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International Journal of Trend in Scientific Research and Development (IJTSRD)
Volume 7 Issue 5, September-October 2023 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470
@ IJTSRD | Unique Paper ID – IJTSRD59916 | Volume – 7 | Issue – 5 | Sep-Oct 2023 Page 382
A Review Article on Soft Embalming
Methods in Ayurveda and Modern Science
Dr. Piyushlata Maheshwari
Professor, Department of Rachna Sharir, Poornayu Ayurved Chikitsalaya
Evam Anusandhan Vidhyapeeth, Jabalpur, Madhya Pradesh, India
ABSTRACT
Rachna sharir is basic concept of Ayurveda and Acharya Sushrut was
the first surgeon who discovered the method of preservation of body
for dissection. Description of various internal structures which are
limited externally by skin can be available for practical
demonstrations only by dissection. An expert surgeon who wants to
have a detailed knowledge of the sharira should preserve and dissect
the body. The subject studied in literature, should be experienced in
Practical. These two methods are complimentary to each other and
enhance the entire knowledge. Sharir is defined as "Shiryate tat
shariram". Meaning a Sharir is "a physiological entity where
continuous process of degeneration is carried out itself". A sharir
can't be preserved and available after death. But embalming made the
sharir available to dissect after death.
KEYWORDS: Dissection, Human cadaver, Mritsanrakshan,
Embalming
How to cite this paper: Dr. Piyushlata
Maheshwari "A Review Article on Soft
Embalming Methods in Ayurveda and
Modern Science" Published in
International
Journal of Trend in
Scientific Research
and Development
(ijtsrd), ISSN:
2456-6470,
Volume-7 | Issue-5,
October 2023,
pp.382-384, URL:
www.ijtsrd.com/papers/ijtsrd59916.pdf
Copyright © 2023 by author (s) and
International Journal of Trend in
Scientific Research and Development
Journal. This is an
Open Access article
distributed under the
terms of the Creative Commons
Attribution License (CC BY 4.0)
(http://creativecommons.org/licenses/by/4.0)
INTRODUCTION
Acharya Sushrut defined sharir as "Combined shukra
and shonita in the womb mixed with eight prakriti
and sixteen vikar, ridden in by Atma is garbha and is
further divided by vayu, metabolized by Teja,
bewetted by apa, condensed by Prithvi and developed
by Akasha. Acharya Charak asserted sharir is a
biological unit of conglomeration of the derivates of
Panchamahabhutas clasping the soul and it is the site
of manifestation of consciousness".
Panchamahabhutas are interrelated in a proper media
and they are present in the state of inseparable
cohesion during life. Acharya Sushrut has a vast
knowledge of sharir i.e. human body, its rachna and
surgery in that ancient time. He discovered this
information by both inspection as well as dissection
as he believed that a physician can only become
perfect after having complete practical and theoretical
knowledge. In spite of all restrictions in Indian
society and the dead body is considered as
untouchable and sacred acharya Sushrut was able to
overcome the problem and made the cadaver
available for the study of students and also discovered
the method of Mritasanrakshan to teach his students.
Acharya Shushruta says that what we learn from our
samhitas and teacher should be confirmed by
practical experiments. A physician can only become
perfect after having complete practical and theoretical
knowledge. The subject studied in literature, should
be experienced in Practical. These two methods are
complimentary to each other and enhance the entire
knowledge. An expert surgeon who wants to have a
detailed knowledge of the sharira should preserve and
dissect the body.
A body for the purpose of dissection should be
selected with features as1
 A body with all organs and parts undetached,
 Death is not due to poison
 Death not due to suffering from chronic disease.
 Dead body should not be too old i.e. approaching
the age of 100 years
 Faecal matter should be removed from intestines
of the body.
IJTSRD59916
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD59916 | Volume – 7 | Issue – 5 | Sep-Oct 2023 Page 383
Method of preservation2:
For preservation, this cadaver is kept in a cage tied
with Munja, Valkala, Kusha or Shana and kept
immersed in slowly but continuously flowing water in
a dark place to protect it from animals. And also
water from that resource is not consumed by humans.
After seven days, confirming the softening of body
tissues, the body is dissected by kurch yantras made
of Usheera, Venu, Twak and Baala. Rub the body
surface slowly to visualize all the internal and
external structures properly. Acharya advised to avoid
sharp instruments for dissection.
Here the preservation aimed and focused on the
softening of cadaver and then rubbing the surface
with blunt instruments and not using sharp
instruments to cut and dissecting it.
