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ASSIGNMENT 3
CELLULASE ENZYME
EC 3.2.1.4
SMBB 3723 – BIOCOMPUTATION AND
BIOINFORMATIC
GROUP MEMBERS
AFIQ AMSYAR BIN AHMAD (A13MB0002)
EMIRUL ISKANDAR BIN ROSLEE (A13MB0026)
SHAFIQAH HANIM BIN MUHAMMMAD SHAKIB (A13MB0151)
NUR ARIFAH BINTI HUSIN BASRI (A13MB0107)
NUR HAZIRAH BINTI MUHAMMADAN (A13MB0105)
INTRODUCTION OF
CELLULASE!
I am Cellulase’s Food
◎ Produced by fungi, bacteria and protozoa that catalyze cellulolysis
◎ Type of endoglucanase
1. History
◎ Reese et al (1950) first proposed the enzyme
mechanisms involved into degradation of cellulose
◎ Reese and Levinson made an extensive study of
the action of cellulolytic enzyme (1952)
◎ 200 genes encoding cellulase and related enzyme
have been cloned
◎ Characterized genetically and biochemically
”
2. FUNCTION AND REACTION
◎ Use in hydrolyzing cellulose to become simple
sugar (glucose)
◎ Breakdown β-1,4-glycosidic bond (hydrogen bond)
3. INDUSTRIAL APPLICATION
◉ In food
◎ Processing in coffee
◎ Perform hydrolysis of cellulose during drying the beans
◉ Textile and laundry detergent
◉ Pulp and paper industry
◉ Pharmaceutical application
◉ Use in fermentation of biomass into biofuel
◉ Beverage
◎ Wine
PSYCHROPHILE (1TVN)
◎ Sources: Antartic
◎ Produce by Pseudoaltromonas sp.
◎ Optimum temperature: 25°C
◎ pH: 6-7
◎ Glycosyl hydrolase Family 5
◎ 492 amino acid
THERMOPHILE (4ENG)
◎ Source: BIOTEC culture collection
◎ Produced by Syncephalastrum racemosum
◎ Optimum temperature: 70°C
◎ pH: 5-6
◎ Glycosyl hydrolase family 45
◎ 32 amino acid
MESOPHILE (1GA2)
◎ Source: Genbank Accession No 434793
◎ Produce by Clostridium cellulovorans
◎ Optimum temperature: 40°C
◎ pH: 5-8
◎ Glycosyl hydrolase Family 48
◎ 703 amino acid
SUPERIMPOSE
THE END

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Bioinfo

  • 1. ASSIGNMENT 3 CELLULASE ENZYME EC 3.2.1.4 SMBB 3723 – BIOCOMPUTATION AND BIOINFORMATIC
  • 2. GROUP MEMBERS AFIQ AMSYAR BIN AHMAD (A13MB0002) EMIRUL ISKANDAR BIN ROSLEE (A13MB0026) SHAFIQAH HANIM BIN MUHAMMMAD SHAKIB (A13MB0151) NUR ARIFAH BINTI HUSIN BASRI (A13MB0107) NUR HAZIRAH BINTI MUHAMMADAN (A13MB0105)
  • 3. INTRODUCTION OF CELLULASE! I am Cellulase’s Food ◎ Produced by fungi, bacteria and protozoa that catalyze cellulolysis ◎ Type of endoglucanase
  • 4. 1. History ◎ Reese et al (1950) first proposed the enzyme mechanisms involved into degradation of cellulose ◎ Reese and Levinson made an extensive study of the action of cellulolytic enzyme (1952) ◎ 200 genes encoding cellulase and related enzyme have been cloned ◎ Characterized genetically and biochemically
  • 5. ” 2. FUNCTION AND REACTION ◎ Use in hydrolyzing cellulose to become simple sugar (glucose) ◎ Breakdown β-1,4-glycosidic bond (hydrogen bond)
  • 6. 3. INDUSTRIAL APPLICATION ◉ In food ◎ Processing in coffee ◎ Perform hydrolysis of cellulose during drying the beans ◉ Textile and laundry detergent ◉ Pulp and paper industry ◉ Pharmaceutical application ◉ Use in fermentation of biomass into biofuel ◉ Beverage ◎ Wine
  • 7. PSYCHROPHILE (1TVN) ◎ Sources: Antartic ◎ Produce by Pseudoaltromonas sp. ◎ Optimum temperature: 25°C ◎ pH: 6-7 ◎ Glycosyl hydrolase Family 5 ◎ 492 amino acid
  • 8. THERMOPHILE (4ENG) ◎ Source: BIOTEC culture collection ◎ Produced by Syncephalastrum racemosum ◎ Optimum temperature: 70°C ◎ pH: 5-6 ◎ Glycosyl hydrolase family 45 ◎ 32 amino acid
  • 9. MESOPHILE (1GA2) ◎ Source: Genbank Accession No 434793 ◎ Produce by Clostridium cellulovorans ◎ Optimum temperature: 40°C ◎ pH: 5-8 ◎ Glycosyl hydrolase Family 48 ◎ 703 amino acid