Current Grades and Microscopes
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The document provides information to students about an upcoming microscope quiz, including the parts and proper use of microscopes. It details the different types of microscopes, like light and electron microscopes, and how to calculate magnification. The recap section has students label microscope parts in teams. The homework is to study the provided microscope information for the quiz tomorrow.
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Microscopes Electron microscope magnifies the specimen 1000X larger than the light microscopeThe Dissecting microscope on the other hand has a low magnification power (7X to 30X). It is used to view large objects
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Phase contrast
Darkfield
Polarizing
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Phase Contrast: illumination, sample contrast comes from interference of different path lengths of light through the sample.
labelsLight source, a light or a mirror (7)Diaphragm and condenser lens (8)Objective (3)Ocular lens (eyepiece) (1)In addition the vast majority of microscopes have the same 'structural' components:Objective turret/ nosepiece (to hold multiple objective lenses) (2)
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Field of view “FOV”the diameter of the circle of light that you see when looking into a microscope
Microscope (cont..)
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10X: the intermediate lens
40X: the high power lens
100X: the oil immersion lens
Microscope(cont..)
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Microscopy for
Microbiology – Use and
Function
Hands-On Labs, Inc.
Version 42-0249-00-02
Review the safety materials and wear goggles when
working with chemicals. Read the entire exercise
before you begin. Take time to organize the materials
you will need and set aside a safe work space in
which to complete the exercise.
Experiment Summary:
You will identify the components of an optical microscope.
You will describe field of view and depth of field. You
will view a series of preserved specimens including
protozoans, fungi, and bacteria. You will prepare wet
mounts of cheek cells and dental tartar.
EXPERIMENT
© Hands-On Labs, Inc. www.HOLscience.com 1
Learning Objectives
Upon completion of this laboratory, you will be able to:
● Outline the components of an optical microscope.
● Describe field of view and depth of field.
● Calculate the total magnification and field of view for the lenses of an optical microscope.
● Examine prepared slides under scanning, low, high, and oil immersion lenses.
● Prepare wet mounts of cheek cells and dental plaque and examine them under different
lenses.
Time Allocation: 3.5 hours
www.HOLscience.com 2 ©Hands-On Labs, Inc.
Experiment Microscopy for Microbiology - Use and Function
www.HOLscience.com 3 ©Hands-On Labs, Inc.
Experiment Microscopy for Microbiology - Use and Function
Materials
Student Supplied Materials
Quantity Item Description
1 Camera, digital or smartphone
1 Distilled water
1 Roll of paper towels
HOL Supplied Materials
Quantity Item Description
2 Blank slides
1 Pair of gloves
1 Lens paper (50 sheets)
1 Long thin stem pipet
1 Metric ruler
1 Safety goggles
1 Slide Box:
1 – Slide – Amoeba, whole mount
1 – Slide – Bacteria bacillus form
1 – Slide – Bacteria coccus form
1 – Slide – Bacteria spirillum
1 – Slide – Letter e focusing slide
1 – Slide – Penicillium with Conidia
1 – Slide – Yeast, whole mount
1 Slide cover glass cube
1 Sterile swabs, 2 per pack
1 Student microscope with 100x oil immersion lens*
1 - Immersion Oil
*Microscopes and oil immersion lens are purchased separately from the LabPaq kit.
Note: To fully and accurately complete all lab exercises, you will need access to:
1. A computer to upload digital camera images.
2. Basic photo editing software, such as Microsoft Word® or PowerPoint®, to add labels, leader
lines, or text to digital photos.
3. Subject-specific textbook or appropriate reference resources from lecture content or other
suggested resources.
Note: The packaging and/or materials in this LabPaq kit may differ slightly from that which is listed
above. For an exact listing of materials, refer to the Contents List included in your LabPaq kit.
www.HOLscience.com 4 ©Hands-On Labs, Inc.
