1. A STUDY ON INCIDENCE OF
AFLATOXIGENIC FUNGI AND
AFLATOXIN CONTAMINATION
IN OIL SEEDS
Dr.Daxaben N. Mehta
Principal
Smt.Sadguna C.U.Shah Home Science and
C.U.Shah Arts & Commerce Mahila college
WADHWANCITY:363030

2. INTRODUCTION
AFLATOXIN - A Secondary Toxic Fungal Metabolite
A.flavus and A.parasiticus
Potent Hepatotoxic, Carcinogenic, Mutagenic
Peanut and Cotton - high risk substrates

3. MATERIALS AND METHODS
Samples of oil seeds
Parts of Surendranagar district
blotter test, agar plate and dilution plate method
Percentage of Incidence, Frequency and Abundance
Aflatoxin producing potentials - SMKY liquid medium
Culture filtrates and oilseeds -BGYF

4. RESULTS
Incidence of Fungi
21 different fungi were isolated from the
samples of peanut, cotton, sesamum and
castor seeds. Amongst them
A.niger, A. flavus, other Aspergilli,
Penicillia, Rhizopus and Mucor species
were most frequently encountered

5. Myco floras of Peanut seeds
Nine fungi isolated from peanut seeds
Were A.niger, A.flavus, Aspergillus sp.,
Penicillium sp., Mucor sp., Rhizopus sp.,
Fusarium moniliformae, Helminthosporium sp.
and Curvularia lunata.
Amongst these A.niger and Aspergillus sp.
were found in samples of all the localities.

6. Myco floras of Cotton Seeds
Eleven fungi isolated from cotton seeds
were A.niger, A.flavus, A.tamarii, A.ochraceus,
Aspergillus sp., Penicillium sp., Mucor sp.,
Rhizopus sp., Fusarium moniliformae,
Fusarium oxysporum, and Ustilago sp.,
amongst all A. flavus and Aspergillus sp.
were invariably found in all the samples

7. Myco floras of Castor Seeds
Seven fungi isolated from castor seeds were
A.niger, A.flavus, A. ochraceus,
A.candidus, Aspergillus sp., Penicillium sp.,
Mucor sp. Amongst these A.niger, A. flavus,
Aspergillus sp. Penicillium sp., Mucor sp.
were recovered from all the samples.

8. Myco floras of Sesamum Seeds.
Eighteen fungi isolated from sesamum seeds
include A. niger, A. flavus, A. tamarii, A. ochraceus,
Aspergillus sp., P.citrinum, P.expansum, Penicillium sp.,
Mucor sp., Rhizopus sp., F.solani, F. oxysporum,
F. moniliformae, Curvularia sp., Alternaria sp.,
Helminthosporium sp., Rhizoctonia solani and
Cladosporium sp. Amongst these A.niger and A. ochrece
were recovered from all the samples.

9. Aflatoxigenic Fungi
Total 52 isolates of A. flavus and A. parasiticus
were recovered from oil seed samples
collected from different localities. Amongst these,
11 isolates were found to be toxigenic.
Peanut seeds amongst all four oil seeds
studied were found to have higher
aflatoxin producing potential .

10. Aflatoxin Content
Four different types of oil seeds collected were
analyzed for their natural aflatoxin contamination.
Peanut seeds were found to have more aflatoxin
contents. Aflatoxin B1 , B2 , G1 and G2 were
recorded in peanut and sesamum seeds while only
B1 , B2 and G1 recorded from castor seeds. From
cotton seeds B1 along with G1 was recorded

11. Discussion
The type of fungi and the degree of contamination
varied with the environmental conditions. The oil
seeds were more infested than oil cakes. The screening of
fungi from various oil seeds revealed that some fungi
are toxigenic and may be a health hazard to livestock
and in turn to man. Although some isolates were found
to produce aflatoxin under cultural conditions, the
production of aflatoxin under natural conditions is limited.
This could be due to Unfavorable and changing conditions
as well as the interactions of other micro-organisms may
in turn affect the aflatoxin production.

12. O U
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