This document outlines a 6 day training program on fisheries education. Day 1 covers topics like PCR applications, molar and ppm concentrations, and preparing EDTA and sulfuric acid solutions. Microbiology of fishes is also discussed, including factors affecting microbial growth and sources of microbes. Day 2 involves estimating water quality parameters like pH, alkalinity, CO2 and hardness. Microbial counts from fish samples will be enumerated. Day 3 includes a field trip to a fish farm to tour breeding tanks and ponds and discuss feeding and disease management with farmers. Day 4 covers symptomatic diagnosis of infectious and non-infectious fish diseases. Day 5 discusses maintaining soil and water quality for disease management and includes gram staining. Day

1. CENTRAL INSTITUTE OF FISHERIES
EDUCATION
FROM TECHNO INDIA UNIVERSITY, SALT LAKE , SECTOR --5

2.  Water quality requirements: (I)pH
(II) OXYGEN (III) FREE CARBON-
DIOXIDE (IV)MAINTAINANCE OF
ACIDITY AND ALKALINITY.
 pH = 7—8.5
 Colour= greenish-brown.
 Depth = 5ft or more.
 O2 level= 5-10 mg/l.
 Lime is added to maintain alkaline
condition
 Cow-dung is added to reduce
alkalinity exceeding 8.5 limit
DAY –1

3. DAY 1

4.  PCR & its applications-
Polymerase chain
reaction.
 Concept of Molar,
Normal , ppt(parts per
thousand), ppm(parts
per million), ppb(parts
per billion).
 Preparation of 0.01M
EDTA solution
 Preparation of N/50
sulphuric acid.
DAY 1 CONT:

5.  Microbiology of fishes:
 Factors affecting growth of
micro-organisms like
Temperature ,composition of
gases , water quantity (lower
water content 0.86 will
increase shelf like of fish
products).
 Sources of microbes in
packaged fish products .
 Sources of microbes in fishes
(gills , intestine, skin)
 Major causes for food
spoilage (physical , chemical,
microbes, others)
 Quality control by (I)
QUALITY ASSURANCE ; (II)
HACCP(hazard analysis
critical control point).
DAY 2.

6. ESTIMATION OF pH ;alkalinity ;free CO2 ; Hardness of water.
Enumeration of microbiological counts in isolated micro-
organisms from fish sample.
DAY 2 CONT:

7. DAY 2 CONT:

8. Methods of application of therapeutics for treatment of
diseased fishes.
Isolation of DNA from fish.
DAY 3

9. DAY 3 CONT:

10. FIELD TRIP TO NAIHATI :
VISITING NATIVE FISH FARMS
TOUR OF THE BREEDING TANKS
Growth hormones ; fish pituitary gland
Different sterilization techniques and management of pond water.
Discussion with fish farmers about feed quantity and quality , diseases
and their management.
Tour of the actual fish Breeding ponds.
DAY 4

12. DAY 5
Symptomatic diagnosis of infectious and non- infectious fish
diseases .
Maintenance of soil and water quality parameters inn intensive
aquaculture for management of fish diseases.
Gram staining.

14. DAY 6
Estimation of dissolved oxygen of the water
sample.
Estimation of Salinity of water sample.
Estimation of Ammonia of water sample.
Measurement of pH of soil by Barium
sulphate(1cm) ,25ml water and 10 mg
processed soil.
Post mortem analysis of fish for
detection and confirmation of
pathogen