The document summarizes an Indian laboratory's experience participating in the College of American Pathologists (CAP) external proficiency testing program for Luminex-based HLA antibody detection assays over 12 months in 2014. The laboratory processed CAP EQAS samples comprising plasma or whole blood samples and tested them for HLA class I and class II IgG antibodies using Luminex, as part of the laboratory's accreditation and quality assurance. Participating in the CAP EQAS program helped validate the laboratory's Luminex antibody testing for pre-transplant evaluation of patients with end-stage renal disease in India.