This study developed a methodology for cultivating Macrocystis pyrifera from spores and gametes on artificial nylon ropes installed at the sea surface. In tanks under controlled conditions, male and female gametophytes developed within 20 days, and sporophytes reached over 400 μm in length after 60 days. Ropes with 2500 μm sporophytes were transferred to intermediate cultivation in the sea, where they grew to 36 cm in 30 days. Finally, 46 specimens 30-40 cm long were suspended from surface ropes, reaching over 3 m and 7 kg after 3 months with abundant biomass but lacking stipes and holdfasts. Surface cultivation avoids herbivory encountered with sea floor cultivation.
SPONGES AND THEIR POTENTIAL USES by Ranjith Edirisingheapaari
SPONGES AND THEIR POTENTIAL USES by Ranjith Edirisinghe Presented during the Regional Workshop on Underutilized Fish and Marine Genetic Resources and their Amelioration, 10-12 July 2019, Location: Colombo, Sri Lanka
Evaluation of the Ichthyotoxicity of Dioscorea Dumetorum on the African Catfi...AJSERJournal
Dioscorea Dumetorumtubers are used in stupefying fish for easy harvesting in Akwa Ibom State, Nigeria
specifically in Annang part of the state. D. dumetorum belongs to the family of Dioscoreaceae and is commonly known
as bitter yam. This study was conducted to determine the bioactive components and evaluate the acutetoxicity of the
aqueous extract of the D. dumetorum on C. aariepinus fingerlings and anuran tadpoles. The 96hLC50 values of D.
dumetorum tuber aqueous extracts were determined in the laboratory under static bioassay conditions against C.
aariepinus fingerlings and anuran tadpoles. Range finding bioassays were conducted to get the range of concentrations
for the definitive bioassays. The range of concentrations of test media for C. aariepinus fingerlngs was 0.6-5mgl-1 while
that of anuran tadpoles was 1.6-3.8mgl-1
. The median lethal concentrations (LC50) were determined using probit
analysis. The 96hLC50 value of the test plant against exposed fingerlings was 2.153mgl-1 with lower and upper
confidence limits of 1.869 and 2.485mgl-1
respectively while that of tadpoles was 2.538mgl-1 with lower and upper
confidence limits of 2.295 and 2.771 mgl1
respectively. Unpaired t-test showed that there was no significant difference
(p>0.05) between the toxicity of the test plant to C. aariepinus fingerlings and that of anuran tadpoles. The mean water
quality parameters were within the optimal range requirement for the test species. The study indicated that D.
dumetorumexerted piscicidal property on C. aariepinus fingerlings and anuran tadpoles. The indiscriminate use of this
plant to catch fish by local fishermen should be discouraged.
Eco-Friendly Nets & Floating Row Covers Reduce Pest Infestation & Improve Tomato Yields for Smallholder Farmers in Kenya; Gardening Guidebook for Kenya www.scribd.com/doc/239851313 ~ Egerton University ~ For more information, Please see websites below:
`
Organic Edible Schoolyards & Gardening with Children =
http://scribd.com/doc/239851214 ~
`
Double Food Production from your School Garden with Organic Tech =
http://scribd.com/doc/239851079 ~
`
Free School Gardening Art Posters =
http://scribd.com/doc/239851159 ~
`
Increase Food Production with Companion Planting in your School Garden =
http://scribd.com/doc/239851159 ~
`
Healthy Foods Dramatically Improves Student Academic Success =
http://scribd.com/doc/239851348 ~
`
City Chickens for your Organic School Garden =
http://scribd.com/doc/239850440 ~
`
Huerto Ecológico, Tecnologías Sostenibles, Agricultura Organica
http://scribd.com/doc/239850233
`
Simple Square Foot Gardening for Schools - Teacher Guide =
http://scribd.com/doc/239851110
Few species are standardized and have been used as test organisms around the world in
ecotoxicological assays. In the case of sediment assessment, there are only two amphipod species
(Tiburonella viscana and Grandidierella bonnieroides) standardized protocols for toxicity test in South
Atlantic region.
SPONGES AND THEIR POTENTIAL USES by Ranjith Edirisingheapaari
SPONGES AND THEIR POTENTIAL USES by Ranjith Edirisinghe Presented during the Regional Workshop on Underutilized Fish and Marine Genetic Resources and their Amelioration, 10-12 July 2019, Location: Colombo, Sri Lanka
Evaluation of the Ichthyotoxicity of Dioscorea Dumetorum on the African Catfi...AJSERJournal
Dioscorea Dumetorumtubers are used in stupefying fish for easy harvesting in Akwa Ibom State, Nigeria
specifically in Annang part of the state. D. dumetorum belongs to the family of Dioscoreaceae and is commonly known
as bitter yam. This study was conducted to determine the bioactive components and evaluate the acutetoxicity of the
aqueous extract of the D. dumetorum on C. aariepinus fingerlings and anuran tadpoles. The 96hLC50 values of D.
dumetorum tuber aqueous extracts were determined in the laboratory under static bioassay conditions against C.
aariepinus fingerlings and anuran tadpoles. Range finding bioassays were conducted to get the range of concentrations
for the definitive bioassays. The range of concentrations of test media for C. aariepinus fingerlngs was 0.6-5mgl-1 while
that of anuran tadpoles was 1.6-3.8mgl-1
. The median lethal concentrations (LC50) were determined using probit
analysis. The 96hLC50 value of the test plant against exposed fingerlings was 2.153mgl-1 with lower and upper
confidence limits of 1.869 and 2.485mgl-1
respectively while that of tadpoles was 2.538mgl-1 with lower and upper
confidence limits of 2.295 and 2.771 mgl1
respectively. Unpaired t-test showed that there was no significant difference
(p>0.05) between the toxicity of the test plant to C. aariepinus fingerlings and that of anuran tadpoles. The mean water
quality parameters were within the optimal range requirement for the test species. The study indicated that D.
dumetorumexerted piscicidal property on C. aariepinus fingerlings and anuran tadpoles. The indiscriminate use of this
plant to catch fish by local fishermen should be discouraged.
