diagnostic value of comparative analysis of the gene p53 promoter and the carboxyl part of the ubiquitin in searching the global error(s) in the syntax of the protein and DNA sequences

1. I began to research the phenomenon of degeneration of the genetic code using method of
visualization and analysis (which I bioinformatics independently developed) on the genome of
bacteriophage X174; in addition, the domain which was the issue and significant for my further
research I found out in the origin of replication of this virus (X174).
Later on, using the same bioinformatic method (visualization and analysis), I was researching
tumour suppressor genes promoters: p53, p63, p73.
In the p53 gene promoter, I observed a palindromic domain in the length of 15 nucleotides
(conditionally 17 nucleotides, with 2 frame shifts), which has the preserved palindromicity with
both frameshifts, along with the preserved specificity of the triplet in the genetic code table (also
in the frameshifts!)
This palindromic domain from the p53 gene promoter was important to me in the construction of
one completely new and unique genetic code table: in the left column of this table, there are
ranked algorithms for the matrix nucleotide sequences and in the right column there are the
amino acid groups, aligned according to the corresponding algorithms (by the left column rows).
By analyzing the amino acid groups which I obtained by constructing my table, and
simultaneously by the analysis of nucleotide triplets by the corresponding amino acid groups;
I found out that only certain amino acids, to be more precise, their nucleotide triplets are found
in this palindromic domain of p53 gene.
With very specific nucleotide triplets in the palindromic domain of p53 genes, I tried to find a
hypothetical protein domain with analogous amino acid (according to my table).
I found out the targeted domain with specific amino acids in the recognizing / ligating part of the
ubiquitin protein (Ub).
With this observation now I had the domain in the tumor suppressor gene promoter (p53) as well
as the domain in the ubiquitin molecule which are in a significant correlation through this genetic
code table
I hope that the palindromic domains found in tumour suppressor genes p53, (p63, p73), the
domain in the ubiquitin molecule, with other possible domains in the genes MDM2 and Rb ..,
along with the table I have constructed, will be a guide in determining and recognizing a
contextual incompatibility / compatibility and an error in protein synthesis; in correction of
ubiquitous-proteosome complex; in tumour suppressor genes and oncogene error along the entire
DNA and in oncoproteins.
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2. I would like to start a business and(or) scientific cooperation in different areas of scientific
research:
1.the first area is CRISPR gene editing of these palindromic domene of promoter gene p53 (and
p63,p73).
2.Synthesis of polypeptide or protein which would be prototype for endogenous synthetic error
and synthesis of corresponding monoclonal antibodies .
Next area of cooperation is the presentation of endogenous synthetic error in healthy and
malignant genome and proteinome which could be done by using advanced computional and
bioinformatic methods and by my personal genetic table, domains and methods.
Dilemmas as well as issues which I had while writing the background on Lynkedin profile still
remain unsolved even now:
how to point out the potential importance of my scientifich research (which I have been working
on for 30 years), but at the same time not to reveal the very essence and the gist of possible
discoveries.
(laconic reply would be to write a scientific article)
Neverthless, I am willing and ready for brainstorming and disccussion via correspondence in
the scientific and business contest (along with scientific and patent respect).
Any sort of correspodence is welcome.
Dr.Milan Gilić
milan.gilic1@st.t.com.hr