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ISSN 1807-5274
                                                             Rev. Clín. Pesq. Odontol., Curitiba, v. 4, n. 2, p. 101-105, maio/ago. 2008
                                                                                        ©Revista de Clínica e Pesquisa Odontológica




            APPLICATION OF STEREOLITHOGRAPHY IN
           MANDIBULAR RECONSTRUCTION FOLLOWING
            RESECTION OF AMELOBLASTOMA: case report

        Aplicação da estereolitografia na reconstrução mandibular após ressecção de
                               ameloblastoma: relato de caso

            Camila Lopes Cardoso1, Etiene de Andrade Munhoz2, Eduardo Dias Ribeiro3,
             José Bonifácio de Souza Neto4, Eduardo Sant’Ana5, Osny Ferreira Júnior6
1
    DDS, MSc student, Department of Stomatology, Bauru Dental School, University of São Paulo, Bauru, SP - Brazil.
2
    DDS, MSc, PhD student, Department of Stomatology, Bauru Dental School, University of São Paulo, Bauru, SP - Brazil.
3
    DDS, Oral and Maxillofacial Surgeon, Bauru Dental School, University of São Paulo, Bauru, SP - Brazil, e-mail:
    eduardodonto@yahoo.com.br
4
    DDS,Oral and Maxillofacial Surgeon, Bauru, SP - Brazil.
5
    DDS, PhD, Oral and Maxillofacial surgeon, Department of Stomatology Bauru Dental School, University of São Paulo, Bauru,
    SP - Brazil.
6
    DDS, PhD, Professor, Department of Stomatology, Bauru Dental School, University of São Paulo, Bauru, SP - Brazil.




            Abstract
            A 32-year-old female patient complaining about swelling in the right side of the mandible was
            referred to our Institution. Clinical exam showed erythema and crepitation during palpation in the
            region. CT showed a multilocular lesion involving the angle, ramus and part of the right body of
            the mandible. Incisional biopsy confirmed the diagnosis of ameloblastoma. The surgical treatment
            performed was partial resection with safety margin and reconstruction with titanium plaques. The
            reported case describes, in details, the application of stereolithography for presurgical planning of
            mandibular reconstruction following resection of ameloblastoma.

            Keywords: Stereolithography; Mandibular reconstruction; Rapid prototyping; Ameloblastoma.


            Resumo

            Paciente de 32 anos, sexo feminino, com queixa de aumento de volume no lado direito da
            face, foi indicada para nossa instituição. Ao exame clínico, apresentou tumoração no lado
            direito do corpo mandibular, eritema e crepitação à palpação da região. TC revelou lesão


                                 Rev Clín Pesq Odontol. 2008 maio/ago;4(2):101-105
102     Cardoso CL, Munhoz EA de, Ribeiro ED, Souza Neto JB de, Sant’Ana E, Ferreira Júnior O.

         multilocular comprometendo o ângulo, ramo e parte do corpo mandibular direito. O exame
         microscópico da lesão apresentou diagnóstico de ameloblastoma. O tratamento cirúrgico foi
         ressecção parcial da mandíbula com margens de segurança e reconstrução com placa de
         titânio. Descreve-se o planejamento cirúrgico da reconstrução mandibular, utilizando a
         estereolitografia no planejamento.

        Palavras-chave:        Estereolitografia; Reconstrução mandibular; Prototipagem rápida;
                               Ameloblastoma



