SlideShare a Scribd company logo
Cellular antioxidant activity of rice bran oil
at different stages of refining
Tao Zhang, PhD Candidate
School of Food Science and Technology
State Key Laboratory of Food Science and Technology
International Joint Research Laboratory for Lipid Nutrition and Safety
May 23-25 |Hanoi, Vietnam
SCHOOL OF FOOD
SCIENCE AND TECHNOLOGYJIANGNAN UNIVERSITY
The 5th International Conference of Rice Bran Oil|2018
1
Outline
Structure of the presentation
Jiangnan University
 Introduction
 Cellular antioxidant activity (CAA) assay
 Results and discussions
 Summary and future perspectives
2
Introduction
Rice bran oil & unsaponifiable matter
Jiangnan University
0
10
20
30
40
50
Limitvalue(g/kgoil)
Limit value of unsaponifiable matter of
vegetable oil National Standard of China
Rice
Bioactive molecules
bran oil
Bioactive compound existed in RBO is in the form of unsaponifiable matter 3
Introduction
Minor components in rice bran oil
Jiangnan University
4
Introduction
Minor components in rice bran oil
Jiangnan University
Group A: Minor
components
removed (MCR)oil
Group B: Native
(N) oil
5
Introduction
Minor components in rice bran oil
Jiangnan University
Therefore, while studying the effect of rice bran oil on
antioxidant status in experimental systems, we should
consider the contributions of minor compounds present
in unsaponifiable fractions.
6
Introduction
Comparison of chemical and CAA assays
Jiangnan University
7
Introduction
Method and principle of CAA assay
Jiangnan University
Antiox = Methanol extract of oil 8
esterases
37 oC, 24 h
600 µM ABAP
37 oC, 1 h
Ex=485 nm
Em=538 nm
OME+ 25 µM
DCFA-DA
PBS
Wash
1 h
PBS
Wash
Introduction
Process flowchart of CAA assay
Jiangnan University
4×104/well
 
SA- BA
CAA unit 100 100
CA- BA
  
 
 
