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Energy and Metabolism
Energy and Metabolism
Energy and Cellular Work




            Eukaryotic Cell: Neuron
Energy and Metabolism
    Endergonic and Exergonic Reactions
• When do our cells
  perform exergonic
  reactions?

• When do our cell
  perform endergonic
  reactions?
Our cells perform exergonic
    reactions when …
                                                 25% 25% 25% 25%




                                                                                  2
                                                                                 nd
    1.   They make new hair




                                                                           s
                                                                        fat
                                                         in
                                            ir




                                                                               1a
                                          ha



                                                       sk



                                                                     rn
                                       w



                                                    w



                                                                   bu
                                     ne



                                                  ne



                                                                ey
    2.   They make new skin




                                      e



                                                   e
                                    ak




                                                              Th
                                                 ak
                                   m



                                                m
                                ey



                                             ey
                              Th



                                           Th
    3.   They burn fats
    4.   1 and 2
Our cells perform endergonic
     reactions when …


                                                  25% 25% 25% 25%




     1.   They make new hair
     2.   They make new skin
     3.   They burn fats




                                                                                   2
                                                                                  nd
                                                                            s
                                                                         fat
                                                          in
                                             ir
     4.   1 and 2




                                                                                1a
                                           ha



                                                        sk



                                                                      rn
                                        w



                                                     w



                                                                    bu
                                      ne



                                                   ne



                                                                 ey
                                       e



                                                    e
                                     ak




                                                               Th
                                                  ak
                                    m



                                                 m
                                 ey



                                              ey
                               Th



                                            Th
Energy and Metabolism
  Biosynthetic and Degradative Pathways
• Biosynthetic Pathway:
  Anabolism

• Degradative Pathway:
  Catabolism
Energy and Metabolism
All Living Organisms Require Energy
               Sources
The Flow of Energy or How
             Organisms Relate




                                            Consumers or Heterotrophs




Producers or Autotrophs (Photoautotrophs)
                                              Decomposers
The Flow of Energy or How
          Organisms Relate
• Photoautotrophs
  synthesize high energy
  organic molecules during
  photosynthesis
• Both photoautotrophs and
  heterotrophs use such
  organic molecules to
  obtain energy (ATP)
  through cellular
  respiration for fueling
  cellular work
Participants in Metabolic Pathways
• Substrate:


• Intermediate Product(s):



• End Product(s):

• Enzymes:

• Energy Carriers:
Photosynthetic bacteria are autotrophs because
  they have the ability to produce their own high
energy organic compounds (food). Do you agree?

                                 50%      50%




              1. Yes
              2. No               s




                                           No
                                Ye
Adenosine 5’-triphosphate (ATP)




• What part of the molecule is the most
  electronegative?
What part of the molecule is the
   most electronegative?


                              33%                33% 33%




  1.   ribose
  2.   phosphate groups



                               e




                                                            e
  3.   adenine

                             os




                                                          in
                                                 ps




                                                        en
                          rib




                                               ou




                                                      ad
                                             gr
                                         ate
                                       ph
                                     os
                                   ph
Hydrolysis of ATP into ADP




   Energy and inorganic phosphate are released.
   What for?
The ATP/ADP Cycle



From Degradative Pathways
                                              For Synthetic Pathways




                            Phosphorylation
Energy Releasing Pathways
       Fermentation




              Fermentation (alcoholic and lactic): 2 ATP’s
              produced. www.schmohz.com/beerinfo1.html
Energy Releasing Pathways
       Fermentation




  Yeast
Energy Releasing Pathways
           Anaerobic Respiration
•   Anaerobic Respiration: 2 ATP’s
    produced.
•   Commonly known diseases caused by
    anaerobic bacteria include gas
    gangrene, tetanus, and botulism.
    Nearly all dental infections are caused
    by anaerobic bacteria.
•   Anaerobic bacteria can cause an
    infection when a normal barrier (such
    as skin, gums, or intestinal wall) is
    damaged due to surgery, injury, or
    disease. Body sites that have tissue
    destruction (necrosis) or a poor blood
    supply are low in oxygen and favor the
    growth of anaerobic bacteria.
•   Anaerobic organisms perform
    anaerobic respiration
Energy Releasing Pathways
       Aerobic Respiration

• Aerobic Respiration:
  approximately 36 ATP
  molecules produced
• Aerobic organisms
  perform aerobic
  respiration
Which one of the following is the most
effective pathway for producing ATP?

