This document provides an overview of the lactation process in dairy cows. It discusses how milk is produced in the mammary gland and secreted, the mechanism of milk letdown triggered by oxytocin release, and various milking procedures and factors that affect milk quantity and quality. The document also describes the pathway that a nerve impulse travels when a cow associates milking with the letdown reflex, causing oxytocin release from the pituitary gland and milk ejection from the alveoli through myoepithelial cell contraction. Stress can inhibit this process by releasing epinephrine instead of oxytocin.