1. The document discusses mixed conditional sentences, which combine elements of type II and type III conditional structures. It provides examples of different combinations, such as a type III conditional in the if-clause and a type II conditional in the main clause.
2. Mixed conditionals are used to describe hypothetical present or future situations that result from past events that did not actually happen. They allow for discussing how circumstances could be different based on changing past actions.
3. The most common mixed conditional structures are those that discuss a past action with a present result, a present condition with a past result, or a future action with a past result.
English conditional sentences 1st,2nd,3rd, and mixed conditional. Definition and examples of each. And also their use in daily life examples. Structure of forming sentences how can we write them.
What are Conditional Sentences
Why we need Conditionals
Grammatically
Present Real Conditional (Zero)
Future Real Conditional (1st)
Present + Future Unreal Conditional (2nd)
Past Unreal Conditional (3rd)
Errors
All together
Modals Verbs
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
English conditional sentences 1st,2nd,3rd, and mixed conditional. Definition and examples of each. And also their use in daily life examples. Structure of forming sentences how can we write them.
What are Conditional Sentences
Why we need Conditionals
Grammatically
Present Real Conditional (Zero)
Future Real Conditional (1st)
Present + Future Unreal Conditional (2nd)
Past Unreal Conditional (3rd)
Errors
All together
Modals Verbs
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Richard's entangled aventures in wonderlandRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Seminar of U.V. Spectroscopy by SAMIR PANDASAMIR PANDA
Spectroscopy is a branch of science dealing the study of interaction of electromagnetic radiation with matter.
Ultraviolet-visible spectroscopy refers to absorption spectroscopy or reflect spectroscopy in the UV-VIS spectral region.
Ultraviolet-visible spectroscopy is an analytical method that can measure the amount of light received by the analyte.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
3. Conditionals
Sometimes the two parts of a conditional
sentence refer to different times. This is
called a mixed conditionalre sentences
that combine the second and third
conditional together.. Ready to learn?
4. The main clause usually has the verb will / shall.
But we can also use:
Going to:
If Helen passes her exams, I'm going to buy her a present.
Future continuous:
If we book this holiday, we'll be lying on the beach in two weeks` time.
Future perfect:
If they win the next match, they'll have won every match in the season.
Imperative:
If you need me, call me.
Can / could, may / might, should / ought
to, must
If you don't eat your dinner, you can't have an ice cream.
5. The main clause usually has the modal would +
infinitive. However, the modals could and might are also
used and the infinitive can be in the continuous form:
If we had more money, we wouldn`t be living here.
If we had more money, we could go out more.
If you asked Ali, he might do it for you.
6. The main clause usually has the modal would +
perfect infinitive. However, we can also use the
modals could and might + perfect infinitive.
If I had known there was no more work to do, I
could have stayed in bed.
If the ambulance had come sooner, he might
have been saved.
7. Zero Conditional
• Uses: Use the zero conditional to
talk about scientific facts, constant
laws of nature, unchangeable rules,
customs and personal routines.
• Formula: present simple, present
simple
• Positive Example: If/When it is
lunch time in London, it is
breakfast time in New York.
• Negative Example: If/When it
is not below zero degrees Celsius, it
is not snowing.
• Notes: Either if or when can be
used in the if-clause.
First Conditional
• Uses: Use the first conditional to
talk about probable/possible
conditions in the future, or for
threats or warnings involving direct
action.
• Formula: present simple, future
• Positive Example: If it rains
tomorrow, I will bring my umbrella.
• Negative Example: If you
don´t like a food, don´t eat it.
• Notes: You can use an imperative
in the main clause
You can use the present continuous or
present perfect instead of the present
simple.
8. Second Conditional
• Uses: Use the second conditional to talk
about unreal or hypothetical situations in
the present or improbable events in the
future.
• Formula: past simple, would / wouldn´t
+ infinitive
• Positive Example: If I met Obama, I
would ask for his autograph.
• Negative Example: If I didn´t have a
small house, I would invite you all to a
party.
• Notes: You can use the past continuous
instead of the past simple.
• Instead of would, you can use could or
might.
Third Conditional
• Uses: Use the third conditional to talk
about past events that are untrue.
• Formula: past perfect, would have /
wouldn’t have + past participle
• Positive Example: If you had paid
attention, you would have known what to
do.
• Negative Example: If they had not
been looking the other way, they would
have seen the sign.
• Notes: You can use the past perfect
continuous instead of the past perfect.
9. Tense variations in conditional 2
The if clause usually takes the past simple. However, it is also
possible to use:
Past continuous:
If you were coming with me, I`d give you a lift.
Could
If I could have the day off, I`d go with you.
Were / was to:
If you were to ask Steve, I`m sure he would do it.
10. Tense variations in conditional 3
The if clause is usually in the past perfect simple.
However, we can also use the past perfect
continuous or could + perfect infinitive.
If he had been travelling in the car, he would
have been killed too.
If we could have got a taxi, we would have
come round to see you.
11. Words other than if
Unless I won`t go out unless he comes too.
Even if Even if it rains, we`ll go to the match.
If only If only I had money, I would go out more.
As/so long as You can use the car as long as you take care of
it.
On condition
(that)
I`ll lend you the car on condition that you take
care of it.
Provided /
providing
(that)
I`ll lend you the car provided that you take care
of it.
Assuming
(that)
Assuming that you take care of the car, I`ll lend
it to you.
12. Suppose /
supposing (that)
Suppose you had a lot of
money, would you give up
work?
What if What if you had a lot of money,
would you give up work?
Imagine Imagine you had a lot of
money, would you give up
work?
In questions….