Experiment Microscopy for Microbiology - Use and Function
Background
Optical Microscopes
A microscope is an optical instrument that uses a lens or a series of lenses to magnify objects that
are either too small to be seen ...
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This document provides an overview of basics of microscopy. It discusses obtaining a single field of view, color coding objectives, and microscope movements. It explains that the field of view is the circular area seen through the eyepiece, and total magnification is calculated by multiplying the objective and eyepiece powers. Objectives are color coded as red for 4X, yellow for 10X, blue for 40X, and white for 100X oil immersion. Specimens appear upside-down and backward under the microscope, and slide movements appear in the opposite direction of actual movement.
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1
Biol 1009 Lab Manual
NOTE: You must complete at least 7 out of 8 labs to pass this course.
The labs are not optional.
Contents
Lab Exercise
Page
1 Use of the Microscope
3
2 Enzyme Activity and Digestion of Carbohydrates and Proteins 13
3 Fermentation, Photosynthesis, and Respiration 19
4 The Structure of a Flower
28
5 Growth and Trophic Responses 31
6 Pedigree Tasting
39
7 Morphogenesis in Insects 44
8 The Ecological Community Structure 51
2
3
Lab Exercise 1: Use of the Microscope
As part of the assignment for this lesson, complete the following lab exercise.
Read through all of the lab exercise before you begin so that you’ll know what
you’re expected to do, what equipment to gather, and the questions you’ll need to
answer.
Write down your answers to the questions as you do the work. These are the
questions you will answer in your lab report. Go to the Assignments tool on the
course Web site to download the worksheet on which to enter your answers.
Timing note: A few hours, but you will need to arrange an appointment in advance.
NOTE: Most students use the microscope that is set up on the U of M campus (see
below). To avoid scheduling problems using this microscope, you do not have to
complete this lab until the end of week 3. However, sometimes a lot of students wait
until the end of week 3 to do this lab, so it’s best to schedule your microscope
appointment early (see below).
Arranging to Use a Microscope
If you live near the University of Minnesota, you may come to the St. Paul campus
and use a microscope in the College of Biological Sciences instructional
laboratories. To be sure a microscope is available for your use, please make an
appointment by contacting Sandy Mand at [email protected] or call 6126262821.
The preferred day and time for appointments is Friday, 9 a.m.–5 p.m., but other
days and times can usually be arranged. Also be sure to call this number if you
need to cancel your appointment.
If you do not live near campus, contact a local grade school, high school, college, or
laboratory to see if you may use one of their microscopes. Try to find some time that is
convenient to both you and the lending institution. Many institutions will be gracious
about letting you use their equipment on their premises.
It will take you approximately three hours to complete this lab exercise.
4
mailto:[email protected]
Objectives
In this exercise you will learn about the use and care of the compound light
microscope. You will use it to examine plant and animal materials, prepare your own
temporary slides, and make approximate measurements of viewed materials.
Supplies
If possible, borrow the following equipment:
● compound light microscope with scanning, low, and highpower objectives
● slide with three different colors of thread*
● slide of plant tissue*
● slide of anima.
Microscope handout-ek
This document provides instructions on how to use a compound light microscope. It describes the main parts of the microscope, including the eyepiece, objectives, stage, focus knobs, light source, frame, and base. It explains that total magnification is calculated by multiplying the objective and ocular magnifications. The document also outlines general procedures for carrying, setting up, focusing, and storing the microscope safely. Key steps include using the coarse focus to initially view the specimen, fine focusing for clarity, switching objectives while re-focusing, and adjusting the light as needed.
