This document is a curriculum vitae for Emmanuel Dupont. It provides biographical information such as his date of birth, education history, employment history, research interests, teaching experience, and publications. His research has focused on gap junctions and their role in cell-to-cell communication, particularly in relation to the cardiovascular system.

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CURRICULUM VITAE
NAME: DUPONT, Emmanuel
DATE OF BIRTH: January 18th, 1960
PRIVATE ADDRESS: 1 Hill View court, Woking, Surrey, GU22 7QN,
UK.
NATIONALITY: French
PROFESSIONAL ADDRESS: Myocardial Function, National Heart and Lung
Institute, Imperial College,
Imperial Centre for Translational and
Experimental Medicine, 4th floor,
Hammersmith Campus, Du Cane Road,
London W12 0NN
e.dupont@imperial.ac.uk
http://www.imperial.ac.uk/people/e.dupont
https://uk.linkedin.com/in/dupontemmanuel
Mob: 07522373612
ACADEMIC RECORD
 University of Poitiers, U.F.R. Sciences (Oct 1979 to Sept 1986)
• Bachelors degree (license) in Cell Biology and Physiology (with distinctions)
1983
• Masters degree (maitrise) in Physiology and Pharmacology, 1984
• Post-Graduate Diploma (DEA) (with distinction) 1985
 University of Aix-Marseille II, Sciences Faculty, Laboratoire de Biologie de la
Differenciation Cellulaire, LA CNRS 179. Luminy (Oct 1986 to July 1989).
• PhD : Gap Junctions (or communicating junctions): Use of Antipeptide Antibodies
for the Immunodetection and Immunopurification of Junctional Proteins.
 Society Memberships: British Society for Cell Biology.
RESEARCH CAREER AND CURRENT POST
 Research Associate (with Prof. J.E. Trosko): Department of Pediatrics/Human
Development, Michigan State University, East Lansing, USA (Oct.1989-Sept.1992).
 Research and Teaching Assistant (with Prof. P. Meda): Centre Medical Universitaire,
Geneva, Switzerland (Oct. 1992-Oct.1994).
 Research Fellow: From February 1995, working with Prof. N. J. Severs, Cardiac
Medicine, National Heart and Lung Institute, Imperial College London, London, UK.
 Promoted to RAII grade, October 1998, Cardiac Medicine, National Heart and Lung
Institute, Imperial College London.
 Promoted to Research Lecturer, March 2000, Cardiac Medicine, National Heart and
Lung Institute, Imperial College London. Supported as a Principal Investigator by British
Heart Foundation project grants.
 Senior Lecturer in Cardiovascular Cell Biology, Biochemistryand Physiology
department, Faculty of Health and Medical Sciences, University of Surrey, Guildford,
UK. (Oct 2009-Oct 2012).

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 Honorary Research Fellow, National Heart and Lung Institute, Imperial College London,
London, UK, (Oct 2009-Oct 2012).
 Senior Research Fellow: working with Prof. N.S. Peters, Myocardial Function, National
Heart and Lung Institute, Imperial College London, London, UK. Since 1st of November
2012.
 Member of the BHF ICTEM Studentship Consortium, Sept 2014
 Member of the BHF Centre of Research Excellence, Sept 2014
RESEARCH ACTIVITY
My research interest centres on gap junctions, the structures responsible for direct cell-
to-cell communication and coupling. I started work in this area with studies on cardiac
gap junctions for my post-graduate diploma and my PhD. We generated antibodies
against the main gap junction protein expressed in heart in order to perform structural
studies of the molecular organisation of cardiac gap junctions. This work included
purification of the antibodies, and their application to studies on the topology and
intramolecular interaction of connexins. After my PhD, I moved to Michigan State
University to study the relationship between gap junctional communication and control of
growth and differentiation using techniques in cell culture and molecular biology. Having
acquired expertise in these areas, I sought to build on my knowledge in aspects of
physiology relevant to gap junctions, and to this end secured a position as a Research
and Teaching Assistant at Geneva Medical School. There I worked for a two-year period
on the relationship between gap junctional communication and insulin secretion in
pancreatic  cells using antisense oligonucleotides. From here, I joined Prof. N. Severs'
group on short-term funding to initiate studies on connexin expression in the heart and
cardiovascular disease using biochemical and molecular techniques in combination with
modern morphological analysis.
Over the last 6-7 years, I have developed a number of transfected cell models to study
particularly the co-expression of multiple connexins. The recent addition of HL-1 clones
in the panel of cell models in conjunction with RNA interference allow to study directly
the influence of gap junction composition on action potential propagation in two
dimensional cultures.
