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CHAPTER 25
Diagnostic
Testing
Learning
Objectives
❑Explain the phases
of diagnostic testing.
❑Describe the
complete blood
count testing.
❑Explain the serum
electrolyte, liver
function test and
lipid profile.
❑Explain the serum
glucose testing.
❑Elaborate the
examination of
stool.
❑Describe the urine
testing, urine
culture and sputum
testing.
❑Discuss the
radiologic and
endoscopic
procedures.
CHAPTER
OUTLINE
❑Phases of diagnostic
testing (pre-test, intra-
test and post-test)
❑Common investigations
and clinical implications
❑ Complete Blood Count
❑Serum Electrolytes
❑LFT
❑Lipid/Lipoprotein profile
❑Serum Glucose-AC, PC,
HbA1c
❑Monitoring Capillary
Blood Glucose
(Glucometer Random
Blood Sugar-GRBS)
❑Stool Routine Examination
(Types and collection of
specimen of faeces:
Observation)
❑Collection of urine
specimen: Observation,
urine testing-Albumin,
Acetone, pH, Specific
Gravity
❑Urine Testing- Types
❑Urine Culture, Routine,
Timed Urine Specimen
❑Sputum culture, Throat
Specimen, Vaginal
Specimen
❑Overview of Radiologic
and Endoscopic
Procedures
INTRODUCTION
The term diagnostic
consists of all the
activities in measuring
and interpreting the
growth lags and their
influences on the body.
DEFINITIONS
OF DIAGNOSTIC
TEST
•A diagnostic test is a test that helps in
locating and identifying the
abnormality, deficiency or deviation
from the normal in the different organs
or parts of the body.
•This is a medical procedure which is
performed by medically skilled
personnel to detect, identify, diagnose
and monitor the disease, its process
and then according to the condition the
course of treatment is decided.
PHASES OF DIAGNOSTIC TESTING
PRE-TEST PHASE
• The first phase in the laboratory process.
• Includes the collection of specimen, transporting and
processing of sample.
• Includes the issues regarding the handling and collection of
the specimen.
• During the pretesting phase, which is also known as the
preanalytic phase, there are chances of errors while handling
the specimen and processing the specimen.
• Therefore, the preanalytical phase should have rigorous
control measures to avoid any unnecessary hindrances or
errors.
INTRA-TEST PHASE
•The second phase is also known as the
analytic phase.
•This phase is usually considered as the
‘actual’ phase of the diagnostic testing
or laboratory testing and the ultimate
results of the diagnostic test.
POST-TEST PHASE
 The post-analytic phase is the final phase of the
laboratory process.
 This phase culminates in the production of a final
value, result, or the case of histology, a diagnostic
pathology report.
 The testing results in transmission detection,
interpretation by doctor and treatment, follow-up
and retesting to evaluate the outcome of the
condition.
COMPLETE BLOOD COUNT
This is the process of collecting or
withdrawing blood samples through
Venepuncture for routine lab
investigation to measure the various
components of blood which includes
the red blood cells, white blood cells,
hemoglobin, hematocrit and platelets.
PURPOSES OF COMPLETE BLOOD
COUNT TESTING
 To evaluate any abnormality in the
blood profile.
 To supplement the diagnosis and
treatment related to a specific
condition.
To monitor variation in the blood.
VARIOUS
COMPONENTS
OF
BLOOD
MEASURED
UNDER
COMPLETE
BLOOD
COUNT
RED BLOOD CELLS
 Function-Carry oxygen from the lungs or gills to all the body
tissues and carry carbon dioxide, a waste product of
metabolism, to the lungs, where it is excreted.
 There are lots of conditions that can affect RBC production
and a few conditions may result in significant bleeding.
 If RBCs are lost or destroyed faster than they can be replaced,
or if bone marrow production is disrupted, or if the RBCs
produced do not function normally, or do not contain enough
hemoglobin, then this condition may lead to anemia.
 If too many RBCs are produced and released, then the
condition is known as polycythemia. Polycythemia leads to
thicker blood, decreased blood flow and related problems,
such as headache, dizziness, problems with vision, and even
excessive clotting or heart attack.
NORMAL RBC VALUE
WHITE BLOOD CELLS
• Stem cells in the bone marrow are responsible
for producing white blood cells (WBCs).
• The bone marrow then stores an estimated 80–
90% of white blood cells.
• When infection or inflammatory condition
occurs, the body releases white blood cells to
help fight the infection.
TYPES OF WHITE BLOOD CELLS
NORMAL RANGE OF WBCs
LEUKOCYTOSIS
• Increased production of white blood cells in the body is known
as leukocytosis.
• There are certain conditions in which there are increased
productions of WBCs such as:
➢Conditions in which cells die such as burns, heart attack,
trauma
➢Conditions that involve an allergic response like an asthma
attack
➢Inflammatory conditions such as rheumatoid arthritis,
inflammatory bowel disease and vasculitis
➢Infections caused by bacteria, fungi and virus
➢Leukemia
LEUKOPENIA
 Certain medical conditions are there which cause decreased production of white blood
cells and it is known as leukopenia.
 It includes the following conditions:
✓ Autoimmune disorders such as systemic lupus erythematosus (SLE) and human
immunodeficiency virus (HIV)
✓ Bone marrow damage, such as from chemotherapy, radiation therapy
✓ Bone marrow disorders
✓ Lymphoma
✓ Vitamin B12 deficiencies
HEMOGLOBIN
• This is a protein in the blood cells which carries oxygen
to the different organs.
• Hemoglobin is made up of four protein molecules
(globulin chains) that are connected.
• The normal adult hemoglobin (abbreviated Hb or Hgb)
molecule contains two alpha-globulin chains and two
beta-globulin chains.
NORMAL Hb LEVEL
COMMON CAUSES OF ANEMIA
HEMATOCRIT
Proportion of red blood cells
to the fluid component or the
plasma of the blood. The
hematocrit (Ht or Hct) is
expressed as a percentage.
For example, a hematocrit of
35% means that there are 35
milliliters of red blood cells
in 100 mL of blood.
NORMAL HEMATOCRIT LEVEL
HIGH HEMATOCRIT LEVEL
The hematocrit levels are increased in the following
conditions:
• Increased risk of Dengue Shock Syndrome (DSS)
• Polycythemia vera
• Chronic obstructive pulmonary disease (COPD)
• Erythropoietin (EPO) or Erythropoietin use
• Dehydration
• Capillary leak syndrome
• Sleep apnea
• Anabolic steroids use
LOW HEMATOCRIT LEVEL
The hematocrit levels are reduced in the following
conditions:
• Anemia
• Pregnancy
• High altitude living
• Nutritional deficiency of iron, vitamin B12 or folate
• Kidney disease
• Bone marrow disease
• Leukemia, lymphoma or multiple myeloma
PLATELET
• Platelets are tiny blood cells that help the body in forming
clots to stop bleeding.