Eco-Friendly Nets & Floating Row Covers Reduce Pest Infestation & Improve Tomato Yields for Smallholder Farmers in Kenya; Gardening Guidebook for Kenya www.scribd.com/doc/239851313 ~ Egerton University ~ For more information, Please see websites below:
`
Organic Edible Schoolyards & Gardening with Children =
http://scribd.com/doc/239851214 ~
`
Double Food Production from your School Garden with Organic Tech =
http://scribd.com/doc/239851079 ~
`
Free School Gardening Art Posters =
http://scribd.com/doc/239851159 ~
`
Increase Food Production with Companion Planting in your School Garden =
http://scribd.com/doc/239851159 ~
`
Healthy Foods Dramatically Improves Student Academic Success =
http://scribd.com/doc/239851348 ~
`
City Chickens for your Organic School Garden =
http://scribd.com/doc/239850440 ~
`
Huerto Ecológico, Tecnologías Sostenibles, Agricultura Organica
http://scribd.com/doc/239850233
`
Simple Square Foot Gardening for Schools - Teacher Guide =
http://scribd.com/doc/239851110
Few species are standardized and have been used as test organisms around the world in
ecotoxicological assays. In the case of sediment assessment, there are only two amphipod species
(Tiburonella viscana and Grandidierella bonnieroides) standardized protocols for toxicity test in South
Atlantic region.
Evaluation of the oyster farming potential of the Cintra bay (southern Morocco)Origins publication
The bay of Cintra is a marine ecosystem located in the southern Moroccan Atlantic known for its
biological richness and therefore for its high fishery productivity. This bay was chosen as a new destination for the
development of aquaculture in the southern regions of Morocco. To highlight its potential in terms of oyster
farming, a first rearing trial of triploid cupped oysters (Crassostrea gigas) as well as parallel monitoring of the
phytoplankton population and ecological parameters were undertaken. The results obtained showed that the oysters
adapt well to the conditions of the new environment where mortality was negligible, or even absent after a month
of launching. In terms of biological performance, the growth of individuals is continuous during the annual cycle
and after eight months the weight could reach 30.5 g which is a minimum weight for marketing. The AFNOR and
Lawrence and Scott indices showed a good physiological state and a better commercial quality of the oysters. The
filling rate of these oysters, with an average weight of 39.7g when lifting, is very high according to the Lawrence and
Scott index (126.3) and the average AFNOR index (around 21.8) rank them in the “Special” category.
Morphological adaptation of P. canaliculata shell to the different ecosystems...Open Access Research Paper
Different physiological, morphological, and behavioral adaptations of Pomacea canaliculata aided them in their survival to different adverse environmental conditions. Furthermore, the said adaptations can be very vital in the control and management strategies that can be employed in the areas where their population posed a threat to food security. The study employed an explorative-investigative study design for the gathering of data. Eight hundred seventy-three Golden Apple Snails from different freshwater ecosystems, namely stream, irrigational canal, and rice field were collected, cleaned, and examined. To elucidate the different adaptations of the GAS to the various ecosystems, their shell characteristics were observed, recorded, and examined. Consequently, this study found out that those shells from snails sampled in streams had bigger length, width, width of the aperture, a higher number of bands, and whorls when compared to those shells from irrigational canals and rice fields. Moreover, there was a negative correlation between pH and dissolved oxygen to the height, width, and width of the aperture. There was also a significant correlation between the temperature and width, weight, and the number of bands. It was concluded that to control and manage the population of the GAS the area should have less palatable food sources and less anthropogenic activities so that environmental parameters like high pH, lower temperature, and higher dissolved oxygen can be achieved.
Determination of Rate of Shell Accretion in Pesticide Induced Fresh Water Biv...ijtsrd
Rate of shell accretion was studied in the freshwater bivalve, Parreysia corrugata exposed to chronic treatment of pesticides; quinalphos (0.108 ppm) and thiodan (0.0708 ppm). The thickness of the accreted part was maximum at umbo, moderate in the middle and was minimum at the margin of the shell in bivalves of all three groups. Rate of shell accretion was reduced in pesticide exposed bivalves than in normal bivalves. Accreted piece of the shell found to become brittle with dull and poor nacre. Dr. Pramod P. Phirke | Dr. S.P. Zambare"Determination of Rate of Shell Accretion in Pesticide Induced Fresh Water Bivalve, Parreysia Corrugata" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-4 , June 2017, URL: http://www.ijtsrd.com/papers/ijtsrd2204.pdf http://www.ijtsrd.com/biological-science/zoology/2204/determination-of-rate-of-shell-accretion-in-pesticide-induced-fresh-water-bivalve-parreysia-corrugata/dr-pramod-p-phirke
In order to assess the Myxosporeans fauna of Cameroon fresh water fishes so
as to find the fight strategies, 655 specimens (350 Oreochromis niloticus and 305
Barbus callipterus) were sampled in Mapé river (Sanaga basin) and examined.
Standard methods were used for the sampling of fishes, conservation and microscopy.