CASE REPORT                                             Design). With the aid of a CAM (Computer Aided
                                                        Manufacturing) system, and specific equipment,
          A 32-year-old white female patient            the physical model was built. The equipment
complaining of swelling on the right side of the        consists of a tank filled with acrylic resin, epoxy
face was referred to our institution. Extraoral         or vinyl, constituted by monomers, photo-
examination revealed a slight asymmetry on the          initiators and additives. The model is built on a
right side of the mandible. The patient reported        mobile platform situated immediately below the
suppuration in the region of mandibular molars.         surface of this tank.
Erythema and crepitation during palpation in the                   The computer sends to the platform the
region were found on clinical examination. The          first layer of the virtual model to be polymerized.
CT showed 4 cm in diameter multilocular                 When this layer is hit by the laser, photo-initiators
radiolucent lesion, compromising angle, ramus           trigger a localized reaction that fosters the
and part of the right body of the mandible,             formation of a polymeric chain between the
delimited by moderately irregular margins.              monomer’s molecules dispersed in the resin and
          Aspiratory punch and incisional biopsy were   solidification takes place.
carried out for microscopic examination. The                       An elevator dives the platform in the
microscopic diagnosis was compatible with               liquid polymer bath and the laser beam creates the
ameloblastoma, following a follicular microscopic       second layer of solid polymer on top of the first
pattern.                                                layer. The numerical control of the machine
          A partial resection of the mandible in the    positions this platform on the surface of the resin
area affected by the lesion was planned with adequate   and galvanometric mirrors directs the laser beam
safety margins. A prototype or model of the area to     to the portion of resin corresponding to this first
be reconstructed was chosen, through the                layer. The process is repeated successively until
stereolithography method (rapid prototyping type).      the prototype is complete. Once ready the solid
          In order to obtain models through this        model is removed from the liquid polymer bath
technique the helicoidal computerized tomograph         and washed. The supports are removed and the
should obtain reformatted axial cuts of about 1,0       model is placed in an ultraviolet radiation oven to
mm in thickness to reproduce precisely all the          undergo a complete polymerization.
details captured. After the image is captured,                     With the model in hands, precise
following the prototype manufacturer’s                  measurements were obtained, which were
requirements, specific programs generate a virtual      transferred to the patient with extreme precision.
three-dimensional model which is utilized in the        Therefore, it provided a tactile perception of the
real model reconstruction process.                      lesion. Modeling and adaptation of the
          In the case at issue, the program used        reconstruction plaque, in the 2.7mm system, were
was the 3D CAD system (Computer Aided                   performed from the model (Figure 1).




                            Rev Clín Pesq Odontol. 2008 maio/ago;4(2):101-105
Application of stereolithography in mandibular reconstruction following resection of ameloblastoma    103




FIGURE 1 - Plaque modelling and adaptation



         Resection of the lesion with safety margins
was performed and the previously molded                    FIGURE 3 - Final computerized tomography
reconstruction plaque was installed (Figure 2 e 3).

                                                           DISCUSSION

                                                                     Ameloblastoma is classified as a benign
                                                           odontogenic tumor, although its behavior is similar
                                                           to that of true neoplasia, for being locally invasive,
                                                           aggressive and leads to a radical treatment planning,
                                                           since the conservative ones has recurrence reports
                                                           (1, 2). The prognosis of the solid pattern is obscure;
                                                           thus, the treatment of choice is generally radical.
                                                           Due to the relapsing potential, in the face of great
                                                           resections, the patients seek a treatment with
                                                           functional and aesthetic results. Hence, the
                                                           planning requires new technologies in the
                                                           reconstruction techniques.
                                                                     Technological advances in image
                                                           collection, through more and more developed
                                                           plans such as helicoidal computerized tomography,
FIGURE 2 - Installation of premolded plaque                have allowed the creation of a prototype or model
                                                           construction technology, in a reduced time, known
                                                           as Rapid Prototyping (RP). It is a process of organs
                                                           and objects reproduction by scanning the area to
                                                           be prototyped, usually through a computerized
                                                           tomography or magnetic resonance exams.




                            Rev Clín Pesq Odontol. 2008 maio/ago;4(2):101-105
104      Cardoso CL, Munhoz EA de, Ribeiro ED, Souza Neto JB de, Sant’Ana E, Ferreira Júnior O.