9
Samples
Detail information of rice bran oils
Jiangnan University
A1 A2 A3 A4 A5 A6 A7 A8
B1 B2 B3 B4 B5 B6 B7 B8 B9
C1 C2 C3 C4
Group A: Qinhuangdao Factory (8 samples)
Group B: Taizhou Factory (9 samples)
Group C: Ha’erbin Factory (4 samples)
Samples of Group A from Qinhuangdao Factory
A1 A2 A3 A4 A5 A6 A7 A8
10
Results
CAA assay model specification
Jiangnan University
Quercetin
Fluorescence was inhibited by quercetin in a dose-dependent manner. 11
Results
Cell viability of rice bran oil methanol extracts
Jiangnan University
Cell viability was higher than 90%, cytotoxicity was not observed.
Qinhuangdao factory
12
Results
Effect of refining process on CAA value
Jiangnan University
Qinhuangdao factory
Deacidification was a critical process that influenced the CAA value.
13
Effect of refining process on CAA value
Jiangnan University
Results
Why the CAA value changed significantly at the point of deacidification? 14
Effect of refining process on acid value
Jiangnan University
Results
The deacidification process efficiently removed the free fatty acids in the oil.
Qinhuangdao Factory Taizhou Factory Ha’erbin FactoryDeacidification
15
Effect of refining process on minor components
Jiangnan University
Results
The dewaxing process efficiently removed the minor components in the oil.
Deacidification Deacidification Deacidification
Deacidification Deacidification Deacidification
16
Effect of refining process on CAA
Jiangnan University
Results
Whether the changes of CAA value are really related to
free fatty acids and minor components?
 Key points during the refining process:
• Acid value (free fatty acids)
• Minor components.
• Deacidification
17
Effect of free fatty acid on CAA value
Jiangnan University
Results
Answer 1: Oil extracts with free fatty acids exhibited lower CAA value.
Oil extract
Oleic acid + extract
Control
18
Effect of methanol extract on CAA value
Jiangnan University
Results
Answer 2: Higher concentration of oil extracts exhibited higher CAA value.
Blank
Control
0.25 mg/mL
0.5 mg/mL
1.0 mg/mL
2.0 mg/mL
5.0 mg/mL
19
T-AOC SOD CAT
Control 0.66 ± 0.02 5.87 ± 0.24 12.66 ±1.50
H2O2 0.40 ± 0.01 1.79 ± 0.06 4.39 ± 0.20
Quercetin + H2O2 0.26±0.03 3.15±0.03 9.33±0.59
Crude oil + H2O2 0.23±0.00 4.15±0.10 7.48±0.37
Deacidification oil + H2O2 0.71±0.03 3.24±0.07 10.47±0.20
Refined oil product + H2O2 0.20±0.02 5.31±0.25 5.65±0.57
Effect of refining process on antioxidase
Jiangnan University
Results
 The rice bran oil with abundant minor components and less free
fatty acids exerted higher activity of antioxidase.
 Crude oil
• Higher free fatty acids
• Higher minor components
 Deacidification oil
• Higher minor components
• Less free fatty acid
 Refined oil
• Less free fatty acid
• Less minor components
20
Tocopherol Squalene Campesterol Stigmasterol Oryzanol Polyphenol
CAA value 0.644 0.585 0.909** 0.838** 0.725* 0.661
Tab. Pearson correlation coefficient among minor components and CAA value
Pearson correlation coefficient analysis
Jiangnan University
Results
(*p<0.05, **p<0.01)
 CAA value was significantly correlated with sterol and
oryzanol.
21
Proposed mechanisms
Jiangnan University
Results
Crude oil Extract
TAG FFA Minor components
ABAP
ROO·
DCFH-DA
DCFH
DA
DCF
ROO·
ROS·
ROS·
ROO·
ABAP
SOD, CAT↑
H2O
 Polarity: FFA > Minor components
a. Higher permeability rate of FFA
b. Minor components packaged by FFA
b
a
22
Edible oil needs precise and appropriate processing
Jiangnan University
Summary & Perspectives
 Attention should be given to minor constituents
• RBO contains sufficient amounts of endogenous minor constituents .
• Minor constituents have a vital role in providing anti-oxidant properties.
 Refining methods for oils should be optimized
• Edible oil needs precise and appropriate processing.
• Refining methods should be optimized to retain oryzanol and sterol.
23
The 5th International Conference of Rice Bran Oil|2015
This work was supported by “Science
Fund of Wilmar Global R&D Center”.
Acknowledgement
24

More Related Content

What's hot

Profile of High Stability Oils | AOCS 2015
Profile of High Stability Oils | AOCS 2015Profile of High Stability Oils | AOCS 2015
Profile of High Stability Oils | AOCS 2015
QUALISOY
 
1.1 int. class._of_edible_oil
1.1 int. class._of_edible_oil1.1 int. class._of_edible_oil
1.1 int. class._of_edible_oil
zayryn
 
edible oils and their quality aspects
edible oils and their quality aspectsedible oils and their quality aspects
edible oils and their quality aspects
Bhagya Vijayan
 
changes in edible oil quality
changes in edible oil qualitychanges in edible oil quality
changes in edible oil quality
sivsagar
 
#61M
#61M#61M
#61M
HardikT2
 
Manage Your Frying Oil
Manage Your Frying OilManage Your Frying Oil
Manage Your Frying Oil
Mark Moreno
 
Edible oil adulterations
Edible oil adulterationsEdible oil adulterations
Edible oil adulterations
Shubham Yadav
 
Ghee vs butter
Ghee vs butterGhee vs butter
Ghee vs butter
Pallavi Goswami
 
Aqueous extraction processing
Aqueous extraction processingAqueous extraction processing
Aqueous extraction processing
shaghaf kaukab
 
Fats and oils food production
Fats and oils food production Fats and oils food production
Fats and oils food production
Sanju Varghese
 
Palm kernel seed & oil
Palm kernel seed & oilPalm kernel seed & oil
Palm kernel seed & oil
Moksha Chib
 
Detection of oxidative status of some edible oil
Detection of oxidative status of some edible oilDetection of oxidative status of some edible oil
Detection of oxidative status of some edible oil
Seham Fawzy
 
