Bacterail culture methods by prajith .v
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Bacterail culture
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Bacterail culture methods by prajith .v
- 1. BACTERAIL CULTURE METHODS Prajith.V PharmD @Sri Ramakrishna Institute of Paramedical Sciences Prajithkanna@gmail.com
- 2. BACTERAIL NUTRITONS AND THE DESIGN OF CULTURE MEDIA . Based on bacterial Metabolism* . Culture pH . Culture Oxidation-reduction Potential . Gaseous Requirements . Oxygen, Carbon dioxide and other gases
- 3. OXYGEN CONCENTRATIONS Aerobs Anaerobs (do not require Oxygen) Obligate Anaerobs (die in the presence of Oxygne) Facultative anaerobs (E.coli) Microaerophilic Bacterial
- 4. PURPOSE OF CULTURING Isolation Properties of bacteria To create antigens for laboratory use Typing with Bacteriophages and Bacteriocins Susceptibility To test for Antibiotic sensitivity Estimate viable counts Maintain stock cultures
- 5. METHODS OF ISOLATIONS OF PURE CULTURE WITH SURFACE PLATING ENRICHMENT MEDIUM SELECTIVE MEDIUM INDICATOR MEDIUM
- 7. TEMPERATURE AND BACTERIAL GROWTH
- 8. THE REQUIREMENTS FOR GROWTH PHYSICAL REQUIREMENTS
- 9. METHODS TO ISOLATE THE BACTERIA Streak Culture Stroke Stab Pour plate Liquid culture Special Methods for anaerobic cultures
- 10. STREAK CULTURE
- 11. How do you count bacteria ? Comparing Bacteria Counts of Water Samples. Put a drop of water of well water on a sterile plate of TGY or other agar. Spread it uniformly over the surface of the agar with any non-absorbent sterile tool. ... Incubate the plate at 30C or room temperature (r.t.). Examine the plate as often as you like. ... Count the colonies.
- 12. What is a cell counter? A counting chamber,(also known as hemocytometer), is a microscope slide that is especially designed to enable cell counting. The slide has a sink in its middle; the area of the sink is marked with a grid. A drop of a cell culture is placed in the sink
- 13. What is a serial Dilution in Microbiology? We can dilute once, then dilute this dilution, only to dilute this dilution, and so on until we get to the appropriate concentration of cells. This is called a serial dilution. A serial dilution is a series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration.
- 14. How do you calculate viable cell count? Take the average cell count from each of the sets of 16 corner squares. Multiply by 10,000 (104). Multiply by 5 to correct for the 1:5 dilution from the Trypan Blue addition.