These systems permit or will permit to study experimentally a large number of
parameters that are thought to play a role in arrhythmia (e.g. the geometry of the culture,
the co-expression of connexins, the interactions of Ca2+ handling with the passive,
junctional resistance etc…) on a completely controlled cell system. These cell systems
are also amenable to study other aspect of gap junction biology such as trafficking,
oligomerisation, molecular permeability, connexin compatibility, associated molecules
etc…. Because more than one cell type is now available, specific regulation that
depends on the tissue rather than the connexin can be examined by comparing the
same connexin(s) in the different transfectants (epithelial cells, myocytes)
This has already led to six PhD theses (Drs D Halliday, N Thomas, N Charolidi, P Dias,
K Griksheit and R Chowdhury).
I have over 26 years’ experience in the gap junction field and, among them, over 20
years in relation to the cardiovascular system. I have also mastered and have
experience in a large range of cell biology techniques including antibody generation and
characterisation using biochemical techniques such as Western blotting, affinity
purification, ELISA and immunohistochemistry; structural biochemistry using antibody
probes, electrophoresis (SDS-PAGE, non-denaturing gels, gradient gels), peptide
mapping; classical chromatography techniques such as gel filtration, ion exchange,
reverse phase using low pressure, HPLC or FPLC; cell culture techniques (primary cells

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and cell lines, transfection, siRNA inhibition of gene expression); molecular biology
techniques such as PCR, subcloning into plasmids, generation of mammalian
expression plasmids, bacteria transformation, Northern and Southern blotting,
generation of DNA and RNA probes with or without radioactivity, in situ hybridisation.
TEACHING EXPERIENCE
Geneva Medical School
Routine undergraduate teaching duties (supervision of practical classes of histology for
the first and second year medical students. Includes examination of the histology of all
major systems: brain, lung, kidney, male and female reproduction, heart and blood
vessels, various endo- and exocrine glands, etc).
Imperial College London
Formal lecturing since 2001: Cell-Cell transmission/conduction/Gap junction
electrophysiology for the Cardiopulmonary BSc Foundation course, for the
Cardiovascular Science BSc and for the MRes in Biomolecular Research.
BSc student supervision: Supervision of ~ 10 weeks research projects, 10 students
since 2001.
Marking of exam papers and BSc reports.
University of Surrey
Co-ordinator of the dissertation module of the Clinical Biochemistry MSc.
Co-ordinator of the dissertation module of the Pharmaceutical Medicine/Clinical
Pharmacology MSc.
Co-ordinator of the research module of the Pharmaceutical Medicine/Clinical
Pharmacology MSc.
Member of 2 boards of examination (Clinical Biochemistry and Pharmaceutical
Medicine/Clinical Pharmacology MSc).
Member of 2 boards of study (Clinical Biochemistry and Pharmaceutical
Medicine/Clinical Pharmacology MSc).
Main supervisor of BSc and MSc students for their dissertation projects (7 students).
Marking of literature reviews and dissertations of BSc and MSc students.
PhD Thesis examination
Dr S Alcolea, La connexine 45 dans le myocarde de souris. Patron d’expression
et approche de son role par substitution genetique. Marseille, France, November 28th
2002. Examiner
Dr F Duthe, Influence de l’ATP et des ions Mg sur la communication jonctionnelle
intercellulaire de myocytes ventriculaires de rat nouveau ne: implication de proteines
phosphatases. Poitiers, France, December 16th 2002. Examiner and report writer.
Dr I Plaisance, Importance du cytosquelette et des processus de
phosphorylation/dephosphorylation des proteines dans la regulation fonctionnelle des
canaux intercellulaires formes de connexine 43. Poitiers, France, December 19th 2003.
Examiner and report writer.
Dr C Louault, Role des fibroblastes dans le remodelage cellulaire cardiaque.
Poitiers, France, June 13th 2007. Examiner and report writer.

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Higher degree supervision
During my periods at Michigan State University, at Geneva Medical School and at the
NHLI, I have assisted the respective laboratory heads with the day-to-day technical
training and supervision of research students under their formal supervision. This input
was important for example in the supervision of the Masters degree of Miss S. C. Lu in
Michigan State, of the PhD thesis of Dr C. Vozzi in Geneva. I was very strongly involved
in the supervision of Dr D Halliday’s PhD (under the formal supervision of Prof NJ
Severs, 2003).
I have officially co-supervised three PhD students with Prof NJ Severs (IC), Dr N
Thomas (August 2007), Dr N Charolidi (Oct 2008) and Dr K Grikscheit (March 2010),
one with Dr K McLeod (IC), Dr P Dias (January 2011), two with Prof N Peters (IC), Dr R
Chowdhury (July 2011) and Dr F-S Ng (September 2011) and two with Dr E Oviedo-Orta
(UoS), Dr RJ Anicattu Issac (December 2011) and Dr R Mohamed (July 2012).
