From Jisc's campus network engineering for data-intensive science workshop on 19 October 2016.
https://www.jisc.ac.uk/events/campus-network-engineering-for-data-intensive-science-workshop-19-oct-2016
Accumulo Summit 2014: Addressing big data challenges through innovative archi...Accumulo Summit
The ability to collect and analyze large amounts of data is a growing problem within the scientific community. The growing gap between data and users calls for innovative tools that address the challenges faced by big data volume, velocity and variety. The Massachusetts Institute of Technology, Lincoln Laboratory (MIT LL) is not immune to these challenges and has developed a set of tools that address many of these challenges.
Big data volume stresses the storage, memory, and compute capacity of a computing system and requires access to a computing cloud. Choosing the right cloud is problem specific. Currently, there are four multi-billion dollar ecosystems that dominate the cloud computing environment: enterprise clouds, big data clouds, SQL database clouds, and supercomputing clouds. Each cloud ecosystem has its own hardware, software, conferences, and business markets. The broad nature of business big data challenges make it unlikely that one cloud ecosystem can meet its needs and solutions are likely to require the tools and techniques from more than one cloud ecosystem. The MIT Supercloud was developed to address this challenge. To our knowledge, the MIT SuperCloud is the only deployed cloud system that allows all four ecosystems to co-exist without sacrificing performance or functionality.
The velocity of big data velocity stresses the rate at which data can be absorbed and meaningful answers produced. Led by the NSA, a Common Big Data Architecture (CBDA) was developed for the U.S. government based on the Google Big Table NoSQL approach and is now in wide use. MIT/LL played a leading role in developing the CBDA and is a leader in adapting the CBDA to a variety of big data challenges.
Big data variety may present the largest challenge and greatest opportunities. The promise of big data is the ability to correlate diverse and heterogeneous data to form new insights. The centerpiece of the CBDA is the NSA developed Apache Accumulo database (capable of millions of entries/second) and the MIT/LL developed D4M schema. These technologies allow vast quantities of highly diverse data (text, computer logs, and social media data, etc.) to be automatically ingested into a common schema that enables rapid query and correlation of every element.
The talk will concentrate on how we utilize the aforementioned technologies in our mission to apply advanced technology to problems of national security.
Readying the campus for the internet of things (io t) - Networkshop44Jisc
Avaya, alongside Leeds Beckett University, will look at a better way to build the smart campus and ready it for the agility and security demands placed upon it by the IoT. Can vastly reducing the number of protocols required to build a campus network lead to reduction in complexity and simultaneously increase security, agility, resilience and performance?
Stream Data into the Cloud with Raspberry Pi and Windows 10 IoT CoreMike Branstein
KiZAN will bring 25 Raspberry Pi starter kits that run Windows 10 IoT Core. This will enable participants to build a really compelling IoT/Azure/Power BI story in a single day! Interet of Things (IoT) Raspberry Pi starter kit
We’ll start off the day with an introduction to IoT and build IoT devices (hands on). Next, we’ll build a simple temperature sensor, collecting ambient temperature readings, and stream the data to an Azure IoT Hub.
Once the data is in Azure, we’ll analyze it with Azure Stream Analytics, and ship it to an Azure SQL Database.
Finally, we’ll report on the data and build dashboards of our temperature readings using Power BI.
This presentation gives the introduction about cloud storage & its limitations, Introduction to world's first underwater data center which is Microsoft's Underwater Cloud Storage project called "Natick" with its deployment.
From Jisc's campus network engineering for data-intensive science workshop on 19 October 2016.
https://www.jisc.ac.uk/events/campus-network-engineering-for-data-intensive-science-workshop-19-oct-2016
Accumulo Summit 2014: Addressing big data challenges through innovative archi...Accumulo Summit
The ability to collect and analyze large amounts of data is a growing problem within the scientific community. The growing gap between data and users calls for innovative tools that address the challenges faced by big data volume, velocity and variety. The Massachusetts Institute of Technology, Lincoln Laboratory (MIT LL) is not immune to these challenges and has developed a set of tools that address many of these challenges.