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Begin with the end in mind
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Seek first to understand, then to be understood
Synergise
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Desirable characteristics in a followerHonestCooperativeDependableCompetent
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What is a microscope?It is a tool or instrument often used in laboratories to aid in viewing organisms that cannot be seen with an unaided eye
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Biologist often used different microscope
Compound light microscope
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Zacharias Janssen is to be credited for inventing the first truly compound microscope
Microscope
What is a microscope?It is a tool or instrument often used in laboratories to aid in viewing organisms that cannot be seen with an unaided eye
Types of microscope
Biologist often used different microscope
Compound light microscope
Dissection microscope
Scanning electron microscope(SEM)
Transmission electron microscope(TEM)
but of all, the compound light microscope is what we shall be using
Dissecting Vs. Compound light microscope
Scanning Vs. Transmission electron Microscope
Microscope Compound light microscope is simply a microscope that has at least two magnifying lenses (the oculars and the objectives)Binocular microscope possess two eyepieces (what we shall be using in the lab)Monocular microscope possess one eyepieces
Bi = two mono = one
Microscopes Electron microscope magnifies the specimen 1000X larger than the light microscopeThe Dissecting microscope on the other hand has a low magnification power (7X to 30X). It is used to view large objects
*
Microscope There are a variety of light microscope but each differs by the emission of light through the specimen that is viewed
Phase contrast
Darkfield
Polarizing
UV
Terms Bright Field: illumination, sample contrast comes from absorbance of light in the sample.
Cross polarized light: illumination, sample contrast comes from rotation of polarized light through the sample.
TermsDark Field: illumination, sample contrast comes from light scattered by the sample.Working distance: distance between the specimen and the objective lens ( is this distance greater with the 40x or the 10x objective ? Ans: 10x )
Phase Contrast: illumination, sample contrast comes from interference of different path lengths of light through the sample.
labelsLight source, a light or a mirror (7)Diaphragm and condenser lens (8)Objective (3)Ocular lens (eyepiece) (1)In addition the vast majority of microscopes have the same 'structural' components:Objective turret/ nosepiece (to hold multiple objective lenses) (2)
LabelsStage (to hold the sample) (6)Focus wheel to move the stage (4 - coarse adjustment, 5 - fine adjustment) Stage adjustment knobs (9)
Terms used when dealing with a microscope
The headIt supports the two sets of magnifying lenses
The Oculars (eyes)It is the lenses used to view the specimen.Located on the eyepieceHas a magnification of 10X
Microscope
Interpupillary distanceThis is the distance between the eyepiece on a binocular microscopeIt can be adjusted to suit your eyes
Field of view “FOV”the diameter of the circle of light that you see when looking into a microscope
Microscope (cont..)
ObjectivesThese are the four lenses on the revolving nosepieceFour types exist: 4x , 10x , 40x , 100x
4X : the scanning lens
10X: the intermediate lens
40X: the high power lens
100X: the oil immersion lens
Microscope(cont..)
The ArmThe part ...
Microbiology (lab report 1 format)
The document provides instructions for students on the proper use and care of a microscope during laboratory experiments. It describes the main components of the microscope, including the body, base, stage, illumination system, magnifying lenses, and adjusting system. It provides step-by-step instructions on how to use the different objective lenses, adjust the light and focus to view unstained and stained samples. Students are advised to clean lenses after use and store the microscope properly.
Microscopy for Microbiology – Use and FunctionHands-On L.docx
Microscopy for
Microbiology – Use and
Function
Hands-On Labs, Inc.
Version 42-0249-00-02
Review the safety materials and wear goggles when
working with chemicals. Read the entire exercise
before you begin. Take time to organize the materials
you will need and set aside a safe work space in
which to complete the exercise.
Experiment Summary:
You will identify the components of an optical microscope.
You will describe field of view and depth of field. You
will view a series of preserved specimens including
protozoans, fungi, and bacteria. You will prepare wet
mounts of cheek cells and dental tartar.
EXPERIMENT
© Hands-On Labs, Inc. www.HOLscience.com 1
Learning Objectives
Upon completion of this laboratory, you will be able to:
● Outline the components of an optical microscope.
● Describe field of view and depth of field.
● Calculate the total magnification and field of view for the lenses of an optical microscope.
● Examine prepared slides under scanning, low, high, and oil immersion lenses.