Morphometric characteristics of the spores were used for species identification. Two
new species belonging to the genus Myxobolus Büstchli, 1882 were described namely
Myxobolus tchoumbouei n. sp in Barbus callipterus which formed cysts within various
organs (fins, skin and operculum); Myxobolus mapei n. sp parasite of kidneys and liver
in Oreochromis niloticus and Barbus callipterus. Myxobolus tchoumbouei exhibited
very long spores (19.19 x 8.89 μm), pear-shaped with rounded anterior end
sometimes flattened. Polar capsules were dissymmetrical. They measured 7.60 x 3.00
μm for the bigger and 7.06 x 2.62 μm for the smaller. Myxobolus mapei n. sp had
ellipsoidal spores (13.50 x 6.83 μm) with unequal polar capsules. The larger polar
capsule (6.44 X 2.88 μm) was about 1.5 times longer than the smaller one (4.13 X 1.61
μm) and filled half of the spiral cavity. The awareness about these parasites is useful
to find fighting strategies.
Dr. Abhijit Mitra, Associate Professor and former Head, Dept. of Marine Science, University of Calcutta (INDIA) has been active in the sphere of Oceanography since 1985. He obtained his Ph.D as NET qualified scholar in 1994. Since then he joined Calcutta Port Trust and WWF (World Wide Fund), in various capacities to carry out research programmes on environmental science, biodiversity conservation, climate change and carbon sequestration. Presently Dr. Mitra is serving as the advisor of Oceanography Division of Techno India University, Kolkata. He has to his credit about 388 scientific publications in various National and International journals, and 34 books of postgraduate standards. Dr. Mitra has successfully completed about 16 projects on biodiversity loss in fishery sector, coastal pollution, alternative livelihood, climate change and carbon sequestration. Dr. Mitra also visited as faculty member and invited speakers in several foreign Universities of Singapore, Kenya, Oman and USA. In 2008, Dr. Mitra was invited as visiting fellow at University of Massachusetts at Dartmouth, USA to deliver a series of lecture on Climate Change. Dr. Mitra also successfully guided 29 Ph.D students. Presently his domain of expertise includes environmental science, mangrove ecology, sustainable aquaculture, alternative livelihood, climate change and carbon sequestration.
Utilization of Multiple Habitat Sampling Protocol for Macroinvertebrates as Indicators of Water
Quality in Stream Ecosystem in Lawis,
Buruun, Iligan City
We compared the growth of the scallop Euuolu (Pecten) ziczuc (L.) in three situations which
potentially could be used for commercial culture, in cages maintained in suspension, in cages on the
bottom and in cages partly buried in a sediment bottom. The latter permitted the scallops to bury
themselves as in their natural habitat. Throughout the 7-month study, growth, as measured by shell
length and muscle mass, was by far superior for scallops in the partly buried cages. Possible explanations
for this are ( 1) that the scallops are stressed by enclosures which prevent them from burying
themselves and (2) that organic material at the sediment/water interface is an important food resource
and E. ziczac has better access to this when it buries itself flush with the bottom. The timing of gonadal
growth and spawning varied markedly among treatments. Some spawnings coincided with temperature
increases but others did not. Differences between scallops in suspension compared to those in bottom
treatments suggested that reproduction is as much controlled by conditions in the immediate environment
of the scallops as by large-scale environmental factors. Survival was highest for the scallops
maintained in partly buried cages.
Diversity and dispersion patterns of echinoderms in Babanlagan, Talisayan, Mi...Angelo Mark Walag
Echinoderms are fundamentally good indicators of health and status of coralline communities in marine waters. In this study, the diversity and distribution of echinoderm species were determined in Babanlagan, Talisayan, Misamis Oriental. In total, 387 individuals were collected coming from classes Echinoidea, Holothuroidea, Asteroidea, and Ophiuroidea. The majority of individuals collected were Protoreaster nodusus, which is a good indicator of reef health while the least abundant echinoderm species was Acanthaster planci. The pattern of distribution of majority of echinoderms was a clumped distribution while the other groups followed regular/uniform distribution, which may be due to limited dispersal ability and availability and available food sources. Moderate species diversity was also observed and species were rather similar in abundance, shown by the evenness index. This suggests good marine health, even under the threat of gleaning activities, active fishing, and habitat destruction. It is recommended that follow-up studies are conducted especially regarding monitoring of echinoderm species, to further assess the health of the intertidal zone in Babanlagan, Talisayan, Misamis Oriental.
Evaluation of the oyster farming potential of the Cintra bay (southern Morocco)Origins publication
The bay of Cintra is a marine ecosystem located in the southern Moroccan Atlantic known for its
biological richness and therefore for its high fishery productivity. This bay was chosen as a new destination for the
development of aquaculture in the southern regions of Morocco. To highlight its potential in terms of oyster
farming, a first rearing trial of triploid cupped oysters (Crassostrea gigas) as well as parallel monitoring of the
phytoplankton population and ecological parameters were undertaken. The results obtained showed that the oysters
adapt well to the conditions of the new environment where mortality was negligible, or even absent after a month
of launching. In terms of biological performance, the growth of individuals is continuous during the annual cycle
and after eight months the weight could reach 30.5 g which is a minimum weight for marketing. The AFNOR and
Lawrence and Scott indices showed a good physiological state and a better commercial quality of the oysters. The
filling rate of these oysters, with an average weight of 39.7g when lifting, is very high according to the Lawrence and
Scott index (126.3) and the average AFNOR index (around 21.8) rank them in the “Special” category.