Modern Era3:
Teaching of anatomy of human body is an integral
part of study of Ayurveda, medicine, dentistry and all
paramedical sciences. In modern era study of
anatomy has to be carried out on the dead, preserved
human body called cadaver in which texture and
appearance of the structures and organs of the body
has not been altered. The word cadaver is derived
from the Latin word "Cadre" means "to fall".
Dissection of a well preserved human cadaver is a
best method of learning anatomy of different parts of
the body. The dissection is a time consuming process
and the body remains exposed for several months
without dehydration and decomposition. The
dissected parts of the body are displayed in the
museum and kept for years. Therefore, proper
preservation of the cadaver is necessary to understand
the structural integrity and topography of human
body.
Traditionally most of the anatomy departments use
formaldehyde based embalming methods. Though,
Formalin provide a long term preservation of
structures as it is bactericidal, fungicidal, and
insecticidal and has excellent antiseptic properties;
however it causes over hardening, drying, extreme
rigidity and has an unpleasant odor. Along with it also
has health hazards. The formalin based embalming
has been used for decades in spite of the concerns
related to its contribution to various health issues like
skin allergies, irritation in eyes and throat and even
asthma and cancer are detected in long term exposure.
Cost effectiveness, efficiency of preservation and
sustainability are the major factors for continued use
of this technique. However this traditional formalin
fixation has been found to be unsuitable for the
purpose of surgical skills training due to lack of
flexibility, elasticity of tissues and low preciseness.
The fresh frozen cadavers though offer a good
alternative for surgical skills training but they
deteriorate faster due to repeated thawing, can be
used for short term only and carry a significant risk of
infection.
In 1990 Professor Walter Thiel developed an
embalming technique that produced life like soft and
flexible cadavers and the procedure since then called
Thiel soft fix embalming.
Soft embalming is an alternative to the traditional
based preservation method consisting of water based
mixtures, like
 Propylene glycol
 ammonium nitrate
 potassium nitrate
 sodium sulfite
 boric acid
 chlorocresol
 low amounts of formalin
 Along with alcohol and morpholine.
The cadaver thus produced are not only soft, pliable,
flexible but can be kept for longer time and are
suitable for surgical skill training procedures. The
embalming process comprises of an initial perfusion
followed by immersion in immersion fluid for at least
two months. After that body is stored in plastic bag
and kept in freezers at - 4c. The cadaver is embalmed
by arterial infusion into carotid or femoral artery. The
sloughing of keratin layer of skin marks the end point
of the infusion. This preservation is effective in long
term, with estimated viability of 36 months. Thus,
this method of preservation allows multiple uses of
cadavers, increasing availability and reducing the cost
incurred.
ROLE OF VARIOUS CONSTITUENTS OF
SOFT EMBALMING FLUID4
 Formaldehyde: It is bactericidal, fungicidal and
insecticidal. It destroys putrefactive organisms
when dissolved in a vehicle which allows it to
permeate the organisms.
 Boric Acid: It works as insecticide or
bacteriostatic and is responsible for modification
of the integrity and alignment of muscle fiber.
 Phenol /Carbolic acid: It is used as a
disinfectant, acts as excellent fungicide and
bactericide; although it causes discoloration and
dryness of tissues. Liquefied phenol is very
effective in preventing molding.
 4-Chloro-3-methylphenol: It is used as an
antiseptic and preservative agent. It is highly
soluble and remains active over a wide range of
pH.
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD59916 | Volume – 7 | Issue – 5 | Sep-Oct 2023 Page 384
 Ethyl glycol: It is used to preserve the moisture
and tissue Plasticity in preserved cadaver.
 Ammonium nitrate, Potassium nitrate and
Sodium sulfite: They absorb the water content of
the tissues. The nitrate gives red color to the
muscles
 Alcohols: It have bactericidal and bacteriostatic
and also have antiviral, antifungal and
antimycosal effects by acting on proteins and
denaturing them.
The consistency, color and tactile feedback of
procedures done on Thiel soft fix embalmed cadavers
are reported to be almost identical to live procedures.
They have been used for various skills training in
surgery. They form a useful resource for surgical
training in many specialties like gynecology,
otolaryngology, anesthesia, orthopedics and oral
surgery.