OTHER PROFESSIONAL ACTIVITIES
Reviewer for the FASEB J, Interface Focus, J Mol Cell Cardiol, J Cardiovas Trans Res,
BBA, Mol Cell Biochem, Europace, J Gastroenterology and Hepatology, Int J Exp
Pathol.
Reviewer for several UK and international grant funding bodies (including the BHF).

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RESEARCH GRANTS
Because of my position (short-term contracts), I was not eligible to apply in my name for
many grant schemes. However, I have been involved in the writing of many successful
applications since I joined Imperial College. These are detailed below:
1- Expression of gap junction connexins in heart disease: preliminary study. Clinical
Research Committee of the Royal Brompton NHS Trust/NHLI, eight months’ salary
support to E Dupont, Feb-Sept 1995, £ 23,107.
Co-written with NJ Severs, held by NJ Severs.
2- Expression of gap junction connexins in heart disease: development of connexins
mRNA probes. Fellowship of the European Society of Cardiology, six months’ daily
subsistence to E Dupont, Sept 1995-March 1996, paid in two instalments FF 50,000
each.
Co-written with NJ Severs, held by E Dupont.
3- Expression of gap-junctional connexins in human heart disease. The Wellcome Trust,
project grant, March 1996-March 1999, £ 147,000 (includes salary for E Dupont).
Co-written with NJ Severs, held by NJ Severs and E Dupont.
4- Relating connexin expression and connexon structure to conduction properties in the
heart: an in vitro transfected cell model. The British Heart Foundation, project grant,
March 1999-March 2002, £ 171,000 (includes salary for E Dupont).
Co-written with SR Coppen and NJ Severs, held by NJ Severs.
5- Excitation-contraction coupling mechanism in hibernating myocardium. Clinical
Research Committee of the Royal Brompton NHS Trust/NHLI, six months’ salary
support to D Gathercole, June 1999-Nov1999, £ 18,500.
Co-written with D Gathercole and NJ Severs.
6- Function of cardiac gap junction. The Medical Research Council, Research
studentship for D Halliday, Oct 1999-Oct 2002, £ 25,141.
Co-written with NJ Severs, held by NJ Severs.
7- Connexin 45 and 40 in the normal and diseased human heart. The British Heart
Foundation, PhD studentship (clinical) for R Kaba, Nov1999-Nov2002, £ 166,245.
Co-written with R Kaba, SR Coppen and NJ Severs, held by NJ Severs.
8- Intercellular signalling through connexin channels: a key to the understanding of
cardiovascular disease. European Union, RTD activities of a generic nature: quality of
life and management of living resources, Jan 2000-Jan2003, £ 213,847 (include salary
for A Rama).
Co-written with SR Coppen and NJ Severs, held by NJ Severs.
9- Gap junction, connexin expression and susceptibility to post-operative atrial
fibrillation. The British Heart Foundation, project grant, for two years, £ 98,826.
Co-written with SR Coppen and NJ Severs, held by NJ Severs.

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10- Transfected cell models to investigate connexin co-expression in the cardiovascular
system. The British Heart Foundation, project grant, Dec 2003-Dec 2005, £196,000.
Co-written with NJ Severs, held by NJ Severs.
11- Transfected cell models to investigate connexin co-expression in the heart. The
NHLI Foundation, PhD studentship, Oct 2003-Oct 2006, £ 26,000 and the student
bursary for Dr N Thomas.
Co-written with NJ Severs, held by NJ Severs and E Dupont.
12- Gap-junctional communication and the control of vascular muscle cell phenotype.
The NHLI Foundation, PhD studentship, Oct 2004-Oct 2007, student bursary for Dr N
Charolidi.
Co-written with NJ Severs, held by NJ Severs and E Dupont.
13- Mechanism of action potential generation and propagation investigated in a
genetically engineered cell model. The British Heart Foundation, project grant, Dec
2005-Dec 2008, £265,962.
Co-written with NJ Severs and K McLeod, held by E Dupont.
14- Genetically engineered cell models to investigate the mechanisms of pacemaker
activity and electrical propagation. The NHLI Foundation, PhD studentship, Oct 2006-
Oct 2009, student bursary for Miss P Dias.
Co-written with K McLeod, held by K McLeod and E Dupont.
15- An In silico model of action potential propagation, biologically validated in the HL1-6
myocyte cell line: A framework for characterising myocardial re-entry and fibrillation in
the human heart. The British Heart Foundation, project grant, £296,235, Result
expected mid-October.
