This study isolated and characterized mutants of Agrobacterium tumefaciens that were unable to utilize octopine, octopinic acid, and lysopine as nitrogen sources. Of the 55 mutant strains isolated, 11 mutants were solely altered in their ability to utilize these compounds, while retaining the ability to utilize other amino acids. These 11 mutants showed a high correlation between inability to utilize the compounds and loss of virulence. All mutants contained the Ti plasmid. Virulent mutants actively transported octopine when pre-grown in its presence, while avirulent mutants did not, and only virulent mutants induced tumors containing octopine. The results relate to the hypothesis that bacterial genes code for the octopine-
Van Houten, 2009, Desulfovibrio Paquesii Sp. Nov., A Hydrogenotrophic Sulfate...roelmeulepas
This document describes the isolation and characterization of a novel sulfate-reducing bacterium, strain SB1T, isolated from a synthesis-gas-fed bioreactor treating zinc- and sulfate-rich wastewater. 16S rRNA gene sequencing showed that strain SB1T was most closely related to Desulfovibrio gigas, with 97.5% sequence similarity. However, DNA-DNA hybridization showed only 56% relatedness between the two strains, below the threshold for species designation. Phenotypic characterization also differentiated strain SB1T from D. gigas. Therefore, a new species, Desulfovibrio paquesii sp. nov., is proposed to accommodate strain SB1T.
2019 - Profiling of filamentous bacteria in activated sludge by 16s RNA ampli...WALEBUBLÉ
Abstract: In this study, filamentous bacteria in the activated sludge of a WWTP were investigated throughout a one-year period using high-throughput short-read (Illumina) and full-length (PacBio) 16S rRNA gene amplicon sequencing. The results showed that a total of 28 filamentous bacteria genera
were identified using Illumina sequencing. Also, we found 25 species using PacBio sequencing, belonging to Curvibacter, Mycobacterium, Haliscomenobacter, Defluvicoccus, Sphaerotilus, Thiothrix, Leptothrix, Gordonia and Tetrasphaera genera. Active Volatile Suspended Solids (AVSS) were
calculated from ATP data contained in living microorganisms, this parameter represents the living biomass concentration, and the food/microorganisms ratio (F/M ratio) was calculated using AVSS instead of MLVSS. To assess the contribution of the F/M ratio to the variability observed in the filamentous bacteria structure we carried out distance-based linear models (DISTLM) and distancebased redundancy analysis (dbRDA).
2008 - Molecular microbial and chemical investigation of the bioremediation o...WALEBUBLÉ
The document describes a study that used laboratory-scale bioreactors to investigate the biodegradation of two-phase olive mill waste (TPOMW) by its indigenous microbiota under different conditions. The effects of nutrient addition and aeration on bioremediation and microbial community changes were evaluated. Analysis found that nutrient addition and aeration led to greater decreases in polyphenolic content and increases in the fungal to bacterial ratio. Molecular identification of bacteria and fungi in the bioreactors identified several genera present, with fungi like Penicillium and Candida dominant.
2017 - Effect of ozone addition to control Gordonia foaming on the nitrifying...WALEBUBLÉ
The ozonation of activated sludge has been used as a technical measure for bulking control in a high number of full-scale wastewater treatment plants (WWTP), despite a lack of precise
predictions on the level of reduction in filament growth or the lack of knowledge of impact on microbial community from this technique. Ozone is a strong oxidant reacting rapidly with
suspended solids. Various studies have suggested that ozone attacks the bacterial cell surface, alters the permeability of the cell membrane and ultimately results in the leakage of cell
contents. However, the microbes in the sludge form a complex matrix, and ozone may affect bacterial populations at different rates different depending on their locations in the floc or their
capacity for adaptation. Nitrification, a key step of the nitrogen cycle, is the sequential oxidation of ammonia via nitrite to nitrate. This process is catalysed by ammonia-oxidizing bacteria
(AOB) and nitrite-oxidizing bacteria (NOB), whose cooperation is needed to achieve complete nitrification. Although the nitrification process in WWTPs has been investigated in depth, the response of microbial communities are still a focus of considerable interest due to their high sensitivity to inhibitory compounds and environmental factors that results in repeated
breakdowns of nitrification performance. In this study, we focus on two aspects that have not been thoroughly considered in previous studies; the use of ozone for Gordonia foaming
elimination on dynamic population of a nitrifying bacterial community, and the nitrification performance of activated sludge system.
This study analyzed the occurrence and diversity of integrons in bacteria isolated from an urban wastewater treatment plant. A total of 697 isolates of Enterobacteriaceae and Aeromonas were screened for integrons. Three new gene cassettes were identified, including a novel aadA variant and genes involved in cell signaling and unknown functions. Thirteen different gene cassette arrays were detected, with four representing novel integrons. Approximately 80% of isolates were resistant to at least 3 antibiotic classes. The presence of novel integron structures in treated effluent suggests wastewater treatment plants may facilitate the formation and spread of antibiotic resistance genes.
This document summarizes research on natural clays that exhibit antibacterial properties. Key findings include:
- Certain clays from hydrothermally altered volcanic environments can eliminate a wide range of bacteria, including antibiotic-resistant strains.
- The most effective antibacterial clay studied comes from an open pit mine in the Cascade Mountains and contains nanoscale illite-smectite and reduced iron phases.
- When hydrated, this clay releases soluble constituents like iron that are toxic to bacteria but below published minimum inhibitory concentrations. The clay appears to buffer pH and oxidation states in a way that promotes soluble iron release.
This study isolated and characterized mutants of Agrobacterium tumefaciens that were unable to utilize octopine, octopinic acid, and lysopine as nitrogen sources. Of the 55 mutant strains isolated, 11 mutants were solely altered in their ability to utilize these compounds, while retaining the ability to utilize other amino acids. These 11 mutants showed a high correlation between inability to utilize the compounds and loss of virulence. All mutants contained the Ti plasmid. Virulent mutants actively transported octopine when pre-grown in its presence, while avirulent mutants did not, and only virulent mutants induced tumors containing octopine. The results relate to the hypothesis that bacterial genes code for the octopine-
Van Houten, 2009, Desulfovibrio Paquesii Sp. Nov., A Hydrogenotrophic Sulfate...roelmeulepas
This document describes the isolation and characterization of a novel sulfate-reducing bacterium, strain SB1T, isolated from a synthesis-gas-fed bioreactor treating zinc- and sulfate-rich wastewater. 16S rRNA gene sequencing showed that strain SB1T was most closely related to Desulfovibrio gigas, with 97.5% sequence similarity. However, DNA-DNA hybridization showed only 56% relatedness between the two strains, below the threshold for species designation. Phenotypic characterization also differentiated strain SB1T from D. gigas. Therefore, a new species, Desulfovibrio paquesii sp. nov., is proposed to accommodate strain SB1T.
