The document outlines the procedure for collecting blood samples for culture tests to diagnose bacteremia or septicemia, detailing necessary supplies, specific collection sites, and blood volume requirements for various patient age groups. It includes a step-by-step procedure from handwashing to labeling the samples, emphasizing infection control and proper handling of the collected samples. Common microorganisms isolated from blood cultures are also listed, including s. aureus and e. coli.

1. BLOOD SAMPLE
COLLECTION FOR
CULTURE TEST.
PREPARED BY:-
MS.NISHA PRASAD.

2. DEFINITION:-
• It is a collection of blood into culture medium with the aim of
growing the microorganism to diagnose bacteremia or
septicemia.
 Bacteremia:- presence of bacteria in the bloodstream.
 Septicemia:- it is a condition in which microorganisms grow
and multiply in blood.

3. ORGANISMS COMMONLY ISOLATE FROM BLOOD
CULTURES:-
 S. aureus (Salmonella)
 E. coli
 S. pneumoniae
 Bacteriods
 clostridium

4. SITE OF COLLECTION:-
 Arterial or venous blood.
 Arterial or venous lines.

5. AMOUNT OF BLOOD:-
 For adult- 10 ml
 For infant & children- 1-5 Ml
 Neonate- 1-2 ml
 1month-2 year age- 2-3mL
 Older children – 3-5 mL

6. SUPPLIES NEEDED FOR BLOOD COLLECTION
PROCEDURE:-
 Blood culture bottles-
 Adults : aerobic bottle(green top)
anerobic bottle(orange top)
Children-
pediatric bottle (yellow top)

7.  Gloves
 Cleansing supplies:- iodine , alcohol cotton swab or gauze.
 Tourniquet.
 Patient labels.
 Laboratory test request form.

8. PROCEDURE
Wash hands.
Identify patient & explain procedure.
Observe appropriate infection control precautions for each patient;
ex:- HIV patient.
Tie tourniquet around patient’s extremity to locate vein.
Prepare draw (venipuncture) site by cleansing it.

9. Perform venipuncture using a needle and syringe.
Withdraw required amount of blood.
Withdraw needle from vein and apply pressure on
venipuncture site using a sterile gauze piece.
Inject blood into culture bottles (do not inject air into bottle).

10. Discard drawing supplies (needles) into sharp container.
Label each bottle properly.
Blood culture bottles should be kept at proper temperature
until received in laboratory.
Send bottles to directly microbiology department.