                                            33% 33% 33%
  1. fermentation
  2. anaerobic respiration
  3. aerobic respiration




                                      ion




                                                                                n
                                                           n




                                                                             tio
                                                        tio
                                   tat




                                                                           ra
                                                     ira
                                 en




                                                                         pi
                                m




                                                   sp




                                                                       es
                             fer




                                                 re




                                                                     cr
                                             bic




                                                                   bi
                                                                 ro
                                           ro




                                                               ae
                                         ae
                                       an
Depending on their oxygen needs,
        organisms are
• Strictly Aerobic: require oxygen
• Facultative Aerobic

• Strictly Anaerobic: do not tolerate oxygen
• Facultative Anaerobic
Humans and plants are ___
1. strictly anaerobic                 25% 25% 25% 25%
2. facultative
   anaerobic
3. strictly aerobic
4. facultative aerobic




                                                               c



                                                                              ic
                                             bic




                                                             bi
                                            bic




                                                                            ob
                                                           ro
                                           ro



                                          ro




                                                                          er
                                                         ae
                                         ae



                                        ae




                                                                        ea
                                                        tly
                                       an



                                      an




                                                                     tiv
                                                     ric
                                    ly



                                  ve




                                                                  lta
                                 ict




                                                   st
                               ati




                                                                cu
                             str



                            ult




                                                              fa
                         fac
Steps of Aerobic Respiration
Where does aerobic respiration
          occur?
Steps of Aerobic Respiration:
               Glycolysis
• All organisms (anaerobic and
  anaerobic) break down
  glucose trough the process of
  glycolysis, which occurs in the
  cell’s cytoplasm
• Only aerobic organism process
  the products of glycolysis to
  obtain further amounts of ATP
• Glucose is actively transported
  into the cell and
  phosphorylated (step 1),
  process that turns on
  glycolysis
Steps of Aerobic Respiration:
             Glycolysis
• 2 molecules of
  pyruvate are
  produced per
  molecule of glucose
• A net of 2 ATP per
  molecule of glucose
  are produced in
  glycolysis
Steps of Aerobic Respiration:
            Krebs Cycle
• The Krebs cycle
  accomplishes two
  important functions:
  the production of
  multiple intermediate
  products, and of
  electron donors
  (NADH, FADH2)
Steps of Aerobic Respiration:
              Krebs Cycle
• The Krebs Cycle occurs in the
  mitochondrial matrix
• It is initiated when one
  molecule of pyruvate is
  transported into the
  mitochondrion by an oxygen
  dependent transport protein
• Upon entering the
  mitochondrion, pyruvate is
  turned into acetyl CoA, which
  initiates the cycle
• CO2 is produced and will
  eventually leave the
  mitochondrial matrix and
  collect in blood vessels
Where is the pyruvate coming
            from?
                                       25% 25% 25% 25%




1.   Lactic fermentation
2.   Alcoholic fermentation
3.   Aerobic respiration
4.   All of the above




                                                          ve
                                                          n
                                                       ion



                                                      ion



                                                       tio



                                                      bo
                                                    tat




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                                                 en



                                                en




                                              f th
                                              es
                                             m



                                            m



                                           cr



                                          lo
                                           er



                                         fer
                                       cf




                                        bi



                                        Al
                                     lic



                                     ro
                                    cti



                                  ho



                                  Ae
                                  La



                                co
                              Al
Steps of Aerobic Respiration:
             Krebs Cycle
• Per molecule of
  pyruvate, 1 ATP, 1
  FADH2, 3 CO2, and 4
  NADH are produced
• NADH and FADH2 will
  initiate the last step of
  aerobic respiration,
  the electron transport
  phosphorylation
  system (ETPS).
Steps of Aerobic Respiration:
                 ETPS
• ETPS consists of a series of
  proteins located in the inner
  mitochondrial membrane
• NADH and FADH2 link
  glycolysis and the Krebs cycle
  to the machinery that produces
  large amounts of ATP
• NADH and FADH2 turn on the
  ETPS. Electrons cascade
  down the chain from one
  protein to the next until they
  finally reach the molecule of
  oxygen, the final acceptor
• As electrons cascade down,
  protons are released
Steps of Aerobic Respiration:
                ETPS
• As proteins release H+ in
  the intermembrane
  space, they produce a
  gradient of H+, which
  activates ATP synthase,
  the enzyme that produces
  about 32-34 ATP
  molecules.
  Chemiosmosis refers to
  the activation of ATP
  synthase by a H+
  gradient.
Where am I?