2019 - Profiling of filamentous bacteria in activated sludge by 16s RNA ampli...WALEBUBLÉ
Abstract: In this study, filamentous bacteria in the activated sludge of a WWTP were investigated throughout a one-year period using high-throughput short-read (Illumina) and full-length (PacBio) 16S rRNA gene amplicon sequencing. The results showed that a total of 28 filamentous bacteria genera
were identified using Illumina sequencing. Also, we found 25 species using PacBio sequencing, belonging to Curvibacter, Mycobacterium, Haliscomenobacter, Defluvicoccus, Sphaerotilus, Thiothrix, Leptothrix, Gordonia and Tetrasphaera genera. Active Volatile Suspended Solids (AVSS) were
calculated from ATP data contained in living microorganisms, this parameter represents the living biomass concentration, and the food/microorganisms ratio (F/M ratio) was calculated using AVSS instead of MLVSS. To assess the contribution of the F/M ratio to the variability observed in the filamentous bacteria structure we carried out distance-based linear models (DISTLM) and distancebased redundancy analysis (dbRDA).
2008 - Molecular microbial and chemical investigation of the bioremediation o...WALEBUBLÉ
The document describes a study that used laboratory-scale bioreactors to investigate the biodegradation of two-phase olive mill waste (TPOMW) by its indigenous microbiota under different conditions. The effects of nutrient addition and aeration on bioremediation and microbial community changes were evaluated. Analysis found that nutrient addition and aeration led to greater decreases in polyphenolic content and increases in the fungal to bacterial ratio. Molecular identification of bacteria and fungi in the bioreactors identified several genera present, with fungi like Penicillium and Candida dominant.
2017 - Effect of ozone addition to control Gordonia foaming on the nitrifying...WALEBUBLÉ
The ozonation of activated sludge has been used as a technical measure for bulking control in a high number of full-scale wastewater treatment plants (WWTP), despite a lack of precise
predictions on the level of reduction in filament growth or the lack of knowledge of impact on microbial community from this technique. Ozone is a strong oxidant reacting rapidly with
suspended solids. Various studies have suggested that ozone attacks the bacterial cell surface, alters the permeability of the cell membrane and ultimately results in the leakage of cell
contents. However, the microbes in the sludge form a complex matrix, and ozone may affect bacterial populations at different rates different depending on their locations in the floc or their
capacity for adaptation. Nitrification, a key step of the nitrogen cycle, is the sequential oxidation of ammonia via nitrite to nitrate. This process is catalysed by ammonia-oxidizing bacteria
(AOB) and nitrite-oxidizing bacteria (NOB), whose cooperation is needed to achieve complete nitrification. Although the nitrification process in WWTPs has been investigated in depth, the response of microbial communities are still a focus of considerable interest due to their high sensitivity to inhibitory compounds and environmental factors that results in repeated
breakdowns of nitrification performance. In this study, we focus on two aspects that have not been thoroughly considered in previous studies; the use of ozone for Gordonia foaming
elimination on dynamic population of a nitrifying bacterial community, and the nitrification performance of activated sludge system.
This study analyzed the occurrence and diversity of integrons in bacteria isolated from an urban wastewater treatment plant. A total of 697 isolates of Enterobacteriaceae and Aeromonas were screened for integrons. Three new gene cassettes were identified, including a novel aadA variant and genes involved in cell signaling and unknown functions. Thirteen different gene cassette arrays were detected, with four representing novel integrons. Approximately 80% of isolates were resistant to at least 3 antibiotic classes. The presence of novel integron structures in treated effluent suggests wastewater treatment plants may facilitate the formation and spread of antibiotic resistance genes.
This document summarizes research on natural clays that exhibit antibacterial properties. Key findings include:
- Certain clays from hydrothermally altered volcanic environments can eliminate a wide range of bacteria, including antibiotic-resistant strains.
- The most effective antibacterial clay studied comes from an open pit mine in the Cascade Mountains and contains nanoscale illite-smectite and reduced iron phases.
- When hydrated, this clay releases soluble constituents like iron that are toxic to bacteria but below published minimum inhibitory concentrations. The clay appears to buffer pH and oxidation states in a way that promotes soluble iron release.
2017 - Comparison of nitrifying microbial communities of two full-scale membr...WALEBUBLÉ
Barbarroja, P., Moreno-Mesonero, L., Zornoza, A., Fernández-Navarro, J., Alonso, J.L., Muñagorri, F., García, C., Álvarez, C. (2017) Comparison of nitrifying microbial communities of two full-scale membrane bioreactors treating wastewaters from municipal solid wastes using 16S rDNA gene amplicon sequencing. 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
2009,planta, nitric oxide ros chitosan, srivastava et alNupur Srivastava
1) The study found that nitric oxide (NO) production occurs downstream of reactive oxygen species (ROS) in guard cells during chitosan-induced stomatal closure in pea plants.
2) Using fluorescent probes, the study showed that chitosan exposure led to increased ROS levels within 5 minutes, while NO levels rose after 10 minutes. Inhibiting ROS prevented the rise in both ROS and NO, but inhibiting NO only prevented the rise in NO.
3) The results suggest that chitosan signaling leads to ROS production, which then triggers NO production, and both ROS and NO are required for chitosan-induced stomatal closure.
V43n4 microbiological quality of salmon (salmo salar) sold in cities of the s...Natália Nespolo
This study analyzed the microbiological quality of 31 salmon samples sold in retail markets in São Paulo, Brazil. Results found:
- Mesophilic bacteria populations ranged from 10 to 3.9 x 106 CFU/g, with frozen samples having significantly lower levels.
- Total coliforms were found in 32.25% of samples at levels up to 1.1 x 103 MPN/g. Thermotolerant coliforms were found in 19.35% of samples up to 4.6 x 102 MPN/g.
