This document summarizes information about operations and services at the CCRMN newcomers meeting. It outlines how to register as a user, submit analysis requests, prepare samples, access spectrometers and data, and opportunities for training on instruments and collaboration. Key points covered are how to register as a user, submit analysis requests online, requirements for sample preparation like tube height, and availability of on-demand and self-service analyses after training.

1. CCRMN newcomers meeting

2. Fonctionnement du service
 Check the web site : https://ccrmn.univ-lyon1.fr/

3. CCRMN general operation
 Please register yourself !

4.  Please register yourself ! https://ccrmn-request.univ-lyon1.fr/
CCRMN general operation

5.  Please register yourself !
CCRMN general operation

6. - We set your rights as « user » on GRR
spectrometers planning
- We set your rights to access your spectra
and send the procedure to connect our
network drive
- You are ready to send us analyses requests
via https://ccrmn-request.univ-lyon1.fr/
 Please register yourself !
CCRMN general operation

7.  Analyses upon request : ccrmn-request.univ-lyon1.fr
CCRMN general operation
- Special needs / parameters shoud be entered in the title
- Experiments/ solvants can be added/modified if needed
- PLEASE no space or special characters in the file names

8.  No broken tubes on the
sample changers
CCRMN general operation
 Please get your tubes back !

9. pay attention to the solvent height in your tube : minimum 3,7mm
CCRMN general operation

10. Solubilité Préparation
CCRMN general operation

11.  Special tubes : Maximum height 22 cm for 500 or AVL300 sample changers
CCRMN general operation

12. • Spectra processing : Tospin, Mnova, ACD
• DOSY processing : Mnova, Dynamic Center
• Spectra simulation : ACD, Mnova
• Automatic interprétation : Mnova, CMC assist, ACD
 Workstation available
CCRMN general operation

13.  Chemistry lab available
CCRMN general operation

14. • Raw data on university network drive
• At least 3 last months data available
• Archiving at least once a year
• Archives by year available on request since 2004
 Data archiving and retrieval
CCRMN general operation

15. CCRMN general operation
• On demand analyses
• Self-service analyzes after training
• New experiments set-up
• Collaborations

16. CCRMN general operation
• On demand analyses
• Self-service analyzes after training
• New experiments set-up / collaborations

17. CCRMN general operation
• On demand analyses :
Go to https://ccrmn-request.univ-lyon1.fr
Bring your tube properly prepared
Recover your spectrum on the network drive
Get your tube back
Available on all spectrometers
2 probes on the 500 MHz :
longer delays
- BBI : ok for 1H on very dilute
solutions, 2D, DOSY, 13C{19F}
- BBFO (being repaired) : OK for
non sensitive « X » nuclei : 13C,
29SI, 15N … 19F{1H]

18. spectro 300 400 500
sensibilité 13
C 110 190 290
Prix /heure 6,6 7,7 8,8
Prix/tube
13
C 5mg
105,6 35,2 8,8
Prix/tube
13
C 10mg
13,2 7,7 4,4
Prix/tube
13
C 100mg
1,7 1,9 2,2
• Give us some product !!!
• Tell us what you are looking for
• Beware of spectrometer choice
• Let us know compound mass in the tube,
or MW, or NS
• Ask for an appointment for analyzes on
sensitive products
• Tell us if you need quantitative analyses
(by default they are not !)
• On demand analyses :
CCRMN general operation

19. • On demand analyses
• Self-service analyzes after training
• New experiments set-up
• Collaborations
CCRMN general operation
1. Train with training with an experienced
user of your research group
2. Validation with CCRMN staff : ask for an
appointment ccrmn@univ-lyon1.fr

20. CCRMN general operation
Sample changer queue visible remotely, please contact
us to get a login/password
Self-service analyzes after training : AVL 300
Continuous sample changer - Accessible any time to authorized persons
> 10mn expts done after 7 pm

21. CCRMN general operation
On line planning, 2 *10mn slots / person
Please contact us for special needs (kinetics …)
Self-service analyzes after training : AV 300 - Manual operation

22. CCRMN general operation
• On demand analyses
• Self-service analyzes after training
• New experiments set-up / collaborations

23. 1H {1H}
Proton double presat
INEPT 89Y et 29Si
Combined experiments (HSCQ-Tocsy, HMBC-Noesy …),
Quantitative 13C et 29Si
Variable temperature down to -100°C on the 500 MHz
 New experiments setup (all spectrometers)
CCRMN general operation

24.  Quality approach : volunteers wanted!
CCRMN general operation

25. Zoom about quantitative analysis
Conditions :
• Non saturated receiver
• Non troncated spectrum
• Correct Phase / intégration
• Long enough relaxation time

26. Zoom about quantitative analysis
 Non saturated receiver

27. Zoom about quantitative analysis
 Non troncated spectrum

28. Zoom about quantitative analysis
 Correct phase / intégration

29. Zoom about quantitative analysis
 Long enough relaxation time
90x
T1 Relaxation :
Back to thermal equilibrium
Recover population excess on z axis
t
90x
D1 D1
90x
*NS
T1 5*T1
Mz = M0(1- exp-t/T1)
Mz
M0
M0(1- 1/e) ≈ 0,37
M0(1- e-5) ≈ 0,99
z z

30. Thanks for your attention
ccrmn@univ-lyon1.fr