin substitutes prepared from cultured skin cells and biopolymers can reduce the need for donor skin grafts and have been shown to effectively treat excised burns, burn scars, and congenital skin lesions. Cultured skin substitutes generate skin phenotypes in the lab and restore tissue function and systemic homeostasis when implanted. Healed skin from cultured skin substitutes is smooth, soft and strong, though pigmentation may be irregular. Cultured skin substitutes close 67 times the area of donor skin compared to less than 4 times for split-thickness skin grafts, and result in similar qualitative outcomes.
This document summarizes the current state of stem cell therapy research for spinal cord injuries. It discusses the historical basis and developments in the field. It describes the two main types of cell-based therapies - regenerative cell therapy which aims to restore myelin and integrate axons, and replacement cell therapy which focuses on replacing neurons and oligodendrocytes. Various cell sources and delivery methods used in both regenerative and replacement therapies are outlined, including their advantages and limitations. The document also reviews several human clinical trials conducted to evaluate the safety and potential efficacy of different cell therapies.
This document contains multiple questions related to inflammation, immune responses, and wound healing.
1) The first question describes a case of mastitis in a new mother and asks which inflammatory cell would most likely be seen in breast fluid under the microscope.
2) The second question involves a boy who punctures his thumb with a rusty nail and develops swelling, asking which mechanism is primarily responsible for the initial swelling.
3) The third question describes a woman with pneumonia who develops a pleural effusion, asking which type of effusion is present based on characteristics provided.
4) The fourth question involves a boy with recurrent infections who has an impaired ability to generate reactive oxygen species, asking which protein
EWMA 2014 - EP457 AUTOTRANSPLANTATION OF THE ADIPOSE TISSUE DERIVED STEM CELL...EWMA
Autotransplantation of adipose tissue-derived stem cells shows promise for treating chronic wounds. In a study of 21 patients with diabetic foot or venous ulcers, 75% of venous ulcers and 76.9% of diabetic foot ulcers showed improvement after receiving autologous stem cells isolated from adipose tissue and implanted around and into the wounds. Laboratory results found that the stem cells expressed mesenchymal stem cell markers CD105, CD90, and CD73 both immediately after isolation and after one week in culture, suggesting adipose tissue is a suitable source of stem cells for cell therapy. However, the effects were fragile with single application, so further studies are needed to improve outcomes.
This document summarizes research on using autologous skeletal myoblasts (stem cells from skeletal muscle) in cellular cardiomyoplasty to repair damaged heart muscle following a myocardial infarction. Skeletal myoblasts are harvested from a muscle biopsy, expanded in culture, and then injected into the scarred heart muscle. Several clinical trials have shown promising results, with implanted cells orienting against cardiac stress and preventing thinning of the injured heart region. While further research is still needed, cellular cardiomyoplasty using autologous skeletal myoblasts shows potential as a definitive treatment for heart failure.
Further experience with the double onlay preputial flapasopahospital
This document describes the use of a double onlay preputial flap technique for repairing hypospadias in one stage. The authors reviewed 47 patients who underwent this procedure. Complications occurred in 12 patients (25%), including fistulas in 8 patients (17%), diverticula in 4 patients (9%), and other issues in fewer patients. Despite a high rate of more severe proximal hypospadias (75% penoscrotal or perineal), complication rates were comparable or lower than other techniques. The double onlay flap technique offers good cosmetic and functional results for repairing hypospadias.
Intra Cranial Stem Cell Transplant For Npc.Ppt 2Duriya Lakdawala
Finding a treatment for Niemann Pick Type C will provide hope not only to Aaditya Ravi Dasgupta and Tasneem Tankiwala in India but to many others like Addi and Cassi Hempel, Gabrielle Laverde and Peyton and Kayla Hadley in US, Husein Taher in Tanzania, South Africa, Roy Green in UK and so many more kids, teens and adults all over the world and in India that have not been diagnosed yet due to the cost and complexity of the diagnostic process. You can leave a wish for Aaditya (http://addiandcassi.com/guestbook) or for more information go to: www.HopeforAaditya.org
This document discusses the importance of using accurate 3D modeling data for planning and optimizing wireless networks such as 3G, 4G, LTE, and WiMax networks. It notes that the level of detail and accuracy of the modeling data directly impacts the accuracy of network planning, which can reduce network rollout and optimization costs for operators. Higher quality 3D modeling data that accounts for terrain and urban obstacles can increase the distance between base stations by 25-30% while maintaining network quality. The document promotes the 3D modeling services of Visicom, a Ukrainian company, for wireless network planning.