- Aeromonas sp. was isolated in 41.95% of samples ranging from 2.0 x 102 to 8.0 x 103 CFU/g. Stap
2010 - A new species of genus Metacystis from a Wastewater Treatment PlantWALEBUBLÉ
ABSTRACT. Unusual prostomatid specimens were found in the biological reactor of a wastewater treatment plant in a health resort in Valencia, Spain. These ciliates were attached to flocs unlike other free-swimming prostomatid ciliates described to date in the mixed liquor of activated sludge plants. The morphological study of this species led to a typically different combination of characteristics: elongated
cell shape, 20–30 somatic kineties, 2 perioral kineties, and 1 circumoral kinety, 1 large vacuole protruding at the terminal end, a lorica tapered toward the aperture with a smooth neck, and 11–16 annular ridges. These characteristics place this representative as a new species of the genus Metacystis—Metacystis galiani n. sp. This species became the dominant population within the biological reactor when high values of conductivity (4,244 mS/cm) and temperature (26.8 1C) were recorded.
This document outlines a study investigating the effects of statin drugs like Simvastatin on the gut microbiome in relation to clinical obesity. The hypothesis is that certain statins will selectively inhibit bacteria in the Fimicutes phylum, which are more prevalent in obese individuals. The methods test the susceptibility of various bacterial species to different statins in vitro. Results show the statins had varying effects, with some inhibiting Clostridium species. Future experiments will examine changes in fecal microbiome DNA from patients taking Simvastatin.
Cellular damage to phytophthora infestans in tomato leaves treated with oxadi...dinomasch
The document describes an ultrastructural study of cells of Phytophthora infestans, the pathogen that causes late blight in tomatoes and potatoes, after treatment with the fungicide oxadixyl. The study found that in some hyphae and haustoria of P. infestans in infected tomato leaves treated with oxadixyl, there was massive accumulation of electron-dense deposits near the plasma membrane and mitochondria. This led to mitochondrial degeneration and likely cell death. However, no changes were observed in the endoplasmic reticulum or number of ribosomes. Some hyphal cell walls were also thickened after oxadixyl treatment. The damage to fungal cells from oxadix
The document summarizes a study conducted by a biology student that found antibiotic-resistant bacteria present in higher numbers in February compared to October at a local daycare center in North Carolina. Swabs were taken from various surfaces in a toddler room in the fall and winter. Five bacterial species showing resistance to multiple antibiotics were identified in October, compared to fourteen species in February. The student used microscopy, biochemical testing, and DNA sequencing to identify the bacteria isolated. The results indicate antibiotic-resistant bacteria pose a legitimate health threat in daycare centers.
1.paola 5.anthony hernandez phages report pdf correctedpcaballero21
This study isolated and characterized a novel mycobacteriophage (bacterial virus) named Incognito from soil in Puerto Rico. Incognito was isolated from soil collected under a plantain tree and purified through plaque purification. Electron microscopy and SDS-PAGE gel electrophoresis were used to characterize the phage's morphology and protein profile. Incognito produced turbid plaques and had a concentration of 3 x 107 plaque forming units per mL. Its protein profile showed similarities to other mycobacteriophages. This study demonstrates that Puerto Rico's tropical environment supports diverse phage populations with potential applications in phage therapy.
The document summarizes research into what makes certain natural clays antibacterial. Key points:
- Antibacterial clays contain nano-scale illite-smectite and reduced iron phases that buffer water pH and oxidation state, promoting Fe2+ solubility.
- E. coli exposed to an antibacterial clay's leachate accumulate high intracellular concentrations of Fe and P, supporting a role for polyphosphate or phospholipids in regulating Fe2+.
- Excess Fe2+ overwhelms cell membrane regulatory proteins, then oxidizes inside cells to Fe3+, producing lethal hydroxyl radicals through the Fenton reaction.
- High-resolution SEM images show the nano-scale crystals and Fe
This study characterized esterases associated with profenofos resistance in the tobacco budworm, Heliothis virescens. The researchers found that esterase (EST) activities were significantly higher in profenofos-resistant larvae compared to susceptible larvae, and EST activities highly correlated with resistance to profenofos. Electrophoretic gels revealed both quantitative and qualitative differences in EST bands between resistant and susceptible larvae. A band designated A' was expressed only in resistant larvae and increased in frequency after selection with profenofos. Reduction in acetylcholinesterase sensitivity to inhibition was also observed in resistant larvae. These results suggest EST assays can detect and monitor profenofos resistance in H. v
Pesticidas em processos fermentativos do vinhoLaura Mascarin
The presence of six fungicides (azoxystrobin, cyprodinil, fludioxonil, mepanipyrim, pyrimethanil, and tetraconazole) did not negatively affect alcoholic fermentation by two yeasts (Saccharomyces cerevisiae and Kloeckera apiculata), and in some cases stimulated higher alcohol production. During malolactic fermentation by two lactic acid bacteria (Leuconostoc oenos and Lactobacillus plantarum), malic acid degradation was slightly lower in the presence of pesticides, except for mepanipyrim which had little effect. The fermentative microorganisms did not degrade or remove the
Response of aquatic fern(Azolla), to watercontaminationKavitha Cingam
The document summarizes a study on the response of the aquatic fern Azolla to contamination by the antibiotic ciprofloxacin (Cipro). The study investigated Azolla's ability to uptake Cipro and the effects on its nitrogen-fixing process. The results showed that Azolla is able to uptake concentrations of Cipro above levels that classify plants as hyperaccumulators. Exposure to Cipro negatively impacted the heterocyst/vegetative cell ratio, nitrogenase activity, total nitrogen, and disrupted photosynthesis. This suggests that Cipro is toxic not only to the nitrogen-fixing cyanobacteria in Azolla but also to Azolla itself, demonstrating its potential use in phytoremediation of Cipro contamination
2018 - Multiple identification of most important waterborne protozoa in surfa...WALEBUBLÉ
This study evaluated a next-generation sequencing (NGS)-based method to simultaneously identify multiple important waterborne protozoan parasites (WPPs) using 18S rRNA gene amplicon sequencing. The method used a set of primers targeting the V4 region of WPPs such as Cryptosporidium, Giardia, Blastocystis, Entamoeba, Toxoplasma, and free-living amoebae. The primers were tested on a mock community and surface water sample spiked with WPP DNA. The optimized method detected Giardia intestinalis, Acanthamoeba castellanii, Toxoplasma gondii, Entamoeba histoly
This document describes the construction of a physical and genetic map of the Gluconacetobacter diazotrophicus PAL5 chromosome using pulsed-field gel electrophoresis and DNA hybridization. Key findings include:
1) G. diazotrophicus has a circular chromosome approximately 4,240 kb in size containing 4 rRNA operons.
2) Hybridization results allowed positioning of 42 genetic markers on the chromosome, including 39 single-copy genes and 3 repeated elements.