Morphological adaptation of P. canaliculata shell to the different ecosystems...Open Access Research Paper
Different physiological, morphological, and behavioral adaptations of Pomacea canaliculata aided them in their survival to different adverse environmental conditions. Furthermore, the said adaptations can be very vital in the control and management strategies that can be employed in the areas where their population posed a threat to food security. The study employed an explorative-investigative study design for the gathering of data. Eight hundred seventy-three Golden Apple Snails from different freshwater ecosystems, namely stream, irrigational canal, and rice field were collected, cleaned, and examined. To elucidate the different adaptations of the GAS to the various ecosystems, their shell characteristics were observed, recorded, and examined. Consequently, this study found out that those shells from snails sampled in streams had bigger length, width, width of the aperture, a higher number of bands, and whorls when compared to those shells from irrigational canals and rice fields. Moreover, there was a negative correlation between pH and dissolved oxygen to the height, width, and width of the aperture. There was also a significant correlation between the temperature and width, weight, and the number of bands. It was concluded that to control and manage the population of the GAS the area should have less palatable food sources and less anthropogenic activities so that environmental parameters like high pH, lower temperature, and higher dissolved oxygen can be achieved.
Determination of Rate of Shell Accretion in Pesticide Induced Fresh Water Biv...ijtsrd
Rate of shell accretion was studied in the freshwater bivalve, Parreysia corrugata exposed to chronic treatment of pesticides; quinalphos (0.108 ppm) and thiodan (0.0708 ppm). The thickness of the accreted part was maximum at umbo, moderate in the middle and was minimum at the margin of the shell in bivalves of all three groups. Rate of shell accretion was reduced in pesticide exposed bivalves than in normal bivalves. Accreted piece of the shell found to become brittle with dull and poor nacre. Dr. Pramod P. Phirke | Dr. S.P. Zambare"Determination of Rate of Shell Accretion in Pesticide Induced Fresh Water Bivalve, Parreysia Corrugata" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-4 , June 2017, URL: http://www.ijtsrd.com/papers/ijtsrd2204.pdf http://www.ijtsrd.com/biological-science/zoology/2204/determination-of-rate-of-shell-accretion-in-pesticide-induced-fresh-water-bivalve-parreysia-corrugata/dr-pramod-p-phirke
In order to assess the Myxosporeans fauna of Cameroon fresh water fishes so
as to find the fight strategies, 655 specimens (350 Oreochromis niloticus and 305
Barbus callipterus) were sampled in Mapé river (Sanaga basin) and examined.
Standard methods were used for the sampling of fishes, conservation and microscopy.
Morphometric characteristics of the spores were used for species identification. Two
new species belonging to the genus Myxobolus Büstchli, 1882 were described namely
Myxobolus tchoumbouei n. sp in Barbus callipterus which formed cysts within various
organs (fins, skin and operculum); Myxobolus mapei n. sp parasite of kidneys and liver
in Oreochromis niloticus and Barbus callipterus. Myxobolus tchoumbouei exhibited
very long spores (19.19 x 8.89 μm), pear-shaped with rounded anterior end
sometimes flattened. Polar capsules were dissymmetrical. They measured 7.60 x 3.00
μm for the bigger and 7.06 x 2.62 μm for the smaller. Myxobolus mapei n. sp had
ellipsoidal spores (13.50 x 6.83 μm) with unequal polar capsules. The larger polar
capsule (6.44 X 2.88 μm) was about 1.5 times longer than the smaller one (4.13 X 1.61
μm) and filled half of the spiral cavity. The awareness about these parasites is useful
to find fighting strategies.
Dr. Abhijit Mitra, Associate Professor and former Head, Dept. of Marine Science, University of Calcutta (INDIA) has been active in the sphere of Oceanography since 1985. He obtained his Ph.D as NET qualified scholar in 1994. Since then he joined Calcutta Port Trust and WWF (World Wide Fund), in various capacities to carry out research programmes on environmental science, biodiversity conservation, climate change and carbon sequestration. Presently Dr. Mitra is serving as the advisor of Oceanography Division of Techno India University, Kolkata. He has to his credit about 388 scientific publications in various National and International journals, and 34 books of postgraduate standards. Dr. Mitra has successfully completed about 16 projects on biodiversity loss in fishery sector, coastal pollution, alternative livelihood, climate change and carbon sequestration. Dr. Mitra also visited as faculty member and invited speakers in several foreign Universities of Singapore, Kenya, Oman and USA. In 2008, Dr. Mitra was invited as visiting fellow at University of Massachusetts at Dartmouth, USA to deliver a series of lecture on Climate Change. Dr. Mitra also successfully guided 29 Ph.D students. Presently his domain of expertise includes environmental science, mangrove ecology, sustainable aquaculture, alternative livelihood, climate change and carbon sequestration.
Utilization of Multiple Habitat Sampling Protocol for Macroinvertebrates as Indicators of Water
Quality in Stream Ecosystem in Lawis,
Buruun, Iligan City
We compared the growth of the scallop Euuolu (Pecten) ziczuc (L.) in three situations which
potentially could be used for commercial culture, in cages maintained in suspension, in cages on the
bottom and in cages partly buried in a sediment bottom. The latter permitted the scallops to bury
themselves as in their natural habitat. Throughout the 7-month study, growth, as measured by shell
length and muscle mass, was by far superior for scallops in the partly buried cages. Possible explanations
for this are ( 1) that the scallops are stressed by enclosures which prevent them from burying
themselves and (2) that organic material at the sediment/water interface is an important food resource
and E. ziczac has better access to this when it buries itself flush with the bottom. The timing of gonadal
growth and spawning varied markedly among treatments. Some spawnings coincided with temperature
increases but others did not. Differences between scallops in suspension compared to those in bottom
treatments suggested that reproduction is as much controlled by conditions in the immediate environment
of the scallops as by large-scale environmental factors. Survival was highest for the scallops
maintained in partly buried cages.