                                         25% 25% 25% 25%




                                                              st
                                                          sm




                                                           lo
                                                           ..
                                                          .



                                                        d.
                                                      pla



                                                       e..




                                                      ely
                                                     on
                                                     th
                                                    to




                                                    et
                                                  ch
                                                  to
                                                 cy




                                                  pl
                                              t in




                                               m
                                              ito
                                            ll’s




                                           Co
                                           m
                                           ge
                                          Ce
1.   Cell’s cytoplasm




                                        he
                                      t to



                                     et
                                    ou



                                  sid
                                 Ab



                                In
2.   About to get into the
     mitochondrion
3.   Inside the mitochondrion
4.   Completely lost
Steps of Aerobic Respiration:
           ETPS
Aerobic Respiration:
What does it produce?
Plants need to burn sugars and fats
  to obtain ATP. Do you agree?
                         50%   50%




                          s




                               No
                        Ye
               1. Yes
               2. No
Other molecules other than sugars
   undergo aerobic respiration
• Proteins and fats are
  used in aerobic
  respiration for the
  production of ATP.
Other molecules other than sugars undergo
           aerobic respiration

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Cellular Respiration

  • 3. Energy and Cellular Work Eukaryotic Cell: Neuron
  • 4. Energy and Metabolism Endergonic and Exergonic Reactions • When do our cells perform exergonic reactions? • When do our cell perform endergonic reactions?
  • 5. Our cells perform exergonic reactions when … 25% 25% 25% 25% 2 nd 1. They make new hair s fat in ir 1a ha sk rn w w bu ne ne ey 2. They make new skin e e ak Th ak m m ey ey Th Th 3. They burn fats 4. 1 and 2
  • 6. Our cells perform endergonic reactions when … 25% 25% 25% 25% 1. They make new hair 2. They make new skin 3. They burn fats 2 nd s fat in ir 4. 1 and 2 1a ha sk rn w w bu ne ne ey e e ak Th ak m m ey ey Th Th
  • 7. Energy and Metabolism Biosynthetic and Degradative Pathways • Biosynthetic Pathway: Anabolism • Degradative Pathway: Catabolism
  • 8. Energy and Metabolism All Living Organisms Require Energy Sources
  • 9. The Flow of Energy or How Organisms Relate Consumers or Heterotrophs Producers or Autotrophs (Photoautotrophs) Decomposers
  • 10. The Flow of Energy or How Organisms Relate • Photoautotrophs synthesize high energy organic molecules during photosynthesis • Both photoautotrophs and heterotrophs use such organic molecules to obtain energy (ATP) through cellular respiration for fueling cellular work
  • 11. Participants in Metabolic Pathways • Substrate: • Intermediate Product(s): • End Product(s): • Enzymes: • Energy Carriers:
  • 12. Photosynthetic bacteria are autotrophs because they have the ability to produce their own high energy organic compounds (food). Do you agree? 50% 50% 1. Yes 2. No s No Ye
  • 13. Adenosine 5’-triphosphate (ATP) • What part of the molecule is the most electronegative?
  • 14. What part of the molecule is the most electronegative? 33% 33% 33% 1. ribose 2. phosphate groups e e 3. adenine os in ps en rib ou ad gr ate ph os ph
  • 15. Hydrolysis of ATP into ADP Energy and inorganic phosphate are released. What for?
  • 16. The ATP/ADP Cycle From Degradative Pathways For Synthetic Pathways Phosphorylation
  • 17. Energy Releasing Pathways Fermentation Fermentation (alcoholic and lactic): 2 ATP’s produced. www.schmohz.com/beerinfo1.html