Big data volume stresses the storage, memory, and compute capacity of a computing system and requires access to a computing cloud. Choosing the right cloud is problem specific. Currently, there are four multi-billion dollar ecosystems that dominate the cloud computing environment: enterprise clouds, big data clouds, SQL database clouds, and supercomputing clouds. Each cloud ecosystem has its own hardware, software, conferences, and business markets. The broad nature of business big data challenges make it unlikely that one cloud ecosystem can meet its needs and solutions are likely to require the tools and techniques from more than one cloud ecosystem. The MIT Supercloud was developed to address this challenge. To our knowledge, the MIT SuperCloud is the only deployed cloud system that allows all four ecosystems to co-exist without sacrificing performance or functionality.
The velocity of big data velocity stresses the rate at which data can be absorbed and meaningful answers produced. Led by the NSA, a Common Big Data Architecture (CBDA) was developed for the U.S. government based on the Google Big Table NoSQL approach and is now in wide use. MIT/LL played a leading role in developing the CBDA and is a leader in adapting the CBDA to a variety of big data challenges.
Big data variety may present the largest challenge and greatest opportunities. The promise of big data is the ability to correlate diverse and heterogeneous data to form new insights. The centerpiece of the CBDA is the NSA developed Apache Accumulo database (capable of millions of entries/second) and the MIT/LL developed D4M schema. These technologies allow vast quantities of highly diverse data (text, computer logs, and social media data, etc.) to be automatically ingested into a common schema that enables rapid query and correlation of every element.
The talk will concentrate on how we utilize the aforementioned technologies in our mission to apply advanced technology to problems of national security.
Readying the campus for the internet of things (io t) - Networkshop44Jisc
Avaya, alongside Leeds Beckett University, will look at a better way to build the smart campus and ready it for the agility and security demands placed upon it by the IoT. Can vastly reducing the number of protocols required to build a campus network lead to reduction in complexity and simultaneously increase security, agility, resilience and performance?
Stream Data into the Cloud with Raspberry Pi and Windows 10 IoT CoreMike Branstein
KiZAN will bring 25 Raspberry Pi starter kits that run Windows 10 IoT Core. This will enable participants to build a really compelling IoT/Azure/Power BI story in a single day! Interet of Things (IoT) Raspberry Pi starter kit
We’ll start off the day with an introduction to IoT and build IoT devices (hands on). Next, we’ll build a simple temperature sensor, collecting ambient temperature readings, and stream the data to an Azure IoT Hub.
Once the data is in Azure, we’ll analyze it with Azure Stream Analytics, and ship it to an Azure SQL Database.
Finally, we’ll report on the data and build dashboards of our temperature readings using Power BI.
This presentation gives the introduction about cloud storage & its limitations, Introduction to world's first underwater data center which is Microsoft's Underwater Cloud Storage project called "Natick" with its deployment.
KiZAN will bring 25 Raspberry Pi starter kits that run Windows 10 IoT Core. This will enable participants to build a really compelling IoT/Azure/Power BI story in a single day! Interet of Things (IoT) Raspberry Pi starter kit
We’ll start off the day with an introduction to IoT and build IoT devices (hands on). Next, we’ll build a simple temperature sensor, collecting ambient temperature readings, and stream the data to an Azure IoT Hub.
Once the data is in Azure, we’ll analyze it with Azure Stream Analytics, and ship it to an Azure SQL Database.
Finally, we’ll report on the data and build dashboards of our temperature readings using Power BI.
KiZAN will bring 25 Raspberry Pi starter kits that run Windows 10 IoT Core. This will enable participants to build a really compelling IoT/Azure/Power BI story in a single day! Interet of Things (IoT) Raspberry Pi starter kit
We’ll start off the day with an introduction to IoT and build IoT devices (hands on). Next, we’ll build a simple temperature sensor, collecting ambient temperature readings, and stream the data to an Azure IoT Hub.
Once the data is in Azure, we’ll analyze it with Azure Stream Analytics, and ship it to an Azure SQL Database.
Finally, we’ll report on the data and build dashboards of our temperature readings using Power BI.
Speaking at John Carrol University on the Internet of ThingsJustin Grammens
I was honored to have been invited to speak at John Carroll University on “The Internet of Things - Making the Physical World Smarter.” It was an extremely fun and engaging audience and I enjoyed every minute of the presentation. I hope you enjoy it as well and please don't hesitate to reach out with any questions.
