The document provides preparatory questions for a diffusion and osmosis lab. It asks about safety procedures, definitions of diffusion and osmosis, factors that affect diffusion, properties of differentially permeable membranes, how nonpolar and polar molecules pass through cell membranes, how molecules pass through dialysis tubing, colors of phenolphthalein and iodine solutions, differences between osmosis and diffusion, and concepts like hypertonic, hypotonic, isotonic, water potential, and effects of hypertonic IV solutions.
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1. Thursday 9/19 1
(9)Before you arrive for the Diffusion and Osmosis lab, please
1. Read the lab thoroughly, noting all safety guidelines.
2. Answer these preparatory questions:
What safety procedures should you be sure to follow during this lab period?
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What causes Brownian movement?_______________________________________
Define diffusion?______________________________________________________
Name the factors that affect diffusion, and identify the factor that is most
important in living things.
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Why are biological membranes and dialysis tubing considered to be differentially
permeable?
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Why would a nonpolar molecule pass across a cell membrane more readily than a
polar molecule? Recall the “fluid mosaic model” of membrane structure as you
answer this question.
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Which property of a molecule allows it to pass through dialysis tubing, while
other molecules can not pass through the dialysis tubing?
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What color is phenolphthalein in a basic solution?__________________________
2. Thursday 9/19 2
What color is produced when iodine is combined with starch?________________
What is the difference between osmosis and diffusion?______________________
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Consider the beaker and model cell above.
Both the cell and environment (beaker) contain sucrose solutions.
The model cell is impermeable to sucrose, but is permeable to water.
Is the cell hypertonic, hypotonic, or isotonic to the solution in the beaker?______
Will the cell shrink or swell?_____________________________________________
By what process will this occur?__________________________________________
What is water potential?________________________________________________
If water potential in an environment is higher than its surroundings, does water
flow into or out of that environment?
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Imagine some cut flowers sitting in water. For water to move through the stems
to the upper leaves, where must water potential be higher--in the stem, or in the
leaves?
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95% water
20%
Sucrose
3. Thursday 9/19 3
If you were given an IV solution in the hospital that was hypertonic to your blood,
what could happen to your red blood cells?
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