Screening Methods of Anti-inflammatory Drugs ... outline , Mechanism and some drugs

1. Screening of Anti-inflammatory
Drugs
Dr. B. R. Ambedkar University,
Agra-282002
Department of Pharmacy
Presented By
Krishna kumar
B. Pharma IV Sem
E-mail: krishnaworldpharma@gmail.com

2. 14/05/17 Krishna Kumar
Outline
• Describe the inflammatory response
• Anti-inflammatory drugs; MOA, Uses, SE
• Explain methods for screening of Anti-
inflammatory drugs
• Discuss the lab work

3. 14-05-17 Krishna Kumar
Inflammation
• Response of immune system to foreign organism
or antigenic substance
• Characterized by edema, erythema, swelling, and
pain or tenderness

4. 14-05-17 Krishna Kumar
Inflammatory Mediators
Phospholipids
http://nnsearch.qsrch.com/shp.php

5. 14-05-17 Krishna Kumar
Phases of inflammation
• Acute: vasodilation & increase capillary
permeability
• Delayed: infiltration of leukocytes and phagocyte
cells
• Chronic proliferative: tissue degeneration and
fibrosis

6. 14-05-17 Krishna Kumar
Anti-inflammatory Drugs
• Steroidal: ex. Corticosteroids
(inh. gene transcription )
• Non-steroidal Anti-inflammatory Drugs (NSAID)
(inh. COX)
Examples: ASA, indomethacin, ibuprofen
• Selective COX-2 inhibitors

7. 14-05-17 Krishna Kumar
NSAID
• Uses: rheumatoid arthritis
• SE: GI adverse effects, renal insufficiency,
bronchospasm

8. 14-05-17 Krishna Kumar
Screening methods For Anti-
inflammatory Drugs
• Granuloma Pouch edema methods
• Croton oil
• Control vs. treated
• Calculate % inhibition
• Adjuvant-induced arthritis
• ID Tubercle bacilli
• Control vs. treated
• Calculate % change in edema & body wt. over 14
days

9. 14-05-17 Krishna Kumar
Screening methods For Anti-
inflammatory Drugs
• Rat-paw edema
• Animal 6 rats (3 control, 3 treated)
• Drugs: carragenin (2%, 0.1 ml SC.), indomethacin 2
mg/kg IP

10. 14-05-17 Krishna Kumar
Cont.
Control group Treated group
Rt. Lt. Rt. Lt.
1 2.9 1.5 1.9 1.3
2 3.1 1.3 1.7 1.4
3 3 1.6 2 1.6
Mean A B C D

11. 14-05-17 Krishna Kumar
Calculation
• % of edema produced by carragenin alone
= A – B X 100 = X %
B
• % of edema produced by carragenin after ttt with indomethacin
= C – D X 100 = Y %
D
• % reduction of edema by indomethcin
= X –Y= %

12. •Thank You
•THE END
14-05-17 Krishna Kumar