Diversity and dispersion patterns of echinoderms in Babanlagan, Talisayan, Mi...Angelo Mark Walag
Echinoderms are fundamentally good indicators of health and status of coralline communities in marine waters. In this study, the diversity and distribution of echinoderm species were determined in Babanlagan, Talisayan, Misamis Oriental. In total, 387 individuals were collected coming from classes Echinoidea, Holothuroidea, Asteroidea, and Ophiuroidea. The majority of individuals collected were Protoreaster nodusus, which is a good indicator of reef health while the least abundant echinoderm species was Acanthaster planci. The pattern of distribution of majority of echinoderms was a clumped distribution while the other groups followed regular/uniform distribution, which may be due to limited dispersal ability and availability and available food sources. Moderate species diversity was also observed and species were rather similar in abundance, shown by the evenness index. This suggests good marine health, even under the threat of gleaning activities, active fishing, and habitat destruction. It is recommended that follow-up studies are conducted especially regarding monitoring of echinoderm species, to further assess the health of the intertidal zone in Babanlagan, Talisayan, Misamis Oriental.
Microplastic uptake and retention in Perna perna (L.); Tripneustes gratilla (...MACE Lab
Gemma Gerber, Thembani Mkhize, Robertson-Andersson, Gan Moodley. Presented at the ninth Scientific Symposium of the Western Indian Ocean Marine Science Association (WIOMSA) 2015.
Similar to Development of macrocystis pyrifera from spores and gametes on artificial (20)
Development of macrocystis pyrifera from spores and gametes on artificial
1. Latin American Journal of Aquatic Research 292Lat. Am. J. Aquat. Res., 40(2): 292-299, 2012
DOI: 10.3856/vol40-issue2-fulltext-5
Research Article
Development of Macrocystis pyrifera from spores and gametes on artificial
substrate. Algal production in a surface culture
Paula Celis Plá1,2
& Krisler Alveal2
1
Universidad de Barcelona, Barcelona, España
2
Departamento de Oceanografía, Universidad de Concepción, P.O. Box 160-C, Concepción, Chile
ABSTRACT. This study proposes establishing a methodology for generating algal biomass from spores and
gametes of Macrocystis pyrifera (Phaeophyta, Laminariales) on nylon ropes that are 100 m long and 3 mm in
diameter, installed on the sea surface. The algae are initially farmed in tanks with seawater of 11°-13°C, pH
8.2, salinity 28-33, radiation 75-100 μmol m-2
s-1
, aeration every 20 min, the addition of nutrients (NaNO3 and
Na3PO4), and a 12:12 photoperiod. Twenty days after beginning the cultivation, male gametophytes were 17
μm long by 2.5 μm in diameter, and female gametophytes were 10.5 μm by 7.5 μm. After 60 days of
cultivation, the elongated laminar sporophytes were 412 μm by 103 μm. After 195 days, ropes with 2500 µm
long sporophytes were installed in the sea at 1 m depth (intermediate cultivation phase), obtaining specimens
36 cm in length after 30 days. Of these specimens, 46 individuals between 30 and 40 cm in size were selected
and tied to a 15 m long guide rope that was installed on the surface by means of buoys and anchored to the
bottom. After three months, these specimens reached sizes of more than 3 m in length, with abundant laminate
biomass surface, reaching an average of 7 kg per specimen, lacking stipes, and with holdfasts of a few
centimeters. The surface technique used avoids the herbivory by crustaceans and sea urchins that occurs when
the initial developmental stages are done on the seafloor.
Keywords: Macrocystis pyrifera, spores, gametes, artificial substrate, culturing, Chile.
Desarrollo de Macrocystis pyrifera en sustrato artificial a partir de esporas
y gametos. Producción algal en cultivo de superficie
RESUMEN. Este estudio postula establecer una metodología para generar biomasa algal a partir de esporas y
gametos de Macrocystis pyrifera (Phaeophyta, Laminariales), en cuerdas de nailon de 100 m de largo y 3 mm
de diámetro, instaladas en la superficie del mar y cultivadas inicialmente en estanques con agua de mar a 11º-
13ºC, pH 8,2; 28-33 de salinidad, 75-100 μmol m-2
s-1
de radiación y aireación cada 20 min, con adición de
nutrientes (NaNO3 y Na3PO4) y fotoperiodo 12:12. A los 20 días de iniciado el cultivo se obtuvo gametofitos
masculinos de 17 μm de largo por 2,5 μm de diámetro y gametofitos femeninos de 10,5 μm por 7,5 μm. A 60
días de cultivo, esporofitos laminares alargados de 412 μm por 103 μm. A los 195 días las cuerdas con
esporofitos de 2500 µm, fueron instaladas en el mar a 1 m de profundidad (fase de cultivo intermedia),
obteniéndose ejemplares de 36 cm de largo después de 30 días. De estos ejemplares se seleccionó 46
individuos de 30 a 40 cm de talla, los cuales fueron atados a una cuerda guía de 15 m de largo, instalada en la
superficie mediante boyas y anclada al fondo. A los tres meses, estos ejemplares alcanzaron tallas sobre 3 m
de longitud con abundante biomasa laminar en superficie logrando 7 kg promedio por ejemplar, ausencia de
estipes y generación de discos de fijación de pocos centímetros. La técnica utilizada cultivando en superficie
evita la herbivoría por crustáceos y erizos que ocurre cuando se efectúan cultivos de fondo en las etapas de
desarrollo inicial.
Palabras clave: Macrocystis pyrifera, esporas, gametos, sustrato artificial, cultivo, Chile.
