Deoxyribonucleic acid is a molecule composed of two polynucleotide chains that coil around each other to form a double helix carrying genetic instructions for the development, functioning, growth and reproduction of all known organisms and many viruses.
details of the eukaryotic chromosome with the condensation of chromatin material during cell division. It is useful for the students studying cell and molecular biology and genetics at PG level.
details of the eukaryotic chromosome with the condensation of chromatin material during cell division. It is useful for the students studying cell and molecular biology and genetics at PG level.
A chromosome is a long DNA molecule with part or all of the genetic material of an organism. Most eukaryotic chromosomes include packaging proteins called histones which, aided by chaperone proteins, bind to and condense the DNA molecule to maintain its integrity.
Chromatin is the complex combination of DNA and proteins that makes up chromosomes. It can be made visible by staining with specific techniques and stain (thus the name chromatin which literally means colored material). The major proteins involved in chromatin are histone proteins; although many other chromosomal proteins have prominent roles too. The functions of chromatin is to package DNA into smaller volume to fit in the cell, to strengthen the DNA to allow mitosis and meiosis and to serve as a mechanism to control gene expression and DNA replication.
this file includes the packaging of DNA in Prokaryotic and Eukaryotic cell, the difference between DNA and RNA, Semi-conservative mode of DNA replication, and the mechanism of DNA replication.
Basics of Undergraduate/university fellows
Nucleosome model of chromosome is proposed by ROGER KORNBERG (son of Arthur
Kornberg) in 1974.
It was confirmed and crystalised by P. Oudet et al., (1975).
Nucleosome is the lowest level of Chromosome organization in eukaryotic cells.
Nucleosome model is a scientific model which explains the organization of DNA and
associated proteins in the chromosomes.
Nucleosome model also explains the exact mechanism of the folding of DNA in
thenucleus.
It is the most accepted model of chromatin organization.
11 how cells read the genome :from DNA to Proteinsaveena solanki
How does the cell convert DNA into working proteins? The process of translation can be seen as the decoding of instructions for making proteins, involving mRNA in transcription as well as tRNA.
In molecular biology, DNA replication is the biological process of producing two identical replicas of DNA from one original DNA molecule. DNA replication occurs in all living organisms acting as the most essential part for biological inheritance.
A protein is an organic compound made up of small molecules called amino acids. There are 20 different amino acids commonly found in the proteins of living organisms. Small proteins may contain just a few hundred amino acids, whereas large proteins may contain thousands of amino acids
Both RNA and DNA are made of nucleotides and take similar shapes. Both contain five-carbon sugars, phosphate groups, and nucleobases (nitrogenous bases). They both play important roles in protein synthesis. DNA has the five-carbon sugar deoxyribose and RNA has the five-carbon sugar ribose, hence their names
Cell chemistry and Biosynthesis
catalysis and the use of energy by cells
We now know there is nothing in living organisms that disobeys chemical and physical laws. However, the chemistry of life is indeed of a special kind. First, it is based overwhelmingly on carbon compounds, whose study is therefore known as organic chemistry. Second, cells are 70 percent water, and life depends almost exclusively on chemical reactions that take place in aqueous solution. Third, and most importantly, cell chemistry is enormously complex: even the simplest cell is vastly more complicated in its chemistry than any other chemical system known. Although cells contain a variety of small carbon-containing molecules, most of the carbon atoms in cells are incorporated into enormous polymeric molecules—chains of chemical subunits linked end-to-end. It is the unique properties of these macromolecules that enable cells and organisms to grow and reproduce—as well as to do all the other things that are characteristic of life
It is at first sight difficult to accept the idea that each of the living creatures described in the previous chapter is merely a chemical system. The incredible diversity of living forms, their seemingly purposeful behavior, and their ability to grow and reproduce all seem to set them apart from the world of solids, liquids, and gases that chemistry normally describes. Indeed, until the nineteenth century it was widely accepted that animals contained a Vital Force—an “animus”—that was uniquely responsible for their distinctive properties.
As a sub-discipline of biology, cell biology is concerned with the study of the structure and function of cells. As such, it can explain the structure of different types of cells, types of cell components, the metabolic processes of a cell, cell life cycle and signaling pathways to name a few.
Here, we shall look at some of the major areas of cell biology including some of the tools used.
