Apoptosis, also known as programmed cell death, is a process where cells self-destruct to maintain homeostasis. It allows the body to eliminate cells that are damaged, unneeded, or potentially harmful. Apoptosis is an important process for health as it helps rid the body of these cells and shapes organs during development. In cancer cells, apoptosis is inhibited, allowing uncontrolled growth and spread of the cells. Understanding apoptosis and how it is blocked in cancer is important for developing new treatments and preventing cancer progression.
Autophagy the housekeeper in every cellfathi neana
Autophagy is a catabolic process involving the degradation of a cell’s own components through the lysosomal machinery. It is a tightly regulated process that plays a normal part in cell growth, development, and homeostasis, helping to maintain a balance between the synthesis, degradation, and subsequent recycling of cellular products.
It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Autophagy is an evolutionarily conserved mechanism of cellular self-digestion in which proteins and organelles are degraded through delivery to lysosomes. Defects in this process are implicated in numerous human diseases including cancer.
Cell death, particularly apoptosis, is probably one of the
most widely-studied subjects among cell biologists.
Understanding apoptosis in disease conditions is very
important as it not only gives insights into the pathogenesis
of a disease but may also leaves clues on how
the disease can be treated. In cancer, there is a loss of
balance between cell division and cell death and cells
that should have died did not receive the signals to do
so. The problem can arise in any one step along the way
of apoptosis.Apoptosis is an ordered and orchestrated cellular process that occurs in physiological and pathological conditions.
It is also one of the most studied topics among cell biologists. An understanding of the underlying mechanism of
apoptosis is important as it plays a pivotal role in the pathogenesis of many diseases. In some, the problem is due
to too much apoptosis, such as in the case of degenerative diseases while in others, too little apoptosis is the
culprit. Cancer is one of the scenarios where too little apoptosis occurs, resulting in malignant cells that will not
die. The mechanism of apoptosis is complex and involves many pathways. Defects can occur at any point along
these pathways, leading to malignant transformation of the affected cells, tumour metastasis and resistance to
anticancer drugs. Despite being the cause of problem, apoptosis plays an important role in the treatment of
cancer as it is a popular target of many treatment strategies. The abundance of literature suggests that targeting
apoptosis in cancer is feasible. However, many troubling questions arise with the use of new drugs or treatment
strategies that are designed to enhance apoptosis and critical tests must be passed before they can be used safely
in human subjects.. It is used,
in contrast to necrosis, to describe the situation in
which a cell actively pursues a course toward death
upon receiving certain stimule
Cancer is describes as the disease that results due to cellular changes and these changes cause the uncontrolled growth and division of cells. A cell receives instructions to die so that the body can replace it with a newer cell that functions better. Cancerous cells lack the components that instruct them to stop dividing and to die. Chemotherapeutic drugs CDs are the most widespread worldwide modality used in cancer treatment, and other autoimmune diseases. However, their non selective mechanism of action affects both cancerous and non cancerous cells, that resulting in well documented side effects. Nurses are at risk of suffering side effects. Little negligence or mistake may lead to adverse unpleasant effects for patients, staff and environment. Miss. Madhu Rajput | Mr. Raghavendran M "Chemotherapeutic Drugs" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd43941.pdf Paper URL: https://www.ijtsrd.com/medicine/nursing/43941/chemotherapeutic-drugs/miss-madhu-rajput
Austin Journal of Molecular and Cellular Biology research is an international scholarly peer reviewed Open Access journal, aims to promote the research in Journal of Molecular and Cellular Biology, which is closely related to molecular biology, developmental biology, immunology, biochemistry, genetics, biological sciences, cancer research, cellular microbiology etc.,
Austin Journal of Molecular and Cellular Biology is a comprehensive Open Access peer reviewed scientific Journal that covers multidisciplinary fields. We provide limitless access towards accessing our literature hub with colossal range of articles. The journal aims to publish high quality varied article types such as Research, Review, Short Communications, Case Reports, Perspectives (Editorials), Clinical Images.
Austin Journal of Molecular and Cellular Biology supports the scientific modernization and enrichment in Journal of Molecular and Cellular Biology research community by magnifying access to peer reviewed scientific literary works. Austin also brings universally peer reviewed member journals under one roof thereby promoting knowledge sharing, collaborative and promotion of multidisciplinary science.