Equally Yoked - Biblical counseling - Liberal Arts - HumanitiesJohnNevinsAndrews1874
The document discusses the concept of being "equally yoked" from a biblical perspective. It provides several bible verses regarding being equally yoked and definitions of social identity, civil identity, personal identity, and suggestions for liberal arts schools that align with biblical principles of being equally yoked. The overall message is that Christians should only be in relationships where both individuals share the same core biblical beliefs and values.
Our consultants designed a self-service check-in kiosk to reduce workload for hotel receptionists and speed up the check-in process for frequent travelers. User research involved interviews and observations at various hotels. The resulting design uses smart cards for a 3-step check-in/out process and addresses accessibility, speed, and workload reduction through considerations for positioning, lighting, and allowing simultaneous check-ins. User testing was conducted and the final recommendations included a key dispenser and optional anti-glare shield.
The document discusses the challenges of developing offshore oil and gas fields, particularly in deep waters and remote locations. It notes that while offshore development technologies have enabled projects in up to 3,000m of water, developing fields in ultra-deep waters and frontier regions requires further technological advances. Specifically, it focuses on the need for high-capacity pipe-lay vessels using J-lay or reel-lay methods to install thick-walled pipes in these challenging environments, with each method having advantages depending on the characteristics of the specific field development project. Saipem's new flexible lay and construction vessel, the Normand Maximus, is presented as an innovative vessel designed to aid in ultra-deep water SURF installation and field
The document discusses the benefits of exercise for mental health. Regular physical activity can help reduce anxiety and depression and improve mood and cognitive functioning. Exercise causes chemical changes in the brain that may help protect against mental illness and improve symptoms.
Dr. Kenneth Dickie from Royal Centre of Plastic Surgery in Barrie, Ontario explained the use of stem cells technology in plastic surgery.
If you have any questions, please contact Dr. Kenneth Dickie at http://royalcentreofplasticsurgery.com/
Cultured skin substitutes prepared from cultured skin cells and biopolymers can reduce the need for donor skin grafts and have been shown to effectively treat excised burns, burn scars, and congenital skin lesions. Cultured skin substitutes generate skin phenotypes in the lab and restore tissue function and systemic homeostasis when implanted. Healed skin from cultured skin substitutes is smooth, soft and strong, though pigmentation may be irregular. Cultured skin substitutes close 67 times the area of donor skin compared to less than 4 times for split-thickness skin grafts, and result in similar qualitative outcomes.
This document summarizes the current state of stem cell therapy research for spinal cord injuries. It discusses the historical basis and developments in the field. It describes the two main types of cell-based therapies - regenerative cell therapy which aims to restore myelin and integrate axons, and replacement cell therapy which focuses on replacing neurons and oligodendrocytes. Various cell sources and delivery methods used in both regenerative and replacement therapies are outlined, including their advantages and limitations. The document also reviews several human clinical trials conducted to evaluate the safety and potential efficacy of different cell therapies.
This document contains multiple questions related to inflammation, immune responses, and wound healing.
1) The first question describes a case of mastitis in a new mother and asks which inflammatory cell would most likely be seen in breast fluid under the microscope.
2) The second question involves a boy who punctures his thumb with a rusty nail and develops swelling, asking which mechanism is primarily responsible for the initial swelling.
3) The third question describes a woman with pneumonia who develops a pleural effusion, asking which type of effusion is present based on characteristics provided.
4) The fourth question involves a boy with recurrent infections who has an impaired ability to generate reactive oxygen species, asking which protein
EWMA 2014 - EP457 AUTOTRANSPLANTATION OF THE ADIPOSE TISSUE DERIVED STEM CELL...EWMA
Autotransplantation of adipose tissue-derived stem cells shows promise for treating chronic wounds. In a study of 21 patients with diabetic foot or venous ulcers, 75% of venous ulcers and 76.9% of diabetic foot ulcers showed improvement after receiving autologous stem cells isolated from adipose tissue and implanted around and into the wounds. Laboratory results found that the stem cells expressed mesenchymal stem cell markers CD105, CD90, and CD73 both immediately after isolation and after one week in culture, suggesting adipose tissue is a suitable source of stem cells for cell therapy. However, the effects were fragile with single application, so further studies are needed to improve outcomes.
This document summarizes research on using autologous skeletal myoblasts (stem cells from skeletal muscle) in cellular cardiomyoplasty to repair damaged heart muscle following a myocardial infarction. Skeletal myoblasts are harvested from a muscle biopsy, expanded in culture, and then injected into the scarred heart muscle. Several clinical trials have shown promising results, with implanted cells orienting against cardiac stress and preventing thinning of the injured heart region. While further research is still needed, cellular cardiomyoplasty using autologous skeletal myoblasts shows potential as a definitive treatment for heart failure.