3) One rRNA operon was found to have an inverted orientation compared to the others.
This study evaluated the response of six tomato genotypes to salt stress through callus induction in vitro. Hypocotyl and cotyledon explants from seeds of genotypes G1-G6 were cultured on callus induction medium supplemented with different NaCl concentrations (0-200mM). Callus size and weight were measured after 10 and 17 days. Genotype G6 exhibited better growth at high salt concentrations (100-200mM) compared to other genotypes, indicating expression of salt tolerance genes. Genotypes G1 and G5 were most sensitive to salt as their callus size and weight decreased significantly with increasing salinity. The results demonstrate the potential to select salt tolerant cell lines for future breeding through in vitro screening of
Assimilation Of Phosphite By A. Stolonifera L. And Its In Vitro Effect On Mic...JJDempsey
Abstract on the research into the assimilation and accumulation of phosphite in A. stolonifera and the in vitro effect phosphite has on the mycelial growth of M. nivale
Environmental microbiology is the study of microbial processes in the environment, microbial communities and microbial interactions. This includes:
Structure and activities of microbial communities
Microbial interactions and interactions with macroorganisms
Population biology of microorganisms
Microbes and surfaces (adhesion and biofilm formation)
Microbial community genetics and evolutionary processes
(Global) element cycles and biogeochemical processes
Microbial life in extreme and unusual little-explored environments
A bacteriophage was isolated from soil samples collected in Puerto Rico and named Figaro. Soil samples were enriched with Bacillus cereus and Mycobacterium smegmatis bacteria to allow any bacteriophages present to replicate. A "plaque" or clear spot indicating bacteriophage growth was detected on an agar plate streaked with one enriched soil sample. The isolated bacteriophage was purified through three rounds of plating and named Figaro. Characterization of Figaro's capsid proteins was conducted through staining. The successful isolation of a bacteriophage from Puerto Rican soil supports the hypothesis that bacteriophages can be found in tropical environments. Further characterization of Figaro's genome is warranted.
This document discusses a study that characterized Salmonella enterica serotype Enteritidis (S. enteritidis) isolates from poultry farm environments in Tunisia. Samples from 8 farms yielded 21 Salmonella isolates, including 16 S. enteritidis. The S. enteritidis isolates were characterized using pulsed-field gel electrophoresis (PFGE), plasmid profiling, and antibiotic susceptibility testing. PFGE identified 2 types, plasmid profiling found 4 types, and most isolates were susceptible to antibiotics. Combined methods showed the spread of a particular S. enteritidis clone related to a major worldwide clone.
Evaluation of mancozeb activity towards various plant fungal pathogen agents ...UPL
Tests were carried out in petri dishes and concern the evaluation of conidial germination of different pathogen populations of Alternaria spp., V. inaequalis and S. vesicarium (for these last two fungi were analyzed two type of strains: sensitive e resistant to strobilurins according to our previous results (Collina et al, 2007 and Fiaccadori et al, 2011). Mancozeb was used as formulated product (solo) dissolved in sterile distillate water.
Este documento describe las características del verano en Nueva Inglaterra, incluyendo el paisaje, el clima cálido, las celebraciones del 4 de julio y el Día del Trabajo, parques temáticos populares como Six Flags y Canobie Lake Park, atracciones en Boston como los Duck Tours, Fenway Park y el Museo de Ciencias, y la ropa apropiada para el verano.
El documento resume los eventos y logros del Liceo Industrial y de Minas Ignacio Domeyko entre 2007 y 2010, incluyendo ferias científicas, competencias robóticas, reformas educativas, convenios con empresas e instituciones, y la introducción de nuevas especialidades.
2017 - Comparison of nitrifying microbial communities of two full-scale membr...WALEBUBLÉ
Barbarroja, P., Moreno-Mesonero, L., Zornoza, A., Fernández-Navarro, J., Alonso, J.L., Muñagorri, F., García, C., Álvarez, C. (2017) Comparison of nitrifying microbial communities of two full-scale membrane bioreactors treating wastewaters from municipal solid wastes using 16S rDNA gene amplicon sequencing. 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
2009,planta, nitric oxide ros chitosan, srivastava et alNupur Srivastava
1) The study found that nitric oxide (NO) production occurs downstream of reactive oxygen species (ROS) in guard cells during chitosan-induced stomatal closure in pea plants.
2) Using fluorescent probes, the study showed that chitosan exposure led to increased ROS levels within 5 minutes, while NO levels rose after 10 minutes. Inhibiting ROS prevented the rise in both ROS and NO, but inhibiting NO only prevented the rise in NO.
3) The results suggest that chitosan signaling leads to ROS production, which then triggers NO production, and both ROS and NO are required for chitosan-induced stomatal closure.
V43n4 microbiological quality of salmon (salmo salar) sold in cities of the s...Natália Nespolo
This study analyzed the microbiological quality of 31 salmon samples sold in retail markets in São Paulo, Brazil. Results found:
- Mesophilic bacteria populations ranged from 10 to 3.9 x 106 CFU/g, with frozen samples having significantly lower levels.
- Total coliforms were found in 32.25% of samples at levels up to 1.1 x 103 MPN/g. Thermotolerant coliforms were found in 19.35% of samples up to 4.6 x 102 MPN/g.
- Aeromonas sp. was isolated in 41.95% of samples ranging from 2.0 x 102 to 8.0 x 103 CFU/g. Stap
2010 - A new species of genus Metacystis from a Wastewater Treatment PlantWALEBUBLÉ
ABSTRACT. Unusual prostomatid specimens were found in the biological reactor of a wastewater treatment plant in a health resort in Valencia, Spain. These ciliates were attached to flocs unlike other free-swimming prostomatid ciliates described to date in the mixed liquor of activated sludge plants. The morphological study of this species led to a typically different combination of characteristics: elongated
cell shape, 20–30 somatic kineties, 2 perioral kineties, and 1 circumoral kinety, 1 large vacuole protruding at the terminal end, a lorica tapered toward the aperture with a smooth neck, and 11–16 annular ridges. These characteristics place this representative as a new species of the genus Metacystis—Metacystis galiani n. sp. This species became the dominant population within the biological reactor when high values of conductivity (4,244 mS/cm) and temperature (26.8 1C) were recorded.
