This research report summarizes acoustic emission (AE) testing conducted on four prestressed concrete bridge girders on I-565 in Huntsville, Alabama. The testing was performed to evaluate AE evaluation criteria for assessing the structural integrity of in-service prestressed concrete bridges. Both pre- and post-fiber reinforced polymer (FRP) repair tests were conducted. The evaluation methods used were the NDIS-2421 criterion, signal strength moment ratio analysis, and AE 2D location analysis. It was concluded that while the testing procedure provided results efficiently, the evaluation criteria needed adjustment for testing prestressed concrete bridge girders in service.