  • 18. Energy Releasing Pathways Fermentation Yeast
  • 19. Energy Releasing Pathways Anaerobic Respiration • Anaerobic Respiration: 2 ATP’s produced. • Commonly known diseases caused by anaerobic bacteria include gas gangrene, tetanus, and botulism. Nearly all dental infections are caused by anaerobic bacteria. • Anaerobic bacteria can cause an infection when a normal barrier (such as skin, gums, or intestinal wall) is damaged due to surgery, injury, or disease. Body sites that have tissue destruction (necrosis) or a poor blood supply are low in oxygen and favor the growth of anaerobic bacteria. • Anaerobic organisms perform anaerobic respiration
  • 20. Energy Releasing Pathways Aerobic Respiration • Aerobic Respiration: approximately 36 ATP molecules produced • Aerobic organisms perform aerobic respiration
  • 21. Which one of the following is the most effective pathway for producing ATP? 33% 33% 33% 1. fermentation 2. anaerobic respiration 3. aerobic respiration ion n n tio tio tat ra ira en pi m sp es fer re cr bic bi ro ro ae ae an
  • 22. Depending on their oxygen needs, organisms are • Strictly Aerobic: require oxygen • Facultative Aerobic • Strictly Anaerobic: do not tolerate oxygen • Facultative Anaerobic
  • 23. Humans and plants are ___ 1. strictly anaerobic 25% 25% 25% 25% 2. facultative anaerobic 3. strictly aerobic 4. facultative aerobic c ic bic bi bic ob ro ro ro er ae ae ae ea tly an an tiv ric ly ve lta ict st ati cu str ult fa fac
  • 24. Steps of Aerobic Respiration
  • 25. Where does aerobic respiration occur?
  • 26. Steps of Aerobic Respiration: Glycolysis • All organisms (anaerobic and anaerobic) break down glucose trough the process of glycolysis, which occurs in the cell’s cytoplasm • Only aerobic organism process the products of glycolysis to obtain further amounts of ATP • Glucose is actively transported into the cell and phosphorylated (step 1), process that turns on glycolysis
  • 27. Steps of Aerobic Respiration: Glycolysis • 2 molecules of pyruvate are produced per molecule of glucose • A net of 2 ATP per molecule of glucose are produced in glycolysis
  • 28. Steps of Aerobic Respiration: Krebs Cycle • The Krebs cycle accomplishes two important functions: the production of multiple intermediate products, and of electron donors (NADH, FADH2)
  • 29. Steps of Aerobic Respiration: Krebs Cycle • The Krebs Cycle occurs in the mitochondrial matrix • It is initiated when one molecule of pyruvate is transported into the mitochondrion by an oxygen dependent transport protein • Upon entering the mitochondrion, pyruvate is turned into acetyl CoA, which initiates the cycle • CO2 is produced and will eventually leave the mitochondrial matrix and collect in blood vessels
  • 30. Where is the pyruvate coming from? 25% 25% 25% 25% 1. Lactic fermentation 2. Alcoholic fermentation 3. Aerobic respiration 4. All of the above ve n ion ion tio bo tat ra ea tat pi en en f th es m m cr lo er fer cf bi Al lic ro cti ho Ae La co Al
  • 31. Steps of Aerobic Respiration: Krebs Cycle • Per molecule of pyruvate, 1 ATP, 1 FADH2, 3 CO2, and 4 NADH are produced • NADH and FADH2 will initiate the last step of aerobic respiration, the electron transport phosphorylation system (ETPS).
  • 32. Steps of Aerobic Respiration: ETPS • ETPS consists of a series of proteins located in the inner mitochondrial membrane • NADH and FADH2 link glycolysis and the Krebs cycle to the machinery that produces large amounts of ATP • NADH and FADH2 turn on the ETPS. Electrons cascade down the chain from one protein to the next until they finally reach the molecule of oxygen, the final acceptor • As electrons cascade down, protons are released
  • 33. Steps of Aerobic Respiration: ETPS • As proteins release H+ in the intermembrane space, they produce a gradient of H+, which activates ATP synthase, the enzyme that produces about 32-34 ATP molecules. Chemiosmosis refers to the activation of ATP synthase by a H+ gradient.
  • 34. Where am I? 25% 25% 25% 25% st sm lo .. . d. pla e.. ely on th to et ch to cy pl t in m ito ll’s Co m ge Ce 1. Cell’s cytoplasm he t to et ou sid Ab In 2. About to get into the mitochondrion 3. Inside the mitochondrion 4. Completely lost
  • 35. Steps of Aerobic Respiration: ETPS
  • 37. Plants need to burn sugars and fats to obtain ATP. Do you agree? 50% 50% s No Ye 1. Yes 2. No
  • 38. Other molecules other than sugars undergo aerobic respiration • Proteins and fats are used in aerobic respiration for the production of ATP.
  • 39. Other molecules other than sugars undergo aerobic respiration