This document outlines a study investigating the effects of statin drugs like Simvastatin on the gut microbiome in relation to clinical obesity. The hypothesis is that certain statins will selectively inhibit bacteria in the Fimicutes phylum, which are more prevalent in obese individuals. The methods test the susceptibility of various bacterial species to different statins in vitro. Results show the statins had varying effects, with some inhibiting Clostridium species. Future experiments will examine changes in fecal microbiome DNA from patients taking Simvastatin.
Cellular damage to phytophthora infestans in tomato leaves treated with oxadi...dinomasch
The document describes an ultrastructural study of cells of Phytophthora infestans, the pathogen that causes late blight in tomatoes and potatoes, after treatment with the fungicide oxadixyl. The study found that in some hyphae and haustoria of P. infestans in infected tomato leaves treated with oxadixyl, there was massive accumulation of electron-dense deposits near the plasma membrane and mitochondria. This led to mitochondrial degeneration and likely cell death. However, no changes were observed in the endoplasmic reticulum or number of ribosomes. Some hyphal cell walls were also thickened after oxadixyl treatment. The damage to fungal cells from oxadix
The document summarizes a study conducted by a biology student that found antibiotic-resistant bacteria present in higher numbers in February compared to October at a local daycare center in North Carolina. Swabs were taken from various surfaces in a toddler room in the fall and winter. Five bacterial species showing resistance to multiple antibiotics were identified in October, compared to fourteen species in February. The student used microscopy, biochemical testing, and DNA sequencing to identify the bacteria isolated. The results indicate antibiotic-resistant bacteria pose a legitimate health threat in daycare centers.
1.paola 5.anthony hernandez phages report pdf correctedpcaballero21
This study isolated and characterized a novel mycobacteriophage (bacterial virus) named Incognito from soil in Puerto Rico. Incognito was isolated from soil collected under a plantain tree and purified through plaque purification. Electron microscopy and SDS-PAGE gel electrophoresis were used to characterize the phage's morphology and protein profile. Incognito produced turbid plaques and had a concentration of 3 x 107 plaque forming units per mL. Its protein profile showed similarities to other mycobacteriophages. This study demonstrates that Puerto Rico's tropical environment supports diverse phage populations with potential applications in phage therapy.
The document summarizes research into what makes certain natural clays antibacterial. Key points:
- Antibacterial clays contain nano-scale illite-smectite and reduced iron phases that buffer water pH and oxidation state, promoting Fe2+ solubility.
- E. coli exposed to an antibacterial clay's leachate accumulate high intracellular concentrations of Fe and P, supporting a role for polyphosphate or phospholipids in regulating Fe2+.
- Excess Fe2+ overwhelms cell membrane regulatory proteins, then oxidizes inside cells to Fe3+, producing lethal hydroxyl radicals through the Fenton reaction.
- High-resolution SEM images show the nano-scale crystals and Fe
This study characterized esterases associated with profenofos resistance in the tobacco budworm, Heliothis virescens. The researchers found that esterase (EST) activities were significantly higher in profenofos-resistant larvae compared to susceptible larvae, and EST activities highly correlated with resistance to profenofos. Electrophoretic gels revealed both quantitative and qualitative differences in EST bands between resistant and susceptible larvae. A band designated A' was expressed only in resistant larvae and increased in frequency after selection with profenofos. Reduction in acetylcholinesterase sensitivity to inhibition was also observed in resistant larvae. These results suggest EST assays can detect and monitor profenofos resistance in H. v
Pesticidas em processos fermentativos do vinhoLaura Mascarin
The presence of six fungicides (azoxystrobin, cyprodinil, fludioxonil, mepanipyrim, pyrimethanil, and tetraconazole) did not negatively affect alcoholic fermentation by two yeasts (Saccharomyces cerevisiae and Kloeckera apiculata), and in some cases stimulated higher alcohol production. During malolactic fermentation by two lactic acid bacteria (Leuconostoc oenos and Lactobacillus plantarum), malic acid degradation was slightly lower in the presence of pesticides, except for mepanipyrim which had little effect. The fermentative microorganisms did not degrade or remove the
Response of aquatic fern(Azolla), to watercontaminationKavitha Cingam
The document summarizes a study on the response of the aquatic fern Azolla to contamination by the antibiotic ciprofloxacin (Cipro). The study investigated Azolla's ability to uptake Cipro and the effects on its nitrogen-fixing process. The results showed that Azolla is able to uptake concentrations of Cipro above levels that classify plants as hyperaccumulators. Exposure to Cipro negatively impacted the heterocyst/vegetative cell ratio, nitrogenase activity, total nitrogen, and disrupted photosynthesis. This suggests that Cipro is toxic not only to the nitrogen-fixing cyanobacteria in Azolla but also to Azolla itself, demonstrating its potential use in phytoremediation of Cipro contamination
2018 - Multiple identification of most important waterborne protozoa in surfa...WALEBUBLÉ
This study evaluated a next-generation sequencing (NGS)-based method to simultaneously identify multiple important waterborne protozoan parasites (WPPs) using 18S rRNA gene amplicon sequencing. The method used a set of primers targeting the V4 region of WPPs such as Cryptosporidium, Giardia, Blastocystis, Entamoeba, Toxoplasma, and free-living amoebae. The primers were tested on a mock community and surface water sample spiked with WPP DNA. The optimized method detected Giardia intestinalis, Acanthamoeba castellanii, Toxoplasma gondii, Entamoeba histoly
This document describes the construction of a physical and genetic map of the Gluconacetobacter diazotrophicus PAL5 chromosome using pulsed-field gel electrophoresis and DNA hybridization. Key findings include:
1) G. diazotrophicus has a circular chromosome approximately 4,240 kb in size containing 4 rRNA operons.
2) Hybridization results allowed positioning of 42 genetic markers on the chromosome, including 39 single-copy genes and 3 repeated elements.
3) One rRNA operon was found to have an inverted orientation compared to the others.
