Blessing institute of peramedics mirpurkhas campus lab tech.pptx
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The document discusses hot air ovens, autoclaves, and chemicals used in a lab. It defines a hot air oven as using dry heat to sterilize equipment by effectively killing microbes. An autoclave is a pressurized heated container used for chemical reactions and sterilization through steam. It can sterilize various materials and instruments. Biochemicals and laboratory reagents are then discussed, with biochemicals exploring chemical processes in living organisms at a molecular level, and reagents being used to perform different lab tests.
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This document provides information about laboratory hazards, safety precautions, and essential equipment and facilities for biology and cytogenetics labs. It discusses three types of laboratory hazards: physical, chemical, and biological. It lists important safety precautions for handling chemicals. It also describes common equipment used in biology labs, including micropipettes, hot plates, vortex mixers, autoclaves, centrifuges, water baths, shakers, incubators, laminar flow hoods, and microscopes. Finally, it outlines specialized equipment and facilities needed for cytogenetics labs.
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Sterilization techniques .TYPES .MERTIES. AND DIMERTIES AND APPLICATION......
This document discusses sterilization techniques. It defines sterilization as any process that eliminates transmissible agents like bacteria and viruses. The main methods of sterilization discussed are physical (heat, radiation, filtration) and chemical (gaseous). Heat sterilization through moist heat like autoclaving and dry heat is the most widely used method. Radiation uses gamma rays or electrons to sterilize heat-sensitive products. Filtration removes microbes from liquids and gases. Gaseous sterilization uses chemicals like ethylene oxide or formaldehyde that react with microbes. Sterilization is important in medicine to prevent disease transmission and growth and avoid additional surgeries.
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This document provides an overview of sterilization including:
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This document provides definitions and information about sterilization including:
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- Classification of sterilization processes as terminal or non-terminal and by mechanism (physical, chemical).
- Parameters used to measure sterilization effectiveness like D-value and Z-value.
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- Parameters used to measure sterilization effectiveness like D-value and z-value
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- Definitions of sterilization, antiseptic, bacteriostatic, bactericidal, viable, disinfection, and related terms.
- Classification of sterilization processes as terminal or non-terminal and by mechanism (physical, chemical).
- Parameters used to measure sterilization effectiveness like D-value and Z-value.
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principles of sterilization, concepts, various types of sterilization methods
Sterilization refers to any process that eliminates transmissible agents like bacteria, viruses, and fungi. There are several methods of sterilization, including heat, radiation, filtration, and chemicals. Heat sterilization is the most common method and involves the use of dry heat or moist heat to kill microorganisms. Moist heat in the form of steam under pressure, as used in an autoclave, is effective at penetrating materials and achieving sterilization. Other methods include radiation like UV light or gamma rays, filtration through fine filters to physically remove microbes, and chemicals like ethylene oxide gas that sterilize without heat.
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There have many methods of sterilization.,there have also sterilization with aseptic techniques for help to education. Easy to learn about of sterilization, and how to sterile easy to learn way. So i hope you'll enjoy and learn easily and to read about this slide.
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This document summarizes various sterilization methods used in hospitals. It discusses the aims of sterilization and differentiates between sterilization, disinfection, and antisepsis. It then describes various physical sterilization methods like heat, radiation, filtration and chemical methods like alcohols, aldehydes, phenols, and halogens. Specific sterilization techniques are outlined, including autoclaving, dry heat ovens, radiation, and chemical disinfectants. Testing methods for determining the efficacy of sterilization processes are also briefly mentioned.
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This document discusses various sterilization methods used in pharmaceutical manufacturing including physical, chemical, and filtration methods. The main physical sterilization methods discussed are heat sterilization methods like dry heat and moist heat using autoclaving. Radiation and filtration methods are also covered. The document reviews the mechanisms, advantages, and applications of each method. It also discusses in-process quality controls important for ensuring sterility and quality of pharmaceutical products and materials.
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Aseptic technique, culturing and preservation by Likhith K
Aseptic technique is a method of compete elimination of microorganism, used in laboratories or clinical setting to prevent the contamination or growth of unwanted microorganism.
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Once purified, the isolated species can then be cultivated with the knowledge that only the desired microorganism is being grown.