Palm Oil, Palm Kernel Oil Process - Fractions, Derivatives and Product Uses
Palm Oil, Palm Kernel Oil Process - Fractions, Derivatives and Product UsesPalm Oil, Palm Kernel Oil Process - Fractions, Derivatives and Product Uses
Palm Oil, Palm Kernel Oil Process - Fractions, Derivatives and Product Uses
GreenPalm
 
#61
#61#61
Ghee boiling
Ghee boilingGhee boiling
Ghee boiling
Moksha Chib
 
Animal fats and oil
Animal fats and oilAnimal fats and oil
Animal fats and oil
Zuriati Zakaria
 
The Positive Attributes Required of Edible Oil Used in Deep Frying Applications
The Positive Attributes Required of Edible Oil Used in Deep Frying ApplicationsThe Positive Attributes Required of Edible Oil Used in Deep Frying Applications
The Positive Attributes Required of Edible Oil Used in Deep Frying Applications
Malaysian Palm Oil Council (MPOC Egypt)
 

What's hot (17)

Profile of High Stability Oils | AOCS 2015
Profile of High Stability Oils | AOCS 2015Profile of High Stability Oils | AOCS 2015
Profile of High Stability Oils | AOCS 2015
 
1.1 int. class._of_edible_oil
1.1 int. class._of_edible_oil1.1 int. class._of_edible_oil
1.1 int. class._of_edible_oil
 
edible oils and their quality aspects
edible oils and their quality aspectsedible oils and their quality aspects
edible oils and their quality aspects
 
changes in edible oil quality
changes in edible oil qualitychanges in edible oil quality
changes in edible oil quality
 
#61M
#61M#61M
#61M
 
Manage Your Frying Oil
Manage Your Frying OilManage Your Frying Oil
Manage Your Frying Oil
 
Edible oil adulterations
Edible oil adulterationsEdible oil adulterations
Edible oil adulterations
 
Ghee vs butter
Ghee vs butterGhee vs butter
Ghee vs butter
 
Aqueous extraction processing
Aqueous extraction processingAqueous extraction processing
Aqueous extraction processing
 
Fats and oils food production
Fats and oils food production Fats and oils food production
Fats and oils food production
 
Palm kernel seed & oil
Palm kernel seed & oilPalm kernel seed & oil
Palm kernel seed & oil
 
Detection of oxidative status of some edible oil
Detection of oxidative status of some edible oilDetection of oxidative status of some edible oil
Detection of oxidative status of some edible oil
 
Palm Oil, Palm Kernel Oil Process - Fractions, Derivatives and Product Uses
Palm Oil, Palm Kernel Oil Process - Fractions, Derivatives and Product UsesPalm Oil, Palm Kernel Oil Process - Fractions, Derivatives and Product Uses
Palm Oil, Palm Kernel Oil Process - Fractions, Derivatives and Product Uses
 
#61
#61#61
#61
 
Ghee boiling
Ghee boilingGhee boiling
Ghee boiling
 
Animal fats and oil
Animal fats and oilAnimal fats and oil
Animal fats and oil
 
The Positive Attributes Required of Edible Oil Used in Deep Frying Applications
The Positive Attributes Required of Edible Oil Used in Deep Frying ApplicationsThe Positive Attributes Required of Edible Oil Used in Deep Frying Applications
The Positive Attributes Required of Edible Oil Used in Deep Frying Applications
 

Similar to #61M 5th International Conference on Rice Bran Oil 24th - 25th May 2018, Hanoi, Vietnam

IRJET- Parameter Design for Optimum Percentage Yield for Bio-Diesel from ...
IRJET-  	  Parameter Design for Optimum Percentage Yield for Bio-Diesel from ...IRJET-  	  Parameter Design for Optimum Percentage Yield for Bio-Diesel from ...
IRJET- Parameter Design for Optimum Percentage Yield for Bio-Diesel from ...
IRJET Journal
 
Progress, prospect and challenges in glycerol purification process
Progress, prospect and challenges in glycerol purification processProgress, prospect and challenges in glycerol purification process
Progress, prospect and challenges in glycerol purification process
Bijaya Kumar Uprety
 