          Rapid prototyping systems emerged in 1987,       REFERENCES
through industrial engineering dominating the
automobile, consumption goods, aeronautic and naval        1. Neville BW, Damm DD, Bouquot JE. Oral
industries, pioneering the stereolithography process          and maxillofacial patholog y. 2 nd ed.
(SLA). Nowadays, it is well utilized in the health            Philadelphia: WB Saunders; 1995.
area, besides surpassing the other techniques by its
greater precision and finishing of the model. It is        2. Regezi JA, Sciuba JJ. Patologia Bucal:
widely utilized in architecture, engineering and              correlações clinicopatológicas. Rio de
industrial design. In medicine, it is applied mainly in       Janeiro: Guanabara Koogan; 2000.
orthopedics, neurosurgery and oncology. In dentistry       3. Bouyssie JF, Bouyssie S, Sarrack P, Duran D.
its application is useful in oral and maxillofacial           Stereolithographic models derived from x-ray
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implants, and is less used in orthodontic planning,           Surg Radiol Anat. 1997;19(3):193-9.
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when necessary, and found out that most of the                orthognatic surgery. Int J Adult Orthodon
samples thought it useful in all indications (5). Others      Orthognath Surg. 1997;12(3):226-32.
authors, analyzing 40 patients with craniofacial
deformities, who underwent reconstruction surgeries        5. Erickson DM, Chance D, Schmitt S, Mathis
utilizing stereolithographic models, concluded that           J. An opinion survey of reported benefits
stereolithography is well indicated for planning and          from the use of stereolithographic models.
treatment; however, there are some limiting factors           J Oral Maxillofac Surg. 1999;57(9):1040-3.
such as manufacturing time and financial costs (6).        6. D’Urso PS, Atkinson RL, Laningan MW,
          As for anatomy precision, Barker carried            Earwaker WJ, Br uce IJ, Holmes A.
out a study measuring dimensions of objects and               Stereolithographic (SL) biomodelling in
comparing with their replicas, and obtained, as a             craniofacial surger y. Br J Plast Surg.
result 12% dimensional accuracy of the anatomical             1998;51(7):522-30.
replicas (7).
          In cases of tumor treatment it has to be         7. Barker TM, Earwaker WJS, Lisle DA.
taken into account the functional reestablishment             Accuracy of stereolithographic models of
and the aesthetic result of facial contour; therefore,        human anatomy. Australas Radiol
great precision is needed (2, 3, 7, 8).                       1994;38(2):106-11.
          The model can be used as a treatment and
didactic material for the parents and patient              8. Stoker NG, Mankovich NJ, Valentino D.
themselves. Its role in the follow up of lesions has          Stereolithographic models for surgical
been described for being excellent preoperative               planning: preliminary report. J Oral
anatomical references and serving as a parameter              Maxillofac Surg. 1992;50(5):466-71.
for posterior comparison (3).                              9. Epply BL, Sadaore AM. Computer
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surgical planning of facial intra-osseous tumors,             truction of cranial and facial deformities. J
providing precision in the resection with all osseous         Craniofac Surg. 1998;9(6):548-56.
limits, facilitating also the adaptation of metallic
prostheses, helping to preserve facial aesthetic and       10. Peckitt NS. Stereolithography and the
reducing significantly the surgery and anesthesia time.        manufacture of customized implants in
          The model also enables the surgeon to                facial reconstruction a flapless surgical
determine the required length of the plate as well as          technique. Br J Oral Maxillofac Surg.
length and number of screws before surgery (8-13).             1998;36(6):481.




                              Rev Clín Pesq Odontol. 2008 maio/ago;4(2):101-105
Application of stereolithography in mandibular reconstruction following resection of ameloblastoma   105

11. James WJ, Slabbekoorn MA, Edgin WA,
    Hardin, CK. Correction of congenital
    malar hypoplasia using stereolithography
    for presurgical planning. J Oral Maxillofac
    Surg. 1998;56(4):512-7.
12. Ker nan BT, Wimsalt JA Use of a
    stereolithography model for accurate,
    preoperative       adaptation        of     a
    reconstruction plate. J Oral Maxillofac Surg.
    2000;58(3):349-51.
13. Curcio R, Perin GL, Chilvarquer I, Borri
    ML, Ajzen S. Use of models in surgical
    predictability of oral rehabilitations. Acta
    Cir Bras. 2007;22(5):387-95.