• If one of the blood vessels gets damaged, it sends out signals
to the platelets. The platelets then rush to the site of damage,
they form a plug (clot) to fix the damage.
• A normal platelet count is 150,000–450,000 platelets per
microliter of blood.
• The risk for bleeding develops if a platelet count falls below
10,000–20,000.
• When the platelet count is less than 50,000, bleeding is likely
to be more serious if there is any cut or bruises in the body.
SERUM ELECTROLYTES
Electrolytes are
involved in many
essential processes in
the body. They play a
vital role in
conducting nervous
impulses, contracting
muscles, keeping the
body hydrated and
regulating the pH
levels.
‘Electrolyte’ is the
umbrella term for
particles that carry a
positive or negative
electric charge.
In nutrition, the term
refers to essential
minerals found in
blood, sweat and
urine. When these
minerals dissolve in a
fluid, they form
electrolytes—positive
or negative ions used
in metabolic
processes.
ELECTROLYTES FOUND IN
HUMAN BODY
SERUM SODIUM
• Osmotically active anion.
• One of the most important electrolytes in the extracellular fluid.
• Responsible for maintaining the extracellular fluid volume and also for
the regulation of the membrane potential of cells.
• Hyponatremia has neurological manifestations.
• Patients may present with headache, confusion, nausea, delirium.
• Symptoms of hypernatremia include tachypnea, sleeping difficulty and
feeling restless.
• Normal range: 135–145 mmol/L
SERUM POTASSIUM
• Potassium is mainly an intracellular ion.
• The sodium-potassium adenosine triphosphatase pump has the primary responsibility for
regulating the homeostasis between sodium and potassium, which pumps out sodium in
exchange for potassium, which moves into the cells.
• Normal range: 3.6–5.5 mmol/L
• Potassium disorders are related to cardiac arrhythmias.
• Hypokalemia occurs when serum potassium levels are under 3.6 mmol/L—weakness,
fatigue, and muscle twitching present in hypokalemia.
• Hyperkalemia occurs when the serum potassium levels are above 5.5 mmol/L, which can
result in arrhythmias. Muscle cramps, muscle weakness, rhabdomyolysis, myoglobinuria are
presenting signs and symptoms in hyperkalemia.
SERUM CALCIUM
Hypercalcemia may cause abnormal heart rhythms, muscle
twitches, cramps, weakness, depression, memory loss, confusion,
coma, bone pain, osteoporosis, fractures, abdominal pain,
nausea and vomiting.
A low calcium level may cause tingling (often in the
lips, tongue, fingers, and feet), muscle aches, spasms of
the muscles in the throat (leading to difficulty
breathing), stiffening and spasms of muscles (tetany),
seizures and abnormal heart rhythms.
Calcium has a
significant
physiological role in
the body.
It is involved in skeletal
mineralization, contraction of
muscles, the transmission of
nerve impulses, blood clotting,
and secretion of hormones.
Normal range:
8.8–10.7
mg/dL
It is mostly present
in the extracellular
fluid.
SIGNS OF
HYPOCALCEMIA
SERUM MAGNESIUM
• Magnesium is an intracellular cation.
• Magnesium is mainly involved in ATP metabolism,
contraction and relaxation of muscles, proper
neurological functioning, and neurotransmitter release.
• Normal range: 1.46–2.68 mg/dL
• Hypomagnesemia: Nausea, vomiting, weakness,
decreased appetite, numbness, tingling, muscle cramps,
seizures, muscle spasticity and abnormal heart rhythms.
BICARBONATE
The acid-base status of the blood drives bicarbonate levels.
The kidneys predominantly regulate bicarbonate concentration and are responsible for
maintaining
the acid-base balance.
Kidneys reabsorb the filtered bicarbonate and also generate new bicarbonate by net acid
excretion.
Diarrhea usually results in loss of bicarbonate, thus causing an imbalance in acid-base
regulation.
Normal range: 23–30 mmol/L. It increases or decreases depending on the acid-base status.
PHOSPHORUS
•Phosphorus is an extracellular fluid cation.
•Eighty-five percent of the total body phosphorus is in
the bones and teeth in the form of hydroxyapatite.
• Phosphate is regulated simultaneously with calcium
by vitamin D3, PTH, and calcitonin.
•The kidneys are the primary avenue of phosphorus
excretion.
•Normal range: 3.4–4.5 mg/dL
CHLORIDE
Chloride is an anion found
predominantly in the
extracellular fluid. The
kidneys predominantly
regulate serum chloride
levels. Most of the chloride
is filtered by the glomerulus.
HOW TO LABELA SAMPLE?
LIVER FUNCTION TESTS (LFT)
•Liver function tests (LFTs) (also known as a liver
panel) are blood tests that measure different enzymes,
proteins, and other substances made by the liver.
•Liver function tests are most often used to diagnose
liver diseases, such as hepatitis and monitor treatment
of liver disease. These tests can show how well the
treatment is working.
ALANINE TRANSAMINASE TEST
Alanine transaminase
(ALT) is used to
metabolize protein in
the body.
If the liver is damaged
or does not function
properly, ALT can be
released into the blood.
This causes ALT levels
to increase.
RANGE OF ALT
ASPARTATE
AMINOTRANSFERASE TEST
• Aspartate aminotransferase (AST) is an enzyme found in the heart,
liver and muscles.
• AST levels aren’t as specific for liver damage as ALT.
• It’s usually measured together with ALT to check for liver
problems.
• When the liver is damaged, AST can be released into the
bloodstream.
• A high result on an AST test might indicate a problem with the liver
or muscles.
RANGE
OF AST
ALKALINE PHOSPHATASE TEST
• Alkaline phosphatase (ALP) is an enzyme found in the
bones, bile ducts, and liver.
• High levels of ALP may indicate liver inflammation,
blockage of the bile ducts, or bone disease.
ALBUMIN TEST
Albumin is
the main
protein made
by the liver.
An albumin test
measures how
well the liver is
making this
particular
protein.
A low result on
this test can
indicate that
the liver isn’t
functioning
properly.
It performs
many
important
bodily
functions.
The normal
range for
albumin is 3.5–
5.0 grams per
deciliter
(g/dL).
BILIRUBIN
TEST
• Bilirubin is a waste product from the
breakdown of red blood cells.