● Prepare wet mounts of cheek cells and dental plaque and examine them under different
lenses.
Time Allocation: 3.5 hours
www.HOLscience.com 2 ©Hands-On Labs, Inc.
Experiment Microscopy for Microbiology - Use and Function
www.HOLscience.com 3 ©Hands-On Labs, Inc.
Experiment Microscopy for Microbiology - Use and Function
Materials
Student Supplied Materials
Quantity Item Description
1 Camera, digital or smartphone
1 Distilled water
1 Roll of paper towels
HOL Supplied Materials
Quantity Item Description
2 Blank slides
1 Pair of gloves
1 Lens paper (50 sheets)
1 Long thin stem pipet
1 Metric ruler
1 Safety goggles
1 Slide Box:
1 – Slide – Amoeba, whole mount
1 – Slide – Bacteria bacillus form
1 – Slide – Bacteria coccus form
1 – Slide – Bacteria spirillum
1 – Slide – Letter e focusing slide
1 – Slide – Penicillium with Conidia
1 – Slide – Yeast, whole mount
1 Slide cover glass cube
1 Sterile swabs, 2 per pack
1 Student microscope with 100x oil immersion lens*
1 - Immersion Oil
*Microscopes and oil immersion lens are purchased separately from the LabPaq kit.
Note: To fully and accurately complete all lab exercises, you will need access to:
1. A computer to upload digital camera images.
2. Basic photo editing software, such as Microsoft Word® or PowerPoint®, to add labels, leader
lines, or text to digital photos.
3. Subject-specific textbook or appropriate reference resources from lecture content or other
suggested resources.
Note: The packaging and/or materials in this LabPaq kit may differ slightly from that which is listed
above. For an exact listing of materials, refer to the Contents List included in your LabPaq kit.
www.HOLscience.com 4 ©Hands-On Labs, Inc.
Experiment Microscopy for Microbiology - Use and Function
Background
Optical Microscopes
A microscope is an optical instrument that uses a lens or a series of lenses to magnify objects that
are either too small to be seen ...
Microbiology1 cells & processes
1. The document discusses the key characteristics and structures of cells, including their components like the cell membrane, nucleus, chloroplasts, and mitochondria.
2. It explains the differences between prokaryotic and eukaryotic cells, and describes the structures of plant and animal cells.
3. The document also covers cellular metabolism, the four main macromolecules (carbohydrates, lipids, proteins, nucleic acids), and how cells obtain energy from breaking down food molecules.
Basics of microscopy
This document provides an overview of basics of microscopy. It discusses obtaining a single field of view, color coding objectives, and microscope movements. It explains that the field of view is the circular area seen through the eyepiece, and total magnification is calculated by multiplying the objective and eyepiece powers. Objectives are color coded as red for 4X, yellow for 10X, blue for 40X, and white for 100X oil immersion. Specimens appear upside-down and backward under the microscope, and slide movements appear in the opposite direction of actual movement.
Microscopy 1
The document provides information about microscopy, including:
1. Defining key microscope terms like magnification, resolving power, contrast, and depth of field.
2. Describing the parts and proper use of compound light microscopes, including objectives, oculars, stages, condensers, and focusing.
3. Explaining how microscope images are inverted and techniques like using different magnifications, iris diaphragms, and immersion oil.
Lecture 3 Unit-I Bright-field & Dark-field Microscopy.pdf
The document provides information about bright field and dark field microscopy. It begins with an overview of different types of light microscopes including bright field, dark field, phase contrast and fluorescent. It then describes the principles, components and working of bright field microscopy, including sample illumination, contrast mechanisms, magnification and applications. Next, it discusses dark field microscopy, explaining how it works by blocking the light source and illuminating samples with scattered light so they appear bright against a dark background. It compares key differences between bright field and dark field microscopy and lists some common applications and advantages of dark field microscopy.