___________________
Corresponding author: Paula Celis Plá (paulacelispla@gmail.com)
INTRODUCTION
Macrocystis pyrifera (L) C. Agardh is distributed
along the coasts of North and South America (Coyer
et al., 2001). In Chile, M. pyrifera (L) C. Agardh is
present along the entire continental Chilean coast
(17º29'-56º32'S) (Alveal et al., 1982; Ramírez &
Santelices, 1991) and has been exploited along the
2. Development of Macrocystis pyrifera from spores293
northern and central zones of Chile. It is an integral
part of both national and international demand for
brown seaweeds, a demand that is not satisfied by
algal species like Laminaria, Durvillaea, Ecklonia,
Sargassum, Lessonia (Alveal et al., 1990). M. pyrifera
is used in Chile as a foodstuff in the cultivation of
abalone (Haliotis spp.) (Fundación Chile, 2001), and
exported, ground up, for the extraction of alginic acid.
After work by North (1971) regarding the biology
of Macrocystis in California, Braud et al. (1974)
carried out cultures of this species inoculating ropes
with spores, which resulted in successful transplants,
of plants reaching over 15 m in length in the coasts of
France. Studies carried out by Candia et al. (1979),
and Alveal et al. (1982), only achieved the
development of the initial sporophyte stages with
specimens from the area of Concepción (36º47’S,
72º58’W) and specimens from Beagle Channel (55ºS,
66ºW), Chile.
Several studies about marine ecological interrela-
tionships have identified M. pyrifera as an important
refuge environment from herbivory, for nurseries of
invertebrates and fish (Macchiavello et al., 2010).
These studies were conducted by various national
researchers in different parts of continental Chile
(Santelices et al., 1981; Moreno & Jaramillo, 1983;
Ojeda & Santelices, 1984; Santelices & Ojeda, 1984a,
1984b; Dayton, 1985; Vásquez & Santelices, 1990;
Lancellotti & Vásquez, 1999). Research regarding the
social and economic importance of M. pyrifera has
been carried out in Chile as well (Alveal, 1995;
Vásquez, 2008; Westermeier et al., 2011). Experi-
mental and ecological works, as well as an
understanding of the reproductive strategies of this
species, have permitted the determination of
population dynamic aspects of M. pyrifera in southern
Chile (Buschmann et al., 2006).
Furthermore, in previous studies it has been
postulated that a correctly controlled culture of this
species facilitates the building of biological barriers to
attenuate wave impact along the shore (Westermeier
& Möller, 1990). It has also been established that this
algae can be useful in the reconstruction of zones that
have been destroyed by anthropic activities (Celis &
Alveal, 2003). Moreover, M. pyrifera is used as part of
the diet of cultured mollusks, increasing its econo-
mical and commercial value (Stuart & Brown, 1994).
The central aim of this study was to generate a
culturing methodology for M. pyrifera utilizing the
implantation of spores and gametes on artificial
substrates, through the manipulation of microscopic
gametophytes and sporophytes in controlled hatchery
conditions.
MATERIALS AND METHODS
Collection of fertile material
Algae were collected from natural populations found
in Coliumo (36º32’S, 72º58’W) and in the area of
Concepción Bay, Chile (36º39’S, 73º04’W). The
reproductive specimens were transported to the
laboratory in plastic containers, maintained at 12°C,
and the fertile sporophylls were gently cleaned with
brushes under flowing tap water before sporulation.
Inoculation and incubation process in tanks
The fertile sporophylls were placed on PVC frames,
50 x 50 cm square, with 100 m of nylon rope, 3 mm in
diameter, and installed in 2000 L plastic containers
with filtered seawater (0.45 μm). The fertile material
was removed once the release of the spores occurred.
Meanwhile, the frames with the inoculated substrate
was moved to 350 L containers with aeration
provided.
During the incubation and initial growing period,
the culture was maintained at pH between 8.0 and 8.2,
light intensity between 75-100 μmol m-2
s-1
, salinity of
the seawater between 28-33, temperature between 11-
13°C, with addition of aeration for 20 min every 2 h
(Fig. 1) and a 12:12 photoperiod.
The water was changed three times a week (Merrill
& Gillingham, 1991), with seawater enriched in the
laboratory with agricultural nutrients including NaNO3
at a concentration of 0.06 g L-1
and Na3PO4 at a
concentration of 0.01 g L-1
(Alveal et al., 1997; Celis
& Alveal, 2003). Growth was measures with an ocular
caliper on a Zeiss Oberkochen microscope, obtaining
photographs of the initial gametophytes and sporo-
phytes with a Zeiss camera. With this species, the
study enabied the generation of productive stages,
with the development of biomass in the ocean surface.
Intermediate phase
Upon finalization of the laboratory cultivation in an
intermediate culture medium (after 100-200 days) the
substrate (nylon rope) was moved to the ocean for 30
days, from November to December. The frame was
maintained at a depth of 1 m.
Suspended culture
At the end of the intermediate phase, 46 samples, with
an average length of 36 cm, were selected and
attached to ropes 2 cm in diameter and 15 m in length.
This system was maintained on the sea surface with
flotation devices anchored to the sea floor, and
identified with a number to follow the unique
development of each individual and to understand the
3. Latin American Journal of Aquatic Research 294
Figure 1. Culture system in tanks. a) Frame with nylon ropes, b) substrate in incubation and growth of the initial
microthalli.
Figura 1. Sistema de cultivo en estanques. a) Bastidor con cuerda de nylon, b) sustrato en incubación y crecimiento
inicial de microtalos.
developmental processes, both similar and different, to
natural growth patterns. Inoculation in tanks facilitates
the adhesion and development of more than 10
sporophytes per linear cm of rope and the latter
possibility of obtaining samples for comparisons of
morphological development. The sporophytes were
maintained in the sea for three months.