• It’s ordinarily processed by the liver.
• It passes through the liver before
being excreted through the stool.
• A damaged liver can’t properly
process bilirubin.
• This leads to an abnormally high
level of bilirubin in the blood.
RANGE OF BILIRUBIN
LIPID/
LIPOPROTEIN
PROFILE
•A lipid profile (or lipid panel)
is a blood test that measures
the concentrations of fats and
cholesterol in the blood, and
can be used to assess ‘good
cholesterol’ versus ‘bad
cholesterol’levels.
TYPES OF LIPIDS
SERUM
GLUCOSE –
AC, PC,
HbA1C
• Hemoglobin A1c is a measure of the
degree to which hemoglobin is
glycosylated in erythrocytes and is
expressed as a percentage of total
hemoglobin concentration.
• HbA1c levels provide an indication
of the average blood glucose
concentration during the preceding
2–3 months, including both pre-and
postprandial glycemia.
LEVEL OF HbA1C
MONITORING
CAPILLARY
BLOOD GLUCOSE
(GLUCOMETER
RANDOM BLOOD
SUGAR)
Measurement of the blood
glucose level of the patient,
who is at risk of
hyperglycemia or
hypoglycemia, using a
portable glucometer by
putting the drop of blood
on the glucometer strip
using the needle or lancet.
PURPOSES OF MONITORING
CAPILLARY BLOOD GLUCOSE
•To monitor the blood glucose level of the
patient.
•To educate the patient regarding diet
management and medication.
•To encourage the patient about compliance
with the treatment regimen.
MONITORING CAPILLARY BLOOD
GLUCOSE
ARTICLES
A clean tray containing:
• Glucometer
• Testing strips
• Sterile lancet or 26FG sterile
needle
• Dry cotton swab and alcohol swab
• Clean gloves
• Hand rub
PROCEDURE
Refer book (page no.644)
FASTING BLOOD SUGAR
• This is a test that helps in determining the amount of
glucose (sugar) in a blood sample after an overnight
fast. The fasting blood glucose test is commonly used
to detect diabetes mellitus.
• The normal range for blood glucose is 70–100 mg/dL.
• A blood sample can be taken in a lab, physician’s
office, or the hospital. The test is done in the morning,
before the person has taken breakfast.
RANDOM BLOOD SUGAR
• A random blood sugar (RBS) test is the testing of the blood sugar
level at any time or random time of the day.
• According to American Diabetes Association:
➢The RBS test is done within 1 or 2 hours of eating then the RBS
normal value should be 180 mg/dL.
➢The RBS normal range should be anywhere between 80 and 130
mg/dL prior to eating for healthy blood sugar levels in the body.
NORMAL RANGE OF
BLOOD GLUCOSE
POSTPRANDIAL BLOOD SUGAR
•The word postprandial means after a meal;
therefore, PPG concentrations refer to
postprandial plasma glucose concentrations
after eating. Postprandial plasma glucose
tests show how tolerant the body is to
glucose.
z LEVELS OF
POSTPARANDIAL BLOOD
SUGAR
STOOL ROUTINE EXAMINATION
(TYPES AND COLLECTION OF SPECIMEN
OF FAECES: OBSERVATION)
Definition:
It is a method of
obtaining stool
specimen from the
patient.
Purpose:
To check the stool
for the presence
of specific
material (blood,
ova, parasite or
bacteria like
Salmonella and
Shigella, etc.)
STOOL ROUTINE EXAMINATION
(TYPES AND COLLECTION OF SPECIMEN
OF FAECES: OBSERVATION)
Articles Procedure
• A clean specimen container
(routine examination), Sterile
specimen container (culture)
• A spatula/sterile swab stick for
stool culture
• Dry bedpan
• Clean gloves
• Disposable mask
• Tissue paper
• Laboratory requisition forms
Refer book (page no. 645)
CULTURING THE STOOL
• The stool can be cultured for disease-causing bacteria.
• A stool sample is placed in an incubator for at least
48–72 hours and any disease-causing bacteria are
identified and isolated.
• Not all bacteria in the stool cause problems; in fact,
about half of stool is bacteria, most of which live there
normally and are necessary for digestion.
CULTURING THE STOOL
Stool
culture
• For a stool culture, the lab will need a fresh or refrigerated sample of stool. The
best samples are of loose, fresh stool; well-formed stool is rarely positive for
disease-causing bacteria. Sometimes, more than one stool will be collected for a
culture.
Stool
culture
• Culture is then tested for the presence of microorganisms (gram-positive/
negative or virus or bacteria).
Stool
culture
• After identification of the organism, culture is then tested for antibiotics, that
are effective to kill the grown microorganism. Based on the results antibiotics
are added to the treatment.
TESTING THE STOOL FOR OVA AND
PARASITES
• Stool may be tested for the presence of parasites and ova (the egg stage
of a parasite) if a child has prolonged diarrhea or other intestinal
symptoms.
• The doctor will collect two or more samples of stool to successfully
identify parasites.
• If parasites or their eggs are seen when a smear of stool is examined
under the microscope, the child will be treated for a parasitic infestation.
• The hospital will provide a special collection container that contains
chemical preservatives for parasites.
COLLECTION OF URINE SPECIMEN
AND URINE TESTING
COLLECTING URINE SPECIMEN FOR CULTURE
Description
• For detecting the presence
and growth of
microorganisms in the
urine sample, a small
amount (30–60 mL) of
urine is collected.
Purposes
• To identify the antibiotic
sensitivity of the pathogen
in the urine sample.
• Culture pathogenic
microorganisms are
present in the urine.
COLLECTING URINE SPECIMEN FOR
CULTURE
Articles
• Sterile container
• Sterile needle if required in
case of catheterized client
• Soap and water
• Bedpan
• Laboratory form
Procedure
• Refer book (page no. 646)
COLLECTION OF URINE SPECIMEN FOR
ROUTINE EXAMINATION (MIDSTREAM
URINE)
Procedure
Refer book
(page no. 646)
COLLECTION OF 24 HOURS URINE
• It is a collection of the urine specimen for a period of 24 hours
without any spillage or wastage.
Description
• To detect kidney, liver and cardiac conditions.
• To measure the total proteins, creatinine and electrolyte,
hormones.
Purposes
• Clean container with lid
• Measuring jar
• Urinal and kidney tray to collect urine at each voiding
• A complete laboratory form
Articles
• Refer book (page no.
