This document discusses the design of PCR primers for a DNA template. A forward primer and reverse primer were designed that are complementary to the 5' and 3' ends of the template respectively. The forward primer is 5'-AGACTCAGAGAGAACCC-3' and the reverse primer is 5'-ATTCAAAGACCACGGGG-3'.

1. 1
Design of PCR primers
Template
3’-TCTGAGTCTCTCTTGGG------GGGGCACCAGAAACTTA-5’
5’-AGACTCAGAGAGAACCC------CCCCGTGGTCTTTGAAT-3’
Forward primer –
5’-AGACTCAGAGAGAACCC-3’
Reverse primer –
5’-ATTCAAAGACCACGGGG-3’