Co-written with C Cantwell and NS Peters, Principal Investigator E Dupont
PUBLICATIONS
Peer review articles
1-Immunological Characterization of Rat Cardiac Gap Junctions. Presence of Common
Antigenic Determinants in Heart of Other Vertebrates Species and in Various Organs.
E. Dupont, A.H. El Aoumari, S. Roustiau-Severe, J.P. Briand and D. Gros.
J.Membr.Biol., 104, 119-128,(1988).
2-Cross-linking of Cardiac Gap Junction Connexons by Thiol/Disulfide Exchanges.
E. Dupont, A.H. El Aoumari, J.P. Briand, C. Fromaget and D. Gros. J.Membr.Biol., 108,
247-252,(1989).
3-Conservation of a Cytoplasmic Carboxy-Terminal Domain of Connexin 43, a Gap
Junctional Protein, in Mammal Heart and Brain.
A.H. El Aoumari, C. Fromaget, E. Dupont, H. Reggio, P. Durbec, J.P. Briand, K. Boller,
B. Kreitman and D.Gros. J.Membr.Biol., 115, 229-240,(1990).
4-Changes in the Expression of Connexin 43, a Cardiac Gap Junctional Protein, During
Mouse Heart Development.

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C. Fromaget, A.H. El Aoumari, E. Dupont, J.P. Briand and D. Gros. J.Mol.Cell.Cardiol.,
22, 1245-1258,(1990).
5-Affinity Purification of a Rat Brain Junctional Protein: Connexin 43.
E. Dupont, A.H. El Aoumari, C. Fromaget, J.P. Briand and D. Gros. Eur.J.Biochem., 200,
263-270,(1991).
6-Immunolocalization of an Extracellular Domain of Cx 43 in Rat Heart Gap Junctions.
A.H. El Aoumari, E. Dupont, C. Fromaget, T. Jarry, J.P. Briand, B. Kreitman and D.
Gros. Eur.J.Cell Biol., 56, 391-400,(1991).
7-Characterization of Gap Junctional Communication-Deficient Mutants of a Rat Liver
Epithelial Cell Line.
S.Y. Oh, E. Dupont, B.V. Madhukar, J.P. Briand, C.C. Chang, E. Beyer and J.E. Trosko.
Eur.J.Cell Biol., 60, 250-255,(1993).
8-Gap Junctional Intercellular Communication in Mouse Lung Epithelial Cell Lines:
Effects of Cell Transformation and Tumor Promoters.
R. Chaudhuri, K. Sigler, E.Dupont, J.E.Trosko, A.M. Malkinson and R.J. Ruch. Cancer
Letters, 71, 11-18,(1993).
9-Immortalized Hypothalamic Luteinizing Hormone-Releasing Hormone Neurons
Express a Connexin 26-like Protein and Display Functional Gap Junction Coupling
Assayed by Fluorescence Recovery after Photobleaching.
D.F. Matesic, J.A. Germak, E. Dupont and B.V. Madhukar. Neuroendocrinology, 58,
485-492,(1993).
10-Restoration of Gap Junctional Intercellular Communication in a Communication
Deficient Rat Liver Cell Mutant by Transfection with Connexin 43 cDNA.
Y.S. Jou, E. Dupont, S.C. Lu, B.V. Madhukar, S.Y. Oh, J.E. Trosko and C.C. Chang.
Mol.Carcinogenesis, 8, 234-244,(1993).
11-Loss of Gap Junctions from DDT-Treated Rat Liver Epithelial Cells.
R.J. Ruch, W.J. Bonney, K. Sigler, X. Guan, D.F. Matesic, L.D. Scafer, E. Dupont and
J.E. Trosko. Carcinogenesis, 15, 301-306,(1994).
12-Topography of Mammalian Connexins in Human Skin.
D. Salomon, E. Masgrau, S. Vischer, S. Ullrich, E. Dupont, T. Sappino, J.H. Saurat and
P. Meda. J.Invest.Dermatol, 103, 240-247,(1994).
13-Gap Junction Localization and Connexin Expression in Cytochemically Identified
Endothelium Cells from Arterial Tissue.
H.-I Yeh, E. Dupont, S. Coppen, S. Rothery and N.J. Severs.
J. Histochem. Cytochem., 45, 539-550, (1997).
14-Hyperinsulinemia-Induced Hypoglycemia Is Enhanced by Overexpression of
Connexin 43.
C. Vozzi, D. Bosco, E. Dupont, A. Charollais and P. Meda. Endocrinology, 138, 2879-
2885, (1997).

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15-Angiotensin II Receptor Type I mRNA is Upregulated in Atria of Patients with End-
Stage Heart Failure.