                          Recebido: 20/03/2007
                            Received: 03/20/2007

                             Aceito: 25/04/2007
                            Accepted: 04/25/2007




                            Rev Clín Pesq Odontol. 2008 maio/ago;4(2):101-105

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Application of stereolithography in mandibular reconstruction following resection of ameloblastoma case report

  • 1. ISSN 1807-5274 Rev. Clín. Pesq. Odontol., Curitiba, v. 4, n. 2, p. 101-105, maio/ago. 2008 ©Revista de Clínica e Pesquisa Odontológica APPLICATION OF STEREOLITHOGRAPHY IN MANDIBULAR RECONSTRUCTION FOLLOWING RESECTION OF AMELOBLASTOMA: case report Aplicação da estereolitografia na reconstrução mandibular após ressecção de ameloblastoma: relato de caso Camila Lopes Cardoso1, Etiene de Andrade Munhoz2, Eduardo Dias Ribeiro3, José Bonifácio de Souza Neto4, Eduardo Sant’Ana5, Osny Ferreira Júnior6 1 DDS, MSc student, Department of Stomatology, Bauru Dental School, University of São Paulo, Bauru, SP - Brazil. 2 DDS, MSc, PhD student, Department of Stomatology, Bauru Dental School, University of São Paulo, Bauru, SP - Brazil. 3 DDS, Oral and Maxillofacial Surgeon, Bauru Dental School, University of São Paulo, Bauru, SP - Brazil, e-mail: eduardodonto@yahoo.com.br 4 DDS,Oral and Maxillofacial Surgeon, Bauru, SP - Brazil. 5 DDS, PhD, Oral and Maxillofacial surgeon, Department of Stomatology Bauru Dental School, University of São Paulo, Bauru, SP - Brazil. 6 DDS, PhD, Professor, Department of Stomatology, Bauru Dental School, University of São Paulo, Bauru, SP - Brazil. Abstract A 32-year-old female patient complaining about swelling in the right side of the mandible was referred to our Institution. Clinical exam showed erythema and crepitation during palpation in the region. CT showed a multilocular lesion involving the angle, ramus and part of the right body of the mandible. Incisional biopsy confirmed the diagnosis of ameloblastoma. The surgical treatment performed was partial resection with safety margin and reconstruction with titanium plaques. The reported case describes, in details, the application of stereolithography for presurgical planning of mandibular reconstruction following resection of ameloblastoma. Keywords: Stereolithography; Mandibular reconstruction; Rapid prototyping; Ameloblastoma. Resumo Paciente de 32 anos, sexo feminino, com queixa de aumento de volume no lado direito da face, foi indicada para nossa instituição. Ao exame clínico, apresentou tumoração no lado direito do corpo mandibular, eritema e crepitação à palpação da região. TC revelou lesão Rev Clín Pesq Odontol. 2008 maio/ago;4(2):101-105
  • 2. 102 Cardoso CL, Munhoz EA de, Ribeiro ED, Souza Neto JB de, Sant’Ana E, Ferreira Júnior O. multilocular comprometendo o ângulo, ramo e parte do corpo mandibular direito. O exame microscópico da lesão apresentou diagnóstico de ameloblastoma. O tratamento cirúrgico foi ressecção parcial da mandíbula com margens de segurança e reconstrução com placa de titânio. Descreve-se o planejamento cirúrgico da reconstrução mandibular, utilizando a estereolitografia no planejamento. Palavras-chave: Estereolitografia; Reconstrução mandibular; Prototipagem rápida; Ameloblastoma CASE REPORT Design). With the aid of a CAM (Computer Aided Manufacturing) system, and specific equipment, A 32-year-old white female patient the physical model was built. The equipment complaining of swelling on the right side of the consists of a tank filled with acrylic resin, epoxy face was referred to our institution. Extraoral or vinyl, constituted by monomers, photo- examination revealed a slight asymmetry on the initiators and additives. The model is built on a right side of the mandible. The patient reported mobile platform situated immediately below the suppuration in the region of mandibular molars. surface of this tank. Erythema and crepitation during palpation in the The computer sends to the platform the region were found on clinical examination. The first layer of the virtual model to be polymerized. CT showed 4 cm in diameter multilocular When this layer is hit by the