Further experience with the double onlay preputial flapasopahospital
This document describes the use of a double onlay preputial flap technique for repairing hypospadias in one stage. The authors reviewed 47 patients who underwent this procedure. Complications occurred in 12 patients (25%), including fistulas in 8 patients (17%), diverticula in 4 patients (9%), and other issues in fewer patients. Despite a high rate of more severe proximal hypospadias (75% penoscrotal or perineal), complication rates were comparable or lower than other techniques. The double onlay flap technique offers good cosmetic and functional results for repairing hypospadias.
Intra Cranial Stem Cell Transplant For Npc.Ppt 2Duriya Lakdawala
Finding a treatment for Niemann Pick Type C will provide hope not only to Aaditya Ravi Dasgupta and Tasneem Tankiwala in India but to many others like Addi and Cassi Hempel, Gabrielle Laverde and Peyton and Kayla Hadley in US, Husein Taher in Tanzania, South Africa, Roy Green in UK and so many more kids, teens and adults all over the world and in India that have not been diagnosed yet due to the cost and complexity of the diagnostic process. You can leave a wish for Aaditya (http://addiandcassi.com/guestbook) or for more information go to: www.HopeforAaditya.org
1) This case report describes a successful intraosseous blood transfusion in a 31-month old child presenting with hypovolemic shock and severe anemia due to epistaxis.
2) The child's hemoglobin level was 3.6 g/dl and intravenous access could not be established. An intraosseous line was placed in the proximal tibia to infuse 300 ml of fluid followed by 200 ml of blood.
3) The child's condition improved after the fluid and blood transfusion. Intraosseous access and transfusion is concluded to be a safe and effective option when intravenous access cannot be rapidly obtained in emergency situations with life-threatening blood loss or shock.
What is Stem Cell ?
History of Stem Cells ?
Stages of Embryogenesis
Blastocyst Diagram
Three types of stem cells
Differentiation of ESC
Adult Stem Cells
Bone Marrow
Umbilical cord stem cells
Factors known to affect stem cells
Niche cells activates Stem cells
Regenerative Medicine : Indian Scenario
- The speaker is a gold medalist in medicine who has received national and international awards for outstanding work in regenerative medicine.
- He was recently recognized by Ohio State University and invited for advanced training in organ development in the US.
- He has published several research papers and treated over 2000 patients using cellular medicine.
- The presentation will cover topics related to regenerative medicine including stem cell types, practical experiences, future directions in areas like organ development and 3D printing technology.
The document discusses hydatid disease, which is caused by the larval stage of the Echinococcus tapeworm. It primarily affects the liver and lungs in humans. The hydatid cyst has three layers and may contain daughter vesicles that resemble grapes. Symptoms depend on the infected organ and size of cysts. Diagnosis involves imaging tests and serology. Surgical removal is often needed but medical treatment and puncture/aspiration are also options. Prevention relies on patient education since it is a zoonotic disease transmitted between animals and humans.
This document provides information about lower segment cesarean section (LSCS). It begins with an introduction defining LSCS as a surgical procedure to deliver a fetus after 28 weeks through an incision in the abdominal and uterine walls. It then discusses the rising incidence of LSCS, common indications including previous c-section and fetal distress, and the preoperative preparations and steps of the procedure. Complications are outlined as well, including potential issues for both the mother such as hemorrhage, and fetus like prematurity. Postoperative care including monitoring, antibiotics, feeding, and expected recovery timeline are also reviewed.
This document describes the use of microdialysis catheters to monitor free flaps in reconstructive surgery at Aarhus University Hospital in Denmark. It presents two case studies where microdialysis detected issues with free flaps earlier than clinical observation alone. In the first case, microdialysis identified a problem with venous outflow in a fibula flap that was corrected surgically. In the second case, microdialysis found an issue with arterial inflow in a muscle flap that also required reoperation. The document advocates for microdialysis as an objective and sensitive monitoring method that can facilitate early intervention to save compromised free flaps.
