The document describes a test system for rapid diagnosis of pregnancy in livestock. The system allows diagnosis through a liquid or test strip using urine samples and can detect pregnancy from 2 weeks onwards without specialized equipment. It provides reliable results (99.2-99.5% accuracy) within 30 minutes. Compared to existing methods like rectal exams or laboratory tests, the system requires no veterinarian, costs less at 2-3.5 euros per test, and poses no infection risk. Market analysis shows strong domestic and export demand that could reach 6,500 and 3,600 units annually by 2016. The producer is seeking cooperation on sales, production and financing to develop the innovative technology.

1. TTest system for rapidest system for rapid
diagnosis of pregnancydiagnosis of pregnancy
livestocklivestock
«Scientific-Technical Center «PromTehEnergo», LLC

2. OObjective of the projectbjective of the project
Creating a production test systems in the form of aCreating a production test systems in the form of a
liquid or a test strip for rapid diagnosis of immuneliquid or a test strip for rapid diagnosis of immune
pregnancy livestock (pigs, goats, sheep, cows, horses,pregnancy livestock (pigs, goats, sheep, cows, horses,
camels) in cattle farms and private holdings withoutcamels) in cattle farms and private holdings without
any special equipment, and without invitingany special equipment, and without inviting
specialists.specialists.

3. Technology for producing test systems in the formTechnology for producing test systems in the form
of liquid, and test strips, based on the determinationof liquid, and test strips, based on the determination
of placental hormones - human chorionicof placental hormones - human chorionic
gonadotropin qualitative detection in the urine ofgonadotropin qualitative detection in the urine of
animals of similar domestic pregnancy tests used inanimals of similar domestic pregnancy tests used in
medicine.medicine.
Technology necessarily involves obtainingTechnology necessarily involves obtaining
hyperimmune serum containing specifichyperimmune serum containing specific
antihoriogonin - antibodies.antihoriogonin - antibodies.
Diagnostic methodDiagnostic method

4. Technology for manufacturing test systemsTechnology for manufacturing test systemsStage1Stage1
Obtaining hyperimmune serumObtaining hyperimmune serum
36 days
Immunization with a 5-fold with a 7-day intervals (29 days)
Blood sampling method of total bleeding (7 days after the
last injection)
Determination of serum titer of hyperimmune
Stage2Stage2
Labeling antibodiesLabeling antibodies
antihoriogoninantihoriogonin
20.5 hours
Getting the suspension of fine particles of alizarin
(4 hours and 45 minutes)
Adsorption of antibodies on the particles antihoriogonin
indicator alizarin (15 hours 45 minutes)
Quality control of test systemsQuality control of test systems
Determination of the organoleptic and physical properties
(color, odor, texture)
The study of immunological specificity
Stage3Stage3

5. Fig. 1 - Tubes of urine prior to theFig. 1 - Tubes of urine prior to the
introduction of the reagentintroduction of the reagent
A - negative testA - negative test
B - positive testB - positive test
АА BB АА BB
Fig. 2 - The introduction ofFig. 2 - The introduction of
the reagentthe reagent
A - negative testA - negative test
B - positive testB - positive test
PPregnancy diagnosis by means of a test systemregnancy diagnosis by means of a test system
Fig. 3 - The contents of tubes to the mixingFig. 3 - The contents of tubes to the mixing
A - negative testA - negative test
B - positive testB - positive test
АА BB
АА BB
Fig. 4 - The contents of test tubes after mixingFig. 4 - The contents of test tubes after mixing
A - negative testA - negative test
B - positive testB - positive test

6. Fig. 5 - reading of the reaction after 15 minutesFig. 5 - reading of the reaction after 15 minutes
A - negative testA - negative test
B - positive testB - positive test
АА BB
АА BB
Fig. 6 - reading of the reaction after 40 minutesFig. 6 - reading of the reaction after 40 minutes
A - negative testA - negative test
B - positive testB - positive test
Fig. 7 - reading of the reaction after 60 minutesFig. 7 - reading of the reaction after 60 minutes
A - negative testA - negative test
B - positive testB - positive test
АА BB АА BB
Fig. 8 - reading of the reaction after 90 minutesFig. 8 - reading of the reaction after 90 minutes
A - negative testA - negative test
B - positive testB - positive test
PPregnancy diagnosis by means of a test systemregnancy diagnosis by means of a test system

7. Technical and functional characteristics of test systemsTechnical and functional characteristics of test systems
•Consumers formConsumers form – liquid or test strip;
•Convenience and ease of use in livestock farms and private
holdings without using specialized equipment;
•The degree of conformity in the diagnosis of the presence orThe degree of conformity in the diagnosis of the presence or
absence of pregnancy in animalsabsence of pregnancy in animals: at least 99.2 - 99.5%;
•Duration of diagnostic proceduresDuration of diagnostic procedures - no more than 0.5 hours;
•The term of the pregnancy, which can be a diagnosis onThe term of the pregnancy, which can be a diagnosis on
pregnancy and infertilitypregnancy and infertility: from 0.5 months;
•Ability to visually establish the positive or negative reaction
(reaction of clarity);
•Safety procedures for diagnosis of pregnancy and animals to
prevent the possibility of human infection diseases of infectious
origin.