laser, photo-initiators radiolucent lesion, compromising angle, ramus trigger a localized reaction that fosters the and part of the right body of the mandible, formation of a polymeric chain between the delimited by moderately irregular margins. monomer’s molecules dispersed in the resin and Aspiratory punch and incisional biopsy were solidification takes place. carried out for microscopic examination. The An elevator dives the platform in the microscopic diagnosis was compatible with liquid polymer bath and the laser beam creates the ameloblastoma, following a follicular microscopic second layer of solid polymer on top of the first pattern. layer. The numerical control of the machine A partial resection of the mandible in the positions this platform on the surface of the resin area affected by the lesion was planned with adequate and galvanometric mirrors directs the laser beam safety margins. A prototype or model of the area to to the portion of resin corresponding to this first be reconstructed was chosen, through the layer. The process is repeated successively until stereolithography method (rapid prototyping type). the prototype is complete. Once ready the solid In order to obtain models through this model is removed from the liquid polymer bath technique the helicoidal computerized tomograph and washed. The supports are removed and the should obtain reformatted axial cuts of about 1,0 model is placed in an ultraviolet radiation oven to mm in thickness to reproduce precisely all the undergo a complete polymerization. details captured. After the image is captured, With the model in hands, precise following the prototype manufacturer’s measurements were obtained, which were requirements, specific programs generate a virtual transferred to the patient with extreme precision. three-dimensional model which is utilized in the Therefore, it provided a tactile perception of the real model reconstruction process. lesion. Modeling and adaptation of the In the case at issue, the program used reconstruction plaque, in the 2.7mm system, were was the 3D CAD system (Computer Aided performed from the model (Figure 1). Rev Clín Pesq Odontol. 2008 maio/ago;4(2):101-105
  • 3. Application of stereolithography in mandibular reconstruction following resection of ameloblastoma 103 FIGURE 1 - Plaque modelling and adaptation Resection of the lesion with safety margins was performed and the previously molded FIGURE 3 - Final computerized tomography reconstruction plaque was installed (Figure 2 e 3). DISCUSSION Ameloblastoma is classified as a benign odontogenic tumor, although its behavior is similar to that of true neoplasia, for being locally invasive, aggressive and leads to a radical treatment planning, since the conservative ones has recurrence reports (1, 2). The prognosis of the solid pattern is obscure; thus, the treatment of choice is generally radical. Due to the relapsing potential, in the face of great resections, the patients seek a treatment with functional and aesthetic results. Hence, the planning requires new technologies in the reconstruction techniques. Technological advances in image collection, through more and more developed plans such as helicoidal computerized tomography, FIGURE 2 - Installation of premolded plaque have allowed the creation of a prototype or model construction technology, in a reduced time, known as Rapid Prototyping (RP). It is a process of organs and objects reproduction by scanning the area to be prototyped, usually through a computerized tomography or magnetic resonance exams. Rev Clín Pesq Odontol. 2008 maio/ago;4(2):101-105