Different Uses of Chitosan for Treating Serious Obstetric HemorrhagesClaude GINDREY
Gynecology and obstetrics research http://dx.doi.org/10.17140/GOROJ-3-129 Different Uses of Chitosan for Treating
Serious Obstetric Hemorrhages
Gabriel Carles, MD*; Clement Dabiri, MD; Ali Mchirgui, MD; El Oula Saoudi, MD; Najeh
Hcini, MD; Beatrice Seve, MD; Bruno de Matteis, MD
Department of Obstetrics
Novel Technique Combining Tissue and Mesh Repair for Umbilical Hernia in AdultsKETAN VAGHOLKAR
This document describes a new surgical technique for repairing umbilical hernias in adults that combines tissue repair with mesh reinforcement. The study evaluated 20 adult patients who underwent the novel procedure. Key aspects of the technique include reconstructing the abdominal wall midline using flaps of anterior rectus sheath, placing a mesh over the newly formed midline for reinforcement, and approximating surrounding tissues. None of the 20 patients who underwent the procedure developed a hernia recurrence in the follow-up period ranging from 10 to 18 months. The authors conclude that this combined tissue and mesh repair technique provides an effective option for umbilical hernia repair in adults.
For better view, press F5.
As we go through our lives each of us will have very different needs for our own healthcare.
Scientist's are constantly researching to make medical care treatment more personalized.
One way they are doing this is by-
Stem Cells therapy
Stem-cell therapy is the use of stem cells to treat or prevent a disease or condition.
It is also known as regenerative medicine, promotes the reparative response of diseased, dysfunctional or injured tissue using stem cells or their derivatives.
It is the next chapter of organ transplantation and uses cells instead of donor organs, which are limited in supply.
What are Stem cells?
Stem cells are called “master cells”
Stem cells are cells that are undifferentiated.
What are Stem cells?
Steam cells have the potential to become all other kinds of cells in our body.
What are Stem cells?
Types of Stem cells
How stem cell therapy works?
Disease cured by stem cell therapy.
Spinal Cord Injuries
Stem cell treatment of Diabetes mellitus type 1 & 2
Stem cell treatment of Stroke
Cancer treatment
Heart damage
Baldness
Tooth implanting
Deafness and blindness
Have stem cells already been used to treat diseases?
Ethical Consideration of Stem Cell Therapy
As the research method mainly focused on Embryonic Stem Cells, which involves taking tissue from an aborted embryo to get proper material to study. This is typically done just days after conception or between the 5th and 9th week.
Since then, researchers have moved on to more ethical study methods, such as Induced Pluripotent Stem Cells (iPS). iPS is artificially derived from a non-pluripotent cell, such as adult somatic cells.
Nowadays stem cell treatment has been spreaded throughout the world. It has also been grown commercially in developed countries.
It is thought that one day it may be the major key to treat various diseases.
Using stem cells to conduct medical research and treat disease is acceptable?
Don’t know
No
Yes
Do you approve of the extraction of stem cells from human embryos for medical research?
Don’t know
No
Yes
Stem Cell Therapy: The Future is Here! Find Out About the Clinical Trial and ...Patients Medical
Dr. Kamau Kokayi, Director of New York Stem Cell Treatment Center at Patients Medical presents research on how stem cell therapy has helped patients already.
Similar to Dr A. Kukharchuk FSC and Chronic Wounds (20)
Autism is a developmental disorder characterized by difficulties with social interaction, communication, and repetitive behaviors. It affects a person's ability to understand events around them and engage with others. While there is no cure for autism, various training methods can provide significant support. EmProCell has developed a methodology using fetal progenitor cells and fetal tissue extracts to stimulate incomplete neurogenesis and induce tolerance to transplanted neural cells. Their treatment involves transplanting fetal hematopoietic and brain cells, leading to improved social behavior. EmProCell's fetal cells are carefully produced and tested to be biologically safe and correct the triad of impairments in autism.
This document contains descriptions of various laboratory areas and rooms within a facility, listing their names, clean room classification zones, and any instruments present. The areas include entrance areas with biometric locks, changing rooms, shower rooms, corridors, sample processing rooms, cryo conservation rooms, cell culture laboratories, PCR laboratories, preparation rooms, sterilization rooms, and R&D laboratories. Zones range from unclassified to Class B, with various instruments listed for different areas like freezers, laminar airflow workstations, microscopes, centrifuges, and more.
This document summarizes stem cell anti-aging therapy and rejuvenation. It discusses how aging occurs due to a decrease in stem cells over time, and how increasing stem cell reserves can help rejuvenate the body's systems and tissues. The document proposes a stem cell therapy program using fetal stem cells ("Golden Vial") to restore youthfulness by replenishing 240 types of stem cells to renew all functional systems of the body. It also discusses quantitative measures of biological age and frailty to objectively evaluate the effects of anti-aging treatments.
