2. APLICATION OF PROBIOTIC AND MOLASES FOR
IMPROVING OF REARING MEDIA ON CRABLET
MASS PRODUCTION OF SWIMMING CRAB
(Portunus pelagicus, Linn)
Sugeng Raharjo
Nana S.S. Udi Putra
Eddy Nurcahyono
Makulau Sultan
3. Introduction
• CBAD Takalar has been producing crablet of
Portunus pelagicus since 2005
• Crablet production was unstable
• Attacking of pathogen bacteria (Vibrio sp) caused
a production loss
• Environmant was become an issue global-food
safety and human health
• Preventive treatment: probiotic added for
reduction pathogen bacteria but its has a short
life time, so need the substrute for feeding
• Probiotic plays as a biological balance for bacteria
pathogen like Vibrio sp.
4. Objective
• To know the effectiveness of probiotic
bacteria and molases toward increasing the
survival rate of swimming crab larvae
5. Materials and methods
• Probiotic : Commercial product ”Develop TM”
• Molases: Sugarcan factory in Takalar – South
Sulawesi Indonesia
6. Materials and Methods
• Conical fiber pond : 250 L
• Stocking density : 60 ind/mL
• Rearing period : 15 days
• Diet :
zoeae-1 to zoeae-4 : Branchionus plicatilis
(density 10-15 ind/ml) and fatty acid Ω3– HUFA
(100 ppm) and multivitamin (100 ppm)
zoeae-3 until finish : Artemia salina (4 times per
day)
7. Materials and Methods
• Treatments :
1. Probiotic : 10 ppm
2. Probiotic with molases : 10 ppm of probiotic
and 100 ppm of molases
• added every 3 day after water exchange
8. Data Collecting and Analysis
• Growth stage of larvae
• Survival rate
• Population of bacteria
• Water quality
10. 100 Figure 1.
Population of Growth stage (%) Population of Growth stage (%)
90 20%
Growth stage development of
80
70
60% swimming crab larvae (A)
60 90% Treatment of Probiotic and
Population of growth stage (%) 50 molases and (B) Treatment of
40 A probiotic only.
30
20
10
0
100 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Rearing of the day
20% 10%
90 Addition of molases into
Zoeae-1 Zoeae-2 Zoeae-3 Zoeae-4 Megalops Crablet
80
70
water rearing media of
60 swimming crab was
50 accelerated growth stage.
40 B Its mean that larvaes have a
30
high growth performance
20
10 than using probiotic only.
0
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Rearing period
Zoeae-1 Zoeae-2 Zoeae-3 Zoeae-4 Megalops Crablet
11. 0.0025
A Figure 2.
Probiotic
0.0020 Growth of swimming carb
Probiotic and
Molases
larvae in rearing period (A)
0.0015 body weight and (B) lenght of
Weight (g)
carapace
0.0010
0.0005
0.0000 Body weight and Lenght of
1.80 1 3 5 7 9 11 13 15
carapace of probiotic and
1.60 B
Probiotic molases treatment were more
1.40
lenght and weight than
Lenght of carapace (mm)
Probiotic and
1.20 Molases
treatment probiotic only but
1.00
not significantly different
0.80
(P>0,05).
0.60
Have a tending that probiotic
0.40
and molases treatment was
0.20
0.00
higher performance growth
1 3 5 7 9 11 13 15
Rearing period
12. •Addition of molases into water rearing media of swimming crab tends
to reduce the growth stage, as larvae growth was optimal.
•In the final rearing period showed lenght of carapace and weight body of
treatment probiotic and molases were more lenght and weight than
treatment of probiotic only but not significantly different (P>0,05).
But unsignificant different between both treatment might be caused by
molases concentration and C-N ratio of feed were not optimal yet
13. 100
Survival rate of probiotic and
Probiotic molases treatment were higher
80
Probiotic and Molases
survival rate than treatment
SURVIVAL RATE
probiotic only but not
Survival rate (%)
60 significantly different (P>0,05)
for Z2 and Crablet stage.
40 Thereby Probiotic and molases
played an important role in
20
increasing SR.
0
Z1 Z2 Z3 Z4 M C
Growth stage
Figure 3. Survival rate of each stage on rearing period of swimming crab larvae
14. Table 2. Population density of bacteria total and Vibrio sp. on each growth stage (cfu/ml).
Treatments Parameters Growth Stage
Zoeae-1 Zoeae-2 Zoeae-3 Zoeae-4 Megalop
POPULATION OF BACTERIA
Probiotic Bacteria Total 2,4 X 103 2,0 X 104 3,2 X 104 1,1 X 103 6,0 X 104
Vibrio sp 2,0 X 102 2,0 X 103 1,2 X 103 2,1 X 102 4,2 X 103
Probiotic and Bacteria Total 2,0 X 104 3,4 X 104 4,6 X 104 4,2 X 106 4,2 X 106
Molases Vibrio sp 3,2 X 103 3 X 102 3,2 X 102 2,2 X 102 2 X 102
Treatment of probiotic and molases was higher population density of
bacteria total than treatment of probiotic only. Its indicated that molases plays an
important role in providing nutrition for probiotic.
In the both treatments showed that the presence of population of
probiotic bacteria will be able to inhibit the population of bactera pathogen.
Aplication probiotic in the water media will have benefit more if probiotic could
growth and developed well, furthermore nutrition should be provided. So in this
research addition of molases as substrate and nutrition also important.
15. Table 3. Water quality performance in both treatments probiotic and probiotic with molases of
rearing of swimming crab larvae (Portunus pelagicus,linn)
Parameters Treatment
Probiotic Probiotic dan Molases
WATER QUALITY
Dissolved Oxygen (mg/l) 4,8 – 5,6 4,6 – 6,2
pH 7,8 – 8,6 8,1 – 8,4
Temperature (0C) 27 – 31 27 – 31
Salinity (ppt) 33 – 35 33 -35
Ammonia (mg/l) Not detected Not detected
Nitrit (mg/l) Not detected Not detected
16. Conclusion & Recommendation
Application of the probiotic bacteria with molases in water media of swimming
crab larvae (Portunus pelagicus,linn) tends to increase the survival rate, body weight, lenght
of carapace, and able to accelerate the growth of zoeae stage, in addition also controlled
the population growth of Vibrio sp (102 cfu/ml). Furthermore using probiotic with molases
was effective in improving of rearing water media on mass producing crablet of swimming
crab.
For the further it should be conducted the detail study in using of concentration
molases, probiotic density and other combination substrate for getting the high survival
rate and growth such as C-N ratio.
17. Further Information
CENTER FOR BRACKISHWATER AQUACULTURE DEVELOPMENT
TAKALAR
ADDRESS :
BONTOLOE VILLAGE SOUTH GALESONG –TAKALAR 92245
SOUTH SULAWESI – INDONESIA
TEL : 62-411-320730
FAX: 62-418-2327666