R.R. Kaprielian, E. Dupont, S. Hafizi, P.A. Poole-Wilson, A. Khaghani, M. H. Yacoub
and N.J. Severs. J.Mol.Cell.Cardiol., 29, 2299-2304, (1997).
16-Upregulation of Connexin43 Gap Junctions Between Smooth Muscle Cells after
Balloon Catheter Injury in the Rat Carotid Artery.
H.I. Yeh, F. Lupu, E. Dupont, N.J. Severs. Arterioscler.Thromb.Vasc.Biol., 17, 3174-
3184, (1997).
17-Down-Regulation of Immunodetectable Connexin43 and Decreased Gap Junction
Size in the Pathogenesis of Chronic Hibernation in the Human Left Ventricle.
R.R. Kaprielian, M. Gunning, E. Dupont, M.N. Sheppard, S.M. Rothery, R. Underwood,
D.J. Pennel, K. Fox, J. Pepper, P.A. Poole-Wilson and N.J. Severs. Circulation, 97, 651-
660, (1998).
18-Identification of Two Further Gap-Junctional Proteins, Connexin 40 and Connexin 45,
in Human Myometrial Smooth Muscle Cells at Term.
W.M. Kilarski, E. Dupont, S. Coppen, H.-I Yeh, R.G. Gourdie, M. Rezapour, U. Ulmsten,
G.M. Roomans and N.J. Severs. Eur.J.Cell Biol., 75, 1-8, (1998).
19-Connexin45 is Preferentially Associated with the Ventricular Conduction System in
Mouse and Rat Heart.
S.R. Coppen, E. Dupont, S. Rothery and N.J. Severs. Circ. Res., 82, 232-243, (1998).
20-Individual Gap Junction Plaques Contain Multiple Connexins in Arterial Endothelium.
H.-I Yeh, S. Rothery, E. Dupont, S. R. Coppen and N. J. Severs. Circ. Res., 83, 1248-
1263, (1998).
21-Connexin Make-up of Endothelial Gap Junction in the Rat Pulmonary Artery as
Revealed by Immunoconfocal Microscopy and Triple-label Immunogold Electron
Microscopy.
Y.-S. Ko, H.-I Yeh, S. Rothery, E. Dupont, S. R. Coppen and N. J. Severs. J. Histochem.
Cytochem., 47, 638-691, (1999).
22-Chamber-related Differences in Connexin Expression in the Human Heart.
E. Dupont, C. Vozzi, S. R. Coppen, H.-I Yeh and N. J. Severs. J.Mol.Cell.Cardiol., 31,
991-1003, (1999).
23-Differential Expression of Connexin43 and Desmin Defines Two Subpopulations of
Medial Smooth Muscle Cells in the Human Mammary Artery.
Y.-S. Ko, H.-I Yeh, M. Haw, E. Dupont, R. Kaba, G. Plenz, H. Robenek and N. J.
Severs. Arterioscler.Thromb.Vasc.Biol., 19, 1669-1680, (1999).
24- Dephosphorylation Agents Depress Gap Junctional Communication Between Rat
Cardiac Cells Without Modifying the Connexin43 Phosphorylation Degree.
F. Duthe, E. Dupont, F.Verrecchia, I. Plaisance, N.J.Severs, D. Sarrouilhe and
J.C.Hervé. Gen. Physiol. Biophys., 19, 441-449, (2000).
25- Regional differentiation of desmin, connexin43 and connexin45 expression patterns
in rat aortic smooth muscle.

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Y.-S. Ko, S.R. Coppen, E. Dupont, S. Rothery, N.J. Severs. Arterioscler. Thromb. Vasc.
Biol., 21, 355-364, (2001).
26- Altered Connexin Expression in Human Congestive Heart Failure.
E. Dupont, T. Matsushita, R.A. Kaba, C. Vozzi, S.R. Coppen, N. Khan, R. Kaprielian,
M.H. Yacoub and N. J. Severs, J. Mol. Cell. Cardiol., 33, 359-371, (2001).
27- The Gap-junctional Protein Connexin40 Is Elevated in Patients Susceptible to
Postoperative Atrial Fibrillation.
E. Dupont, Y.-S. Ko, S. Rothery, S.R. Coppen, M. Baghai, M. Haw and N.J. Severs.
Circulation, 103, 842-849, (2001).
28- Comparison of Connexin 43, 40 and 45 Expression Patterns in the Developing
Human and Mouse Heart.
R.A. Kaba, S.R. Coppen, E. Dupont, J.N. Skepper, S. Elneil, M.P. Haw, J.R. Pepper,
M.H. Yacoub, S. Rothery and N.J. Severs. Cell Commun. Adhes., 8, 339-43, (2001).