KiZAN will bring 25 Raspberry Pi starter kits that run Windows 10 IoT Core. This will enable participants to build a really compelling IoT/Azure/Power BI story in a single day! Interet of Things (IoT) Raspberry Pi starter kit
We’ll start off the day with an introduction to IoT and build IoT devices (hands on). Next, we’ll build a simple temperature sensor, collecting ambient temperature readings, and stream the data to an Azure IoT Hub.
Once the data is in Azure, we’ll analyze it with Azure Stream Analytics, and ship it to an Azure SQL Database.
Finally, we’ll report on the data and build dashboards of our temperature readings using Power BI.
KiZAN will bring 25 Raspberry Pi starter kits that run Windows 10 IoT Core. This will enable participants to build a really compelling IoT/Azure/Power BI story in a single day! Interet of Things (IoT) Raspberry Pi starter kit
We’ll start off the day with an introduction to IoT and build IoT devices (hands on). Next, we’ll build a simple temperature sensor, collecting ambient temperature readings, and stream the data to an Azure IoT Hub.
Once the data is in Azure, we’ll analyze it with Azure Stream Analytics, and ship it to an Azure SQL Database.
Finally, we’ll report on the data and build dashboards of our temperature readings using Power BI.
KiZAN will bring 25 Raspberry Pi starter kits that run Windows 10 IoT Core. This will enable participants to build a really compelling IoT/Azure/Power BI story in a single day! Interet of Things (IoT) Raspberry Pi starter kit
We’ll start off the day with an introduction to IoT and build IoT devices (hands on). Next, we’ll build a simple temperature sensor, collecting ambient temperature readings, and stream the data to an Azure IoT Hub.
Once the data is in Azure, we’ll analyze it with Azure Stream Analytics, and ship it to an Azure SQL Database.
Finally, we’ll report on the data and build dashboards of our temperature readings using Power BI.
Cyberinfrastructure and its Role in ScienceCameron Kiddle
This presentation examines some of the challenges scientists face and describes various cyberinfrastructure technologies that help address these challenges. Example projects employing cyberinfrastructure technologies that we have worked on at the Grid Research Centre, including the GeoChronos project, are also presented. This presentation was given at the IAI International Wireless Sensor Networks Summer School held at the University of Alberta on July 6th, 2009.
These are my slides for the 5 minute overview talk I gave during a recent workshop at the European Commission in Brussels, on the topic of "Big Data Skills in Europe".
Project Natick-Datacenters under sea, a project by microsoft.
Project Natick reflects Microsoft’s ongoing quest for cloud datacenter solutions that offer rapid provisioning, lower costs, high responsiveness, and are more environmentally sustainable.
The tutorial on the Web of Things discusses possible solutions to build the entire vertical system by identifying the relevant components, illustrating their functionality and integration, and showing the examples of existing tools and
systems. First, the tutorial covers architectural aspects and discusses the levels of abstraction for integrating the “things” into the Web. Next, the tutorial focuses on semantic technologies and analytic methods for leveraging services and applications on top of the “things”. State of the art technology and tools are showed through live demos. The tutorial concludes with a brief review of existing projects and an outline of research directions and challenges.
Common Design Elements for Data Movement Eli DartEd Dodds
Eli Dart, Network Engineer ESnet Science Engagement Lawrence Berkeley National Laboratory Cosmology CrossConnects Workshop Berkeley, CA February 11, 2015
Michael Sullivan, M.D. Associate Director, Health Sciences, Internet2
AAMC 2013 Information Technology in Academic Medicine Conference Vancouver CA June 5-7, 2013
This was a 30 min talk intended as one of the opening/overview presentations before a full-day deep dive into ScienceDMZ design patterns and architectures.
Direct downloads are not enabled. Contact me directly (chris@bioteam.net) if you for some odd reason want a copy of this slide deck!
KiZAN will bring 25 Raspberry Pi starter kits that run Windows 10 IoT Core. This will enable participants to build a really compelling IoT/Azure/Power BI story in a single day! Interet of Things (IoT) Raspberry Pi starter kit
We’ll start off the day with an introduction to IoT and build IoT devices (hands on). Next, we’ll build a simple temperature sensor, collecting ambient temperature readings, and stream the data to an Azure IoT Hub.