Oil to Biodiesel from Two Potential Sources – A 360 degree Comparative Study
Oil to Biodiesel from Two Potential Sources – A 360 degree Comparative StudyOil to Biodiesel from Two Potential Sources – A 360 degree Comparative Study
Oil to Biodiesel from Two Potential Sources – A 360 degree Comparative Study
Associate Professor in VSB Coimbatore
 
T 1 scope for biotechnology in paint industry - dr. d.k. bhattacharyya
T 1   scope for biotechnology in paint industry - dr. d.k. bhattacharyyaT 1   scope for biotechnology in paint industry - dr. d.k. bhattacharyya
T 1 scope for biotechnology in paint industry - dr. d.k. bhattacharyya
Gn Tewari
 
IRJET - Watermelon Seed Oil: Its Extraction, Analytical Studies, Modifica...
IRJET -  	  Watermelon Seed Oil: Its Extraction, Analytical Studies, Modifica...IRJET -  	  Watermelon Seed Oil: Its Extraction, Analytical Studies, Modifica...
IRJET - Watermelon Seed Oil: Its Extraction, Analytical Studies, Modifica...
IRJET Journal
 
IRJET- Process Optimization for Biodiesel Production from Sissoo Seed Oil
IRJET-  	  Process Optimization for Biodiesel Production from Sissoo Seed OilIRJET-  	  Process Optimization for Biodiesel Production from Sissoo Seed Oil
IRJET- Process Optimization for Biodiesel Production from Sissoo Seed Oil
IRJET Journal
 
An Evaluation of the Effect of Epoxidized Thevetia Nerrifolia Seed Oil Modifi...
An Evaluation of the Effect of Epoxidized Thevetia Nerrifolia Seed Oil Modifi...An Evaluation of the Effect of Epoxidized Thevetia Nerrifolia Seed Oil Modifi...
An Evaluation of the Effect of Epoxidized Thevetia Nerrifolia Seed Oil Modifi...
IOSR Journals
 
ELT10 Bangera - Free Fatty Acid Reduction in Edible Oils
ELT10 Bangera - Free Fatty Acid Reduction in Edible OilsELT10 Bangera - Free Fatty Acid Reduction in Edible Oils
ELT10 Bangera - Free Fatty Acid Reduction in Edible Oils
Rohit Bangera
 
IRJET-Performance and Emission Characteristics of C.I. Engine with Diesel-Bio...
IRJET-Performance and Emission Characteristics of C.I. Engine with Diesel-Bio...IRJET-Performance and Emission Characteristics of C.I. Engine with Diesel-Bio...
IRJET-Performance and Emission Characteristics of C.I. Engine with Diesel-Bio...
IRJET Journal
 
Food waste and food processing waste for renewable energy production
Food waste and food processing waste for renewable energy productionFood waste and food processing waste for renewable energy production
Food waste and food processing waste for renewable energy production
LPE Learning Center
 
Possibilities and Potentiality to increase-blending-ratio- of palm olein wit...
Possibilities and Potentiality to  increase-blending-ratio- of palm olein wit...Possibilities and Potentiality to  increase-blending-ratio- of palm olein wit...
Possibilities and Potentiality to increase-blending-ratio- of palm olein wit...
Adel Gabr Abdel-Razek
 
Cd33488492
Cd33488492Cd33488492
Cd33488492
IJERA Editor
 
Cd33488492
Cd33488492Cd33488492
Cd33488492
IJERA Editor
 
Pupal oil - An alternative feedstock for biodiesel production
 Pupal oil - An alternative feedstock for biodiesel production Pupal oil - An alternative feedstock for biodiesel production
Pupal oil - An alternative feedstock for biodiesel production
mithilasrim
 
Investigation of biodiesel from Canola oil deodorizer distillate using dual b...
Investigation of biodiesel from Canola oil deodorizer distillate using dual b...Investigation of biodiesel from Canola oil deodorizer distillate using dual b...
Investigation of biodiesel from Canola oil deodorizer distillate using dual b...
Associate Professor in VSB Coimbatore
 