Advantages of Thiel soft fix embalmed cadaver
1. Excellent flexibility and tissue quality.
2. High standards of preservation without releasing
harmful substances
3. Easy identification of muscles
4. Increased range of joint movements
Disadvantages of Thiel soft fix embalmed cadaver
1. High cost
2. Short lasting
3. Muscular disintegration
4. Difficult to handle due to increased pliability
5. Loss of anatomical impressions and landmarks.
Logan described a method where he used alcohol,
glycerine, phenol, formaldehyde. Coleman and
Kogan modified it by adding high component of salt
(NaCl) and replaced alcohol with isopropyl alcohol.
Glutaraldehyde embalming5:
The main component of this embalming fluid is
Glutaraldehyde, first shown by Nateker and De Souza
to be used in preservation for surgical dissection. The
embalmed fluid was modified by adding 2%
glutaraldehyde, methanol, glycerine, cetrimide, eosin,
eucalyptus oil and water. Cadavers thus preserved are
found to be more pliable and realistic; all joints were
flexible, with no evidence of fungal infection. The
cadaver was used several times without refreezing for
five years.
Formaldehyde free embalming solution6:
It included ethyl alcohol 25%, polyethylene glycol
20%, chloroxylenol 0.1% and sodium nitrate 10%
was made 100% with water base. The embalmed
specimen resembled living specimen and also there
were no microbial activity over six months.
CONCLUSION:
Since the ancient time, as Maharshi Sushrut did
thousands of years before the soft embalming was
preferred that time and also today in present era. In
this article many soft embalming methods has been
discussed from ancient times to modern era. In
Ayurveda the method of preservation was natural,
simple and without any harmful effect but it is
unlogistic in present era .Thiel embalming technique
is found to be best in many parts of world but it is not
so success in tropical countries like India
An ideal embalming fluid
 Should ensure that there is no risk of infection on
contact with cadaver
 Should produce natural life like appearance to the
body
 Should prevent contamination with molds,
maggots, fungus and insects
 Should ensure prevention of putrification in body
 Should not have any unpleasant effect on skin and
other organs on prolonged contact as formalin
has.
ACKNOWLEDGEMENT
[1] Sushrut Samhita of Maharshi Sushrut, hindi
Sanskrit commentary by Sansthan, Varanasi,
Kaviraj Ambikadatta Shastri, part-1,
Chaukhamba reprint edition, sharir sthana;
2021;5/61:66
[2] Embalming,Principles and Legal Aspects by M
L Ajmani, Jaypee Brothers Medical Publishers
Ltd; II editition; 2019; 21;137-139
[3] Raghuveer Reddy, Suramania Iyer, Minnie
Pillay, Krishna kumar Thankappan and
Janartanan Ramu, Soft embalming of cadavers
for training purposes: Optimising for long term
use in tropical weather,2017, Indian journal of
plastic surgery, {PuBMeD} {Google Scholar}

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A Review Article on Soft Embalming Methods in Ayurveda and Modern Science

  • 1. International Journal of Trend in Scientific Research and Development (IJTSRD) Volume 7 Issue 5, September-October 2023 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470 @ IJTSRD | Unique Paper ID – IJTSRD59916 | Volume – 7 | Issue – 5 | Sep-Oct 2023 Page 382 A Review Article on Soft Embalming Methods in Ayurveda and Modern Science Dr. Piyushlata Maheshwari Professor, Department of Rachna Sharir, Poornayu Ayurved Chikitsalaya Evam Anusandhan Vidhyapeeth, Jabalpur, Madhya Pradesh, India ABSTRACT Rachna sharir is basic concept of Ayurveda and Acharya Sushrut was the first surgeon who discovered the method of preservation of body for dissection. Description of various internal structures which are limited externally by skin can be available for practical demonstrations only by dissection. An expert surgeon who wants to have a detailed knowledge of the sharira should preserve and dissect the body. The subject studied in literature, should be experienced in Practical. These two methods are complimentary to each other and enhance the entire knowledge. Sharir is defined as "Shiryate tat shariram". Meaning a Sharir is "a physiological entity where continuous process of degeneration is carried out itself". A sharir can't be preserved and available after death. But embalming made the sharir available to dissect after death. KEYWORDS: Dissection, Human cadaver, Mritsanrakshan, Embalming How to cite this paper: Dr. Piyushlata Maheshwari "A Review Article on Soft Embalming Methods in Ayurveda and Modern Science" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-7 | Issue-5, October 2023, pp.382-384, URL: www.ijtsrd.com/papers/ijtsrd59916.pdf Copyright © 2023 by author (s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0) INTRODUCTION Acharya Sushrut defined sharir as "Combined shukra and shonita in the womb mixed with eight prakriti and sixteen vikar, ridden in by Atma is garbha and is further divided by vayu, metabolized by Teja, bewetted by apa, condensed by Prithvi and developed by Akasha. Acharya Charak asserted sharir is a biological unit of conglomeration of the derivates of Panchamahabhutas clasping the soul and it is the site of manifestation of consciousness". Panchamahabhutas are interrelated in a proper media and they are present in the state of inseparable cohesion during life. Acharya Sushrut has a vast knowledge of sharir i.e. human body, its rachna and surgery in that ancient time. He discovered this information by both inspection as well as dissection as he believed that a physician can only become perfect after having complete practical and theoretical knowledge. In spite of all restrictions in Indian society and the dead body is considered as untouchable and