Can someone please help with a thorough answer for these two different questions: 1. You isolated an enveloped virus whose virions are able to hemadsorb and hemagglutinate chicken red blood cells. What other characteristics do you expect this virus to possess if is related to influenza viruses? Please explain in thorough detail and reasoning. 2. You isolated an enveloped RNA virus. Purified RNA is not capable of supporting translation in cellfree systems. Further analysis indicates that this virus replication is not inhibited by actinomycin D, a strong inhibitor of DNA-dependent RNA polymerase. Based on this information, discuss the mechanism by which this virus replicates in infected cells. Please explain in thorough detail and reasoning. Solution The infective form of the virus is called Virion. It consists of a single stranded RNA with a Protein covering.The envelopes are typically derived from the host cell proteins and phospholipids and may cotain some viral glycoproteins. These viral glycoproteins present on the envelope of the virus helps to identify and bind to the host cell membrae and may avoid the host immune system. Enveloped viruses have specific membrane fusion inducing properties which help in the entry of these virions in to the host cell. First of all influenza virions bind to the sialic acid of the cell surface of the host cell and are endocytosed.These edocytic vesicles become acidified, pH drops from 7 to around 5 ad at this pH , the hemagglutinin trimers (HA) undergoes a structural trasition i the influenza evelope of the virus. At some stage , this HA monomer splits in to two polypeptides HA1 ad HA2.Among these two, HA2 is called Fusion peptide and it is hydrophobic. This iteracts with the vesicle membrane and cause the viral evelope ad the vesicle membrae to fuse.This fusion event results in the transferring of viral genome in to the cytoplasm. Enveloped viruses are sensitive to heat, acids ad drying. These enveloped viruses are less virulent in nature. These cannot survive inside the gastrointestinal tract generally. These can induce cell mediated and antibody mediated immune response in the host. Hemadsorption is the process of adherence of red blood cells to the surface of viruses. 2.Based on the iformation given, the viruses Containing single stranded RNA can enter Host cell by the above said process that is, Fusion technique. During the process to invade the host cell, the single straded RNA udergo replication in the presence of RNA polymerase and produces invadive Fusion polypeptide, later the cell membrane of the host ad the fusion poolypeptide fuse together and the genome of the virus gets injected in to the cytoplasm of the host cell. In the cytoplasm of the Host cell, The genome of the virus becomes an integral part of the host DNA resulting in the transcription and translation . During Translation The mRNA produced consists of the genetic information of the host cell as well as the virus.This results in the e.