J011646668
J011646668J011646668
J011646668
navdeep nandal
 
Effect of Extraction Method of Chia Seeds Oil on its Content of Fatty Acids a...
Effect of Extraction Method of Chia Seeds Oil on its Content of Fatty Acids a...Effect of Extraction Method of Chia Seeds Oil on its Content of Fatty Acids a...
Effect of Extraction Method of Chia Seeds Oil on its Content of Fatty Acids a...
IRJET Journal
 
Extraction of Gamma Oryzanol from Rice Bran Lecithin
Extraction of Gamma Oryzanol from Rice Bran LecithinExtraction of Gamma Oryzanol from Rice Bran Lecithin
Extraction of Gamma Oryzanol from Rice Bran Lecithin
IRJET Journal
 
#61N 5th International Conference on Rice Bran Oil 24th - 25th May 2018, Hano...
#61N 5th International Conference on Rice Bran Oil 24th - 25th May 2018, Hano...#61N 5th International Conference on Rice Bran Oil 24th - 25th May 2018, Hano...
#61N 5th International Conference on Rice Bran Oil 24th - 25th May 2018, Hano...
Solvent Extractors' Association of India
 
Benefits of Rice Bran Oil
Benefits of Rice Bran OilBenefits of Rice Bran Oil
Benefits of Rice Bran Oil
gloherbals
 

Similar to #61M 5th International Conference on Rice Bran Oil 24th - 25th May 2018, Hanoi, Vietnam (20)

IRJET- Parameter Design for Optimum Percentage Yield for Bio-Diesel from ...
IRJET-  	  Parameter Design for Optimum Percentage Yield for Bio-Diesel from ...IRJET-  	  Parameter Design for Optimum Percentage Yield for Bio-Diesel from ...
IRJET- Parameter Design for Optimum Percentage Yield for Bio-Diesel from ...
 
Progress, prospect and challenges in glycerol purification process
Progress, prospect and challenges in glycerol purification processProgress, prospect and challenges in glycerol purification process
Progress, prospect and challenges in glycerol purification process
 
Oil to Biodiesel from Two Potential Sources – A 360 degree Comparative Study
Oil to Biodiesel from Two Potential Sources – A 360 degree Comparative StudyOil to Biodiesel from Two Potential Sources – A 360 degree Comparative Study
Oil to Biodiesel from Two Potential Sources – A 360 degree Comparative Study
 
T 1 scope for biotechnology in paint industry - dr. d.k. bhattacharyya
T 1   scope for biotechnology in paint industry - dr. d.k. bhattacharyyaT 1   scope for biotechnology in paint industry - dr. d.k. bhattacharyya
T 1 scope for biotechnology in paint industry - dr. d.k. bhattacharyya
 
IRJET - Watermelon Seed Oil: Its Extraction, Analytical Studies, Modifica...
IRJET -  	  Watermelon Seed Oil: Its Extraction, Analytical Studies, Modifica...IRJET -  	  Watermelon Seed Oil: Its Extraction, Analytical Studies, Modifica...
IRJET - Watermelon Seed Oil: Its Extraction, Analytical Studies, Modifica...
 
IRJET- Process Optimization for Biodiesel Production from Sissoo Seed Oil
IRJET-  	  Process Optimization for Biodiesel Production from Sissoo Seed OilIRJET-  	  Process Optimization for Biodiesel Production from Sissoo Seed Oil
IRJET- Process Optimization for Biodiesel Production from Sissoo Seed Oil
 
An Evaluation of the Effect of Epoxidized Thevetia Nerrifolia Seed Oil Modifi...
An Evaluation of the Effect of Epoxidized Thevetia Nerrifolia Seed Oil Modifi...An Evaluation of the Effect of Epoxidized Thevetia Nerrifolia Seed Oil Modifi...
An Evaluation of the Effect of Epoxidized Thevetia Nerrifolia Seed Oil Modifi...
 
ELT10 Bangera - Free Fatty Acid Reduction in Edible Oils
ELT10 Bangera - Free Fatty Acid Reduction in Edible OilsELT10 Bangera - Free Fatty Acid Reduction in Edible Oils
ELT10 Bangera - Free Fatty Acid Reduction in Edible Oils
 
IRJET-Performance and Emission Characteristics of C.I. Engine with Diesel-Bio...
IRJET-Performance and Emission Characteristics of C.I. Engine with Diesel-Bio...IRJET-Performance and Emission Characteristics of C.I. Engine with Diesel-Bio...
IRJET-Performance and Emission Characteristics of C.I. Engine with Diesel-Bio...
 