sacred acharya Sushrut was able to overcome the problem and made the cadaver available for the study of students and also discovered the method of Mritasanrakshan to teach his students. Acharya Shushruta says that what we learn from our samhitas and teacher should be confirmed by practical experiments. A physician can only become perfect after having complete practical and theoretical knowledge. The subject studied in literature, should be experienced in Practical. These two methods are complimentary to each other and enhance the entire knowledge. An expert surgeon who wants to have a detailed knowledge of the sharira should preserve and dissect the body. A body for the purpose of dissection should be selected with features as1  A body with all organs and parts undetached,  Death is not due to poison  Death not due to suffering from chronic disease.  Dead body should not be too old i.e. approaching the age of 100 years  Faecal matter should be removed from intestines of the body. IJTSRD59916
  • 2. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD59916 | Volume – 7 | Issue – 5 | Sep-Oct 2023 Page 383 Method of preservation2: For preservation, this cadaver is kept in a cage tied with Munja, Valkala, Kusha or Shana and kept immersed in slowly but continuously flowing water in a dark place to protect it from animals. And also water from that resource is not consumed by humans. After seven days, confirming the softening of body tissues, the body is dissected by kurch yantras made of Usheera, Venu, Twak and Baala. Rub the body surface slowly to visualize all the internal and external structures properly. Acharya advised to avoid sharp instruments for dissection. Here the preservation aimed and focused on the softening of cadaver and then rubbing the surface with blunt instruments and not using sharp instruments to cut and dissecting it. Modern Era3: Teaching of anatomy of human body is an integral part of study of Ayurveda, medicine, dentistry and all paramedical sciences. In modern era study of anatomy has to be carried out on the dead, preserved human body called cadaver in which texture and appearance of the structures and organs of the body has not been altered. The word cadaver is derived from the Latin word "Cadre" means "to fall". Dissection of a well preserved human cadaver is a best method of learning anatomy of different parts of the body. The dissection is a time consuming process and the body remains exposed for several months without dehydration and decomposition. The dissected parts of the body are displayed in the museum and kept for years. Therefore, proper preservation of the cadaver is necessary to understand the structural integrity and topography of human body. Traditionally most of the anatomy departments use formaldehyde based embalming methods. Though, Formalin provide a long term preservation of structures as it is bactericidal, fungicidal, and insecticidal and has excellent antiseptic properties; however it causes over hardening, drying, extreme rigidity and has an unpleasant odor. Along with it also has health hazards. The formalin based embalming has been used for decades in spite of the concerns related to its contribution to various health issues like skin allergies, irritation in eyes and throat and even asthma and cancer are detected in long term exposure. Cost effectiveness, efficiency of preservation and sustainability are the major factors for continued use of this technique. However this traditional formalin fixation has been found to be unsuitable for the purpose of surgical skills training due to lack of flexibility, elasticity of tissues and low preciseness. The fresh frozen cadavers though offer a good alternative for surgical skills training but they deteriorate faster due to repeated thawing, can be used for short term only and carry a significant risk of infection. In 1990 Professor Walter Thiel developed an embalming technique that produced life like soft and flexible cadavers and the procedure since then called Thiel soft fix embalming. Soft embalming is an alternative to the traditional based preservation method consisting of water based mixtures, like  Propylene glycol  ammonium nitrate  potassium nitrate  sodium sulfite  boric acid  chlorocresol  low amounts of formalin  Along with alcohol and morpholine. The cadaver thus produced are not only soft, pliable, flexible but can be kept for longer time and are suitable for surgical skill training procedures. The embalming process comprises of an initial perfusion followed by immersion in immersion fluid for at least two months. After that body is stored in plastic bag and kept in freezers at - 4c. The cadaver is embalmed by arterial infusion into carotid or femoral artery. The sloughing of keratin layer of skin marks the end point of the infusion. This preservation is effective in long term, with estimated viability of 36 months. Thus, this method of preservation allows multiple uses of cadavers, increasing availability and reducing the cost incurred. ROLE OF VARIOUS CONSTITUENTS OF SOFT EMBALMING FLUID4  Formaldehyde: It is bactericidal, fungicidal and insecticidal. It destroys putrefactive organisms when dissolved in a vehicle which allows it to permeate the organisms.  Boric Acid: It works as insecticide or bacteriostatic and is responsible for modification of the integrity and alignment of muscle fiber.  Phenol /Carbolic acid: It is used as a disinfectant, acts as excellent fungicide and bactericide; although it causes discoloration and dryness of tissues. Liquefied phenol is very effective in preventing molding.  4-Chloro-3-methylphenol: It is used as an antiseptic and preservative agent. It is highly soluble and remains active over a wide range of pH.
