Bio Printing Handbook and Bio Inks for 3D Printing organs

1. Presented by –
Sushant B. Jadhav (18PBT206)
Under the guidance of –
Prof. Vandana B. Patravale
1

2.  The aim of 3D bioprinting is to construct, in vitro,
tissues, organs and other biological systems that
mimic their native counterparts
 The materials used consist of natural and synthetic
polymers, living cells, drugs, growth factors
 3D bioprinting is an interdisciplinary field,
requiring knowledge from developmental biology,
stem cell science, chemistry, computer science, and
materials science
2

3. Deciding
application
Selection of
cells
Selection of
biomaterial
Formulating
bioink
Deciding
technique of
bioprinting
Fabrication
process
Computer
program
Bioprinting
Validation
and
application
3

4. 2. Bioprinting
technology
Droplet-
based
Inkjet
bioprinting
Acoustic-
droplet-
ejection
bioprinting
Micro-
valve
bioprinting
Extrusion-
based
Laser-induced
forward
transfer
Stereolitho-
graphy
4

5. Design Factors and the capabilities
of Bioprinting
Design Considerations in
Bioprinting
Bioprinting Fabrication Procedures
Methods to enhance Bioprinting
5

6. 6

7. 7
 Shape and Resolution
 Material Heterogeneity
1. Heterogeneous Cells
2. Heterogeneous Biomaterials
3. Graduated Growth Factors
 Cellular-Material Remolding Dynamism

8. 8

9. 9
Bioprintin
g
Fabricatio
n
Procedure
s
Direct
Printin
g
In-
Process
Crosslin
king
Hybrid
Printin
g
Indirec
t
Printin
g
Post-
Process
Crosslin
king

10. 10

11.  Integrating Native and Engineered Tissue
 Coordinating Cell-Material Interaction
 Smart Biomaterials
1. Self-Assembly Materials
2. Stimulus Responsive Hydrogel
 Power Simulation
11

12. 12
4. Bio-
inks
Hydrogels
Photocrosslin
kable inks
Thermo-
responsive
inks
Microcarriers
Cell
aggregates
Cell pellets
Tissue
spheroids
Tissue
strands
Decellularize
d matrix
components

13. 13
Natural and naturally-
derived polymers Synthetic polymers
Gelatin Pluronics
Collagen poly(N-
isopropylacrylamide)
(PNIPAAM)
Elastin
Fibroin
Fibrin
Alginate
Hyaluronic acid (HA)

14.  Functionalization for improving mechanical
integrity
 Functionalization for improving printability
 Functionalization for enhancing biocompatibility
and bioactivity
14

15. 15
Figure :Schematic summary of various methods of functionalization

16. Tissue engineering and regenerative
medicine
Transplantation and clinical
applications
Drug testing and high-throughput
screening
Cancer research 16

17. 7.1 Pluronic F127 hydrogel characterization and biofabrication in
cellularized constructs for tissue engineering applications
 Emilia Gioffredi et. al. developed a method for printing cellularised
scaffolds from thermosensitive hydrogels.
17
Figure : A, B) FEG-SEM micrographs of a four-layered hydrogel
scaffold (needle Ø 200 μm, fiber spacing 600 μm); C, D) transversal
sections of the fabricated fibers.

18. 7.2 Tailoring mechanical properties of decellularized
extracellular matrix bioink by vitamin B2-induced photo-
crosslinking
 Jinah Jang et. al. developed two-step process to solidified bio-
ink.
18
Figure : Schematic illustration of a two-step crosslinking mechanism that
applies concurrent crosslinking of vitamin-B2-induced covalent crosslinking
and thermal crosslinking

19.  Bioprinting is one of the promising technology
expanding its horizon
 Integration of bioprinting techniques to enhance the
printing process is a good strategy
 Bio-inks are core of bio-printing
 Different bio-inks with different bio-printing qualities
thus we need to choose one according to our need
 There is no idle bio-ink for printing thus each bio-ink
can be functionalized for increasing its property
according to need of technique
 Synchronization of bioprinting and bioinks can
definitely aid researchers to develop new applications
of it 19

20.  Gudapati H, Dey M, Ozbolat I., 2016, “A comprehensive review on
droplet-based bioprinting: Past, present and future”, Volume 102,
Pages 20-42.
 Ji Eun Kim, Soo Hyun Kim, Youngmee Jung, 2016, “Current
status of three-dimensional printing inks for soft tissue
regeneration”, Volume 13, Pages 636-646.
 Jia Min Lee and Wai Yee Yeong, 2016, “Design and Printing
Strategies in 3D Bioprinting of Cell-Hydrogels: A Review”, Adv.
Healthcare Mater., Volume 5, Pages 2556-2565.
 Ilze Donderwinkel, Jan C. M. van Hest and Neil R. Cameron,
2017, “Bio-inks for 3D bioprinting: recent advances and future
prospects”, Polym. Chem., Volume 8, Pages 4451-4471.
 Ozbolat IT, Yu Y., 2013, “Bioprinting toward organ fabrication:
challenges and future trends”, IEEE Trans Biomed Eng, Volume
60, Pages 691-699.
20

21. 21
THANK
YOU