This study evaluated the response of six tomato genotypes to salt stress through callus induction in vitro. Hypocotyl and cotyledon explants from seeds of genotypes G1-G6 were cultured on callus induction medium supplemented with different NaCl concentrations (0-200mM). Callus size and weight were measured after 10 and 17 days. Genotype G6 exhibited better growth at high salt concentrations (100-200mM) compared to other genotypes, indicating expression of salt tolerance genes. Genotypes G1 and G5 were most sensitive to salt as their callus size and weight decreased significantly with increasing salinity. The results demonstrate the potential to select salt tolerant cell lines for future breeding through in vitro screening of
Assimilation Of Phosphite By A. Stolonifera L. And Its In Vitro Effect On Mic...JJDempsey
Abstract on the research into the assimilation and accumulation of phosphite in A. stolonifera and the in vitro effect phosphite has on the mycelial growth of M. nivale
Environmental microbiology is the study of microbial processes in the environment, microbial communities and microbial interactions. This includes:
Structure and activities of microbial communities
Microbial interactions and interactions with macroorganisms
Population biology of microorganisms
Microbes and surfaces (adhesion and biofilm formation)
Microbial community genetics and evolutionary processes
(Global) element cycles and biogeochemical processes
Microbial life in extreme and unusual little-explored environments
A bacteriophage was isolated from soil samples collected in Puerto Rico and named Figaro. Soil samples were enriched with Bacillus cereus and Mycobacterium smegmatis bacteria to allow any bacteriophages present to replicate. A "plaque" or clear spot indicating bacteriophage growth was detected on an agar plate streaked with one enriched soil sample. The isolated bacteriophage was purified through three rounds of plating and named Figaro. Characterization of Figaro's capsid proteins was conducted through staining. The successful isolation of a bacteriophage from Puerto Rican soil supports the hypothesis that bacteriophages can be found in tropical environments. Further characterization of Figaro's genome is warranted.
This document discusses a study that characterized Salmonella enterica serotype Enteritidis (S. enteritidis) isolates from poultry farm environments in Tunisia. Samples from 8 farms yielded 21 Salmonella isolates, including 16 S. enteritidis. The S. enteritidis isolates were characterized using pulsed-field gel electrophoresis (PFGE), plasmid profiling, and antibiotic susceptibility testing. PFGE identified 2 types, plasmid profiling found 4 types, and most isolates were susceptible to antibiotics. Combined methods showed the spread of a particular S. enteritidis clone related to a major worldwide clone.
Evaluation of mancozeb activity towards various plant fungal pathogen agents ...UPL
Tests were carried out in petri dishes and concern the evaluation of conidial germination of different pathogen populations of Alternaria spp., V. inaequalis and S. vesicarium (for these last two fungi were analyzed two type of strains: sensitive e resistant to strobilurins according to our previous results (Collina et al, 2007 and Fiaccadori et al, 2011). Mancozeb was used as formulated product (solo) dissolved in sterile distillate water.
Este documento describe las características del verano en Nueva Inglaterra, incluyendo el paisaje, el clima cálido, las celebraciones del 4 de julio y el Día del Trabajo, parques temáticos populares como Six Flags y Canobie Lake Park, atracciones en Boston como los Duck Tours, Fenway Park y el Museo de Ciencias, y la ropa apropiada para el verano.
El documento resume los eventos y logros del Liceo Industrial y de Minas Ignacio Domeyko entre 2007 y 2010, incluyendo ferias científicas, competencias robóticas, reformas educativas, convenios con empresas e instituciones, y la introducción de nuevas especialidades.
Este documento proporciona información sobre blogs, incluyendo su definición, herramientas para su creación y mantenimiento, y cómo las empresas y centros de información los usan. Explica que un blog es un sitio web donde los autores publican artículos de forma cronológica y permite comentarios de lectores. Las herramientas más populares son gratuitas y fáciles de usar, como Blogger y WordPress. Las empresas usan blogs para interactuar con clientes y obtener retroalimentación, mientras que los centros de información los usan para dif
Ponencia sobre la "Experiencia práctica de una empresa andaluza en EE.UU" realizada por Andrés Moreno (director de Marketing de AT4 Wireless) en el Enclave Internacional Extenda 2010
El documento describe los materiales aislantes utilizados en las máquinas eléctricas. Explica que los aislantes tienen muy baja conductividad eléctrica y se usan para asegurar el aislamiento entre conductores y piezas metálicas. Luego describe varias propiedades importantes de los aislantes como la resistencia de aislamiento, rigidez dieléctrica y constante dieléctrica. Finalmente, explica que es necesario eliminar el aire en los aislantes mediante impregnación para mejorar su aislamiento.
The document discusses the potential for distance learning through the use of e-infrastructures. It notes that the amount of data and knowledge is growing exponentially but education is not keeping pace. E-infrastructures like DEISA provide distributed computing resources across Europe that could enable new forms of distance learning beyond the classroom through interactive simulations, collaborations, and access to scientific tools. The prototype e-School project aims to bridge education and science by providing a virtual laboratory and digital repository accessible through portable devices to support inquiry-based learning.
The document contains English, Uighur, and Chinese phrases for common greetings, expressions, and short conversations. There are over 170 phrases included that cover topics such as introductions, agreements/disagreements, requests, well-wishes, and comments on activities or events. The phrases are presented with the English first, followed by the Uighur translation, and then the Chinese translation.
Income Access provides customisable affiliate marketing software solutions for the iGaming industry. Their software allows operators to consolidate multiple brands, products, territories, and programs onto a single platform. It offers tracking and reporting features, integrations with major providers, and tools for communication and administration. Benefits for affiliates include access to statistics, continuous commissions, and industry-leading innovation.
El Padre Juan le pidió a la Hermana Magdalena que lo ayudara a bañarse. Mientras lo lavaba, él guió su mano hacia sus genitales y le dijo que ahí guardaba la llave del cielo. Luego abusó sexualmente de ella, diciéndole que esto la salvaría. La Hermana Magdalena le contó emocionada lo sucedido a la Sor María, quien reconoció que el Padre Juan era un depredador sexual.
Este documento es una factura de telecomunicaciones emitida a nombre de Alejandro en Cádiz. Incluye los cargos por las cuotas de mantenimiento de la línea fija y móvil por un total de 4,87 euros, las cuotas de llamadas de servicio fijo por un total de 57 euros, y los cargos por el uso de datos móviles por un total de 9,51 euros. El importe total de la factura asciende a 71,38 euros más IVA.
The document proposes establishing a pilot program and intergovernmental authority for coordinating connected and autonomous vehicle (C-ITS) infrastructure deployment in Michigan. It summarizes that Michigan leads in automotive research but must continue leading in both technology creation and the environment that supports innovation. It outlines the need for coordinated infrastructure to support emerging technologies like vehicle-to-vehicle communication. The proposed authority would include Oakland County, MDOT, RCOC and automotive companies to guide C-ITS infrastructure deployment through a pilot program along I-75 in Oakland County.
Un tattoo es una imagen que permanece en el cuerpo para siempre y tiene un significado personal para la persona. Existen varios tipos de tatuajes como tribales, góticos, orientales y de puntos. Es importante mantener un tatuaje limpio e hidratado y evitar el agua durante las primeras horas para que cicatrice adecuadamente.