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The document discusses various methods for sterilization and disinfection. It begins by explaining that most medical devices are heat sterilized using steam, but some materials like plastics require low-temperature sterilization. It then describes several physical methods like heat, radiation, and filtration. It also outlines some common chemical disinfecting agents like alcohol, aldehydes, phenols, halogens, and dyes. The document provides details on sterilization techniques like autoclaving and their mechanisms of action.
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The document summarizes sterilization using an autoclave. It explains that an autoclave uses high pressure and high temperature steam to kill microorganisms. It works by raising the boiling point of water when under pressure, allowing it to reach temperatures high enough to kill bacteria, viruses and fungal spores. The document outlines the main components of an autoclave, including the heating element, temperature controller and pressure sensor. It describes the working process where steam is generated and raises the temperature and pressure to 121.5°C for 15-30 minutes to effectively sterilize materials. Different types of autoclaves and sterilization methods, both dry and wet, are also summarized.
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This document discusses sterilization and disinfection methods used in pharmaceutical microbiology. It defines sterilization as killing all microorganisms, including bacterial spores, while disinfection only kills most pathogens. Physical sterilization methods like heat, radiation, and filtration are described. Heat sterilization can be achieved through dry or moist heat in an oven. Proper sterilization depends on temperature, time, type of microbe, and material. The document outlines various sterilization techniques and their applications in pharmaceutical preparations.
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This document provides information about disinfection and sterilization. It defines key terms like disinfection, antisepsis, asepsis, and discusses the difference between antiseptics and disinfectants. It describes various physical agents for sterilization including heat, radiation, and filtration. It also covers different chemical agents used for sterilization like alcohols, chlorine compounds, formaldehyde, glutaraldehyde and hydrogen peroxide. The document provides details on different sterilization techniques and the advantages and disadvantages of various physical and chemical sterilization methods.
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principles of sterilization, concepts, various types of sterilization methods
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There have many methods of sterilization.,there have also sterilization with aseptic techniques for help to education. Easy to learn about of sterilization, and how to sterile easy to learn way. So i hope you'll enjoy and learn easily and to read about this slide.
STERLIZATION AND ITS METHODS.pptx
This document summarizes various sterilization methods used in hospitals. It discusses the aims of sterilization and differentiates between sterilization, disinfection, and antisepsis. It then describes various physical sterilization methods like heat, radiation, filtration and chemical methods like alcohols, aldehydes, phenols, and halogens. Specific sterilization techniques are outlined, including autoclaving, dry heat ovens, radiation, and chemical disinfectants. Testing methods for determining the efficacy of sterilization processes are also briefly mentioned.
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This document discusses various sterilization methods used in pharmaceutical manufacturing including physical, chemical, and filtration methods. The main physical sterilization methods discussed are heat sterilization methods like dry heat and moist heat using autoclaving. Radiation and filtration methods are also covered. The document reviews the mechanisms, advantages, and applications of each method. It also discusses in-process quality controls important for ensuring sterility and quality of pharmaceutical products and materials.
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The document discusses the history of antiseptic surgery and the development of sterilization techniques. It explains that in the 19th century, surgery had high rates of infection because operating conditions were not aseptic. French scientist Louis Pasteur's work on germ theory influenced Joseph Lister to apply carbolic acid in surgery, reducing infection rates. The document then outlines various physical and chemical methods to control microbial growth through sterilization, disinfection, or inhibiting growth, including heat, radiation, filtration, and chemicals like ethylene oxide. It emphasizes that proper time and temperature application is needed to effectively eliminate microbes.
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Aseptic technique is a method of compete elimination of microorganism, used in laboratories or clinical setting to prevent the contamination or growth of unwanted microorganism.
Pure cultures are important in microbiology for the following reasons:
Once purified, the isolated species can then be cultivated with the knowledge that only the desired microorganism is being grown.
A pure culture can be correctly identified for accurate studying and testing and diagnosis in a clinical environment.
Testing/experimenting with a pure culture ensures that the same results can be achieved regardless of how many time the test is repeated.
