Overview of Israel's leading BioFoundry, Alagene by Roni Cohen, CEO. Overview of Synthetic Biology- Taking biology into engineering mode. Using synthetic genes, to program cells to become factories to make devices, sensors, pharmaceuticals, renewable chemicals, fuels and food. Alagene has four partners with complementary expertise. Hylabs is an established and experienced, highly regulated service company. Reichman University is an academic partner with a commercialization mindset. The Israel Innovation Authority is the government and Aleph VC is an investment firm. Alagene is a home for innovation and R&D center in SynBio, serving as a one-stop—shop for knowledge, methods, and instrumentation to enable our customers in Israel and worldwide to get to their proof of concept. Alagene is anend to end Infrastructure supporting gene & host optimization in microbial systems. The infrastructure enables two Major Implementations- Biomanufacturing and Direct Microbial Application. One example is the development of a Dairy Milk Producing System, using Genetic Optimization and Process Optimization and Validation. Finally, Alagene shared the following recent project examples: Computational prediction for introducing new genes into microbes; Precision fermentation for yeast producing dairy proteins; Bio production of psychoactive / cannabinoid molecule in yeast; Bio production of growth factors for the cultured meat industry; Developing new inhouse tools to support strain optimization; Bio production of egg protein; Computational modeling of full metabolic network in Algae; Bio production of rare sugars with a low glycemic index.

1. Feb. 2023
The Israeli BioFoundry
Releasing the Genie Out of the Bottle

2. Synthetic Biology: Taking biology into engineering mode

3. The Needs
Those who want to venture into the field
of SynBio currently can’t, due to:
• Lack of physical infrastructure and professional equipment
• High equipment and construction costs
• Lack of professional support and guidance
• Lack of scientific resources and ecosystem
• Lack of a customer-oriented service center

4. A Unique Venture with Complementary Expertise
Academic partners with a
commercialization mindset
Established & experienced, highly
regulated service company
Michael
Eisenberg
Israel’s leader in venture capital and
high-growth startups

5. Our Vision
Engineering life for the
benefit of humanity
Alagene is a home for innovation and R&D center in SynBio, serving as a
one-stop—shop for knowledge, methods, and instrumentation to enable our customers in
Israel and worldwide to get to their proof of concept.

6. An End-To-End Solution
Infrastructure supporting gene & host
optimization in microbial systems
Computational
Design
Construct & Host
Engineering
Optimization by
Automation &
Analytics
Precision Fermentation,
Pre-Production & POC

7. Enabler of Two Major Implementations
Zhang et al.,
2022
 56 genetic edits
 34 heterologous genes
Production of Anti-Cancerous
2ndary Plant Metabolite in
Yeast
Scalable platform
 >3,000 natural alkaloids
 Infinite number analogues
Market Sectors
 Therapeutics
 Agritech
 Cleantech &
safety
 Security
 Materials
 Others
Biomanufacturing – Going Wild! Direct Microbial Application – Sky’s the Limit!

8. Host
Engineering
Random
Mutagenesis
Cell Sorting
(Selection)
Cell Screening
Mating &
Sporulation
Precision
Fermentation
Development of a Dairy Milk Producing
System
Rational
Design
• Induction
• PTM
Directed Evo.
• Copy number
3 iterations
Objective: fermentation yield of ≥1 g/L
Alagene designed the following SoW
Assay setup &
calibration
Assay setup &
calibration in
collaboration
with
specialized
academic group
Assay setup &
calibration
Assay setup &
calibration
Assay setup &
calibration
Genetic Optimization Process Optimization

9. Gene Optimization
& Host Engineering
Protein
Purification
& Preparation
Cell Screening &
Analysis
Bioactivity
Precision
Fermentation
Genetic Optimizatiom Process Optimization
 Codon
optimization
 Expression
module
 Auxiliary
mutations
 Assay setup &
calibration
 Screening by
yield  Process setup &
calibration
 QC
Validation
 Assay setup &
calibration
SynBio project cycle

10. A few project examples:
 Bio production of egg protein
 Computational modeling of full metabolic
network in Algae
 Bio production of rare sugars with a low
glycemic index
 Computational prediction for introducing
new genes into microbes
 Precision fermentation for yeast producing
dairy proteins
 Bio production of psychoactive /
cannabinoid molecule in yeast
 Bio production of growth factors for the
cultured meat industry
 Developing new inhouse tools to support
Industry: Academia:

11. Thank You