This document introduces eigenvalues and eigenvectors through examples. It begins by discussing how linear algebra can be used to solve systems of equations, moving from the "row picture" of intersecting lines to the "column picture" of linear combinations of vectors. It introduces key concepts like vectors, scalars, and linear combinations. The document then discusses how to represent systems of equations using matrices by choosing appropriate reference vectors. This allows solving problems algebraically by finding linear combinations of vectors rather than geometrically finding intersections of lines.
El documento proporciona información sobre la hepatitis B y el VIH/SIDA. Resume el proceso de la enfermedad de la hepatitis B, incluidos los conceptos, agentes causales, epidemiología, efectos, marcadores de riesgo y manifestaciones. También describe las medidas de prevención y control de la hepatitis B y el VIH/SIDA, así como los recursos comunitarios relacionados. Finalmente, analiza las precauciones que deben tomarse durante los viajes internacionales según el Reglamento Sanitario Internacional.
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1) O documento discute os efeitos da presença de bolhas de ar no escoamento de água em medidores de vazão do tipo turbina.
2) Foi realizado um estudo experimental para avaliar divergências nas leituras dos medidores em condições bifásicas e monofásicas.
3) Os resultados podem contribuir para o debate sobre a influência de bolhas de ar nos valores cobrados nas contas de água.
This document analyzes the aerodynamic stability and trajectory simulation of a student sounding rocket called the SMART rocket. It develops methods to evaluate the rocket's stability when disturbed during flight and models potential perturbations from thrust errors, airframe imperfections, and wind. An iterative design process improves the rocket's stability margins. Flight trajectory is studied using simulation software under various launch conditions and with/without disturbances. The results provide critical design insights and define allowable launch parameters to fulfill mission requirements of safe recovery and achieving minimum altitude and speed.
Adrian Weisberg Ajax World Presentationrajivmordani
This document discusses using real-time internet applications (RIAs) for network health monitoring. It addresses two main challenges: 1) that HTTP was not designed for real-time communication, which can be solved using Comet techniques, and 2) not wanting to translate all monitoring protocols into HTTP, which can be solved using TCPSocket communication through Comet. It provides an example of using these techniques to build a browser-based GUI for monitoring an email marketing platform with distributed, elastic resources running on EC2.
2017 - Environmental Ordination of Filamentous Bacteria in Activated SludgeWALEBUBLÉ
Reference:
Zornoza, A., Serrano, S. and Alonso, J.L. (2017) Environmental Ordination of Filamentous Bacteria in Activated Sludge. In: Abstracts of the 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
2004 paper primer ctx dissemination of ctx m-type -lactamases among clinical ...JairAlexanderTllez
This document summarizes a study analyzing 19 clinical isolates of Enterobacteriaceae (16 E. coli and 3 K. pneumoniae) collected from four hospitals in Paris, France between 2000-2002 that exhibited resistance to extended-spectrum cephalosporins. Testing found the resistance was due to various CTX-M-type beta-lactamase enzymes, predominantly CTX-M-15. Most isolates produced both TEM-1 and CTX-M enzymes. The blaCTX-M genes were located on large plasmids and often upstream of the insertion sequence ISEcp1. Five isolates had identical plasmid fingerprints, suggesting clonal dissemination of CTX-M-15-producing strains in
This study investigated the role of the transcriptional regulator ToxR in biofilm formation and motility in the pathogenic bacterium Vibrio parahaemolyticus. The study found that:
1) A V. parahaemolyticus mutant lacking ToxR showed decreased biofilm formation and reduced swarming and swimming motility compared to the wild type strain.
2) The ToxR regulator was required for rugose colony formation and expression of genes involved in exopolysaccharide production, which are important for biofilm formation.
3) ToxR did not regulate switching between opaque and translucent colony types in V. parahaemolyticus, which is associated with capsular polysaccharide production.
Improvement in nutritional quality of spices through potential use of titan...ShreyaMandal4
Nutrient deficiency in food crops is seriously affecting human health, especially those in the rural areas. There are several ways of fortifying the nutrients in food such as dietary diversification, use of drugs and industrial fortification. One of the most intensively consumed metal oxide nanoparticles (NPs) worldwide, titanium dioxide nanoparticles (nTiO₂) is applied in many widely used products, such as in food production, in personal care products, in electronics and pharmaceuticals, and in environmental remediation. To date, little information is available on whether nTiO₂ amendment can enhance vegetable nutritional quality and alter spatial distribution of the important nutrient elements in the edible tissues. To address this knowledge gap, the vegetable coriander was selected as a model plant species. Coriander is an aromatic annual herb in Apiaceae family and possesses significant nutritional and medicinal properties. In this study, coriander (Coriandrum sativum L.) was treated with 0, 50, 100, 200, and 400 mg/L nTiO₂ to evaluate their possible benefit to plant growth and nutritional quality under hydroponic conditions. Observations showed that 50 mg/L nTiO₂ significantly increased the root and shoot fresh biomass by 13.2 % and 4.1 %, respectively, relative to the control. nTiO₂ at this level promoted shoot K, Ca, Mg, Fe, Mn, Zn, and B accumulation, while spatial distribution of K, Ca, Fe, Mn, Cu and Zn in coriander leaves was not affected. No nTiO₂ internalization or translocation to shoots occurred. 400 mg/L nTiO₂ significantly reduced root fresh biomass by 15.8 % and water content by 6.7%. Moreover, this high dose induced root cell membrane wrinkling, attributable to their aggregation and adsorption on root surfaces. At 100–400 mg/L concentration, antioxidant defense systems (SOD, CAT and APX) in plant were triggered to alleviate oxidative stress. At an appropriate dose (50 mg/L), nTiO₂ can improve nutrient quality of edible tissues without exerting toxicity to plant or posing health risk to consumers.
Effects of organic and mineral fertilizers on total antioxidant, polyphenolic...Alexander Decker
This study examined the effects of organic and mineral fertilizers on antioxidant, polyphenol, and carotenoid content in orange-fleshed sweet potato tubers. Organic fertilizer significantly increased all three phytochemicals, with annual applications having the highest levels. Mineral fertilizers also significantly affected levels, with combinations of nitrogen, phosphorus, and potassium influencing different compounds. The combination of minimal mineral doses with annual or biennial organic fertilizer produced the highest antioxidant, polyphenol, and carotenoid concentrations in the tubers.
poster presented at the Internation Workshop on Edible Mycorrhizal Mushroom in Morocco 2014.