Burns are caused by heat or chemicals and are classified by depth and severity. First degree burns affect only the outer layer of skin. Second degree burns penetrate deeper but heal in 1-2 weeks. Third degree burns destroy the full thickness of skin. Treatment requires specialized medical care. Rehabilitation is difficult and aims to prevent scarring and mobility loss through gradual exercise. Fetal stem cells can improve burn treatment outcomes by stimulating skin regeneration, strengthening the immune system, and reducing scarring. Four types of fetal cells are transplanted to help burns heal faster with minimal complications.
This document discusses the use of fetal stem cells in treating chronic wounds and ulcers. It presents three case studies of patients with diabetic ulcers or thromboangiitis obliterans who were treated with fetal stem cells. The treatment involved intravenous and local injections of fetal hematopoietic, neural, angioblast and neuroblast stem cells, as well as application of fetal tissue extracts. In all three cases, the treatment resulted in reduced infection, accelerated wound healing through epithelialization, and closure of the ulcers over 30-50 days. Doppler studies showed improved blood flow. This demonstrates the potential of fetal stem cells to help heal chronic wounds that previously did not respond to other therapies.
1. The document describes the facilities and procedures of Cryobank at EmProCell Clinical Research Pvt. Ltd. in Mumbai for processing and storing fetal tissue extracts and organ-specific progenitor cells.
2. EmProCell has laboratories for biotechnology, biosafety, research and development, sample processing, manufacturing, cryobanking, ELISA testing, cell culture, pathogen detection, karyotyping and hybridization, and sterilization.
3. Donor screening involves ELISA testing of pregnant women's blood for HBV, HCV, CMV, HTLV, and VDRL. Samples then undergo pathogen detection by PCR and sterility testing before being processed and cryop
This document describes various anti-aging creams and treatments produced by EmProCell Clinical Research Pvt. Ltd. that contain extracts from fetal tissue. The creams are described as containing growth factors and other components that stimulate the skin's own stem cells to promote collagen production, cell proliferation, and other anti-aging effects. The document also describes using suspensions of fetal fibroblasts and keratinocytes to treat burns and scars.
Company has been created for scientific elaboration and practical application of new biotechnological methods in human disease treatment, originated on applying fetal stem cells and tissues extract.
Company in its development reviews elaborating new biotechnological rejuvenating creams on the origin of placental extract, likewise embedding in clinic the anti-aging biotechnological complex.
The document discusses fetal progenitor cells derived from various fetal tissues that are stored in EmProCell's cryobank. These cells can be isolated from tissues of the brain, spinal cord, heart, lungs, liver, kidneys, pancreas, adrenal glands, muscles, bones, skin, and placenta from aborted fetuses between 6-20 weeks gestation. The cells have potential applications in regenerative medicine due to their ability to differentiate into specialized cell types and induce immune tolerance without risk of cancer. EmProCell is open to collaboration in using these fetal progenitor cells for conditions like cirrhosis, ischemia, wounds, and spinal injury.
More from EmProcell Clinical Research Private Limited, Mumbai, INDIA. (9)
1. Fetal Stem Cells in Chronic Wounds
& Ulcer Treatment
DOCTOR
ANDRII KUKHARCHUK
13 Apr, 2015
2. CHRONIC WOUND AND ULCER
The mainstay of therapy for
severe, limb-threatening ischemia is
either surgical or endovascular
revascularization aiming to improve
blood flow to the affected
extremity. If revascularization has
failed or is not possible, major
amputation is often necessary. This
relates to about 30% of all cases of
severe limb ischemia,
corresponding to about 100.000
major leg amputations in the EU,
and to 120.000 in the US.
Leg amputation in atherosclerotic
PAD causes an acute mortality rate
of around 30% and a grim five-year
prognosis with a survival rate of less
than 30%.
3. CHRONIC WOUND AND ULCER
Nonhealing chronic wounds are a
large and growing problem with an
incidence of 5–7 million cases per
year in the United States only.
Chronic wounds are a cause of
significant morbidity and mortality
and pose a large financial burden on
the healthcare system.
Despite the most recent advances
in wound management, up to 50%
of chronic wounds still fail to heal.
Regenerative potential of fetal
progenitor cells can help in
treatment of no healing wounds by
3 target points: activation of
angiogenesis, induction of immune
tolerance, and repair and
regeneration of the skin.
4. CHRONIC WOUND AND ULCER
Patient R., 66 yrs, M., Diabetic
Ulcer.
Ulcer not healing during 120 days
Doppler Study before treatment:
Minimal flow is seen in the
posterior tibial artery in the upper
leg and the superior margin of the
wound.
There is almost complete block of
the posterior tibial artery at the
junction of the upper and mid third
of the leg.
Flow is mildly reduced in anterior
tibial artery and dorsalis paedis
artery around the ankle and in the
foot.