  • 4. 104 Cardoso CL, Munhoz EA de, Ribeiro ED, Souza Neto JB de, Sant’Ana E, Ferreira Júnior O. Rapid prototyping systems emerged in 1987, REFERENCES through industrial engineering dominating the automobile, consumption goods, aeronautic and naval 1. Neville BW, Damm DD, Bouquot JE. Oral industries, pioneering the stereolithography process and maxillofacial patholog y. 2 nd ed. (SLA). Nowadays, it is well utilized in the health Philadelphia: WB Saunders; 1995. area, besides surpassing the other techniques by its greater precision and finishing of the model. It is 2. Regezi JA, Sciuba JJ. Patologia Bucal: widely utilized in architecture, engineering and correlações clinicopatológicas. Rio de industrial design. In medicine, it is applied mainly in Janeiro: Guanabara Koogan; 2000. orthopedics, neurosurgery and oncology. In dentistry 3. Bouyssie JF, Bouyssie S, Sarrack P, Duran D. its application is useful in oral and maxillofacial Stereolithographic models derived from x-ray surgery, in orthognatic surgeries (model surgery) and computed tomography: reproduction accuracy. implants, and is less used in orthodontic planning, Surg Radiol Anat. 1997;19(3):193-9. analyzing the real positioning of the teeth (3, 4). Erickson investigated the opinion of surgeons on the 4. Hibi H, Sawaki Y, Ueda M. Three- use of SLA in planning, diagnosis and treatment, dimensional model simulation in when necessary, and found out that most of the orthognatic surgery. Int J Adult Orthodon samples thought it useful in all indications (5). Others Orthognath Surg. 1997;12(3):226-32. authors, analyzing 40 patients with craniofacial deformities, who underwent reconstruction surgeries 5. Erickson DM, Chance D, Schmitt S, Mathis utilizing stereolithographic models, concluded that J. An opinion survey of reported benefits stereolithography is well indicated for planning and from the use of stereolithographic models. treatment; however, there are some limiting factors J Oral Maxillofac Surg. 1999;57(9):1040-3. such as manufacturing time and financial costs (6). 6. D’Urso PS, Atkinson RL, Laningan MW, As for anatomy precision, Barker carried Earwaker WJ, Br uce IJ, Holmes A. out a study measuring dimensions of objects and Stereolithographic (SL) biomodelling in comparing with their replicas, and obtained, as a craniofacial surger y. Br J Plast Surg. result 12% dimensional accuracy of the anatomical 1998;51(7):522-30. replicas (7). In cases of tumor treatment it has to be 7. Barker TM, Earwaker WJS, Lisle DA. taken into account the functional reestablishment Accuracy of stereolithographic models of and the aesthetic result of facial contour; therefore, human anatomy. Australas Radiol great precision is needed (2, 3, 7, 8). 1994;38(2):106-11. The model can be used as a treatment and didactic material for the parents and patient 8. Stoker NG, Mankovich NJ, Valentino D. themselves. Its role in the follow up of lesions has Stereolithographic models for surgical been described for being excellent preoperative planning: preliminary report. J Oral anatomical references and serving as a parameter Maxillofac Surg. 1992;50(5):466-71. for posterior comparison (3). 9. Epply BL, Sadaore AM. Computer Hence, stereolithography is a useful way for generated patient models for recons- surgical planning of facial intra-osseous tumors, truction of cranial and facial deformities. J providing precision in the resection with all osseous Craniofac Surg. 1998;9(6):548-56. limits, facilitating also the adaptation of metallic prostheses, helping to preserve facial aesthetic and 10. Peckitt NS. Stereolithography and the reducing significantly the surgery and anesthesia time. manufacture of customized implants in The model also enables the surgeon to facial reconstruction a flapless surgical determine the required length of the plate as well as technique. Br J Oral Maxillofac Surg. length and number of screws before surgery (8-13). 1998;36(6):481. Rev Clín Pesq Odontol. 2008 maio/ago;4(2):101-105
  • 5. Application of stereolithography in mandibular reconstruction following resection of ameloblastoma 105 11. James WJ, Slabbekoorn MA, Edgin WA, Hardin, CK. Correction of congenital malar hypoplasia using stereolithography for presurgical planning. J Oral Maxillofac Surg. 1998;56(4):512-7. 12. Ker nan BT, Wimsalt JA Use of a stereolithography model for accurate, preoperative adaptation of a reconstruction plate. J Oral Maxillofac Surg. 2000;58(3):349-51. 13. Curcio R, Perin GL, Chilvarquer I, Borri ML, Ajzen S. Use of models in surgical predictability of oral rehabilitations. Acta Cir Bras. 2007;22(5):387-95. Recebido: 20/03/2007 Received: 03/20/2007 Aceito: 25/04/2007 Accepted: 04/25/2007 Rev Clín Pesq Odontol. 2008 maio/ago;4(2):101-105