The document discusses the challenges of achieving accurate gene expression results using rt-pcr and introduces a new gene expression workflow developed by Qiagen. This workflow includes the Quantinova® reverse transcription kit and the Rotor-Gene® Q for optimized cDNA synthesis and reliable PCR quantification. It also highlights the integration of results into Qiagen's Geneglobe center for further analysis and insights into biological interactions and disease processes.

1. Accurate and biologically relevant results in RT-PCR-based
gene expression can be difficult to achieve. Successful
transcriptome work requires validated, reproducible targets
and high-quality technology.
Recognizing the variability arising from sample physiology
and pathology, the influence of sample purification and
assay conditions, and the importance of access to easy-
to-use software, QIAGEN experts developed a new gene
expression workflow. It will help you properly validate your
RT-PCR and gain the deepest insight into your result.
Discover more at www.qiagen.com/gene-expression.
Deeper Insight into
Transcriptomes!
Discover a new workflow for RT-PCR-based gene expression work
Sample to Insight

2. Start with fast and efficient reverse transcription
The new QuantiNova®
Reverse Transcription Kit yields cDNA that is optimal
for real-time PCR, allowing reliable quantification of targets from all regions
of mRNA transcripts. It enables you to
• Eliminate artifacts thanks to the gDNA Removal Mix
• Monitor cDNA synthesis using an internal control
• Synthesize wide linear range of cDNA
From real-time PCR to multiple results
The Rotor-Gene®
Q provides outstanding performance and reliable results in
real-time PCR. CT data generated can be uploaded directly to our GeneGlobe
Center where you can calculate gene and miRNA expression results and copy
number measurements and identify somatic mutations.
Finish with ingenious analyses
To investigate the possible biological interactions of differently regulated
genes, import your GeneGlobe results into our Ingenuity Pathway Analysis
software, for reliable further analyses. We’ll help you reach a deeper
understanding of metabolomics, proteomics and RNA sequence data,
identify upstream regulators, and make discoveries about disease processes.
For up-to-date licensing information and product-specific disclaimers, see the respective QIAGEN kit handbook or user manual. QIAGEN kit handbooks and user
manuals are available at www.qiagen.com or can be requested from QIAGEN Technical Services or your local distributor.
Trademarks: QIAGEN®
, Sample to Insight®
, QuantiNova®
, Rotor-Gene®
(QIAGEN Group)
1099960 12/2015 © QIAGEN, all rights reserved.
www.qiagen.com
Sample to Insight