8. Name of indicatorsName of indicators
The valuesThe values
TheThe
proposedproposed
test systemtest system
Domestic and foreign objects of similar purposeDomestic and foreign objects of similar purpose ForecastForecast
for 2012 - 2015for 2012 - 2015
yearsyears
The rectalThe rectal
methodmethod
radioimmunoaradioimmunoa
ssay testssay test
"Progitor""Progitor"
(Farmos(Farmos
DiagnosticaDiagnostica
(Finland)(Finland)
"Steron -"Steron -
PM-I-PM-I-
125"125"
(Ukraine)(Ukraine)
BioPRYBioPRY
NN
(BioTrac(BioTrac
king,king,
LLCLLC
((СШАСША))
The degree of conformity inThe degree of conformity in
the diagnosis of thethe diagnosis of the
presence or absence ofpresence or absence of
pregnancy in animalspregnancy in animals
(diagnostic efficiency)(diagnostic efficiency)
Duration of the reactionDuration of the reaction
diagnosisdiagnosis
The term of the pregnancy,The term of the pregnancy,
which can be a diagnosis onwhich can be a diagnosis on
pregnancy and infertilitypregnancy and infertility
Ability to visually establishAbility to visually establish
the positive or negativethe positive or negative
reaction (reaction of clarity)reaction (reaction of clarity)
not less thannot less than
99.2 - 99.5%99.2 - 99.5%
less than 15less than 15
minutesminutes
ranging fromranging from
2 weeks2 weeks
YesYes
95 – 100%95 – 100%
nono
3 - 43 - 4
monthsmonths
NoNo
96 – 99%96 – 99%
1,5 – 21,5 – 2
minutesminutes
20 - 25 days20 - 25 days
afterafter
inseminationinsemination
YesYes
85%85%
Not moreNot more
than 1.5than 1.5
hourshours
22 days after22 days after
inseminatioinseminatio
nn
YesYes
90%90%
Not moreNot more
than 2than 2
hourshours
21 days21 days
afterafter
inseminatiinseminati
onon
YesYes
95 – 99%95 – 99%
Not moreNot more
than 2than 2
hourshours
21 days21 days
afterafter
inseminainsemina
tiontion
YesYes
Conservation ofConservation of
diagnosticdiagnostic
performance at 98-performance at 98-
99,5%99,5%
Reducing the timeReducing the time
diagnosisdiagnosis
Reducing theReducing the
period ofperiod of
pregnancy, wherepregnancy, where
possible diagnosispossible diagnosis
on pregnancy andon pregnancy and
infertilityinfertility
Comparison with the methods and preparations of the same

9. Name of indicatorsName of indicators
The valuesThe values
TheThe
proposedproposed
test systemtest system
Domestic and foreign objects of similar purposeDomestic and foreign objects of similar purpose ForecastForecast
for 2012 - 201for 2012 - 20166
yearsyears
The rectalThe rectal
methodmethod
radioimmunoaradioimmunoa
ssay testssay test
"Progitor""Progitor"
(Farmos(Farmos
DiagnosticaDiagnostica
(Finland)(Finland)
"Steron -"Steron -
PM-I-PM-I-
125"125"
(Ukraine)(Ukraine)
BioPRYBioPRY
NN
(BioTrac(BioTrac
king,king,
LLCLLC
((СШАСША))
The presence of aThe presence of a
specialized laboratory forspecialized laboratory for
the diagnosisthe diagnosis
The need to involve aThe need to involve a
specialist - a veterinarianspecialist - a veterinarian
The cost of diagnosticThe cost of diagnostic
procedures,procedures, the euro.the euro.
Safety procedures forSafety procedures for
diagnosis of pregnancy anddiagnosis of pregnancy and
infertility of cows, for cowsinfertility of cows, for cows
and for veterinary specialist,and for veterinary specialist,
to prevent the possibility ofto prevent the possibility of
infection diseases specialistsinfection diseases specialists
of infectious origin.of infectious origin.
nono
nono
3,753,75
yesyes
yesyes
yesyes
2 – 52 – 5
nono
yesyes
yesyes
5,5 – 75,5 – 7
yesyes
yesyes
yesyes
5 – 65 – 6
yesyes
yesyes
yesyes
4,5 – 6,54,5 – 6,5
yesyes
yesyes
yesyes
5,5 – 7,55,5 – 7,5
yesyes
Comparison with the methods and preparations of the same