29- Multiple Connexins Localized to Individual Gap-Junctional Plaques in Human
Myometrial Smooth Muscle.
W.M. Kilarski, S. Rothery, G.M. Roomans, U. Ulmsten, M. Rezapour, S. Stevenson, S.R.
Coppen, E. Dupont, N.J. Severs. Microsc Res Tech, 54,114-22, (2001).
30- Gap Junctions and Connexin Expression in Human Suburothelial Interstitial Cells.
G.P. Sui, S. Rothery, E. Dupont, C.H. Fry, N.J. Severs. BJU Int., 90,118-29, (2002).
31- Alteration of Gap Junctions and Connexins in the Right Atrial Appendage During
Cardiopulmonary Bypass.
H.I Yeh, S.H. Hou, H.R. Hu, Y.N. Lee, J.Y. Li, E. Dupont, S.R. Coppen, Y.S. Ko,
N.J.Severs, C.H. Tsai. J Thorac Cardiovasc Surg., 124, 1106-12, (2002).
32- Comparison of Connexin Expression Patterns in the Developing Mouse Heart and
Human Foetal Heart.
S.R. Coppen, R.A. Kaba, D. Halliday, E. Dupont, J.N. Skepper, S. Elneil, N.J. Severs.
Mol Cell Biochem., 242, 121-7, (2003).
33- Impedance Measurements and Connexin Expression in Human Detrusor Muscle
from Stable and Unstable Bladders.
G.P. Sui, S.R. Coppen, E. Dupont, S. Rothery, J. Gillespie, D. Newgreen, N.J. Severs,
C.H. Fry. BJU Int., 92, 297-305, (2003).
34- Development of a Cell Model for Functional and Structural Analysis of Connexin Co-
Expression: Achieving Homogeneous and Inducible Expression of Multiple Connexins in
Stable Transfectants.
D. Halliday, E. Dupont, S.R. Coppen, N.J. Severs. Cell Commun Adhes., 10, 311-7,
(2003).
35- Cardiac Connexins Cx43 and Cx45: Formation of Diverse Gap Junction Channels
with Diverse Electrical Properties.
T. Desplantez, D. Halliday, E. Dupont, R. Weingart. Pflugers Arch., 448, 363-75, (2004).
36- Sex differences in connexin-43 expression in left ventricles of aging rats.N.

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Tribulová, E. Dupont, T. Soukup, L. Okruhlicová, N.J. Severs. Physiol Res., 54, 705-8,
(2005).
37- Human atrial conduction and arrhythmogenesis correlates with conformational
exposure of specific epitopes on the connexin40 carboxyl tail.
P. Kanagaratnam, E. Dupont, S. Rothery, S.R. Coppen, N.J. Severs, N.S. Peters. J Mol
Cell Cardiol., 40, 675-87, (2006).
38- Up-regulation of connexin43 correlates with increased synthetic activity and
enhanced contractile differentiation in TGF-β-treated human aortic smooth muscle cells.
A. Rama, T. Matsushita, N. Charolidi, E. Dupont, N.J. Severs. Eur J Cell Biol., 85, 375-
86, (2006).
39- Loss of desmoplakin isoform I causes early onset cardiomyopathy and heart failure
in a Naxos-like syndrome.
A. Uzumcu, E.E. Norgett, A. Dindar, O. Uyguner, K. Nisli, H. Kayserili, S.E. Sahin, E.
Dupont, N.J. Severs, I.M. Leigh, M. Yuksel-Apak, D.P. Kelsell, B. Wollnik. J Med Genet.,
43, e5, (2006).
40- Relationship of connexin43 expression to phenotypic modulation in cultured human
aortic smooth muscle cells.
T. Matsushita, A. Rama, N. Charolidi, E. Dupont, N.J. Severs. Eur J Cell Biol., 86, 617-
28, (2007).
41- Gap junction remodelling in human heart failure is associated with increased
interaction of connexin43 with ZO-1.
A.F. Bruce, S. Rothery, E. Dupont, N.J. Severs. Cardiovasc Res., 77, 757-65, (2008).
42- Coexpression of connexin 45 with connexin 43 decreases gap junction size.
K. Grikscheit, N. Thomas, A.F. Bruce, S. Rothery, J. Chan, N.J. Severs, E. Dupont. Cell
Commun Adhes., 15, 185-93, (2008).
43- Human connexin31.9, unlike its orthologous protein connexin30.2 in the mouse, is
not detectable in the human cardiac conduction system.
M.M. Kreuzberg, M. Liebermann, S. Segschneider, R. Dobrowolski, H. Dobrzynski,
R. Kaba, G. Rowlinson, E. Dupont, N.J. Severs, K. Willecke. J Mol Cell Cardiol., 46,
553-559, (2009).