Natural Compounds as Adjuncts for Treating Colon Cancer through Apoptotic Pat...ijtsrd
Cancer is one of the deadliest diseases which is caused when abnormal cells divide uncontrollably and destroy body tissue. Colorectal cancer CRC , also known as colon cancer, is one of the most common type of cancers in humans is closely linked to the global cancer – related mortalities worldwide. The ongoing clinical treatment for colorectal cancer or colon cancer largely engages surgery and chemotherapy. But as there are many side effects and due to emergence of drug resistance, it has become very necessary to find novel and more effectual drugs for colon cancer treatment. Chemo prevention, a novel method for controlling cancer encompasses the use of specific natural products or synthetic chemical agents to reverse, suppress or prevent premalignancy before the development of invasive cancer. Many studies have signified that various natural products have efficacious anti CRC effects and may be used as substitutional chemotherapy agents for CRC therapy. Various natural compounds have been shown to be promising on the basis of their anti cancerous effects and low toxicity. In this review we summarise the natural compounds having anti CRC effects from distinct sources. This review suggest that they provide a novel opportunity for treatment of colon cancer. Sarita Negi | Nikita Kaushik | Runjhun Mathur | Abhimanyu Kumar Jha "Natural Compounds as Adjuncts for Treating Colon Cancer through Apoptotic Pathway" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-4 , June 2021, URL: https://www.ijtsrd.compapers/ijtsrd42387.pdf Paper URL: https://www.ijtsrd.compharmacy/biotechnology-/42387/natural-compounds-as-adjuncts-for-treating-colon-cancer-through-apoptotic-pathway/sarita-negi
Autophagy the housekeeper in every cellfathi neana
Autophagy is a catabolic process involving the degradation of a cell’s own components through the lysosomal machinery. It is a tightly regulated process that plays a normal part in cell growth, development, and homeostasis, helping to maintain a balance between the synthesis, degradation, and subsequent recycling of cellular products.
It is a major mechanism by which a starving cell reallocates nutrients from unnecessary processes to more-essential processes. Autophagy is an evolutionarily conserved mechanism of cellular self-digestion in which proteins and organelles are degraded through delivery to lysosomes. Defects in this process are implicated in numerous human diseases including cancer.
Cell death, particularly apoptosis, is probably one of the
most widely-studied subjects among cell biologists.
Understanding apoptosis in disease conditions is very
important as it not only gives insights into the pathogenesis
of a disease but may also leaves clues on how
the disease can be treated. In cancer, there is a loss of
balance between cell division and cell death and cells
that should have died did not receive the signals to do
so. The problem can arise in any one step along the way
of apoptosis.Apoptosis is an ordered and orchestrated cellular process that occurs in physiological and pathological conditions.
It is also one of the most studied topics among cell biologists. An understanding of the underlying mechanism of
apoptosis is important as it plays a pivotal role in the pathogenesis of many diseases. In some, the problem is due
to too much apoptosis, such as in the case of degenerative diseases while in others, too little apoptosis is the
culprit. Cancer is one of the scenarios where too little apoptosis occurs, resulting in malignant cells that will not
die. The mechanism of apoptosis is complex and involves many pathways. Defects can occur at any point along
these pathways, leading to malignant transformation of the affected cells, tumour metastasis and resistance to
anticancer drugs. Despite being the cause of problem, apoptosis plays an important role in the treatment of
cancer as it is a popular target of many treatment strategies. The abundance of literature suggests that targeting
apoptosis in cancer is feasible. However, many troubling questions arise with the use of new drugs or treatment
strategies that are designed to enhance apoptosis and critical tests must be passed before they can be used safely
in human subjects.. It is used,
in contrast to necrosis, to describe the situation in
which a cell actively pursues a course toward death
upon receiving certain stimule
Cancer is describes as the disease that results due to cellular changes and these changes cause the uncontrolled growth and division of cells. A cell receives instructions to die so that the body can replace it with a newer cell that functions better. Cancerous cells lack the components that instruct them to stop dividing and to die. Chemotherapeutic drugs CDs are the most widespread worldwide modality used in cancer treatment, and other autoimmune diseases. However, their non selective mechanism of action affects both cancerous and non cancerous cells, that resulting in well documented side effects. Nurses are at risk of suffering side effects. Little negligence or mistake may lead to adverse unpleasant effects for patients, staff and environment. Miss. Madhu Rajput | Mr. Raghavendran M "Chemotherapeutic Drugs" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd43941.pdf Paper URL: https://www.ijtsrd.com/medicine/nursing/43941/chemotherapeutic-drugs/miss-madhu-rajput
Austin Journal of Molecular and Cellular Biology research is an international scholarly peer reviewed Open Access journal, aims to promote the research in Journal of Molecular and Cellular Biology, which is closely related to molecular biology, developmental biology, immunology, biochemistry, genetics, biological sciences, cancer research, cellular microbiology etc.,
Austin Journal of Molecular and Cellular Biology is a comprehensive Open Access peer reviewed scientific Journal that covers multidisciplinary fields. We provide limitless access towards accessing our literature hub with colossal range of articles. The journal aims to publish high quality varied article types such as Research, Review, Short Communications, Case Reports, Perspectives (Editorials), Clinical Images.
Austin Journal of Molecular and Cellular Biology supports the scientific modernization and enrichment in Journal of Molecular and Cellular Biology research community by magnifying access to peer reviewed scientific literary works. Austin also brings universally peer reviewed member journals under one roof thereby promoting knowledge sharing, collaborative and promotion of multidisciplinary science.