Food waste and food processing waste for renewable energy production
Food waste and food processing waste for renewable energy productionFood waste and food processing waste for renewable energy production
Food waste and food processing waste for renewable energy production
 
Possibilities and Potentiality to increase-blending-ratio- of palm olein wit...
Possibilities and Potentiality to  increase-blending-ratio- of palm olein wit...Possibilities and Potentiality to  increase-blending-ratio- of palm olein wit...
Possibilities and Potentiality to increase-blending-ratio- of palm olein wit...
 
Cd33488492
Cd33488492Cd33488492
Cd33488492
 
Cd33488492
Cd33488492Cd33488492
Cd33488492
 
Pupal oil - An alternative feedstock for biodiesel production
 Pupal oil - An alternative feedstock for biodiesel production Pupal oil - An alternative feedstock for biodiesel production
Pupal oil - An alternative feedstock for biodiesel production
 
Investigation of biodiesel from Canola oil deodorizer distillate using dual b...
Investigation of biodiesel from Canola oil deodorizer distillate using dual b...Investigation of biodiesel from Canola oil deodorizer distillate using dual b...
Investigation of biodiesel from Canola oil deodorizer distillate using dual b...
 
J011646668
J011646668J011646668
J011646668
 
Effect of Extraction Method of Chia Seeds Oil on its Content of Fatty Acids a...
Effect of Extraction Method of Chia Seeds Oil on its Content of Fatty Acids a...Effect of Extraction Method of Chia Seeds Oil on its Content of Fatty Acids a...
Effect of Extraction Method of Chia Seeds Oil on its Content of Fatty Acids a...
 
Extraction of Gamma Oryzanol from Rice Bran Lecithin
Extraction of Gamma Oryzanol from Rice Bran LecithinExtraction of Gamma Oryzanol from Rice Bran Lecithin
Extraction of Gamma Oryzanol from Rice Bran Lecithin
 
#61N 5th International Conference on Rice Bran Oil 24th - 25th May 2018, Hano...
#61N 5th International Conference on Rice Bran Oil 24th - 25th May 2018, Hano...#61N 5th International Conference on Rice Bran Oil 24th - 25th May 2018, Hano...
#61N 5th International Conference on Rice Bran Oil 24th - 25th May 2018, Hano...
 
Benefits of Rice Bran Oil
Benefits of Rice Bran OilBenefits of Rice Bran Oil
Benefits of Rice Bran Oil
 

More from Solvent Extractors' Association of India

Mr. sandeep bhan palmoil
Mr. sandeep bhan palmoilMr. sandeep bhan palmoil
Mr. nagaraj meda transgraph
Mr. nagaraj meda   transgraphMr. nagaraj meda   transgraph
Mr. nagaraj meda transgraph
Solvent Extractors' Association of India
 
Dr180824
Dr180824Dr180824
Doc2
Doc2Doc2
Dr180817
Dr180817Dr180817
Finaly ec 112
Finaly ec 112Finaly ec 112
Progress of sowing of kharif crops 2018-19 -website - 9.8.18
Progress of sowing of kharif  crops   2018-19 -website - 9.8.18Progress of sowing of kharif  crops   2018-19 -website - 9.8.18
Progress of sowing of kharif crops 2018-19 -website - 9.8.18
Solvent Extractors' Association of India
 
Dr180810
Dr180810Dr180810
Export of oilmeals july
Export of oilmeals julyExport of oilmeals july
450
450450
#245 Tariff Structure as on 15th June 2018
#245 Tariff Structure as on 15th June 2018#245 Tariff Structure as on 15th June 2018
#245 Tariff Structure as on 15th June 2018
Solvent Extractors' Association of India
 
Export of castor oil 2017 18-website
Export of castor oil  2017 18-websiteExport of castor oil  2017 18-website
Export of castor oil 2017 18-website
Solvent Extractors' Association of India
 
1229
12291229
Itc
ItcItc
172
172172
172
172172
1174
11741174
398
398398
40
4040

More from Solvent Extractors' Association of India (20)

