This document summarizes various tests conducted on packaging materials, including leakage tests, collapsibility tests, clarity tests of aqueous extracts, water vapor permeability tests, transparency tests, and biological tests. The biological tests examine the effects of packaging extracts on mice and rabbits through injection, skin application, and eye irritation tests to check for any adverse biological activities. Additional tests ensure the packaging can withstand stresses like temperature variations and force without breaking or deteriorating. Overall, the document outlines a series of tests used to evaluate the safety and durability of packaging materials.

1. By: Dr. Kavita Bahmani
Assistant Professor
GJUS&T, Hisar, Haryana, India

2. 1. Leakage Test
2. Collapsibility Test
3. Clarity Aqueous Extract
4. Water vapour permeability test
5. Transparency Test
6. Biological Test
a. Systemic injection test
b. Intra cutaneous Test
c. Eye Irritation Test on rabbits
d. Resistance to stress
e. Resistance to temperature variation

3. Leakage Test
Fill the container with water
Fit with intended closure and keep them inverted at room
temp. for 24 hrs.
Test is passed if no sign of leakage from container

4. Collapsibility Test
 Applicable to containers which are squeezed
for removing the contents
 A container by collapsing inward during
use, yield at least 90% of its normal
contents at the required rate of flow at
ambient temperature

5. Clarity Aqueous Extract
Random selection of unlabeled, unmarked and non
laminated portions from suitable containers
Cut in strips with total areas 20 sq.cm. Wash the
strips for 30 seconds & drain off the water
The sample is then taken into flask, previously
cleaned with chromic acid and rinsed with distilled water
several times
Then to flask with sample, 250 ml distilled water is added
and autoclave at 121 ͦC for 30 min
Same should be done for blank on examination
the extract should be colorless & free from turbidity

6. Water vapour permeability test
Fill 5 containers with normal vol. of water and heat seal
the bottles with aluminum foil.
Weigh accurately each container and allowed to stand
for 14 days at relative humidity of 60±5% & temp. 20-25ͦC.
Reweight the container the loss in weight in each
container is NMT 0.2%

7. Transparency test
Standard suspension preparation:
1 gm. hydrazine sulphate in 100 ml water and set aside
for 6 hr. Take 25 ml. of this solution and add 25 ml. of
10% w/v hexamine solution and stand for 24 hrs.
Test solution preparation:
Sample is prepared by 16 fold dilution of the standard
suspension
Fill the containers & cloudiness detectable when
compared to a water filled containers. Absorbance is
measured at 640 nm and range is within 0.37 to 0.43

8. Biological Tests
1. Systemic injection test:
The test is perform on albino mice. In this 5 mice in each
groups i.e test and blank are taken . The sample is inject
in mice and observe for any significant biological
activities for 4 hrs, 24 hrs, 48 hrs & 72 hrs. if abnormal
behaviour such as convulsions & prostrations r loss in
body weight (˂2 g), the sample does not meet the
requirements.
2. Intra cutaneous test:
This test is perform of rabbits. In this reactions like
erythema, edema & neurosis are studied for 24, 48, 72 hrs.

9. 3. Eye Irritation test:
This test is perform on rabbits. Any irritation in eyes
due to sample is studied as compared to blank
4. Resistance to stress:
In this the water is acidified with 2M HCL. The acidified
water is filled in container and apply force to both end
of containers. No break, or deterioration should occur
for the sample to pass the test.

10. THANK YOU