Once the data is in Azure, we’ll analyze it with Azure Stream Analytics, and ship it to an Azure SQL Database.
Finally, we’ll report on the data and build dashboards of our temperature readings using Power BI.
KiZAN will bring 25 Raspberry Pi starter kits that run Windows 10 IoT Core. This will enable participants to build a really compelling IoT/Azure/Power BI story in a single day! Interet of Things (IoT) Raspberry Pi starter kit
We’ll start off the day with an introduction to IoT and build IoT devices (hands on). Next, we’ll build a simple temperature sensor, collecting ambient temperature readings, and stream the data to an Azure IoT Hub.
Once the data is in Azure, we’ll analyze it with Azure Stream Analytics, and ship it to an Azure SQL Database.
Finally, we’ll report on the data and build dashboards of our temperature readings using Power BI.
Speaking at John Carrol University on the Internet of ThingsJustin Grammens
I was honored to have been invited to speak at John Carroll University on “The Internet of Things - Making the Physical World Smarter.” It was an extremely fun and engaging audience and I enjoyed every minute of the presentation. I hope you enjoy it as well and please don't hesitate to reach out with any questions.
KiZAN will bring 25 Raspberry Pi starter kits that run Windows 10 IoT Core. This will enable participants to build a really compelling IoT/Azure/Power BI story in a single day! Interet of Things (IoT) Raspberry Pi starter kit
We’ll start off the day with an introduction to IoT and build IoT devices (hands on). Next, we’ll build a simple temperature sensor, collecting ambient temperature readings, and stream the data to an Azure IoT Hub.
Once the data is in Azure, we’ll analyze it with Azure Stream Analytics, and ship it to an Azure SQL Database.
Finally, we’ll report on the data and build dashboards of our temperature readings using Power BI.
KiZAN will bring 25 Raspberry Pi starter kits that run Windows 10 IoT Core. This will enable participants to build a really compelling IoT/Azure/Power BI story in a single day! Interet of Things (IoT) Raspberry Pi starter kit
We’ll start off the day with an introduction to IoT and build IoT devices (hands on). Next, we’ll build a simple temperature sensor, collecting ambient temperature readings, and stream the data to an Azure IoT Hub.
Once the data is in Azure, we’ll analyze it with Azure Stream Analytics, and ship it to an Azure SQL Database.
Finally, we’ll report on the data and build dashboards of our temperature readings using Power BI.
KiZAN will bring 25 Raspberry Pi starter kits that run Windows 10 IoT Core. This will enable participants to build a really compelling IoT/Azure/Power BI story in a single day! Interet of Things (IoT) Raspberry Pi starter kit
We’ll start off the day with an introduction to IoT and build IoT devices (hands on). Next, we’ll build a simple temperature sensor, collecting ambient temperature readings, and stream the data to an Azure IoT Hub.
Once the data is in Azure, we’ll analyze it with Azure Stream Analytics, and ship it to an Azure SQL Database.
Finally, we’ll report on the data and build dashboards of our temperature readings using Power BI.
Cyberinfrastructure and its Role in ScienceCameron Kiddle
This presentation examines some of the challenges scientists face and describes various cyberinfrastructure technologies that help address these challenges. Example projects employing cyberinfrastructure technologies that we have worked on at the Grid Research Centre, including the GeoChronos project, are also presented. This presentation was given at the IAI International Wireless Sensor Networks Summer School held at the University of Alberta on July 6th, 2009.
These are my slides for the 5 minute overview talk I gave during a recent workshop at the European Commission in Brussels, on the topic of "Big Data Skills in Europe".
Project Natick-Datacenters under sea, a project by microsoft.
Project Natick reflects Microsoft’s ongoing quest for cloud datacenter solutions that offer rapid provisioning, lower costs, high responsiveness, and are more environmentally sustainable.
The tutorial on the Web of Things discusses possible solutions to build the entire vertical system by identifying the relevant components, illustrating their functionality and integration, and showing the examples of existing tools and
systems. First, the tutorial covers architectural aspects and discusses the levels of abstraction for integrating the “things” into the Web. Next, the tutorial focuses on semantic technologies and analytic methods for leveraging services and applications on top of the “things”. State of the art technology and tools are showed through live demos. The tutorial concludes with a brief review of existing projects and an outline of research directions and challenges.