Mr. sandeep bhan palmoil
Mr. sandeep bhan palmoilMr. sandeep bhan palmoil
Mr. sandeep bhan palmoil
 
Mr. nagaraj meda transgraph
Mr. nagaraj meda   transgraphMr. nagaraj meda   transgraph
Mr. nagaraj meda transgraph
 
Imp veg 13 to 14
Imp veg 13 to 14Imp veg 13 to 14
Imp veg 13 to 14
 
Dr180824
Dr180824Dr180824
Dr180824
 
Doc2
Doc2Doc2
Doc2
 
Dr180817
Dr180817Dr180817
Dr180817
 
Finaly ec 112
Finaly ec 112Finaly ec 112
Finaly ec 112
 
Progress of sowing of kharif crops 2018-19 -website - 9.8.18
Progress of sowing of kharif  crops   2018-19 -website - 9.8.18Progress of sowing of kharif  crops   2018-19 -website - 9.8.18
Progress of sowing of kharif crops 2018-19 -website - 9.8.18
 
Dr180810
Dr180810Dr180810
Dr180810
 
Export of oilmeals july
Export of oilmeals julyExport of oilmeals july
Export of oilmeals july
 
450
450450
450
 
#245 Tariff Structure as on 15th June 2018
#245 Tariff Structure as on 15th June 2018#245 Tariff Structure as on 15th June 2018
#245 Tariff Structure as on 15th June 2018
 
Export of castor oil 2017 18-website
Export of castor oil  2017 18-websiteExport of castor oil  2017 18-website
Export of castor oil 2017 18-website
 
1229
12291229
1229
 
Itc
ItcItc
Itc
 
172
172172
172
 
172
172172
172
 
1174
11741174
1174
 
398
398398
398
 
40
4040
40
 

#61M 5th International Conference on Rice Bran Oil 24th - 25th May 2018, Hanoi, Vietnam

