The document discusses several experiments and techniques used by researchers over time to study the brain and nervous system:
1) Galvani's "animal electricity" experiment in 1791 which used wires to induce muscle twitches in frogs and helped establish the connection between nerves and muscles.
2) Darwin's 1869 book which compared facial expressions and emotions in humans and animals.
3) The development of computed tomography (CT) scans in the 1970s which allowed researchers to non-invasively image the inside of the brain.
4) Nobel Prize winning work in the 1980s by Neher and Sakmann who developed a technique using micro-pipettes to study single ion channels and cellular communication.