Common Design Elements for Data Movement Eli DartEd Dodds
Eli Dart, Network Engineer ESnet Science Engagement Lawrence Berkeley National Laboratory Cosmology CrossConnects Workshop Berkeley, CA February 11, 2015
Michael Sullivan, M.D. Associate Director, Health Sciences, Internet2
AAMC 2013 Information Technology in Academic Medicine Conference Vancouver CA June 5-7, 2013
This was a 30 min talk intended as one of the opening/overview presentations before a full-day deep dive into ScienceDMZ design patterns and architectures.
Direct downloads are not enabled. Contact me directly (chris@bioteam.net) if you for some odd reason want a copy of this slide deck!
A New Internet Paradigm
Larry Landweber – BBN GPO
Tom Lehman - MAX
Brecht Vermeulen – iMinds, Ghent
Marshall Brinn, Niky Riga - BBN GPO
Rob Ricci - Utah
Active Nets Technology Transfer through High-Performance Network DevicesTal Lavian Ph.D.
Deploying commercial high performance network devices to construct a programmable AN platform
Supporting customizable network intelligences
Supporting excellent AN-specific research projects
Addressing AN and optical networking issues
Shared services - the future of HPC and big data facilities for UK researchMartin Hamilton
Slides from Jisc panel session at HPC & Big Data 2016 with contributions from the Francis Crick Institute, QMUL and King's College London covering their use of the Jisc shared data centre and the eMedLab project
Archiving data from Durham to RAL using the File Transfer Service (FTS)Jisc
From Jisc's campus network engineering for data-intensive science workshop on 19 October 2016.
https://www.jisc.ac.uk/events/campus-network-engineering-for-data-intensive-science-workshop-19-oct-2016
Slides from my talk on R&D innovation projects around the Janet network for the HEAnet / Juniper Innovation Day, September 2015. I talk about some recent Janet R&D initiatives such as our Reach scheme for connecting industry to the network, our end to end performance initiative, and our Safe Share project for secure access to sensitive data by researchers - e.g. medical records. There is also a recap of some of our recent activity around equipment sharing, our shared data centre, connectivity and deals with major cloud providers.
Slides from US Ignite Smart Gigabit Community lighting rounds. Part of the US Ignite Wednesday afternoon sessions at the 2017 Smart Cities Connect conference in Austin Texas.
NSF PI Meeting presentation on US Ignite - Nishal MohanUS-Ignite
Presentation by Dr. Nishal Mohan, National Community Leader of US Ignite. Part of the Monday NSF PI meeting sessions of the Smart Cities Connect conference 2017
New Smart Gigabit Community 2017 announcement - Nishal MohanUS-Ignite
Welcoming new US Ignite Smart Gigabit Communities members for 2017. Part of the US Ignite Tuesday morning sessions at the 2017 Smart Cities Connect conference in Austin Texas.
RFP announcement for new US Ignite Smart Gigabit Cities - Nishal MohanUS-Ignite
Presentation by US Ignite National Community Leader, Dr. Nishal Mohan on the RFP for new members of the Smart Gigabit Communities program. Part of the US Ignite Tuesday morning sessions at the 2017 Smart Cities Connect conference in Austin Texas.
Holograms in Your City: Smart Training, Data Visualization and Communication ...US-Ignite
A demonstration on innovative approaches to education and engagement by Professor of Computer Science at Case Western Reserve University, Mark Griswold. Part of the US Ignite Tuesday morning sessions at the 2017 Smart Cities Connect conference in Austin Texas.
Innovation in Gigcity, Chattanooga TN - Ken HayesUS-Ignite
Director of the Enterprise Center presents on the incredible success story that is Chattanooga Tennessee. Part of the US Ignite Wednesday morning sessions at the 2017 Smart Cities Connect conference in Austin Texas.
Compute for Cancer features an application that harnesses unused computing power in Smart Gigabit Communities and applies the computing power towards efforts to help cure cancer. Part of the US Ignite Wednesday morning sessions at the 2017 Smart Cities Connect conference in Austin Texas.