Pure culture spontaneous mutation rate is low
Pure culture clone is 99.999% identical
To maintain pure culture for extended periods in a viable conditions, without any genetic change is referred as Preservation. The aim of preservation is to stop the cell division at a particular stage i.e. to stop microbial growth or at least lower the growth rate. Due to this toxic chemicals are not accumulated and hence viability of microorganisms is not affected.
sterilization disinfection.pdf
The document discusses various methods for sterilization and disinfection. It begins by explaining that most medical devices are heat sterilized using steam, but some materials like plastics require low-temperature sterilization. It then describes several physical methods like heat, radiation, and filtration. It also outlines some common chemical disinfecting agents like alcohol, aldehydes, phenols, halogens, and dyes. The document provides details on sterilization techniques like autoclaving and their mechanisms of action.
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Autoclave
The document summarizes sterilization using an autoclave. It explains that an autoclave uses high pressure and high temperature steam to kill microorganisms. It works by raising the boiling point of water when under pressure, allowing it to reach temperatures high enough to kill bacteria, viruses and fungal spores. The document outlines the main components of an autoclave, including the heating element, temperature controller and pressure sensor. It describes the working process where steam is generated and raises the temperature and pressure to 121.5°C for 15-30 minutes to effectively sterilize materials. Different types of autoclaves and sterilization methods, both dry and wet, are also summarized.
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This document discusses sterilization methods used in hospital operating theatres. It begins by defining sterilization and listing its objectives. It then describes various sterilization categories including physical (thermal/heat, radiation, filtration) and chemical (gaseous, liquid) methods. It focuses on steam/moist heat sterilization using autoclaves as the most common and effective method. It also discusses the roles and responsibilities of the Central Sterile Supply Department and nurses in ensuring sterilized equipment and preventing hospital-acquired infections.
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This document discusses sterilization and disinfection methods used in pharmaceutical microbiology. It defines sterilization as killing all microorganisms, including bacterial spores, while disinfection only kills most pathogens. Physical sterilization methods like heat, radiation, and filtration are described. Heat sterilization can be achieved through dry or moist heat in an oven. Proper sterilization depends on temperature, time, type of microbe, and material. The document outlines various sterilization techniques and their applications in pharmaceutical preparations.
Disinfactants
This document provides information about disinfection and sterilization. It defines key terms like disinfection, antisepsis, asepsis, and discusses the difference between antiseptics and disinfectants. It describes various physical agents for sterilization including heat, radiation, and filtration. It also covers different chemical agents used for sterilization like alcohols, chlorine compounds, formaldehyde, glutaraldehyde and hydrogen peroxide. The document provides details on different sterilization techniques and the advantages and disadvantages of various physical and chemical sterilization methods.
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Medical Quiz ( Online Quiz for API Meet 2024 ).pdf
Blessing institute of peramedics mirpurkhas campus lab tech.pptx
- 1. Blessing institute of peramedics mirpurkhas campus PRESENTED BY : ALI REHMAN MARI SUBJECT : LAB TECHNICIAN TOPIC : HOT AIR OVEN– AUTOCLAVE AND CHEMICAL DATE : 15/08/2022
- 2. Hot Air oven A hot air oven is a laboratory instrument that uses dry heat to sterilize laboratory equipment and other materials. Used to hot air oven: Hot air sterilization is one method effectively killing microbes of all kinds, especially bacteria, viruses and molds on heat-resistant materials. Contamination control during the incubation of cell cultures in a CO₂ incubator is of the greatest importance.
- 3. Autoclave
- 4. Defination and uses Of Autoclaves a strong heated container used for chemical reactions and other processes using high pressures and temperatures, e.g. steam sterilization An autoclave is used to sterilize surgical equipment, laboratory instruments, pharmaceutical items, and other materials. It can sterilize solids, liquids, hollows, and instruments of various shapes and sizes. Autoclaves vary in size, shape and functionality
- 5. Chemicals / Reagent and their uses Biochemicals Laboratory Reagents Microbiology
- 6. Biochemical and Lab Reagents Bio chemical : is the branch of science that explores the chemical processes within and related to living organisms. It is a laboratory based science that brings together biology and chemistry. Biochemistry focuses on processes happening at a molecular level. It focuses on what’s happening inside our cells. Lab reagents : A laboratory reagent we used to perform the different tests by the different reagents (e.g) for RBS we use glucose reagents we take 1000 micro reagents and 10 micro serum we swip after 10 mins and get result