10. The test system for rapid diagnosis of immune pregnancy in the early
stages of livestock (pigs, goats, sheep, cows, horses, camels), the
aggregate of the above technical and economic characteristics and
functional properties of a compelling competitive advantages compared to
existing diagnostic methods and tests , namely:
A) the laboratory radioimmunoassay (RIA) and enzyme immunoassay
(ELISA) tests;
B) The rectal method;
B) foreign kits for enzyme immunoassay (EIA) and tests (Progestassay,
AccufirmTM and RPTTM (Kamar Inc., Steamboat Springs Co); RPT (Gist -
Brocades Inc., Charlotte, NC), BESTTM (West Agro Inc. Kansas Sity ,
MO); CalfcheckTM (Noctech Ltd., Dublin, Ireland); EnzygnostTM
(Hoechst-Roussel Agrivet Comp., Summerville, NJ); EstruCHEKTM,
OvuSureTM (Elanco Products Co., Indianapolis, IN); Open AlertTM and
BoviPro 21TM (An - Tech International, Proenix, AD); TargetTM
(BioMetallics, Princeton, NJ); «Progitor" firm Farmos Diagnostica
(Finland)), BioPRYN company BioTracking, LLC (USA).
Comparison with the methods and preparations of the same

11. The market in the first place, the European and American (U.S.,
Canada), and Russian markets, the markets of CIS countries.
Industry-consuming products: livestock (cattle, sheep, pig, horse,
camel), veterinary medicine.
The main consumers of the product:
- Foreign and domestic farmingForeign and domestic farming on a commercial basis involved in
breeding pigs, goats, sheep, cows, horses and camels;
- Foreign and domestic private ownersForeign and domestic private owners of livestock involved in
breeding pigs, goats, sheep, cows, horses and camels for their
personal use and to a lesser extent, to implement.
Market of test systemsMarket of test systems

12. Existing and projected demandExisting and projected demand
IndexIndex
YearYear
20120122 20120133 20120144 20120155 20120166
Volume of salesVolume of sales
test systems in the domestictest systems in the domestic
market:market:
in kind, thousand units.in kind, thousand units.
340340 760760 1 7001 700 3 8003 800 6 5006 500
in monetary terms,in monetary terms,
thousand euros.thousand euros. 650650 1 4101 410 3 1353 135 7 0657 065 12 09012 090
The share of the domesticThe share of the domestic
market,%market,%
0,90,9 1,91,9 4,44,4 9,79,7 16,716,7
Volume of salesVolume of sales
test systems in the foreigntest systems in the foreign
market:market:
in kind, thousand units.in kind, thousand units.
5050 240240 850850 1 9001 900 3 6003 600
in monetary terms,in monetary terms,
thousand euros.thousand euros. 170170 835835 2 9602 960 6 6256 625 12 55012 550
The share of foreign markets,The share of foreign markets,
%%
0,0020,002 0,10,1 0,40,4 0,90,9 1,61,6
Assess the demand for test systems is given to the cost of the 1st test system at a rate of 2 euros for a test on the Russian market
and 3.5 euros in foreign markets (EU, U.S., Canada).
[1] Start of the production was launched in May 2012.

13. Stage of the projectStage of the project
• Conducted research projects, developed and patentedConducted research projects, developed and patented
technology for manufacturing test systemstechnology for manufacturing test systems
• Marketing researchMarketing research
• Carried out development work, bought the requiredCarried out development work, bought the required
technological equipmenttechnological equipment
• Ongoing certification of products in accordance withOngoing certification of products in accordance with
Russian legislationRussian legislation
• Organization carried out pilot productionOrganization carried out pilot production

14. Options for cooperationOptions for cooperation
• Organization of sales in foreign marketsOrganization of sales in foreign markets
• Joint productionJoint production
• Joint participation in special orientedJoint participation in special oriented
programs and tenders to support andprograms and tenders to support and
finance the innovative productionfinance the innovative production
development.development.

15. Thank you for your attention!Thank you for your attention!
«Scientific-Technical Center «PromTehEnergo»,«Scientific-Technical Center «PromTehEnergo», LLCLLC
http://www.cowtest.ruhttp://www.cowtest.ru
mail.cowtest@gmail.commail.cowtest@gmail.com