curcumin eye drop preparation trial

1. Aim- To prepare hydroalcoholic eye drop of curcumin.
References-
1. De PK, Dhibar M, Rakshit S. Fabrication and characterization of Curcumin Nanoemulgel to
Overcome the Challenges in Opthalmic Drug Delivery System. 2018;4929.
doi:10.21276/sjmps.2018.4.1.14
2. Nair KA, Somashekara N, Venkata M, et al. Development of Curcumin Based Ophthalmic
Formulation Research and Development , Laila Pharmaceuticals Pvt . Ltd ., Chennai ,
Tamilnadu , India Research and Development , Laila Impex-R and D Centre , Vijayawada
andhra Pradesh , India P . D . Patel Institute of Applied Sciences , Charotar University of
Science and Technology , Department of Biotechnology , Indian Institute of Technology-
Madras , Chennai , India. 2012;8(1):41-49.
3. Noh G, Keum T, Seo J, Choi J, Rakesh B. Development and Evaluation of a Water Soluble
Fluorometholone Eye Drop Formulation Employing Polymeric Micelle.2018.
doi:10.3390/pharmaceutics10040208.
4. Davis BM, Pahlitzsch M, Guo L, et al. Topical Curcumin Nanocarriers arNeuroprotective in Eye
Disease. Sci Rep. 2018;(March):1-13. doi:10.1038/s41598-018-29393-8.
Requirements-
Chemicals-
1. Pure 1 g of Curcumin
2. Ethanol
3. Distilled water
Apparatus-
1. Beaker, Weighing balance
2. Pipette, Test tubes etc.
Procedure-
1. Clean all apparatus accurately
2. Accurately weight 2mg of curcumin and added into 6 mL of ethanol in test tube then
shake the solution. Make up the volume upto 10 mL by adding Distilled water.
3. Take 1 mL from above solution in another test tube and make up the volume upto 10 mL
by adding Distilled water.
4. Again take 1 mL from above solution in another test tube and make up the volume upto
10 mL by adding Distilled water. By using above procedure make further 3 dilution so, 5
dilutions was prepared.
Preparation of Curcumin Eye Drop
Experiment No.1

2. Calculation-
Dilutions code Concentration of Drug Content %
A 20%
B 2%
C 0.2%
D 0.02%
E 0.002%
Dilution Factor- 10 for dilution A: 2/10 X 100=20%
Result image-

3. Procedure-
1. Clean all apparatus accurately
2. Accurately weight 5mg of curcumin and added into 3 mL of ethanol in test tube then
shake the solution. Make up the volume upto 10 mL by adding Distilled water.
3. Take 1 mL from above solution in another test tube and make up the volume upto 10 mL
by adding Distilled water.
4. Again take 1 mL from above solution in another test tube and make up the volume upto
10 mL by adding Distilled water. By using above procedure make further 3 dilution so, 5
dilutions was prepared.
Calculation-
Dilutions code Concentration of Drug Content %
A 50%
B 5%
C 0.5%
D 0.05%
E 0.005%
Dilution Factor- 10 for dilution A: 5/10 X 100=50%
Result image-
Experiment No.2