Procedure
URINE CONSTITUENTS
URINE COLOUR OBSERVATION
OBSERVED URINE TESTING
Urine test provides useful information in the
diagnosis and treatment of a variety of
disease conditions. Analysis of urine helps in
examining the appearance, odor, pH, specific
gravity and presence of albumin, sugar and
acetone.
URINALYSIS
Urinalysis is a
diagnostic, physical
and chemical,
microscopic detection
of any abnormality of
urine.
PURPOSES OF URINALYSIS
Monitoring the urine
abnormality
General evaluation of health
Monitoring the conditions like
diabetes mellitus, kidney
disease
ARTICLES FOR URINALYSIS
A clean tray containing the following articles:
Test tube holder Test tubes (4-6) on test tube stand
Spirit lamp with match box Kidney tray, paper bag
Rag pieces Dropper (2)- one for urine and
one for reagents
Litmus paper to check the
reaction of urine
Calibrated urinometer to check
the specific gravity
Wide mouth specimen jar Jar for urine
Bowl Clean gloves (1 pair)
REAGENTS
For albumin hot test—Acetic acid solution 2%
For sugar testing—Benedict’s solution
For albumin cold test—Nitric acid solution
For acetone test—Sodium nitroprusside crystal
For acetone test—Ammonium sulfate crystals
TEST FOR pH
Use litmus paper for testing pH. Dip the litmus
paper in urine and note for the change of color.
• If the red litmus turns blue, urine is alkaline.
• If the blue litmus turns red, urine is acidic.
• If there is no change in color, urine is neutral.
It is the measure of the solute concentration in
urine by using a calibrated urinometer and it helps
in assessing the ability of the renal tubules to
concentrate or dilute the glomerular filtrate. The
specific gravity ranges from 1.010 to 1.040.
CHECKING FOR URINE SPECIFIC
GRAVITY
PURPOSES FOR CHECKING URINE
SPECIFIC GRAVITY
To determine the
concentration
level of the urine.
To measure the
specific gravity
of the urine.
PROCEDURE
FOR
CHECKING
URINE
SPECIFIC
GRAVITY
Refer book (page no. 648)
TEST FOR ALBUMIN IN
URINE: HOT TEST
Description • This test (hot test) is used to find out
the presence of albumin in the urine.
Procedure • Refer book (page no. 648)
TEST FOR ALBUMIN IN URINE: COLD
METHOD
This test (cold method) is done to find out the
presence of albumin in the urine.
PROCEDURE
Refer book (page no. 649)
TEST FOR ACETONE IN URINE
Description
Testing of the urine
specimen for
identifying the
presence of
acetone.
Procedure
Refer book
(page no. 649)
BILE TESTS
Bile salt test Bile pigment test
Procedure
Refer book (page
no. 649)
Procedure
Refer book (page
no. 649)
SUGAR OR GLUCOSE TEST
•PROCEDURE
Refer book (page no. 649)
RESULT FOR GLUCOSE TEST
Reagent strips are
available for urine
testing which
yields the results
quickly. These are
available for
ketone and glucose
assessment in
urine (also called
ketostix or diastix).
Description
How to use:
Refer book (page
no. 650)
Procedure
A sputum culture is a
test that checks for
bacteria or another type
of organism that may be
causing an infection in
the lungs or the airways
leading to the lungs.
SPUTUM
CULTURE
DIFFERENT COLORS OF
SPUTUM
DIFFERENT COLORS OF
SPUTUM
COLLECTION OF SPUTUM
FOR CULTURE
Collection of coughed out sputum
for culture to identify respiratory
pathogens (acid-fast bacillus,
streptococci, pneumococci,
diphtheria bacilli).
PURPOSES OF COLLECTION OF
SPUTUM FOR CULTURE
Purposes Identify respiratory pathogens.
Look for the color of sputum like hemoptysis,
greenish color in bronchitis, rusty color in
pneumonia.
COLLECTION OF SPUTUM
FOR CULTURE
ARTICLES
• Sterile specimen container
• Tissue paper
• Sputum mug with
disinfectant
• Sterile gloves
• Disposable mask
• Requisition form
PROCEDURE
Refer book (page no. 650)
THROAT CULTURE SWAB
ARTICLES
• Sterile gloves
• Sterile test tube
• Sterile cotton
applicator or
swab stick
PROCEDURE
• Refer book
(page no. 651)
VAGINAL SWAB OR
SMEAR
ARTICLES PROCEDURE
• Two clean slides
• Cotton applicator
Refer book (page no.
651)
RADIOLOGICAL PROCEDURES
Radiology is a
branch of medicine
that uses imaging
technology to
diagnose and treat
disease.
Radiology
Diagnostic Interventional
DIAGNOSTIC RADIOLOGY
This branch helps in visualizing the structures
inside the body and helps the physician in
identifying certain medical conditions. Using these
diagnostic procedures doctors can monitor the
progression of the disease and the way how the
body responds to the treatment received.
DIAGNOSTIC RADIOLOGY
DIAGNOSTIC RADIOLOGY
DIAGNOSTIC RADIOLOGY
DIAGNOSTIC RADIOLOGY
INTERVENTIONAL
RADIOLOGY
A branch that deals with the assistance of
imaging tests such as CT scan, MRI to help in
performing the interventions related to the
treatment of medical conditions using or by
inserting small instruments or catheters, wires
into specific body parts.
EXAMPLES OF INTERVENTIONAL
RADIOLOGY
ENDOSCOPIC PROCEDURE
An endoscopy procedure involves inserting a
long, flexible tube (endoscope) down the
throat and into the esophagus to visualize the
gastrointestinal system.
ENDOSCOPY
•A procedure that is done using an endoscope to diagnose or
treat a condition.
•There are several types of endoscopy like those using natural
body openings including esophagogastroduodenoscopy (EGD)
which is often called upper endoscopy, gastroscopy and
enteroscopy.
SIGMOIDOSCOPY
An examination of the inside of the rectum and
sigmoid colon using an endoscope—a thin, lighted
flexible tube (sigmoidoscope) inserted through the
anus.
ENTEROSCOPY
A procedure that allows the
visualization of a greater portion of the
small bowel which is possible with
esophagogastroduodenoscopy (EGD).
PERCUTANEOUS ENDOSCOPIC
GASTROSTOMY
Percutaneous endoscopic
gastrostomy (PEG) is a
procedure through which a
flexible feeding tube is placed
with the assistance of an
endoscope through a small
incision in the abdominal wall
into the stomach.
UPPER ENDOSCOPY
■ Upper endoscopy allows for examination of the lining of the
upper part of the gastrointestinal (GI) tract, which includes the
esophagus, stomach and duodenum (first portion of the small
intestine).