Towards Wireless-Networked Real-Time Augmented Vision - Hongwei ZhangUS-Ignite
Presentation by Hongwei Zhang, professor of Computer Science at Wayne State University. Part of the US Ignite Wednesday morning sessions at the 2017 Smart Cities Connect conference in Austin Texas.
The Future of Smart & Connected Communities: Driving Science and Community Im...US-Ignite
Erwin Gianchandani, Deputy Assistant Director for Computer & Information Science and Engineering (CISE), National Science Foundation discusses the future of Smart Cities. Part of the US Ignite Tuesday morning sessions at the 2017 Smart Cities Connect conference in Austin Texas.
Data-Driven Green Design Case Studies - Dominique DavisonUS-Ignite
Presentation on as part of the demonstration of PlanIT Impact, a smart gigabit application from Kansas City for enable data-driven green design. Part of the US Ignite Wednesday morning sessions at the 2017 Smart Cities Connect conference in Austin Texas.
Innovation in Phoenix: City on the Rise - Dominic PapaUS-Ignite
Presentation on Innovation in the city of Phoenix, AZ by Dominic Papa, Executive Director of the Arizona Institute for Digital Progress. Part of the US Ignite Wednesday morning sessions at the 2017 Smart Cities Connect conference in Austin Texas.
NSF 16-610* is a notification of opportunities to support, foster, and accelerate fundamental research and education that addresses challenges in enabling Smart & Connected Communities (S&CC)
Next Generation Broadband Cities - Lightning TalksUS-Ignite
Lightning Talks fromMegan Smith U.S. Chief Technology Officer
NIST, OSTP, Tech Hire, Maker Movement, CitySDK, Regional Big Data Hubs, Start-up in a Day, Broadband Connectivity Index, ConectED, Community Gigabit Fund
at the Launch of Smart Gigabit Communities event January 26, 2016
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Science DMZ as a Service: Creating Science Super- Facilities with GENI
1. 1 Beyond Today’s Internet • March 25, 2015
Beyond
Today’s
Internet
Experiencing
a
Smart
Future
Science DMZ as a Service:
Creating Science Super-
Facilities with GENI
Inder Monga, Chin Guok, Eric Pouyoul: ESnet
Ilya Baldin, Paul Ruth: RENCI
Simon Patton, Craig E. Tull: Berkeley Lab
2. 2 Beyond Today’s Internet • March 25, 2015
Inder
Monga
Ilya
Baldine
Simon
Pa2on
Eric
Pouyoul
Chin
Guok
Craig
Tull
4. 4 Beyond Today’s Internet • March 25, 2015
Emerging Trend:
Super Facilities, Coupled by Networks
Experimental
facili*es
are
being
transformed
by
new
detectors,
advanced
mathema*cs,
robo*cs,
automa*on,
advanced
networks.
6. 6 Beyond Today’s Internet • March 25, 2015
• Complexity
of
scien*fic
discovery
increasing
• Data
volumes
are
increasing
>
Moore’s
Law
• Fewer
large
facili*es,
but
global
scien*fic
popula*on
Automated
coupling
of
compute,
storage
with
networks
cri*cal
to
increasing
science
produc*vity
7. 7 Beyond Today’s Internet • March 25, 2015 Slide
from
Craig
E
Tull,
LBL
8. 8 Beyond Today’s Internet • March 25, 2015
Synchrotrons shed new light onto Sciences
Very diverse Science Impacts
9. 9 Beyond Today’s Internet • March 25, 2015
Super-Facility Illustrative Data Flow
Data collection
Transfer to NERSC
Analysis and modeling on NERSC supercomputers:
HipGISAXS simulation HipRMC fitting
FFT
Compare
start
with
random
system
move
parCcle
random
Autotuning
On-the-fly
calibration,
processing
Combining:
GIXSGUI, dpdak + …
Real-time
access via web portal
10. 10 Beyond Today’s Internet • March 25, 2015
Global data movement infrastructure
has challenging end-to-end requirements
Physical
pipe
that
leaks
water
at
rate
of
.0046%
by
volume.
è
è
Network
‘pipe’
that
drops
packets
at
rate
of
.0046%.
