This study examined the relationship between role ambiguity, job stress, and job satisfaction among employees in the banking sector of Pakistan. A survey was administered to 350 employees of banks, with a response rate of 87%. The results found that role ambiguity had a negative relationship with job satisfaction, and that job stress played a mediating role between role ambiguity and job satisfaction. Specifically, role ambiguity led to increased job stress, which in turn led to lower job satisfaction. The findings suggest that controlling role stressors can help reduce job stress and increase employee job satisfaction in the banking sector.