Screening Photo
Before commencement of
treatment
5. CHRONIC WOUND AND ULCER
Patient R., 66 yrs, M., Diabetic Ulcer.
1st Step of Fetal Stem Cells
Treatment:
Intravenous infusion of fetal liver
hematopoietic stem cells (AC133+)
& subcutaneously injection of neural
stem cells from same fetus.
Aim:
Hematopoietic stem cells introduce
immunologic tolerance for all kinds
of allogenic stem cells from same
fetus + improve endothelial
regeneration in arteries and veins.
Neural fetal stem cells restore
innervations in zone of diabetic
neuropathy.
6. Patient R., 66 yrs, M., Diabetic
Ulcer.
2nd Step of Fetal Stem Cells
Treatment:
Local multiple injection of fetal
angioblasts and fetal
neuroblasts along obliterated
arteries
Aim
Creation new vascular
network by stimulation of
angiogenesis and
vasculogenesis.
Restore local innervations in
ischemic legs' tissue.
7. Patient R., 66 yrs, M., Diabetic
Ulcer.
3rd Step of Fetal Stem Cells
Treatment:
Local multiple injection of fetal
angioblasts and fetal neuroblasts
around the ulcer and in the
bottom of the wound.
Aim
Creation new local vascular
network by stimulation of
angiogenesis and vasculogenesis.
Restore local innervations in
ischemic tissue of wound.
8. Patient R., 66 yrs, M., Diabetic Ulcer.
4rd Step of Fetal Stem Cells
Treatment:
Application of fetal tissue extracts
(skin, muscle and umbilical cord) in
autoplasma gel.
Aim
Activation of wound epithelialization
9. Patient R., 66 yrs, M., Diabetic Ulcer.
14 days after introduction of cells. Wound on 14th day covered
ointment with recombinant epidermal growth factor.
No epithelialization
10. Patient R., 66 yrs, M., Diabetic Ulcer.
17 days after introduction of cells. Wound covered with
colloid silver and fetal skin extract gel.
No epithelialization.
11. Patient R., 66 yrs, M., Diabetic Ulcer.
20 days after introduction of cells. Size of the wound
reduced. Wound cleaned from dead tissue and covered
with colloid silver and fetal skin extract gel.
No epithelialization
12. Patient R., 66 yrs, M., Diabetic Ulcer.
24 days after introduction of cells. Wound cleaned from
dead tissue. Implantation of 4 flaps (black arrow) fetal
skin (14-15 weeks of gestation). Free surface
uncovered area by fetal skin of the wound covered
with colloid silver and placental extract
13. Patient R., 66 yrs, M., Diabetic Ulcer.
30 days after introduction of cells. 5 days after implantation
of fetal skin. Flaps of fetal skin absorbed. Epithelialization
of edges started from all sides of wound (black arrow)
under the impact of growth factors secreted from fetal
skin
14. Patient R., 66 yrs, M., Diabetic Ulcer.
31 days after introduction of cells. Repeated implantation of
fetal skin for strengthening and acceleration of wound
epithelialization.
15. Patient R., 66 yrs, M., Diabetic
Ulcer.
46 days after introduction of cells.
Repeated implantation of fetal skin
(black arrow) + ointment "EPC-
Universal":
► Amorphous Hydrogel Wound
Dressing with Colloidal silver
► Neomycin-Bacitracin
Sulphacetamide
► Fetal Hematopoietic Stem Cells
Extract
► Fetal Neural Stem Cells Extract
► Fetal Thymus Extract
► Fetal Umbilical Cord Tissue
Extract
► Fetal Spleen Extract
► Fetal Muscle & Skin Extracts
16. Patient R., 66 yrs, M., Diabetic
Ulcer.
48 days after introduction of cells.
Wound epithelialization is weak.
Offered to make patient
autologous skin transplantation
with implantation of fetal skin.
But patient refused the operation.
Autoskin
graftFetal skin
implants
17. Patient R., 66 yrs, M., Diabetic Ulcer.
49 days after introduction of cells. Wound
epithelialization is weak.
Second local multiple injections of fetal
angioblasts and fetal neuroblasts around
the wound.
Repeated implantation of fetal skin.
HbA1C:
Before treatment – 6.9%
Now – [? I HAVE NOT ANALYSES COPY!!!]
Estimated Average Glucose:
Before treatment – 151.9 mg/dl
Now – [? I HAVE NOT ANALYSES COPY!!!]
Doppler Study: Compared to previous
scan, there is extensive
neovascularisation is noted in the bed of
the wound.
