CLOSED SPAWNING SYSTEM METHOD OR CLOSED BREEDING FOR ARTIFICIAL PROPAGATION OF CLARIAS GARIEPINUS.pptx
1. CLOSED SPAWNING SYSTEM METHOD OR CLOSED
BREEDING FOR ARTIFICIAL PROPAGATION OF
CLARIAS GARIEPINUS
NOVEMBER, 2022
2. INTRODUCTION
Because there is no dependable way for propagating the species in industrial fish farms,
Clarias gariepinus rearing in Africa has not had much success throughout the years.
According to Sahoo (2016) catfish do not have the enabling environment (conditions)
necessary to complete their natural reproductive cycle in culture conditions. The catfish's
ability to undergo ultimate oocyte maturation and spawning is physiologically restricted by
these poor culture conditions (the absence of natural environmental cues). Therefore, in
captivity, the catfish will require the external hormone injection method that has been
utilized to trigger maturation and ovulation in economically significant fishes, including the
catfish, to induce its natural reproductive process.
3. JUSTIFICATION
According Akinrotimi et al. (2017), due to the rising need for fish as a source of protein around
the world over the past few decades, aquaculture has expanded tremendously The lack of high-
quality seed that is consistent in size and devoid of illnesses, parasites, and pests when stocked
in culture ponds is the single biggest impediment to the large-scale commercial culture of
various fish species. Odedeyi (2017) observed that in induced breeding, the largest mature
Clarias gariepinus would typically produce the best spawn weight, but there is little literature to
indicate if the fish producing the best spawn would also produce the best fry survival and
growth.In light of this, this study will evaluate the closed spawning system method or closed
breeding for artificial propagation of Clarias gariepinus.
4. AIM AND OBJECTIVES
Aim of the study
The aim of the study is to evaluate the closed spawning system method or closed
breeding for artificial propagation of Clarias gariepinus.
Objectives of the Study
Examine the fertilization rate of African cat fish (Clarias gariepinus) under closed
spawning system method of artificial propagation.
Examine the incubation period of African cat fish (Clarias gariepinus) under
closed spawning system method of artificial propagation.
Determine the hatchability rate of African cat fish (Clarias gariepinus) under
closed spawning system method of artificial propagation.
5. MATERIALS AND MATEHODS
The spawning will be carried out at Joseph Sarwuan Tarka University Makurdi Hacthery unit. Wild
male and female spawners of Clarias gariepinus would be obtained in Maurdi, the Benue state
capital. Females will be selected on the basis of distended, well-developed gonads, All spawners, as a
standard procedure, will be treated prophylactically against ectoparasites, using a mixture of 0,05 mg
per litre zinc-free malachite green and 25-40 mg per litre 40% formalin for some hours prior to the
commencement of the spawning program. Holding tanks in the hatchery will be supplied with well-
aerated, fresh, aged, chlorine-free recirculated water. Fish transported to the laboratory would first
conditioned to the water used in the hatchery by gradual replacement of the transportation water over
a period of 1-2 hours. Spawning will be carried according to the following procedures;
6. MATERIALS AND MATEHODS
(Continuation)
• Prophylactic treatment of spawners against ectoparasites using the method described Leteux and
Meyer (1972).
• Transfer of male and female fish to separate tanks with clean, aerated, aged water for some hours
before commencement of artificial spawning would be done.
• First intramuscular injection of all female spawners, lateral to dorsal fin, using 200 IV of Chorulon
would be done.
• First injection of female spawners, 24 hours after first injection of a Clarias gariepinus (male or
female) would be done.
• Third injection of female spawners 30 hours after the first injection with Chorulon. Dosage consists
of PGE homogenate from one male or female donor of equivalent mass will be done.
7. MATERIALS AND MATEHODS
(Continuation)
• Second injection of male spawners with one clarias. gariepinus PGE homogenate
irrespective of the body mass of the recipient male, approximately 6 hours after
first injection.
• About 40–42 hours following the first hormone injection, female spawners will be
examined for any indication of spontaneous egg release. When this occurs,
spawners will be gently dried with towels before being stripped of their eggs by
being held firmly over the mouth with one hand while exerting pressure on the
gonads in the direction of the vaginal hole.
8. DATA ANALYSIS
Data generated would be subjected to descriptive statistics
such as frequency distribution, percentages and means to
explain the incubation period, fertilization rate and hatchability
rates of African catfish.
