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CENTRAL INSTITUTE OF FISHERIES
EDUCATION
FROM TECHNO INDIA UNIVERSITY, SALT LAKE , SECTOR --5
 Water quality requirements: (I)pH
(II) OXYGEN (III) FREE CARBON-
DIOXIDE (IV)MAINTAINANCE OF
ACIDITY AND ALKALINITY.
 pH = 7—8.5
 Colour= greenish-brown.
 Depth = 5ft or more.
 O2 level= 5-10 mg/l.
 Lime is added to maintain alkaline
condition
 Cow-dung is added to reduce
alkalinity exceeding 8.5 limit
DAY –1
DAY 1
 PCR & its applications-
Polymerase chain
reaction.
 Concept of Molar,
Normal , ppt(parts per
thousand), ppm(parts
per million), ppb(parts
per billion).
 Preparation of 0.01M
EDTA solution
 Preparation of N/50
sulphuric acid.
DAY 1 CONT:
 Microbiology of fishes:
 Factors affecting growth of
micro-organisms like
Temperature ,composition of
gases , water quantity (lower
water content 0.86 will
increase shelf like of fish
products).
 Sources of microbes in
packaged fish products .
 Sources of microbes in fishes
(gills , intestine, skin)
 Major causes for food
spoilage (physical , chemical,
microbes, others)
 Quality control by (I)
QUALITY ASSURANCE ; (II)
HACCP(hazard analysis
critical control point).
DAY 2.
ESTIMATION OF pH ;alkalinity ;free CO2 ; Hardness of water.
Enumeration of microbiological counts in isolated micro-
organisms from fish sample.
DAY 2 CONT:
DAY 2 CONT:
Methods of application of therapeutics for treatment of
diseased fishes.
Isolation of DNA from fish.
DAY 3
DAY 3 CONT:
FIELD TRIP TO NAIHATI :
VISITING NATIVE FISH FARMS
TOUR OF THE BREEDING TANKS
Growth hormones ; fish pituitary gland
Different sterilization techniques and management of pond water.
Discussion with fish farmers about feed quantity and quality , diseases
and their management.
Tour of the actual fish Breeding ponds.
DAY 4
DAY 5
Symptomatic diagnosis of infectious and non- infectious fish
diseases .
Maintenance of soil and water quality parameters inn intensive
aquaculture for management of fish diseases.
Gram staining.
DAY 6
Estimation of dissolved oxygen of the water
sample.
Estimation of Salinity of water sample.
Estimation of Ammonia of water sample.
Measurement of pH of soil by Barium
sulphate(1cm) ,25ml water and 10 mg
processed soil.
Post mortem analysis of fish for
detection and confirmation of
pathogen
Central institute of fisheries education training  completion pt
Central institute of fisheries education training  completion pt

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Central institute of fisheries education training completion pt

  • 1. CENTRAL INSTITUTE OF FISHERIES EDUCATION FROM TECHNO INDIA UNIVERSITY, SALT LAKE , SECTOR --5
  • 2.  Water quality requirements: (I)pH (II) OXYGEN (III) FREE CARBON- DIOXIDE (IV)MAINTAINANCE OF ACIDITY AND ALKALINITY.  pH = 7—8.5  Colour= greenish-brown.  Depth = 5ft or more.  O2 level= 5-10 mg/l.  Lime is added to maintain alkaline condition  Cow-dung is added to reduce alkalinity exceeding 8.5 limit DAY –1
  • 4.  PCR & its applications- Polymerase chain reaction.  Concept of Molar, Normal , ppt(parts per thousand), ppm(parts per million), ppb(parts per billion).  Preparation of 0.01M EDTA solution  Preparation of N/50 sulphuric acid. DAY 1 CONT:
  • 5.  Microbiology of fishes:  Factors affecting growth of micro-organisms like Temperature ,composition of gases , water quantity (lower water content 0.86 will increase shelf like of fish products).  Sources of microbes in packaged fish products .  Sources of microbes in fishes (gills , intestine, skin)  Major causes for food spoilage (physical , chemical, microbes, others)  Quality control by (I) QUALITY ASSURANCE ; (II) HACCP(hazard analysis critical control point). DAY 2.
  • 6. ESTIMATION OF pH ;alkalinity ;free CO2 ; Hardness of water. Enumeration of microbiological counts in isolated micro- organisms from fish sample. DAY 2 CONT:
  • 8. Methods of application of therapeutics for treatment of diseased fishes. Isolation of DNA from fish. DAY 3
  • 10. FIELD TRIP TO NAIHATI : VISITING NATIVE FISH FARMS TOUR OF THE BREEDING TANKS Growth hormones ; fish pituitary gland Different sterilization techniques and management of pond water. Discussion with fish farmers about feed quantity and quality , diseases and their management. Tour of the actual fish Breeding ponds. DAY 4
  • 11.
  • 12. DAY 5 Symptomatic diagnosis of infectious and non- infectious fish diseases . Maintenance of soil and water quality parameters inn intensive aquaculture for management of fish diseases. Gram staining.
  • 13.
  • 14. DAY 6 Estimation of dissolved oxygen of the water sample. Estimation of Salinity of water sample. Estimation of Ammonia of water sample. Measurement of pH of soil by Barium sulphate(1cm) ,25ml water and 10 mg processed soil. Post mortem analysis of fish for detection and confirmation of pathogen