Treatment continue
18. Patient R., 66 yrs, M., Diabetic Ulcer.
Before Fetal Stem Cells
Treatment
Now
(in Process of Fetal Stem Cells
Treatment)
19. Patient K., Male, Diabetic Foot.
Necrotized tissues are observed
(black arrow), canal filled with
pus (white arrow)
Wound not healing during 90
days
Doppler Study before treatment:
Generalised atherosclerotic
disease involving arteries of
right lower limb.
Extensive wall calcification is
seen in anterior tibial artery,
posterior tibial artery and
dorsalis paedis artery.
Screening Photo
Before commencement of
treatment
20. Patient K., Male, Diabetic
Foot.
Necrotized tissues are removed.
Two pus canals are cleaned
(white arrow)
21. Patient K., Male, Diabetic Foot.
Schematic representation of local wound treatment: points for
cells introduction
22. Patient K., Male, Diabetic
Foot.
Treatment:
1st Step. Intravenous infusion of
fetal liver hematopoietic stem
cells (AC133+) & subcutaneously
injection of neural stem cells
from same fetus.
2nd Step. Local multiple injection
of fetal angioblasts and fetal
neuroblasts along obliterated
arteries.
3rd Step. Local multiple injection
of fetal angioblasts and fetal
neuroblasts around the ulcer and
in the bottom of the wound
(foto).
4rd Step. Application of fetal
tissue extracts (skin, muscle and
umbilical cord) in autoplasma gel.
23. Patient K., Male, Diabetic Foot.
6 days after introduction of cells. Edges of wound constringes.
Epithelializiation of wound started in the wound edges (white arrow)
Granulation is clean covered with fibrin in some places (black arrow).
Patient walks independently. Not febrile.
24. Patient K., Male, Diabetic Foot.
10 days after introduction of cells. Clean wound canal closed
(black arrow). New skin epithelium “creeps” on the granulation
(white arrow). Patient walks independently. Not febrile.
25. Patient K., Male, Diabetic Foot.
14 days after introduction of cells. Wound clean and canal is probed
(black arrow), no pus. Epithelialization continues. Wound closed with
ointment with recombinant epidermal growth factors. Patient walks
independently. Not febrile.
26. Patient K., Male, Diabetic Foot.
17 days after introduction of cells. Clean
wound and canal probed; no pus. In the
canal and on the wound – ointment with
colloid silver and fetal skin extract.
Epithelialization continues. Patient
walks independently. Not febrile.
27. Patient K., Male, Diabetic Foot.
20 days after introduction of cells. Purulence of canal, febrile,
severe pain. Clean wound, canal probed, pus removed.
28. Patient K., Male, Diabetic Foot.
20 days after introduction of cells. In the canal and on wound –
ointment with colloid silver and fetal skin extract.
29. Patient K., Male, Diabetic Foot.
20 days after introduction of cells. Continuous epithelialization of
wound edges. Intramuscular cephalosporin 5th generation
prescribed.
30. Patient K., Male, Diabetic Foot.
24 days after introduction of cells. Again pus in the canals.
Wound is cleaned, canals probed and washed, pus removed. In
canals – antibiotic ointment. On the wound – colloid silver gel
and placental extracts. Continuous epithelialization of wound
edges.
31. Patient K., Male, Diabetic Foot.
30 days after introduction of cells. Canals probed and washed. No
pus. Continuous epithelialization of wound edges.
32. Patient K., Male, Diabetic Foot.
30 days after introduction of cells. In canals antibiotic ointment
were introduced. Wound covered with colloid silver gel and
placental extracts. Comparison foto: 3 days vs 30 days
3 days 30 days
33. Patient K., Male, Diabetic Foot.
31st day: implantation of fetal skin for strengthening and
accelerating epithelialization of wound under the impact of fetal
growth factors.
34. Patient K., Male, Diabetic Foot.
37st day: accelerating of wound epithelialization. Wound covered
with colloid silver gel and placental extracts.
35. Patient K., Male, Diabetic Foot.
38th day: pus in wound canals. Canal open and clearing.
36. Patient K., Male, Diabetic Foot.
From 38th to 44th days: Wound covered with ointment “EPC
Universal”.
37. Patient K., Male, Diabetic Foot.
46th days: Wound covered with ointment “EPC Universal”. In
canal – ointment “Levomicol”
38. Patient K., Male, Diabetic
Foot.
49th days: implantation of fetal
skin for strengthening and
accelerating epithelialization of
wound under the impact of fetal
growth factors.
HbA1C:
Before treatment – 9.2%
Now – [? I HAVE NOT THIS
ANALYSES!!!]