RESULTS
Inoculation and incubation in the hatchery phase
The release of the spores occurred 2 h after the start of
the experiment. This was followed by their settlement
on the nylon rope and the commencement of
gametophyte development between 24 and 72 h. The
formation of the lateral tube reached an average of
43.4 μm and the two-celled structure reached an
average of 63 μm in length at 100 h. After five days
the gametophytes consisted of 4 to 6 cells. The female
gametophytes, 7.5 μm in diameter, appeared strongly
pigmented while the male gametophytes were less
pigmented and 2.5 μm in diameter. The gametophytes
measured an average of 62.6 μm in length at nine days
of development, 105 μm at 13 days, and 132 μm at 20
days (Fig. 2).
During this stage and after 20 days of culture, the
male gametophytes developed antheridia, while
fertilized female cells developed into zygotes that
divided into 2 cells (an upper and lower one). The first
division is always parallel to the substrate. The
sporophytes develop on a hyaline stalk that
corresponds to the area of the embryo-spore of the
female gametophyte. At first, it had a columnar and
oval shape, strongly pigmented, with two rows of cells
along the same plane in clear apical cell (Fig. 3).
The sporophytes were 133 μm in length at 24 days.
They gradually acquired a laminar shape after 39 days
achieving 287.9 μm in length, with a wide apical
section containing 4 to 5 long thin and hyaline
rhizoids. At 52 days they had reached an average
length of 535 μm.
After 98 days of hatchery culture the size of the
sporophytes was on the average 552.42 μm in length,
with rhizoids fixed to the nylon substrate, which
favors good growth. At 148 days, they reached an
average length of 1436 μm, and at 195 days, the end
of the laboratory phase, the sporophytes measured
2750 μm, with fronds that tended to be flattened and
with a clear development of the holdfast disk.
Likewise, lamina, stipes and initial holdfasts were
identifiable (Figs. 4 and 5). The growth of this stage is
fast and the formation of the holdfast system is
notable, obviously seeking a strong adherence to the
substrate, as well as the generation of tissue to form
the assembly of the initial lamina upon which the
definitive specimen will be structured. In the hatchery
at 6.5 months the specimens showed an average length
of 0.275 cm (Fig. 5).
Sea culture stage (intermediate phase)
The inoculated ropes were moved to the sea, initiating
the intermediate phase, until they reached an average
length of 36 cm, growing between 16 and 122 cm
during the one month of cultivation. After this phase
46 specimens were selected, on the average, between
30 and 40 cm in size, and fixed to a rope 15 m in
length, installed on the water surface.
Suspended culture
The development of the suspended adult sporophytes
began at 7.5 months, with an initial wet weight of 90.4
g and size of 36 cm. After 17 days they reached 52.3
cm and 88.1 cm in average length by 33 days of
development. In the suspended culture phase, the
developing stipes formed rhizoids in the upper
4. Development of Macrocystis pyrifera from spores295
Figure 2. Incubation phase in hatchery. a) Male gametophytes, b) female gametophytes, c) female and male
gametophytes on nylon substrate (20 days).
Figura 2. Fase de incubación en hatchery. a) Gametofitos masculino, b) gametofito femenino, c) gametofitos femeninos y
masculinos sobre sustrato de nylon (20 días).
Figure 3. a) Initial development of the sporophytes over embryospores, b) female gametophyte strongly pigmented
(30 days).
Figura 3. a) Desarrollo inicial de los esporofitos sobre las embrioesporas, b) el gametofito femenino fuertemente
pigmentado (30 días).
Figure 4. Growth of sporophytes during 195 days
cultured in hatchery.
Figura 4. Crecimiento de esporofitos durante 195 días
cultivados en hatchery.
sections reducing their size from 1.8 to 0.8 cm at the
end of the experiment (Figs. 6 and 7).
At 87 days of cultivation the specimens reached an
average length of 2.28 m with a maximum of 3 m. At
the end of the study, the majority of the specimens had
a holdfasts 6 cm in diameter, 3 m in length and a
biomass of 2.7 kg; with a maximum of 7 kg wet
weight per specimen (Figs. 8 and 9).
The results howed values of 91 g at the beginning
of this stage with a 500% increase after 90 days, with
average values of between 2.7 kg (Figs. 9 and 10).
DISCUSSION
These experiments utilized basic knowledge of the life
cycle previously identified by North (1971), Alveal et
al. (1982), and Braud et al. (1974). These authors
achieved significant success transplanting the initial
5. Latin American Journal of Aquatic Research 296
Figure 5. a) Initial sporophytes, b) the development of the holdfast disk (195 days).
Figura 5. a) Esporofitos iniciales, b) desarrollo del disco de fijación (195 días).
Figure 6. Variation in stipe size during the suspended
culture phase.
Figura 6. Variación del tamaño del estipe durante la fase
de cultivo suspendido.
stages of M. pyrifera in France, and subsequent
development in the sea resulted in plants of more than
15 m in length.
The culturing of M. pyrifera done by different
authors in Chile followed the methodology of Merril
& Guillighan (1991), who inoculated ropes with
gametophytes and sporophytes of Nereocystis which
were then developed in the sea. However, prior to this,
Braud et al. (1974) and Gutiérrez et al. (2006), using a
similar method, also achieved the development of
adult specimens in the sea.
Celis & Alveal (2003), and the present study,
installed microscopic phases of M. pyrifera on ropes
and grew them in 2000 L tanks with commercial
Figure 7. Sporophytes on the ropes after the inter phase
stage (30 days on the sea).
Figura 7. Esporofitos en cuerdas después de la etapa de
interfase (30 días en el mar).
nutrients and controlling temperature, pH, salinity,
water flow and illumination. In this way, the
production of laminar biomass in the sea was made
significant by avoiding the generation of stipes, and
thereby, demonstrating a system clearly distinct from
that used by Westermeier et al. (2007, 2010, 2011),
Braud et al. (1974), and Gutiérrez et al. (2006), who
used smaller capacity containers in the laboratory and
with the development of stipes in the sea. Among the
species of the genera Macrocystis, Durvillaea, and
Lessonia in continental Chile, M. pyrifera and
Lessonia trabeculata, have given positive results
during the culturing process (Edding & Tala, 2003;
Westermeier et al., 2006, 2011).