Cell biology is the study of cell structure and function, and it revolves around the concept that the cell is the fundamental unit of life. Focusing on the cell permits a detailed understanding of the tissues and organisms that cells compose.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Embracing GenAI - A Strategic ImperativePeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
2. Experimental demonstration that DNA is the genetic material. These experiments, carried out
in the 1940s, showed that adding purified DNA to a bacterium changed its properties and that
this change was faithfully passed on to subsequent generations. Two closely related strains of
the bacterium Streptococcus pneumoniae differ from each other in both their appearance
under the microscope and their pathogenicity. One strain appears smooth (S) and causes death
when injected into mice, and the other appears rough (R) and is nonlethal. (A) This experiment
shows that a substance present in the S strain can change (ortransform) the R strain into the S
strain and that this change is inherited by subsequent generations of bacteria. (B) This
experiment, in which the R strain has been incubated with various classes of biological
molecules obtained from the S strain, identifies the substance as DNA.
3.
4. • Each DNA Molecule That Forms a Linear
Chromosome
• Contain a Centromere, Two Telomeres, and
• Replication Origins
• basic functions are controlled by three types of
specialized nucleotide sequence in the DNA,
• Each of which binds specific proteins that guide
the machinery that replicates and segregates
chromosomes
5. • centromere, allows one copy of each
duplicated and condensed chromosome to be
pulled into each daughter cell when a cell
divides.
• A protein complex called a kinetochore forms
at the centromere and attaches the duplicated
chromosomes to the mitotic spindle,
– allowing them to be pulled apart
6. • The third specialized DNA sequence forms
telomeres, the ends of a chromosome.
• Telomeres contain repeated nucleotide
sequences that enable the ends of chromosomes
to be efficiently replicated.
• Telomeres also perform another function:
– the repeated telomere DNA sequences, together with
the regions adjoining them,
– form structures that protect the end of the
chromosome
– from being recognized by the cell as a broken DNA
molecule in need of repair.
7. The three DNA sequences required to produce a eucaryotic chromosome that can be replicated
and then segregated at mitosis. Each chromosome has multiple origins of replication, one
centromere, and two telomeres. Shown here is the sequence of events a typical chromosome
follows during the cell cycle. The DNA replicates in interphase beginning at the origins of
replication and proceeding bidirectionally from the origins across the chromosome.
In M phase, the centromere attaches the duplicated chromosomes to the mitotic spindle so that
one copy is distributed to each daughter cell during mitosis. The centromere also helps to hold
the duplicated chromosomes together until they are ready to be moved apart. The telomeres
form special caps at each chromosome end.
8. • Nucleosomes Are the Basic Unit of Eucaryotic
Chromosome Structure
• The proteins that bind to the DNA to form
eucaryotic chromosomes are traditionally
divided into two general classes:
– the histones and the nonhistone chromosomal
proteins.
• The complex of both classes of protein with
the nuclear DNA of eucaryotic cells is known
as chromatin.
9. • Histones are responsible for the first and most basic level of
chromosome organization, the nucleosome, which was discovered
in 1974.
• When interphase nuclei are broken open very gently and their
contents examined under the electron microscope, most of the
chromatin is in the form of a fiber with a diameter of about 30 nm.
• If this chromatin is subjected to treatments that cause it to unfold
partially, it can be seen under the electron microscope as a series of
"beads on a string”.
• The string is DNA, and each bead is a "nucleosome core particle"
that consists of DNA wound around a protein core formed from
histones.
• The beads on a string represent the first level of chromosomal DNA
packing.
10. Nucleosomes as seen in the electron microscope.
(A) Chromatin isolated directly from an interphase nucleus appears in the electron
microscope as a thread 30 nm thick.
(B) (B) This electron micrograph shows a length of chromatin that has been experimentally
unpacked, or decondensed, after isolation to show the nucleosomes
11. • The structural organization of nucleosomes was
determined after first isolating them from unfolded
chromatin by digestion with particular enzymes (called
nucleases) that break down DNA by cutting between the
nucleosomes.
• After digestion for a short period, the exposed DNA
between the nucleosome core particles, the linker DNA, is
degraded.
• Each individual nucleosome core particle consists of a
– complex of eight histone proteins two molecules each of
histones H2A, H2B, H3, and H4
– and double-stranded DNA that is 146 nucleotide pairs long.
• The histone octamer forms a protein core around which the
double-stranded DNA is wound
12. Structural organization of the nucleosome. A
nucleosome
contains a protein core made of eight histone
molecules. As indicated, the
nucleosome core particle is released from
chromatin by digestion of the linker
DNA with a nuclease, an enzyme that breaks
down DNA. (The nuclease can degrade the
exposed linker DNA but cannot attack the DNA
wound tightly
around the nucleosome core.) After
dissociation of the isolated nucleosome
into its protein core and DNA, the length of the
DNA that was wound around
the core can be determined. This length of 146
nucleotide pairs is sufficient to
wrap 1.65 times around the histone core.
13. The structure of a nucleosome core particle, as determined by
x-ray diffraction analyses of crystals. Each histone is colored, with the DNA double
helix in light gray