Doppler Study: Compared to previous
scan, there is extensive
neovascularisation is noted in the bed
of the wound.
Treatment continue
39. Patient K., Male, Diabetic Foot.
Before Fetal Stem Cells
Treatment
Now
40. Patient Ch., M., Thromboangiitis
obliterans (Berger's disease)
Wound not healing during 120 days
Doppler Study before treatment:
[??? - I HAVE NOT THIS DATA!!!
IMAGINE ONLY!!!]
Screening Photo
Before commencement of
cells' treatment
41. Patient Ch., M., Thromboangiitis
obliterans (Berger's disease)
Treatment:
1st Step. Intravenous infusion of
fetal liver hematopoietic stem
cells (AC133+) &
subcutaneously injection of
neural stem cells from same
fetus.
2nd Local multiple injection of
fetal angioblasts and fetal
neuroblasts around the ulcer
and in the bottom of the wound
(foto).
3rd Step. Application of fetal
tissue extracts (skin, muscle
and umbilical cord) in
autoplasma gel.
Local introduction of cells
42. Patient Ch., M., Thromboangiitis obliterans (Berger's
disease)
14 days after introduction of cells. Infection, pus.
Prescribed I/M Gentamycin, 80 mg 2 times per day.
Wound is covered with disinfecting cream “Hydroheal AM” with
the colloid silver
43. Patient Ch., M., Thromboangiitis obliterans (Berger's
disease)
17 days after introduction of cells. Infection, pus. Wound cleaned
from pus.
Prescribed I/M Gentamycin, 80 mg 2 times per day.
Wound is covered with disinfecting cream “Hydroheal AM” with
the colloid silver + fetal skin extract
44. Patient Ch., M., Thromboangiitis obliterans (Berger's
disease)
20 days after introduction of cells. Infection, pus.
45. Patient Ch., M., Thromboangiitis obliterans (Berger's disease)
20 days after introduction of cells. Wound cleaned.
46. Patient Ch., M., Thromboangiitis obliterans (Berger's disease)
20 days after introduction of cells. Applied antibiotic ointment on
wound: Mupirocin Oiment IP. Intramuscular cephalosporin 5th
generation.
47. Patient Ch., M., Thromboangiitis obliterans (Berger's
disease)
24 days after introduction of cells. Purulence and edema on the
2nd toe. Wound cleaned. Applied antibiotic ointment on wound:
Mupirocin Oiment IP. Intramuscular cephalosporin 5th
generation. After demarcation line appears planned for
amputation of 2nd toe.
48. Patient Ch., M., Thromboangiitis obliterans (Berger's
disease)
30 days after cells introduction. Toe amputation. Wound cleaned
from pus.
49. Patient Ch., M., Thromboangiitis
obliterans (Berger's disease)
30 after cells introduction. Antibiotic
ointment applied on wounds.
On 31st day planned to apply antibiotic
ointment + fetal extract of liver with
high content of tissue fibrinolysis
activator.
In cases of TAO in the zone of
debridement micro-thrombosis are
formed which blocks blood supply to
small portions of the wound surface. As
a result these portions in the wound
constantly necrotize and form
purulence. Activation of local
fibrinolysis in wounds under impact of
fetal liver extract can restore blood
supply to small vessels and prevent
small necrosis in the wounds.
50. Patient Ch., M., Thromboangiitis
obliterans (Berger's disease)
On 31st day was apply antibiotic ointment +
fetal extract of liver with high content of
tissue fibrinolysis activator.
51. Patient Ch., M., Thromboangiitis
obliterans (Berger's disease)
38 days: pus. On the wound - antibiotic
ointment Bactroban. Per os – Warfarin
& Trental
52. Patient Ch., M., Thromboangiitis
obliterans (Berger's disease)
40 days: wound clearing.
Granulation appear. On the wound
- antibiotic ointment Bactroban.
53. Patient Ch., M., Thromboangiitis
obliterans (Berger's disease)
41 days: wound epithelialization
starting. On the wound - ointment
"EPC Universal".
56. Patient Ch., M., Thromboangiitis obliterans (Berger's disease)
48 days: wound epithelialization continue. On the wound – ointment
"EPC Universal"
57. Patient Ch., M.,
Thromboangiitis obliterans
(Berger's disease)
49 days: fetal skin implantation.
Doppler Study:
Compared to previous study,
there is marginal improvement in
the vascularity of the wound.
Treatment continue
58. Patient Ch., M., Thromboangiitis obliterans (Berger's disease)
Before Fetal Stem Cells
Treatment
Now
(after 3.5 months)
48 days after Fetal Stem Cells
Treatment