13. Mixed Conditionals
When we talk about mixed conditionals, we are referring to
conditional sentences that combine two different types of conditional
patterns.
14. What Are Mixed Conditionals?
Mixed conditionals are conditional sentences that combine elements
oftype II conditionalsandtype III conditionals. They are used to
describe ahypothetical or unreal situationin the present or
futurethat is connected to a hypothetical or unreal situationin the
past. In other words, mixed conditionals describe a present or future
situation that is the result of a past hypothetical event that did
notactuallyhappen.
17. MIXED CONDITIONALS
If-clauses canbemixedprovidedthattheymakesensewithina context.
If-clause Main clause
Type 3 If she had worked harder
at university,
She would have a degree
now.
Type 2
(she didn`t work hard at
university
so she doesn`t have a
degree now. )
Type 2 If I were faster, I would have won the
race.
Type 3
(I´m not fast so I didn´t win the race.)
Type2 If they were studying all
morning,
they will be tired now. Type 1
(they were studying so they are tired now.)
18. TimeandTense
Mixed conditionals are used to describe hypothetical situations that connect past, present, and future
events. They allow us to use different verb tenses to talk about actions that occur at different times.
It's important to note that while verb tense and time are often related, they are not always the same.
Forexample:
Sheworked atahospital.
Shewasworkingasanurse.
These two examples do not have the same tense, but they are both referring to the same time, which
ispast.
Mixedconditionals aresentences thatuse twodifferent times.Check outthe examples:
IfIhadslept earlier,Iwouldn'thavemissedmy appointment.
Both the 'if clause' and the 'main clause' have the same time: past. But they have different
tensesinthepast.
IfIhadslept earlier,Iwouldbeatmyappointment now.
19. Themost common combinations ofmixedconditionalsare:
Thistype ofmixedconditionalisusedtodescribeahypothetical presentor futureresultthatis
theconsequenceofapastactionthatdidnot actuallyhappen.Thistype ofmixedconditionaldeals
withchangingapastactionanditshypothetical present orfutureresult.However,it'simportant to
note thattheresultcannotactuallyhappen becausewecannotchangethepast.Checkout the
examples:
IfIhadfinishedhighschool,Iwouldbe acollege student now.
Hereinthisexample, thespeakerisnotacollegestudentnow,butheistalkingaboutachange
inthepastanditsresult.
Wewouldbegetting marriedifyou hadsaidyes.
Mixed Conditionals: Functions
1-PastActionwithPresent Result
20. 2-Present ConditionwithPastResult
The second type of mixed conditional is used to imagine a hypothetical
present situation that is the result of a past action that did not actually
happen.
Ifshe weremore hardworking,she wouldhave succeeded.
IfIweren't injured, Iwouldhave played.
21. This type of mixed conditional is used to describe a hypothetical situation in the present or
future that is the result of a past action that did not actually happen. It helps us to explore how the
present or future could be different if we had acted differently in the past.
Let's explain this function with an example. Imagine that you are a soccer player and tomorrow you
have an important match. You will probably stay home the night before the match, eat a healthy
dinner,andtryto bewell-rested. Soyoumightsay:
IfIdidn'thaveanimportant matchtomorrow, Iwouldhavestayed uplate.
Here, the person means: I am not staying up late because I have an important match
tomorrow.
IfIwasn'tparticipating inamatch,Iwouldhaveeatenicecreamafterdinner.
3-Future Actionwith PastResult
22. 3rd Conditional Mixed Conditional
Describes what the outcome
would have been in the past, if
things had happened differently
in the past.
Example: If Houda hadn’t given
birth to a unicorn, she wouldn’t
have gone to the hospital last
night.
• Describes what the outcome
would be in the present, if
things had happened
differently in the past.
• Example: If Houda hadn’t
given birth to a unicorn, she
wouldn’t be a mother today.
What is the difference between the third
conditional and the mixed conditional?
23. Mixed 3/2 Conditional
• 3rd conditional in the if-clause followed by
a 2nd conditional in the main clause
Example:
If he had taken the medication as prescribed, he
wouldn't still be sick in bed.
If Clause (3rd) Main Clause (2nd)
If + had/hadn’t +
past participle
Would/wouldn’t + infinitive
24. Mixed 3/2 Conditional
• With this combination we are describing what
the outcome would be in the present, if things
had happened differently in the past.
the opposite of what actually happened
Example: If she had taken reasonable
precautions, she wouldn't be pregnant now.
Hypothetical present outcome
25. Mixed 2/3 Conditional
• 2nd conditional in the if-clause followed by a
3rd conditional in the main clause
Example: If you weren´t so scary, you
wouldn’t have made the baby cry.
If clause (2nd) Main Clause (3rd)
If + simple past Would/wouldn’t + have +
past participle
26. Mixed 2/3 Conditional
• With this combination we are describing ongoing
circumstances or characteristics in relation to a past
event.
ongoing characteristic
Example: If you weren't such a poor dancer, you
would've got a job in the chorus line in that musical.
past event
27. Mixed Conditinal Practice
If you weren't so blind to his faults, you ________that he was out to swindle
you. (realize)
If you weren't so blind to his faults, you would've realized that he was out to
swindle you.
• If he hadn't run after the car thief and suffered a heart attack, he ___ (die).
• If he hadn't run after the car thief and suffered a heart attack, he wouldn't have
died.
• She wouldn’t need a visa to work here if she _______ (be) born in Europe.
• She wouldn’t need a visa to work here if she had been born in Europe.
• Sam would have translated the letter for you if he ______ (speak) Russian.
• Sam would have translated the letter for you if he spoke Russian.