  • 3. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD59916 | Volume – 7 | Issue – 5 | Sep-Oct 2023 Page 384  Ethyl glycol: It is used to preserve the moisture and tissue Plasticity in preserved cadaver.  Ammonium nitrate, Potassium nitrate and Sodium sulfite: They absorb the water content of the tissues. The nitrate gives red color to the muscles  Alcohols: It have bactericidal and bacteriostatic and also have antiviral, antifungal and antimycosal effects by acting on proteins and denaturing them. The consistency, color and tactile feedback of procedures done on Thiel soft fix embalmed cadavers are reported to be almost identical to live procedures. They have been used for various skills training in surgery. They form a useful resource for surgical training in many specialties like gynecology, otolaryngology, anesthesia, orthopedics and oral surgery. Advantages of Thiel soft fix embalmed cadaver 1. Excellent flexibility and tissue quality. 2. High standards of preservation without releasing harmful substances 3. Easy identification of muscles 4. Increased range of joint movements Disadvantages of Thiel soft fix embalmed cadaver 1. High cost 2. Short lasting 3. Muscular disintegration 4. Difficult to handle due to increased pliability 5. Loss of anatomical impressions and landmarks. Logan described a method where he used alcohol, glycerine, phenol, formaldehyde. Coleman and Kogan modified it by adding high component of salt (NaCl) and replaced alcohol with isopropyl alcohol. Glutaraldehyde embalming5: The main component of this embalming fluid is Glutaraldehyde, first shown by Nateker and De Souza to be used in preservation for surgical dissection. The embalmed fluid was modified by adding 2% glutaraldehyde, methanol, glycerine, cetrimide, eosin, eucalyptus oil and water. Cadavers thus preserved are found to be more pliable and realistic; all joints were flexible, with no evidence of fungal infection. The cadaver was used several times without refreezing for five years. Formaldehyde free embalming solution6: It included ethyl alcohol 25%, polyethylene glycol 20%, chloroxylenol 0.1% and sodium nitrate 10% was made 100% with water base. The embalmed specimen resembled living specimen and also there were no microbial activity over six months. CONCLUSION: Since the ancient time, as Maharshi Sushrut did thousands of years before the soft embalming was preferred that time and also today in present era. In this article many soft embalming methods has been discussed from ancient times to modern era. In Ayurveda the method of preservation was natural, simple and without any harmful effect but it is unlogistic in present era .Thiel embalming technique is found to be best in many parts of world but it is not so success in tropical countries like India An ideal embalming fluid  Should ensure that there is no risk of infection on contact with cadaver  Should produce natural life like appearance to the body  Should prevent contamination with molds, maggots, fungus and insects  Should ensure prevention of putrification in body  Should not have any unpleasant effect on skin and other organs on prolonged contact as formalin has. ACKNOWLEDGEMENT [1] Sushrut Samhita of Maharshi Sushrut, hindi Sanskrit commentary by Sansthan, Varanasi, Kaviraj Ambikadatta Shastri, part-1, Chaukhamba reprint edition, sharir sthana; 2021;5/61:66 [2] Embalming,Principles and Legal Aspects by M L Ajmani, Jaypee Brothers Medical Publishers Ltd; II editition; 2019; 21;137-139 [3] Raghuveer Reddy, Suramania Iyer, Minnie Pillay, Krishna kumar Thankappan and Janartanan Ramu, Soft embalming of cadavers for training purposes: Optimising for long term use in tropical weather,2017, Indian journal of plastic surgery, {PuBMeD} {Google Scholar}