44- Influence of a 6-his-tag on the properties of cardiac gap junction channels.
T. Desplantez, D. Halliday, E. Dupont, N.J. Severs, R. Weingart. J Membr Biol, 240,
139-50, (2011).
45- Cytoplasm resistivity of mammalian atrial myocardium determined by
dielectrophoresis and impedance methods. Fry,C.H., Salvage,S.C., Manazza,A.,
Dupont,E., Labeed,F.H., Hughes,M.P., and Jabr,R.I.. Biophys. J. 103, 2287-2294,
(2012).
46- Characterisation of Connexin Expression and Electrophysiological Properties in
Stable Clones of the HL-1 Myocyte Cell Line. Dias,P., Desplantez,T., El-Harasis,M.A.,

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Chowdhury,R.A., Ullrich,N.D., Cabestrero de,D.A., Peters,N.S., Severs,N.J.,
MacLeod,K.T., and Dupont,E. PLoS one 9, e90266, (2014).
47- Architectural correlates of myocardial conduction: changes to the topography of
cellular coupling, intracellular conductance, and action potential propagation with
hypertrophy in Guinea-pig ventricular myocardium. Fry,C.H., Gray,R.P., Dhillon,P.S.,
Jabr,R.I., Dupont,E., Patel,P.M., and Peters,N.S. Circ. Arrhythm. Electrophysiol. 7,
1198-1204, (2014).
48- Relating Specific Connexin Co-Expression Ratio to Connexon Composition and Gap
Junction Function. Desplantez,T., Grikscheit,K., Peters,N.S., Severs,N.J., and Dupont,E.
(2015). In revision.
49- Biochemical and electrical characterisation of tandem cardiac connexin.
T. Desplantez, N. Thomas, N.J. Severs, R. Weingart, E. Dupont. submitted.
50- Gap junction-mediated intercellular communication in co-expressing cells is
regulated by isotype-specific transcriptional and post-translational mechanisms.
N.M. Thomas, R. Grey, C.H. Fry, K.T. Macleod, S. Rothery, N. J. Severs, E. Dupont.
submitted.
51- siRNA knock-down of Cx43 in cultured human aortic smooth muscle cell leads to a
large reduction of migratory activity.
N. Charolidi, N.J. Severs, E. Dupont. In preparation.
52- Suppression of Cx45 using siRNA in HL-1 cardiomyocytes leads to a larger
reduction in action potential propagation velocity than suppression of Cx40 and/or Cx43.
P. Dias, T. Desplantez, K.T. Macleod, N.J. Severs, E. Dupont. In preparation.
Book chapters, reviews
1-Chemical Modulation of Gap Junctional Intercellular Communication in Vitro: An in
Vitro Biomarker of Epigenetic Toxicology.
J.E. Trosko, C.C. Chang, E. Dupont, B.V. Madhukar and G. Kalimi. In Vitro methods in
toxicology, 465-478,(1992).
2-Suppression of Gap Junctional Gene Expression by Growth Factors and TPA in
Human Epidermal Keratinocytes in Vitro.
E. Dupont, B.V. Madhukar and J.E. Trosko. Progress in Cell Research, Vol 3, 321-
327,(1993).
3-Oncogenes, Tumor Suppressor Genes, and Intercellular Communication in the
Oncogeny as Partially Blocked Ontogeny Hypothesis.
J.E. Trosko, C.C. Chang, B.V. Madhukar and E. Dupont. in : "New Frontiers in Cancer
Causation" IVERSEN editor, TAYLOR and FRANCIS publishers, Washington DC, 181-
197,(1993).

12. 12
4-Gland Cell Connexins.
P. Meda, C. Vozzi, S. Ullrich, E. Dupont, A. Charollais, E. Sutter, D. Bosco. Progress in
Cell Research, Vol. 4, 281-287, (1995).
5-Intercellular Communication: A Paradigm for the Interpretation of the
Initiation/Promotion/Progression Model of Carcinogenesis.
J. E. Trosko, C. C. Chang, B. V. Madhukar and E. Dupont. In: "Chemical induction of
cancer", Edited by J. C. Arcos, Birkhauser publisher, 205-225, (1996).
6- Gap Junction and Connexins in the Cardiovascular System.
N. J. Severs, E. Dupont, R. R. Kaprielian, H.-I Yeh and S. Rothery. Annual of Cardiac
Surgery, 9th edition, 31-44, (1996).
7-Simultaneous Localization of Connexins 40, 37 and 43 in Pulmonary Artery
Endothelial Gap Junctions.
Y. S. Ko, H-I. Yeh, S. Rothery, E. Dupont, N. J. Severs. In: Proceedings 1997
International Gap Junction Conference, ISO Publishers, (1997).