  • 1. Cellular antioxidant activity of rice bran oil at different stages of refining Tao Zhang, PhD Candidate School of Food Science and Technology State Key Laboratory of Food Science and Technology International Joint Research Laboratory for Lipid Nutrition and Safety May 23-25 |Hanoi, Vietnam SCHOOL OF FOOD SCIENCE AND TECHNOLOGYJIANGNAN UNIVERSITY The 5th International Conference of Rice Bran Oil|2018 1
  • 2. Outline Structure of the presentation Jiangnan University  Introduction  Cellular antioxidant activity (CAA) assay  Results and discussions  Summary and future perspectives 2
  • 3. Introduction Rice bran oil & unsaponifiable matter Jiangnan University 0 10 20 30 40 50 Limitvalue(g/kgoil) Limit value of unsaponifiable matter of vegetable oil National Standard of China Rice Bioactive molecules bran oil Bioactive compound existed in RBO is in the form of unsaponifiable matter 3
  • 4. Introduction Minor components in rice bran oil Jiangnan University 4
  • 5. Introduction Minor components in rice bran oil Jiangnan University Group A: Minor components removed (MCR)oil Group B: Native (N) oil 5
  • 6. Introduction Minor components in rice bran oil Jiangnan University Therefore, while studying the effect of rice bran oil on antioxidant status in experimental systems, we should consider the contributions of minor compounds present in unsaponifiable fractions. 6
  • 7. Introduction Comparison of chemical and CAA assays Jiangnan University 7
  • 8. Introduction Method and principle of CAA assay Jiangnan University Antiox = Methanol extract of oil 8 esterases
  • 9. 37 oC, 24 h 600 µM ABAP 37 oC, 1 h Ex=485 nm Em=538 nm OME+ 25 µM DCFA-DA PBS Wash 1 h PBS Wash Introduction Process flowchart of CAA assay Jiangnan University 4×104/well   SA- BA CAA unit 100 100 CA- BA        9
  • 10. Samples Detail information of rice bran oils Jiangnan University A1 A2 A3 A4 A5 A6 A7 A8 B1 B2 B3 B4 B5 B6 B7 B8 B9 C1 C2 C3 C4 Group A: Qinhuangdao Factory (8 samples) Group B: Taizhou Factory (9 samples) Group C: Ha’erbin Factory (4 samples) Samples of Group A from Qinhuangdao Factory A1 A2 A3 A4 A5 A6 A7 A8 10
  • 11. Results CAA assay model specification Jiangnan University Quercetin Fluorescence was inhibited by quercetin in a dose-dependent manner. 11
  • 12. Results Cell viability of rice bran oil methanol extracts Jiangnan University Cell viability was higher than 90%, cytotoxicity was not observed. Qinhuangdao factory 12
  • 13. Results Effect of refining process on CAA value Jiangnan University Qinhuangdao factory Deacidification was a critical process that influenced the CAA value. 13
  • 14. Effect of refining process on CAA value Jiangnan University Results Why the CAA value changed significantly at the point of deacidification? 14
  • 15. Effect of refining process on acid value Jiangnan University Results The deacidification process efficiently removed the free fatty acids in the oil. Qinhuangdao Factory Taizhou Factory Ha’erbin FactoryDeacidification 15
  • 16. Effect of refining process on minor components Jiangnan University Results The dewaxing process efficiently removed the minor components in the oil. Deacidification Deacidification Deacidification Deacidification Deacidification Deacidification 16
  • 17. Effect of refining process on CAA Jiangnan University Results Whether the changes of CAA value are really related to free fatty acids and minor components?  Key points during the refining process: • Acid value (free fatty acids) • Minor components. • Deacidification 17
  • 18. Effect of free fatty acid on CAA value Jiangnan University Results Answer 1: Oil extracts with free fatty acids exhibited lower CAA value. Oil extract Oleic acid + extract Control 18
  • 19. Effect of methanol extract on CAA value Jiangnan University Results Answer 2: Higher concentration of oil extracts exhibited higher CAA value. Blank Control 0.25 mg/mL 0.5 mg/mL 1.0 mg/mL 2.0 mg/mL 5.0 mg/mL 19
  • 20. T-AOC SOD CAT Control 0.66 ± 0.02 5.87 ± 0.24 12.66 ±1.50 H2O2 0.40 ± 0.01 1.79 ± 0.06 4.39 ± 0.20 Quercetin + H2O2 0.26±0.03 3.15±0.03 9.33±0.59 Crude oil + H2O2 0.23±0.00 4.15±0.10 7.48±0.37 Deacidification oil + H2O2 0.71±0.03 3.24±0.07 10.47±0.20 Refined oil product + H2O2 0.20±0.02 5.31±0.25 5.65±0.57 Effect of refining process on antioxidase Jiangnan University Results  The rice bran oil with abundant minor components and less free fatty acids exerted higher activity of antioxidase.  Crude oil • Higher free fatty acids • Higher minor components  Deacidification oil • Higher minor components • Less free fatty acid  Refined oil • Less free fatty acid • Less minor components 20
  • 21. Tocopherol Squalene Campesterol Stigmasterol Oryzanol Polyphenol CAA value 0.644 0.585 0.909** 0.838** 0.725* 0.661 Tab. Pearson correlation coefficient among minor components and CAA value Pearson correlation coefficient analysis Jiangnan University Results (*p<0.05, **p<0.01)  CAA value was significantly correlated with sterol and oryzanol. 21
  • 22. Proposed mechanisms Jiangnan University Results Crude oil Extract TAG FFA Minor components ABAP ROO· DCFH-DA DCFH DA DCF ROO· ROS· ROS· ROO· ABAP SOD, CAT↑ H2O  Polarity: FFA > Minor components a. Higher permeability rate of FFA b. Minor components packaged by FFA b a 22
  • 23. Edible oil needs precise and appropriate processing Jiangnan University Summary & Perspectives  Attention should be given to minor constituents • RBO contains sufficient amounts of endogenous minor constituents . • Minor constituents have a vital role in providing anti-oxidant properties.  Refining methods for oils should be optimized • Edible oil needs precise and appropriate processing. • Refining methods should be optimized to retain oryzanol and sterol. 23
  • 24. The 5th International Conference of Rice Bran Oil|2015 This work was supported by “Science Fund of Wilmar Global R&D Center”. Acknowledgement 24