This study investigated whether neuronal cells show preferential growth and maturation on synthetic polymers versus decellularized nerve extracellular matrix (dECM) bioink. Several key findings were reported: 1) Neurite outgrowth of neuronal cells was greater when cultured on dECM bioink compared to matrigel or tissue culture plastic. 2) Cell viability was lower for neuronal cells grown on dECM bioink compared to matrigel or plastic, indicating dECM bioink supports longer neurites but at the expense of cell viability. 3) A blend of dECM bioink and PEG was successfully 3D printed, demonstrating a novel biomaterial containing both natural and synthetic properties that may