CHAPTER
FOCUS
POINTS
• The term diagnostic consists of all the activities in measuring and
interpreting the growth lags and their influences on the body.
• Various diagnostic tests-complete blood count, serum electrolytes
test, liver function tests, blood sugar measurement, stool
examination, urine test, etc.
• Phases of diagnostic testing- pre-test phase, analytic phase and final
phase.
• There can be errors related to specimen handling, appropriateness
of proper specimen collection, and adequacy of the specimen and
its transport to the laboratory.
• Sputum culture, throat culture swab and vaginal smear are also a
part of diagnostic tests
• Radiological procedures are of two types – diagnostic radiology
that includes CT scan, MRI, X-Ray, etc. and interventional
radiology that includes angiography, cryoablation, needle biopsies,
etc.
• An endoscopic procedure involves inserting a long, flexible tube
(endoscope)down the throat and into the esophagus to visualize the
gastrointestinal system.
“Textbook of Foundation of
Nursing" by Jyoti Kathwal

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CHP-25-diagnostic-testing-wecompress.com_.pdf

  • 2. Learning Objectives ❑Explain the phases of diagnostic testing. ❑Describe the complete blood count testing. ❑Explain the serum electrolyte, liver function test and lipid profile. ❑Explain the serum glucose testing. ❑Elaborate the examination of stool. ❑Describe the urine testing, urine culture and sputum testing. ❑Discuss the radiologic and endoscopic procedures.
  • 3. CHAPTER OUTLINE ❑Phases of diagnostic testing (pre-test, intra- test and post-test) ❑Common investigations and clinical implications ❑ Complete Blood Count ❑Serum Electrolytes ❑LFT ❑Lipid/Lipoprotein profile ❑Serum Glucose-AC, PC, HbA1c ❑Monitoring Capillary Blood Glucose (Glucometer Random Blood Sugar-GRBS) ❑Stool Routine Examination (Types and collection of specimen of faeces: Observation) ❑Collection of urine specimen: Observation, urine testing-Albumin, Acetone, pH, Specific Gravity ❑Urine Testing- Types ❑Urine Culture, Routine, Timed Urine Specimen ❑Sputum culture, Throat Specimen, Vaginal Specimen ❑Overview of Radiologic and Endoscopic Procedures
  • 4. INTRODUCTION The term diagnostic consists of all the activities in measuring and interpreting the growth lags and their influences on the body.
  • 5. DEFINITIONS OF DIAGNOSTIC TEST •A diagnostic test is a test that helps in locating and identifying the abnormality, deficiency or deviation from the normal in the different organs or parts of the body. •This is a medical procedure which is performed by medically skilled personnel to detect, identify, diagnose and monitor the disease, its process and then according to the condition the course of treatment is decided.
  • 7. PRE-TEST PHASE • The first phase in the laboratory process. • Includes the collection of specimen, transporting and processing of sample. • Includes the issues regarding the handling and collection of the specimen. • During the pretesting phase, which is also known as the preanalytic phase, there are chances of errors while handling the specimen and processing the specimen. • Therefore, the preanalytical phase should have rigorous control measures to avoid any unnecessary hindrances or errors.
  • 8. INTRA-TEST PHASE •The second phase is also known as the analytic phase. •This phase is usually considered as the ‘actual’ phase of the diagnostic testing or laboratory testing and the ultimate results of the diagnostic test.
  • 9. POST-TEST PHASE  The post-analytic phase is the final phase of the laboratory process.  This phase culminates in the production of a final value, result, or the case of histology, a diagnostic pathology report.  The testing results in transmission detection, interpretation by doctor and treatment, follow-up and retesting to evaluate the outcome of the condition.
  • 10. COMPLETE BLOOD COUNT This is the process of collecting or withdrawing blood samples through Venepuncture for routine lab investigation to measure the various components of blood which includes the red blood cells, white blood cells, hemoglobin, hematocrit and platelets.
  • 11. PURPOSES OF COMPLETE BLOOD COUNT TESTING  To evaluate any abnormality in the blood profile.  To supplement the diagnosis and treatment related to a specific condition. To monitor variation in the blood.
  • 12. VARIOUS COMPONENTS OF BLOOD MEASURED UNDER COMPLETE BLOOD COUNT RED BLOOD CELLS  Function-Carry oxygen from the lungs or gills to all the body tissues and carry carbon dioxide, a waste product of metabolism, to the lungs, where it is excreted.  There are lots of conditions that can affect RBC production and a few conditions may result in significant bleeding.  If RBCs are lost or destroyed faster than they can be replaced, or if bone marrow production is disrupted, or if the RBCs produced do not function normally, or do not contain enough hemoglobin, then this condition may lead to anemia.  If too many RBCs are produced and released, then the condition is known as polycythemia. Polycythemia leads to thicker blood, decreased blood flow and related problems, such as headache, dizziness, problems with vision, and even excessive clotting or heart attack.
  • 14. WHITE BLOOD CELLS • Stem cells in the bone marrow are responsible for producing white blood cells (WBCs). • The bone marrow then stores an estimated 80– 90% of white blood cells. • When infection or inflammatory condition occurs, the body releases white blood cells to help fight the infection.
  • 15. TYPES OF WHITE BLOOD CELLS
  • 17. LEUKOCYTOSIS • Increased production of white blood cells in the body is known as leukocytosis. • There are certain conditions in which there are increased productions of WBCs such as: ➢Conditions in which cells die such as burns, heart attack, trauma ➢Conditions that involve an allergic response like an asthma attack ➢Inflammatory conditions such as rheumatoid arthritis, inflammatory bowel disease and vasculitis ➢Infections caused by bacteria, fungi and virus ➢Leukemia
  • 18. LEUKOPENIA  Certain medical conditions are there which cause decreased production of white blood cells and it is known as leukopenia.  It includes the following conditions: ✓ Autoimmune disorders such as systemic lupus erythematosus (SLE) and human immunodeficiency virus (HIV) ✓ Bone marrow damage, such as from chemotherapy, radiation therapy ✓ Bone marrow disorders ✓ Lymphoma ✓ Vitamin B12 deficiencies
  • 19. HEMOGLOBIN • This is a protein in the blood cells which carries oxygen to the different organs. • Hemoglobin is made up of four protein molecules (globulin chains) that are connected. • The normal adult hemoglobin (abbreviated Hb or Hgb) molecule contains two alpha-globulin chains and two beta-globulin chains.