Natural Compounds as Adjuncts for Treating Colon Cancer through Apoptotic Pat...ijtsrd
Cancer is one of the deadliest diseases which is caused when abnormal cells divide uncontrollably and destroy body tissue. Colorectal cancer CRC , also known as colon cancer, is one of the most common type of cancers in humans is closely linked to the global cancer – related mortalities worldwide. The ongoing clinical treatment for colorectal cancer or colon cancer largely engages surgery and chemotherapy. But as there are many side effects and due to emergence of drug resistance, it has become very necessary to find novel and more effectual drugs for colon cancer treatment. Chemo prevention, a novel method for controlling cancer encompasses the use of specific natural products or synthetic chemical agents to reverse, suppress or prevent premalignancy before the development of invasive cancer. Many studies have signified that various natural products have efficacious anti CRC effects and may be used as substitutional chemotherapy agents for CRC therapy. Various natural compounds have been shown to be promising on the basis of their anti cancerous effects and low toxicity. In this review we summarise the natural compounds having anti CRC effects from distinct sources. This review suggest that they provide a novel opportunity for treatment of colon cancer. Sarita Negi | Nikita Kaushik | Runjhun Mathur | Abhimanyu Kumar Jha "Natural Compounds as Adjuncts for Treating Colon Cancer through Apoptotic Pathway" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-4 , June 2021, URL: https://www.ijtsrd.compapers/ijtsrd42387.pdf Paper URL: https://www.ijtsrd.compharmacy/biotechnology-/42387/natural-compounds-as-adjuncts-for-treating-colon-cancer-through-apoptotic-pathway/sarita-negi
This document is for those who have questions about CHED. Frequently Ask Questions about CHED. References (Websites) for each question is also included at the end of the slides. Hopefully this will help you.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
5. INTRODUCTION
● Apoptosis, also known as 'programmed cell
death', is a process by which cells self-destruct
in order to maintain homeostasis in the body. It
is an integral part of the body's natural defense
system, allowing the body to rid itself of cells
that are no longer needed or are potentially
harmful.
6. In simple term
● Apoptosis is a type of cell death in which a series
of molecular steps in a cell lead to its death. This
is one method the body uses to get rid of unneeded
or abnormal cells. The process of apoptosis may be
blocked in cancer cells. Also called programmed
cell death.
6
7. The Benefits of Apoptosis
● Apoptosis is an important process for
maintaining the health of the body. It helps
to rid the body of cells that are no longer
needed or are potentially harmful, and it also
helps to maintain homeostasis. It is also
important for the development of the body,
as it helps to shape organs and tissues.
8. The Role of Apoptosis in Cancer
● Apoptosis plays an important role in the
development and progression of cancer. In
healthy cells, apoptosis helps to maintain
homeostasis and rid the body of cells that are
no longer needed or are potentially harmful.
However, in cancer cells, apoptosis is inhibited,
allowing the cells to grow and spread
unchecked.
9. The Role of Apoptosis in Cancer
● Understanding the role of apoptosis in cancer is
important for developing treatments and
preventing the spread of the disease.
Additionally, understanding the process of
apoptosis can help researchers develop drugs
that target cancer cells and inhibit their growth.
10. Conclusion
● Apoptosis is an essential process for
maintaining the health of the body. Its failure
can lead to a variety of diseases, including
cancer. Understanding apoptosis is important
for developing treatments and preventing
diseases, as well as for understanding the role
of cell death inhibitors in treating disease.
11. References:
● Alberts B, Johnson A, Lewis J, et al. (2002). Molecular Biology of the Cell. 4th
edition. New York: Garland Science; 2002. Programmed Cell Death
(Apoptosis) Retrieved from:
https://www.ncbi.nlm.nih.gov/books/NBK26873/
● Jajoo, Aarti, PhD. (2023). Apoptosis. National Human Genome Research
Institute. Retrieved from https://www.genome.gov/genetics-
glossary/apoptosis#:~:text=Apoptosis%20is%20the%20process%20of,a
%20role%20in%20preventing%20cancer.
● Schlesinger, P. H. (2022, December 19). apoptosis. Encyclopedia Britannica.
Retrieved from https://www.britannica.com/science/apoptosis
Editor's Notes
Apoptosis is an orderly process in which the cell's contents are packaged into small packets of membrane for “garbage collection” by immune cells. Apoptosis removes cells during development, eliminates potentially cancerous and virus-infected cells, and maintains balance in the body.
Apoptosis is a tightly regulated process, and its failure can lead to a variety of diseases, including cancer. Understanding apoptosis is important for developing treatments and preventing diseases.
Apoptosis is a tightly regulated process, and its importance cannot be overstated. Understanding apoptosis is essential for developing treatments and preventing diseases, as well as for understanding the role of cell death inhibitors in treating disease.