8-Immunocytochemical Analysis of Connexin Expression in the Healthy and Diseased
Cardiovascular System.
N.J. Severs, S. Rothery, E. Dupont, S.R.Coppen, H-I. Yeh, Y-S.Ko, T. Matsushita, R.
Kaba, D. Halliday. Microsc. Res. Tech., 52, 301-322, (2001).
9- Re: The sinoatrial node, connexin distribution patterns and specific immunodetection
of connexin45.
S.R. Coppen, E. Dupont, N.J. Severs. Cardiovasc. Res., 53, 1043-5, (2002), author
reply.
10- Remodelling of gap junctions and connexin expression in heart disease.
N.J. Severs, E. Dupont, S.R. Coppen, D. Halliday, E. Inett, D. Baylis, S. Rothery.
Biochim. Biophys. Acta, 1662, 138-48, (2004).
11- Gap junction alterations in human cardiac disease.
N.J. Severs, S.R. Coppen, E. Dupont, H.I Yeh, Y.S. Ko, T. Matsushita. Cardiovasc Res.
62, 368-77, (2004).
12- Gap junction and connexin remodelling in human heart disease.
N.J. Severs, E. Dupont, R. Kaba, N Thomas. In: “Gap Junctions in Development and
Disease”. Edited by E. Winterhager, Springer-Verlag, Heidelberg, 57-83, (2005).
13- Alterations in cardiac connexin expression in cardiomyopathies.
N.J. Severs, E. Dupont, N. Thomas, R. Kaba, S. Rothery, R. Jain, K. Sharpey, C.H. Fry.
Adv Cardiol., 42, 228-42, (2006).
14- Gap junction channels and cardiac impulse propagation.
T. Desplantez, E. Dupont, N.J. Severs, R. Weingart., J Membr Biol., 218, 13-28, (2007).
15- Remodelling of gap junctions and connexin expression in diseased myocardium.
N.J. Severs, A.F. Bruce, E. Dupont, S. Rothery., Cardiovasc Res., 80, 9-19, (2008).

13. 13
Patent
UK Patent Application N0 9920277.2: “Diagnostic Method”. Describe the invention of a
diagnostic test to peri-operatively predict post-operative atrial fibrillation in patient
undergoing heart or heart and lung surgery.
E. Dupont, Y.-S. Ko, S. R. Coppen, M. P. Haw and N. J. Severs
Invited lectures
1- Gap Junctions and Structural Changes in Heart Failure.
Heart Failure 2001, 4th International Meeting of the Working Group of the European
Society of Cardiology. Barcelona, Spain, June 9-12th 2001.
2- Gap Junction Remodelling in Heart Failure.
24th Meeting of the European Section of the International Society for Heart Research.
Dresden, Germany, June 2d-6th 2004.
3- Gap Junction Remodelling in Heart Failure.
Lojda Symposium on Basic, Diagnostic and Applied Histochemistry. Kosice, Slovakia,
June 28th-July 1st 2004.
4- Connexins and Heart Failure.
3rd James Black Conference, British Pharmacological Society, Oxford, UK, September
18th- 20th 2005.
5- Structural Aspects of Gap Junctions.
Department of Physiology, University of Bern, Switzerland, October 18th 2006
6- Relating Connexin Co-expression and Connexon Structure to Cardiac Gap
Junction Function.
Wales Heart Research Institute, Cardiff University School of Medicine, Cardiff, UK, 26th
February 2007.
7- Relating Connexin Co-expression and Connexon Structure to Cardiac Gap
Junction Function.
The Swiss Cardiovascular Research and Training Network, Department of Physiology,
University of Bern, Switzerland, June 8th-9th 2007.
8- Relating connexin co-expression and connexon structure to cardiac gap
junction function.
Laboratorio de Investigación Cardiovascular, Servicio de Cardiología, Hospitals Vall
d'Hebron, Barcelona, Spain, June 17th 2008.
Oral communications, seminars, posters
My work from my PhD to now has been regularly presented in relevant international and
national meetings and in internal seminars. Over the last 10 years, I regularly attended the
ISHR meeting (average 3-4 abstracts), the international gap junction conference (average
5-6 abstracts), the national French gap junction conference and the UK gap junction
conference (both averaging 2-3 abstracts). My reputation in the cardiac gap junction field is
demonstrated by abstracts of the cell models of co-expression being selected for oral

14. 14
presentations at the International Gap Junction Conference by Dr N Thomas (Whistler,
Canada, summer 2005), Dr P Dias (Copenhagen, Denmark, summer 2007) and Dr RJ
Anicattu Issac (Ghent, Belgium, summer 2011).