è
è
Result
100%
of
data
transferred,
slowly,
at
<<5%
opCmal
speed.
Result
99.9954%
of
water
transferred,
at
“line
rate.”
essenCally
fixed
determined
by
speed
of
light
Through
careful
engineering,
we
can
minimize
packet
loss.
AssumpCons:
10Gbps
TCP
flow,
80ms
RTT.
See
Eli
Dart,
Lauren
Rotman,
Brian
Tierney,
Mary
Hester,
and
Jason
Zurawski.
The
Science
DMZ:
A
Network
Design
Pa`ern
for
Data-‐
Intensive
Science.
In
Proceedings
of
the
IEEE/ACM
Annual
SuperCompuBng
Conference
(SC13),
Denver
CO,
2013.
12. 12 Beyond Today’s Internet • March 25, 2015
Representative Science DMZ
13. 13 Beyond Today’s Internet • March 25, 2015
• Current implementations deploy dedicated DTNs in the Science DMZ
– Manual configuration and tuning
– Sharing is scheduled, many times manually
Dedicated vs. Virtual resources
14. 14 Beyond Today’s Internet • March 25, 2015
Dedicated
vs.
Virtual
resources
• GENI
provides
a
distributed
solware-‐defined
infrastructure
(SDI)
– Compute
+
Storage
+
Network
15. 15 Beyond Today’s Internet • March 25, 2015
• GENI provides a distributed software-defined infrastructure (SDI)
– Compute + Storage + Network
• GENI racks may be deployed on-campus or in provider networks close
to the campus
• ‘Science DMZ as a service’
– Applications can provision a virtual ‘Science DMZ’ as and when needed
Dedicated vs. Virtual resources
Programmable
infrastructure
to
enable
end-‐users
to
create
dynamic
‘fric*on-‐free’
infrastructures
without
advanced
knowledge/training
16. 16 Beyond Today’s Internet • March 25, 2015
Today’s Demonstration:
Real-time data processing and vis. workflow
h`p://portal.nersc.gov/project/als/sc14/
Data
from
ALS
Experiment
SPADE
instance
@
Server
at
Argonne
ExoGENI
SPADE
VM
@
Starlight,
Chicago
ESnet
ExoGENI
SPADE
VM
@
Oakland,
California
Compute
Cluster
NERSC,
LBL
AL2S,
ESnet
• WAN-‐op*mized
data
transfer
nodes
and
a
network
slice
created
programma*cally
(Science
DMZ
as
a
service)
• Applica*on
workflow
instan*ated
to
stage
data
at
the
GENI
rack
on
Science
DMZ
slice
• Data
is
moved
op*mally
across
the
WAN1
1
Earlier
work,
like
Phoebus,
have
instanCated
the
value
of
this
approach
17. 17 Beyond Today’s Internet • March 25, 2015
Microtomography of High Temperature
Materials under stress
Set
collected
by
materials
scienCst
Rob
Ritchie,
LBNL/UCB
18. 18 Beyond Today’s Internet • March 25, 2015
Summary and Future
• Many
science
applicaCons
are
now
building
coupled
workflows
across
the
WAN
to
Ce
together
specialized
science
instruments
for
‘big
data’
discovery
• Programmability
extends
to
the
enCre
‘Solware-‐defined
infrastructure’
stack
as
illustrated
by
GENI
racks
• WAN
‘Infrastructure
as
a
Service’
soluCons
will
become
pervasive,
as
programmability
and
virtualizaCon
moves
outside
the
data
center
domain.
• We
think
of
the
‘network’
as
an
‘instrument’
that
enables
scienCfic
discovery
• Should
there
be
a
disCncCon
between
a
network
and
a
data
center?
• What
will
the
next
generaCon
WAN
look
like?
19. 19 Beyond Today’s Internet • March 25, 2015
THANK
YOU
Acknowledgements:
DOE
ASCR
Funding
and
Support,
Jason
Lee,
Brent
Draney
and
NERSC
networking
team,
Dula
Parkinson
(ALS),
Linda
Winkler
and
Argonne
Networking
Team
(ANL)
imonga@es.net,
ibaldin@renci.org,
cetull@lbl.gov