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IB4.3.2H Images



  Enzymes and temperature
•Describe how temperature affects enzyme action
•H: explain changes in enzyme activity with pH in
terms of changes to the shape of the active site
explain why enzymes operate best in their
optimum conditions

Keywords- Denature, enzyme,
optimum, active site, lock and key
model,
                                          © Oxford University Press 2011
IB4.3.2H Images


 Why plants do not grow much in the
 winter (in the UK)?
 Ideas may include
 less light and so less photosynthesis
  and lower temperatures.


This lesson will investigate how temperature
can affect how well cells function. This
ultimately depends on the enzymes that
catalyse chemical reactions inside cells
                                         © Oxford University Press 2011
IB4.3.2H Images




What is the name of chemicals that control
chemical reactions in cells ________
and what they are made of _________


   Egg white is protein – high temperatures have a
   significant effect on the structure of most
   proteins


                                            © Oxford University Press 2011
IB4.3.2H Images




Review homework questions.

Note: if you had more difficulty with the
questions. There is an opportunity to work
with them during the next group activity.




                                      © Oxford University Press 2011
IB4.3.2H Images




Animation IB4.2.4 Enzymes (part two) to
investigate what happens to the reaction
when the temperature is increased.
complete Activity AB4.3.1 Enzymes and
temperature




                                      © Oxford University Press 2011
IB4.3.2H Images




‘Icefish’ such as Antarctic
cod are active at 2°C
because their enzymes
work best at low
temperatures. Most
organisms would be dead
or very sluggish at 2°C.




                              © Oxford University Press 2011
IB4.3.2H Images



                  How an enzyme
                  reaction can be
                  stopped by a
                  rise in
                  temperature.
                  A change is
                  shape of the
                  active site, the
                  molecule no
                  longer fits!




                           © Oxford University Press 2011
IB4.3.2H Images




       Bacteria living in hot
       springs have enzymes that
       withstand high
       temperatures.




                                   © Oxford University Press 2011
IB4.3.2H Images




The optimum temperature of an enzyme. The reaction rate
is plotted at different temperatures – all other conditions are
kept constant
                                                        © Oxford University Press 2011
IB4.3.2H Images




                  The enzyme
                  used in this
                  reaction has an
                  optimum pH of
                  7.0




                            © Oxford University Press 2011
IB4.3.2H Images




:Look  back through your work the worksheet
enzymes and temperature. Here we used an
animation to predict what would happen to the
collisions, speed and oxygen output when the
temperature changed.




                                          © Oxford University Press 2011

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B4 03 enzymes and temperature

  • 1. IB4.3.2H Images Enzymes and temperature •Describe how temperature affects enzyme action •H: explain changes in enzyme activity with pH in terms of changes to the shape of the active site explain why enzymes operate best in their optimum conditions Keywords- Denature, enzyme, optimum, active site, lock and key model, © Oxford University Press 2011
  • 2. IB4.3.2H Images Why plants do not grow much in the winter (in the UK)? Ideas may include less light and so less photosynthesis and lower temperatures. This lesson will investigate how temperature can affect how well cells function. This ultimately depends on the enzymes that catalyse chemical reactions inside cells © Oxford University Press 2011
  • 3. IB4.3.2H Images What is the name of chemicals that control chemical reactions in cells ________ and what they are made of _________ Egg white is protein – high temperatures have a significant effect on the structure of most proteins © Oxford University Press 2011
  • 4. IB4.3.2H Images Review homework questions. Note: if you had more difficulty with the questions. There is an opportunity to work with them during the next group activity. © Oxford University Press 2011
  • 5. IB4.3.2H Images Animation IB4.2.4 Enzymes (part two) to investigate what happens to the reaction when the temperature is increased. complete Activity AB4.3.1 Enzymes and temperature © Oxford University Press 2011
  • 6. IB4.3.2H Images ‘Icefish’ such as Antarctic cod are active at 2°C because their enzymes work best at low temperatures. Most organisms would be dead or very sluggish at 2°C. © Oxford University Press 2011
  • 7. IB4.3.2H Images How an enzyme reaction can be stopped by a rise in temperature. A change is shape of the active site, the molecule no longer fits! © Oxford University Press 2011
  • 8. IB4.3.2H Images Bacteria living in hot springs have enzymes that withstand high temperatures. © Oxford University Press 2011
  • 9. IB4.3.2H Images The optimum temperature of an enzyme. The reaction rate is plotted at different temperatures – all other conditions are kept constant © Oxford University Press 2011
  • 10. IB4.3.2H Images The enzyme used in this reaction has an optimum pH of 7.0 © Oxford University Press 2011
  • 11. IB4.3.2H Images :Look back through your work the worksheet enzymes and temperature. Here we used an animation to predict what would happen to the collisions, speed and oxygen output when the temperature changed. © Oxford University Press 2011

Editor's Notes

  1. ‘ Ice fish’ such as Antarctic cod are active at 2°C because their enzymes work best at low temperatures. Most organisms would be dead or very sluggish at 2°C. Rick Price/Corbis
  2. How an enzyme reaction can be stopped by a rise in temperature.
  3. Bacteria living in hot springs have enzymes that withstand high temperatures. Simon Fraser/Science Photo Library
  4. The optimum temperature of an enzyme. The reaction rate is plotted at different temperatures – all other conditions are kept constant.
  5. The enzyme used in this reaction has an optimum pH of 7.0.