  • 22. HEMATOCRIT Proportion of red blood cells to the fluid component or the plasma of the blood. The hematocrit (Ht or Hct) is expressed as a percentage. For example, a hematocrit of 35% means that there are 35 milliliters of red blood cells in 100 mL of blood.
  • 24. HIGH HEMATOCRIT LEVEL The hematocrit levels are increased in the following conditions: • Increased risk of Dengue Shock Syndrome (DSS) • Polycythemia vera • Chronic obstructive pulmonary disease (COPD) • Erythropoietin (EPO) or Erythropoietin use • Dehydration • Capillary leak syndrome • Sleep apnea • Anabolic steroids use
  • 25. LOW HEMATOCRIT LEVEL The hematocrit levels are reduced in the following conditions: • Anemia • Pregnancy • High altitude living • Nutritional deficiency of iron, vitamin B12 or folate • Kidney disease • Bone marrow disease • Leukemia, lymphoma or multiple myeloma
  • 26. PLATELET • Platelets are tiny blood cells that help the body in forming clots to stop bleeding. • If one of the blood vessels gets damaged, it sends out signals to the platelets. The platelets then rush to the site of damage, they form a plug (clot) to fix the damage. • A normal platelet count is 150,000–450,000 platelets per microliter of blood. • The risk for bleeding develops if a platelet count falls below 10,000–20,000. • When the platelet count is less than 50,000, bleeding is likely to be more serious if there is any cut or bruises in the body.
  • 27. SERUM ELECTROLYTES Electrolytes are involved in many essential processes in the body. They play a vital role in conducting nervous impulses, contracting muscles, keeping the body hydrated and regulating the pH levels. ‘Electrolyte’ is the umbrella term for particles that carry a positive or negative electric charge. In nutrition, the term refers to essential minerals found in blood, sweat and urine. When these minerals dissolve in a fluid, they form electrolytes—positive or negative ions used in metabolic processes.
  • 29. SERUM SODIUM • Osmotically active anion. • One of the most important electrolytes in the extracellular fluid. • Responsible for maintaining the extracellular fluid volume and also for the regulation of the membrane potential of cells. • Hyponatremia has neurological manifestations. • Patients may present with headache, confusion, nausea, delirium. • Symptoms of hypernatremia include tachypnea, sleeping difficulty and feeling restless. • Normal range: 135–145 mmol/L
  • 30. SERUM POTASSIUM • Potassium is mainly an intracellular ion. • The sodium-potassium adenosine triphosphatase pump has the primary responsibility for regulating the homeostasis between sodium and potassium, which pumps out sodium in exchange for potassium, which moves into the cells. • Normal range: 3.6–5.5 mmol/L • Potassium disorders are related to cardiac arrhythmias. • Hypokalemia occurs when serum potassium levels are under 3.6 mmol/L—weakness, fatigue, and muscle twitching present in hypokalemia. • Hyperkalemia occurs when the serum potassium levels are above 5.5 mmol/L, which can result in arrhythmias. Muscle cramps, muscle weakness, rhabdomyolysis, myoglobinuria are presenting signs and symptoms in hyperkalemia.
  • 31. SERUM CALCIUM Hypercalcemia may cause abnormal heart rhythms, muscle twitches, cramps, weakness, depression, memory loss, confusion, coma, bone pain, osteoporosis, fractures, abdominal pain, nausea and vomiting. A low calcium level may cause tingling (often in the lips, tongue, fingers, and feet), muscle aches, spasms of the muscles in the throat (leading to difficulty breathing), stiffening and spasms of muscles (tetany), seizures and abnormal heart rhythms. Calcium has a significant physiological role in the body. It is involved in skeletal mineralization, contraction of muscles, the transmission of nerve impulses, blood clotting, and secretion of hormones. Normal range: 8.8–10.7 mg/dL It is mostly present in the extracellular fluid.
  • 33. SERUM MAGNESIUM • Magnesium is an intracellular cation. • Magnesium is mainly involved in ATP metabolism, contraction and relaxation of muscles, proper neurological functioning, and neurotransmitter release. • Normal range: 1.46–2.68 mg/dL • Hypomagnesemia: Nausea, vomiting, weakness, decreased appetite, numbness, tingling, muscle cramps, seizures, muscle spasticity and abnormal heart rhythms.
  • 34. BICARBONATE The acid-base status of the blood drives bicarbonate levels. The kidneys predominantly regulate bicarbonate concentration and are responsible for maintaining the acid-base balance. Kidneys reabsorb the filtered bicarbonate and also generate new bicarbonate by net acid excretion. Diarrhea usually results in loss of bicarbonate, thus causing an imbalance in acid-base regulation. Normal range: 23–30 mmol/L. It increases or decreases depending on the acid-base status.
  • 35. PHOSPHORUS •Phosphorus is an extracellular fluid cation. •Eighty-five percent of the total body phosphorus is in the bones and teeth in the form of hydroxyapatite. • Phosphate is regulated simultaneously with calcium by vitamin D3, PTH, and calcitonin. •The kidneys are the primary avenue of phosphorus excretion. •Normal range: 3.4–4.5 mg/dL
  • 36. CHLORIDE Chloride is an anion found predominantly in the extracellular fluid. The kidneys predominantly regulate serum chloride levels. Most of the chloride is filtered by the glomerulus.
  • 37. HOW TO LABELA SAMPLE?
  • 38. LIVER FUNCTION TESTS (LFT) •Liver function tests (LFTs) (also known as a liver panel) are blood tests that measure different enzymes, proteins, and other substances made by the liver. •Liver function tests are most often used to diagnose liver diseases, such as hepatitis and monitor treatment of liver disease. These tests can show how well the treatment is working.
  • 39. ALANINE TRANSAMINASE TEST Alanine transaminase (ALT) is used to metabolize protein in the body. If the liver is damaged or does not function properly, ALT can be released into the blood. This causes ALT levels to increase.
  • 41. ASPARTATE AMINOTRANSFERASE TEST • Aspartate aminotransferase (AST) is an enzyme found in the heart, liver and muscles. • AST levels aren’t as specific for liver damage as ALT. • It’s usually measured together with ALT to check for liver problems. • When the liver is damaged, AST can be released into the bloodstream. • A high result on an AST test might indicate a problem with the liver or muscles.
  • 43. ALKALINE PHOSPHATASE TEST • Alkaline phosphatase (ALP) is an enzyme found in the bones, bile ducts, and liver. • High levels of ALP may indicate liver inflammation, blockage of the bile ducts, or bone disease.
  • 44. ALBUMIN TEST Albumin is the main protein made by the liver. An albumin test measures how well the liver is making this particular protein. A low result on this test can indicate that the liver isn’t functioning properly. It performs many important bodily functions. The normal range for albumin is 3.5– 5.0 grams per deciliter (g/dL).