The massive use of inoculated ropes combining the
manual process of fixing the primary ropes as
6. Development of Macrocystis pyrifera from spores297
Figure 8. Growth of sporophytes in the suspended
culture (90 days).
Figura 8. Crecimiento de los esporofitos en cultivo
suspendido (90 días).
indicated by Gutiérrez et al. (2006) and Westermeier
et al. (2007), anchoring and harvesting, without any
doubt, is labor intensive. With this system of
cultivation, there is direct development of the lamina
on the surface, which would undoubtedly facilitate the
harvest and the possibility of new generations from
the post-harvest remnants and natural re-inoculations
on the artificial substrates. The surface crops system
prevents the processes of pruning by decapod
crustaceans which remove the initial stipes when the
crops are installed at the bottom of the sea.
This means of culturing demonstrated an outs-
tanding increase in the biomass of the lamina in
systems placed on the sea surface and a decrease in the
size of the stipe during the suspended culture,
generating, as a result, energy conservation for the
plants in order to produce greater lamina biomass as
well as the formation of holdfasts of only 6 cm in
diameter in contrast to the size achieved by
Westermeier et al. (2011) using crossed specimens of
M. pyrifera and M. integrifolia (M. pyrifera). This
differed significantly from the results obtained by
Gutiérrez et al. (2006) where the weight of the stipes
was equivalent to that of the sample lamina cultivated
at 2 m depth during seven months which included
swells and waves that caused important losses to thalli
30-50 cm in length.
Inoculation and growth trials of M. pyrifera on
hard substrates or submerged rocky platforms have not
been conducted. However, in tanks, the handling of
the early stages can be controlled and verified through
microscopic observations (Westermeier & Möller,
1990); Gutiérrez et al. (2006) as well as Macchiavello
Figure 9. Biomass variations during 90 days of culture at
the sea.
Figura 9. Variación de biomasa durante 90 días de
cultivo en el mar.
Figure 10. Adult sporophythes at the end of the study.
Figura 10. Esporofitos adultos al final del estudio.
et al. (2010) obtained good yields: 13-22 kg m-1
and
24 kg m-1
, measured in linear meters of rope
respectively, which were close to the 21 kg m-1
achieved by Celis & Alveal (2003). The increase in
size to 175 cm at 120-150 days, obtained by
Macchiavello et al. (2010) on vertical lines in the sea,
shows that an annual harvest is possible using the
tested methods, even though the size reached was
much less that the 14 m reported by Westermeier et al.
(2006). Macchiavello et al. (2010) projected an annual
production of more than 100 ton per hectare per year.
The method used, in this study, to manage the
developmental conditions of the initial gametophytes
and sporophytes of M. pyrifera contrasts with those
7. Latin American Journal of Aquatic Research 298
utilized by Gutiérrez et al. (2006) who selected the
spores and later inoculated the substrates by placing
them in a cylinder. This study took advantage of the
ability to control factors such as salinity, pH, nutrients,
water flow and illumination, made possible by the use
of 2000 L tanks with sea water filtered through 0.45
µm to permit the inoculation, development and
subsequent mass production of M. pyrifera directly on
ropes at the surface. The development of the first
stages of the M. integrifolia and M. pyrifera forms in
barrels by Westermeier et al. (2007) proved to be
successful. Likewise, Macchiavello et al. (2010) had
success using the addition of GeO2 to limit the
presence of diatoms, one of the things that most
strongly interfere during tank culturing. The
inoculation in large volume tanks limits the usefulness
of expensive reagents like germanium dioxide (GeO2)
and laboratory nutrients. For this reason, the use of
agricultural nutrients was given high priority in this
study.
One of the critical aspects of the initial phase of
culturing in tanks is taking into account the constant
care needed to maintain the pH. This should fluctuate,
ideally, between 8-8.2. Likewise, the development of
opportunists like algae from the Chlorophyta group or
diatoms, and occasionally tunicates and bivalves,
especially in the final stages of development, should
be avoided. Culturing on the surface helps avoiding
the effects of interfering species. For this reason, care
and cleaning processes should be done on a permanent
basis especially when the thalli of M. pyrifera are
small (<2 cm) (Celis & Alveal, 2003). Increases in
fouling influence the floating ability of the substrates
in the sea. However, in this culture it was detected that
shading from plants with large, abundant fronds
impacted their neighbors by impeding them to grow
with a homogeneous rate.
None of the previous studies on culturing of brown
algae in Chile discussed the more productive systems
of commercial culture, especially in terms of the
installation in the sea. The substrates should be
manageable in the laboratory, onboard boats, and in
the sea.
For the model organism Macrocystis, the type of
massive culture proposed will have to answer several
concerns. With this alga the culture systems utilized
are not natural like those used with Gracilaria, which
have proven to be successful. For Macrocystis the
inoculation system, the use of ropes, the anchoring
system, adjustments and the harvesting system, the
repositioning of biomass, the reuse of substrates,
culturing on the sea floor or on the surface, all of them
give an idea of the complexity of the total system. In
addition there, is the labor and the time necessary to
recover the investment which can only be correctly
determined through pilot and pre-pilot investigations.
ACKNOWLEDGEMENTS
We acknowledge financial support from a scholarship
from the Regional Government of Bío-Bío. Thanks to
the anonymous reviewers for their valuable
suggestions. We also thank Dr. A.D. Mann for the
English translation.
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