1 Biol 1009 Lab Manual NOTE You must complete at .docx
1
Biol 1009 Lab Manual
NOTE: You must complete at least 7 out of 8 labs to pass this course.
The labs are not optional.
Contents
Lab Exercise
Page
1 Use of the Microscope
3
2 Enzyme Activity and Digestion of Carbohydrates and Proteins 13
3 Fermentation, Photosynthesis, and Respiration 19
4 The Structure of a Flower
28
5 Growth and Trophic Responses 31
6 Pedigree Tasting
39
7 Morphogenesis in Insects 44
8 The Ecological Community Structure 51
2
3
Lab Exercise 1: Use of the Microscope
As part of the assignment for this lesson, complete the following lab exercise.
Read through all of the lab exercise before you begin so that you’ll know what
you’re expected to do, what equipment to gather, and the questions you’ll need to
answer.
Write down your answers to the questions as you do the work. These are the
questions you will answer in your lab report. Go to the Assignments tool on the
course Web site to download the worksheet on which to enter your answers.
Timing note: A few hours, but you will need to arrange an appointment in advance.
NOTE: Most students use the microscope that is set up on the U of M campus (see
below). To avoid scheduling problems using this microscope, you do not have to
complete this lab until the end of week 3. However, sometimes a lot of students wait
until the end of week 3 to do this lab, so it’s best to schedule your microscope
appointment early (see below).
Arranging to Use a Microscope
If you live near the University of Minnesota, you may come to the St. Paul campus
and use a microscope in the College of Biological Sciences instructional
laboratories. To be sure a microscope is available for your use, please make an
appointment by contacting Sandy Mand at [email protected] or call 6126262821.
The preferred day and time for appointments is Friday, 9 a.m.–5 p.m., but other
days and times can usually be arranged. Also be sure to call this number if you
need to cancel your appointment.
If you do not live near campus, contact a local grade school, high school, college, or
laboratory to see if you may use one of their microscopes. Try to find some time that is
convenient to both you and the lending institution. Many institutions will be gracious
about letting you use their equipment on their premises.
It will take you approximately three hours to complete this lab exercise.
4
mailto:[email protected]
Objectives
In this exercise you will learn about the use and care of the compound light
microscope. You will use it to examine plant and animal materials, prepare your own
temporary slides, and make approximate measurements of viewed materials.
Supplies
If possible, borrow the following equipment:
● compound light microscope with scanning, low, and highpower objectives
● slide with three different colors of thread*
● slide of plant tissue*
● slide of anima.
Microscope handout-ek
This document provides instructions on how to use a compound light microscope. It describes the main parts of the microscope, including the eyepiece, objectives, stage, focus knobs, light source, frame, and base. It explains that total magnification is calculated by multiplying the objective and ocular magnifications. The document also outlines general procedures for carrying, setting up, focusing, and storing the microscope safely. Key steps include using the coarse focus to initially view the specimen, fine focusing for clarity, switching objectives while re-focusing, and adjusting the light as needed.
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Current Grades and Microscopes
- 1. Daily Stimulus Input grades for Quiz 1
- 2. Current Grades
- 4. HW Check: Pg 51 “5 Types of Cells”
- 6. How to use a Microscope
- 7. “ Stand Up, Hand Up, Pair Up”
- 8. HW: Study for Microscope Quiz
- 10. SWBAT explain how to use a microscope
- 12. GLO#6: Effective and ethical users of technology
- 14. Everyone must get 100% to be able to use the microscope!
- 15. If you pass the quiz, paste it to page 64
- 19. Electron microscope: Uses electrons to magnify up to 1,000,000 times!! Samples must be dead.
- 22. Red Objective: 4x
- 23. TOTAL MAGNIFICATION: 40x
- 25. Yellow Objective: 40x
- 26. TOTAL MAGNIFICATION: 100x Magnification
- 28. Blue Objective: 40x
- 29. TOTAL MAGNIFICATION: 400x
- 30. Lets Recap...
- 32. Team statement: Microscope Rule