  • 45. BILIRUBIN TEST • Bilirubin is a waste product from the breakdown of red blood cells. • It’s ordinarily processed by the liver. • It passes through the liver before being excreted through the stool. • A damaged liver can’t properly process bilirubin. • This leads to an abnormally high level of bilirubin in the blood.
  • 47. LIPID/ LIPOPROTEIN PROFILE •A lipid profile (or lipid panel) is a blood test that measures the concentrations of fats and cholesterol in the blood, and can be used to assess ‘good cholesterol’ versus ‘bad cholesterol’levels.
  • 49. SERUM GLUCOSE – AC, PC, HbA1C • Hemoglobin A1c is a measure of the degree to which hemoglobin is glycosylated in erythrocytes and is expressed as a percentage of total hemoglobin concentration. • HbA1c levels provide an indication of the average blood glucose concentration during the preceding 2–3 months, including both pre-and postprandial glycemia.
  • 51. MONITORING CAPILLARY BLOOD GLUCOSE (GLUCOMETER RANDOM BLOOD SUGAR) Measurement of the blood glucose level of the patient, who is at risk of hyperglycemia or hypoglycemia, using a portable glucometer by putting the drop of blood on the glucometer strip using the needle or lancet.
  • 52. PURPOSES OF MONITORING CAPILLARY BLOOD GLUCOSE •To monitor the blood glucose level of the patient. •To educate the patient regarding diet management and medication. •To encourage the patient about compliance with the treatment regimen.
  • 53. MONITORING CAPILLARY BLOOD GLUCOSE ARTICLES A clean tray containing: • Glucometer • Testing strips • Sterile lancet or 26FG sterile needle • Dry cotton swab and alcohol swab • Clean gloves • Hand rub PROCEDURE Refer book (page no.644)
  • 54. FASTING BLOOD SUGAR • This is a test that helps in determining the amount of glucose (sugar) in a blood sample after an overnight fast. The fasting blood glucose test is commonly used to detect diabetes mellitus. • The normal range for blood glucose is 70–100 mg/dL. • A blood sample can be taken in a lab, physician’s office, or the hospital. The test is done in the morning, before the person has taken breakfast.
  • 55. RANDOM BLOOD SUGAR • A random blood sugar (RBS) test is the testing of the blood sugar level at any time or random time of the day. • According to American Diabetes Association: ➢The RBS test is done within 1 or 2 hours of eating then the RBS normal value should be 180 mg/dL. ➢The RBS normal range should be anywhere between 80 and 130 mg/dL prior to eating for healthy blood sugar levels in the body.
  • 57. POSTPRANDIAL BLOOD SUGAR •The word postprandial means after a meal; therefore, PPG concentrations refer to postprandial plasma glucose concentrations after eating. Postprandial plasma glucose tests show how tolerant the body is to glucose.
  • 59. STOOL ROUTINE EXAMINATION (TYPES AND COLLECTION OF SPECIMEN OF FAECES: OBSERVATION) Definition: It is a method of obtaining stool specimen from the patient. Purpose: To check the stool for the presence of specific material (blood, ova, parasite or bacteria like Salmonella and Shigella, etc.)
  • 60. STOOL ROUTINE EXAMINATION (TYPES AND COLLECTION OF SPECIMEN OF FAECES: OBSERVATION) Articles Procedure • A clean specimen container (routine examination), Sterile specimen container (culture) • A spatula/sterile swab stick for stool culture • Dry bedpan • Clean gloves • Disposable mask • Tissue paper • Laboratory requisition forms Refer book (page no. 645)
  • 61. CULTURING THE STOOL • The stool can be cultured for disease-causing bacteria. • A stool sample is placed in an incubator for at least 48–72 hours and any disease-causing bacteria are identified and isolated. • Not all bacteria in the stool cause problems; in fact, about half of stool is bacteria, most of which live there normally and are necessary for digestion.
  • 62. CULTURING THE STOOL Stool culture • For a stool culture, the lab will need a fresh or refrigerated sample of stool. The best samples are of loose, fresh stool; well-formed stool is rarely positive for disease-causing bacteria. Sometimes, more than one stool will be collected for a culture. Stool culture • Culture is then tested for the presence of microorganisms (gram-positive/ negative or virus or bacteria). Stool culture • After identification of the organism, culture is then tested for antibiotics, that are effective to kill the grown microorganism. Based on the results antibiotics are added to the treatment.
  • 63. TESTING THE STOOL FOR OVA AND PARASITES • Stool may be tested for the presence of parasites and ova (the egg stage of a parasite) if a child has prolonged diarrhea or other intestinal symptoms. • The doctor will collect two or more samples of stool to successfully identify parasites. • If parasites or their eggs are seen when a smear of stool is examined under the microscope, the child will be treated for a parasitic infestation. • The hospital will provide a special collection container that contains chemical preservatives for parasites.
  • 64. COLLECTION OF URINE SPECIMEN AND URINE TESTING COLLECTING URINE SPECIMEN FOR CULTURE Description • For detecting the presence and growth of microorganisms in the urine sample, a small amount (30–60 mL) of urine is collected. Purposes • To identify the antibiotic sensitivity of the pathogen in the urine sample. • Culture pathogenic microorganisms are present in the urine.
  • 65. COLLECTING URINE SPECIMEN FOR CULTURE Articles • Sterile container • Sterile needle if required in case of catheterized client • Soap and water • Bedpan • Laboratory form Procedure • Refer book (page no. 646)
  • 66. COLLECTION OF URINE SPECIMEN FOR ROUTINE EXAMINATION (MIDSTREAM URINE) Procedure Refer book (page no. 646)
  • 67. COLLECTION OF 24 HOURS URINE • It is a collection of the urine specimen for a period of 24 hours without any spillage or wastage. Description • To detect kidney, liver and cardiac conditions. • To measure the total proteins, creatinine and electrolyte, hormones. Purposes • Clean container with lid • Measuring jar • Urinal and kidney tray to collect urine at each voiding • A complete laboratory form Articles • Refer book (page no. Procedure
  • 68.
  • 71. OBSERVED URINE TESTING Urine test provides useful information in the diagnosis and treatment of a variety of disease conditions. Analysis of urine helps in examining the appearance, odor, pH, specific gravity and presence of albumin, sugar and acetone.
  • 72. URINALYSIS Urinalysis is a diagnostic, physical and chemical, microscopic detection of any abnormality of urine.
  • 73. PURPOSES OF URINALYSIS Monitoring the urine abnormality General evaluation of health Monitoring the conditions like diabetes mellitus, kidney disease
  • 74. ARTICLES FOR URINALYSIS A clean tray containing the following articles: Test tube holder Test tubes (4-6) on test tube stand Spirit lamp with match box Kidney tray, paper bag Rag pieces Dropper (2)- one for urine and one for reagents Litmus paper to check the reaction of urine Calibrated urinometer to check the specific gravity Wide mouth specimen jar Jar for urine Bowl Clean gloves (1 pair)
  • 75. REAGENTS For albumin hot test—Acetic acid solution 2% For sugar testing—Benedict’s solution For albumin cold test—Nitric acid solution For acetone test—Sodium nitroprusside crystal For acetone test—Ammonium sulfate crystals
  • 76. TEST FOR pH Use litmus paper for testing pH. Dip the litmus paper in urine and note for the change of color. • If the red litmus turns blue, urine is alkaline. • If the blue litmus turns red, urine is acidic. • If there is no change in color, urine is neutral.
  • 77. It is the measure of the solute concentration in urine by using a calibrated urinometer and it helps in assessing the ability of the renal tubules to concentrate or dilute the glomerular filtrate. The specific gravity ranges from 1.010 to 1.040. CHECKING FOR URINE SPECIFIC GRAVITY
  • 78. PURPOSES FOR CHECKING URINE SPECIFIC GRAVITY To determine the concentration level of the urine. To measure the specific gravity of the urine.
  • 80. TEST FOR ALBUMIN IN URINE: HOT TEST Description • This test (hot test) is used to find out the presence of albumin in the urine. Procedure • Refer book (page no. 648)
  • 81.
  • 82. TEST FOR ALBUMIN IN URINE: COLD METHOD This test (cold method) is done to find out the presence of albumin in the urine. PROCEDURE Refer book (page no. 649)
  • 83. TEST FOR ACETONE IN URINE Description Testing of the urine specimen for identifying the presence of acetone. Procedure Refer book (page no. 649)
  • 84. BILE TESTS Bile salt test Bile pigment test Procedure Refer book (page no. 649) Procedure Refer book (page no. 649)
  • 85. SUGAR OR GLUCOSE TEST •PROCEDURE Refer book (page no. 649)
  • 86.
  • 88. Reagent strips are available for urine testing which yields the results quickly. These are available for ketone and glucose assessment in urine (also called ketostix or diastix). Description How to use: Refer book (page no. 650) Procedure
  • 89. A sputum culture is a test that checks for bacteria or another type of organism that may be causing an infection in the lungs or the airways leading to the lungs. SPUTUM CULTURE
  • 92. COLLECTION OF SPUTUM FOR CULTURE Collection of coughed out sputum for culture to identify respiratory pathogens (acid-fast bacillus, streptococci, pneumococci, diphtheria bacilli).
  • 93. PURPOSES OF COLLECTION OF SPUTUM FOR CULTURE Purposes Identify respiratory pathogens. Look for the color of sputum like hemoptysis, greenish color in bronchitis, rusty color in pneumonia.
  • 94. COLLECTION OF SPUTUM FOR CULTURE ARTICLES • Sterile specimen container • Tissue paper • Sputum mug with disinfectant • Sterile gloves • Disposable mask • Requisition form PROCEDURE Refer book (page no. 650)
  • 95. THROAT CULTURE SWAB ARTICLES • Sterile gloves • Sterile test tube • Sterile cotton applicator or swab stick PROCEDURE • Refer book (page no. 651)
  • 96. VAGINAL SWAB OR SMEAR ARTICLES PROCEDURE • Two clean slides • Cotton applicator Refer book (page no. 651)
  • 97. RADIOLOGICAL PROCEDURES Radiology is a branch of medicine that uses imaging technology to diagnose and treat disease. Radiology Diagnostic Interventional
  • 98. DIAGNOSTIC RADIOLOGY This branch helps in visualizing the structures inside the body and helps the physician in identifying certain medical conditions. Using these diagnostic procedures doctors can monitor the progression of the disease and the way how the body responds to the treatment received.
  • 103. INTERVENTIONAL RADIOLOGY A branch that deals with the assistance of imaging tests such as CT scan, MRI to help in performing the interventions related to the treatment of medical conditions using or by inserting small instruments or catheters, wires into specific body parts.
  • 105. ENDOSCOPIC PROCEDURE An endoscopy procedure involves inserting a long, flexible tube (endoscope) down the throat and into the esophagus to visualize the gastrointestinal system.
  • 106. ENDOSCOPY •A procedure that is done using an endoscope to diagnose or treat a condition. •There are several types of endoscopy like those using natural body openings including esophagogastroduodenoscopy (EGD) which is often called upper endoscopy, gastroscopy and enteroscopy.
  • 107. SIGMOIDOSCOPY An examination of the inside of the rectum and sigmoid colon using an endoscope—a thin, lighted flexible tube (sigmoidoscope) inserted through the anus.
  • 108. ENTEROSCOPY A procedure that allows the visualization of a greater portion of the small bowel which is possible with esophagogastroduodenoscopy (EGD).
  • 109. PERCUTANEOUS ENDOSCOPIC GASTROSTOMY Percutaneous endoscopic gastrostomy (PEG) is a procedure through which a flexible feeding tube is placed with the assistance of an endoscope through a small incision in the abdominal wall into the stomach.
  • 110. UPPER ENDOSCOPY ■ Upper endoscopy allows for examination of the lining of the upper part of the gastrointestinal (GI) tract, which includes the esophagus, stomach and duodenum (first portion of the small intestine).
  • 111. CHAPTER FOCUS POINTS • The term diagnostic consists of all the activities in measuring and interpreting the growth lags and their influences on the body. • Various diagnostic tests-complete blood count, serum electrolytes test, liver function tests, blood sugar measurement, stool examination, urine test, etc. • Phases of diagnostic testing- pre-test phase, analytic phase and final phase. • There can be errors related to specimen handling, appropriateness of proper specimen collection, and adequacy of the specimen and its transport to the laboratory. • Sputum culture, throat culture swab and vaginal smear are also a part of diagnostic tests • Radiological procedures are of two types – diagnostic radiology that includes CT scan, MRI, X-Ray, etc. and interventional radiology that includes angiography, cryoablation, needle biopsies, etc. • An endoscopic procedure involves inserting a long, flexible tube (endoscope)down the throat and into the esophagus to visualize the gastrointestinal system.
  • 112. “Textbook of Foundation of Nursing" by Jyoti Kathwal