We describe the results on the ecology of Tuber borchii in natural sites and truffle plantation in worth Portugal, including molecular diversity and borchii mating types.
Antimicrobial susceptibility of isolated E.coli from different water sources ...Sulieman Bahar
This study tested the antimicrobial susceptibility of E.coli isolated from different water sources in Nyala Town, Sudan. E.coli was isolated from 50 water samples using standard methods and tested against 12 commonly used antimicrobial agents. The results showed that the E.coli strains were most sensitive to Ciprofloxacin, Co-Trimoxazole and Chloramphenicol and most resistant to Tetracycline and Ampicillin/Sulbactam. This indicates multiple antibiotic resistant E.coli exist in the water sources of the study area, making the water potentially unsafe for drinking.
This document summarizes the discovery of a new class of insecticides called anthranilic diamides. The compounds were found to activate ryanodine receptors in insects, causing uncontrolled release of calcium stores and ultimately death. The document describes the synthesis of various analogs of anthranilic diamides containing modifications like halogen substitutions, ring sizes, and heterocyclic moieties. Key intermediates and reaction schemes are provided to illustrate the various routes taken to synthesize the analogs. Testing showed certain analogs had exceptional insecticidal activity, with one example called DP-23 being particularly potent.
The document summarizes a study that investigated the toxicity of five compounds - pentachlorophenol, trimethyltin, dibutyltin, nickel, and cyanide - on brine shrimp (Artemia franciscana). Trimethyltin proved the most toxic, while nickel was the least. Cyanides showed moderate toxicity, and pentachlorophenol toxicity was similar to dibutyltin. Tests found synergistic interactive effects between pentachlorophenol and each organotin, while the organotins showed antagonistic interaction. Finally, exposure to all three compounds together produced synergistic mortality.
This study reanalyzed data from 1990 to 2010 on the effects of highly diluted thyroxine on amphibian metamorphosis. Researchers tested thyroxine at a 30x dilution on highland frogs (Rana temporaria) in 20 sub-experiments over the two decades. They developed a method to normalize results across experiments of varying durations. The results showed thyroxine at a 30x dilution slowed metamorphosis, consistent with previous studies using other pooling methods. When pretreated with molecular thyroxine, frogs also showed slowed metamorphosis after treatment with the 30x dilution in most of 10 sub-experiments.
2017 - Environmental ordination of nitrifying bacterial community dynamics in...WALEBUBLÉ
Biological nitrification-denitrification is commonly used for nitrogen removal in Wastewater Treatment Plants (WWTPs). Nitrification, is the sequential oxidation of ammonia via nitrite to nitrate. This process is catalysed by ammonia-oxidizing bacteria and archaea (AOB and AOA) and nitrite-oxidizing bacteria (NOB), whose cooperation is needed to achieve complete nitrification. They are a phylogenetically diverse guild with pronounced ecological niche specialization and they differ from each other in fundamental physiological and molecular traits. Although the nitrification process in WWTPs has been investigated in depth, the response of microbial
communities are still a focus of considerable interest due to their high sensitivity to inhibitory compounds and environmental factors, that results in repeated breakdowns of nitrification performance. Most of studies have been mainly descriptive and/or exploratory and environmental interpretation has not been addressed. In this study, we focus on the environmental ordination of the relationships between biological variables (nitrifying bacterial community) and physicochemical variables (nitrogen compounds and environmental conditions), to propose new strategies to improve the performance of the nitrogen removal process in WWTPs.
This study evaluated different sanitation methods on the quality of fresh cherry and plum salad over 10 days of storage at 4°C. The fruit was minimally processed and packaged under modified atmosphere. Using UV-C radiation and 10g/L ascorbic acid (Treatment 2) was most effective in reducing microbial growth, maintaining antioxidant compounds and sensory quality compared to water (Control) or ascorbic acid alone. Treatment 2 reduced mesophilic bacteria by 1.15 log CFU/g and psychrotrophic bacteria by 1 log CFU/g versus the Control after 10 days. It also better preserved color, phenolic content, anthocyanins and antioxidant activity over time. This combinated treatment shows potential as an environment
Influence of carbon and nitrogen sources on the spore yield of Trichoderma ha...Open Access Research Paper
The influence of carbon and nitrogen sources on the spore yield of T. harzianum in fed-batch culture was investigated. T. harzianum was cultivated in liquid culture media under the effect of different carbon and nitrogen sources at different concentration using a fed-batch process. It was observed that among the various carbon sources studied, glucose (2.5 g l-1) gave the highest spore yield of 2.81±0.14 while starch (15.0 g l-1) gave the lowest spore yield (0.22±0.17). The effect of nitrogen sources revealed that 1.0 g l-1 of casein enhanced the highest spore yield (2.88±0.02) while the lowest spore yield (0.23±0.02) was recorded in medium containing soy meal (9.0 g l-1) preparation. The results revealed that T. harzianum has the ability to utilize various carbon and nitrogen compounds and produce high spore yield at low carbon and nitrogen concentration. This can be considered during industrial production of T. harzianum spores for biocontrol.
This article examines the effectiveness of two species of entomopathogenic nematodes (Heterorhabditis bacteriophora HP88 and Heterorhabditis indica LPP1) at controlling partially engorged females of the tropical horse tick (Dermacentor nitens). In laboratory experiments, the nematodes were applied at different concentrations to the ticks. Both nematode species significantly reduced egg mass weight, egg production, and hatching rates at higher concentrations. H. bacteriophora HP88 and H. indica LPP1 achieved over 95% control of D. nitens females at the highest concentration tested, demonstrating their potential as biological control agents for this tick species.
Antibiotic resistance and molecular characterizationAlexander Decker
This document reports on a study of antibiotic resistance and molecular characterization of Salmonella isolated from stool samples of diarrhoeal patients in Ile-Ife, Nigeria. A total of 69 Salmonella isolates (S. Typhimurium, S. Typhi, S. Paratyphi A) were obtained from 187 stool samples. The isolates showed high resistance to nitrofurantoin, ceftriazone, and augmentin. Sixty-seven isolates were resistant to at least two antibiotic classes, with resistance to combinations of four antibiotics being most common. Plasmid DNA analysis of resistant isolates identified multiple plasmids ranging from 1.46-23.13 kbp. Resistance genes blaCTX and
Antibiotic resistance and molecular characterizationAlexander Decker
This document reports on a study of antibiotic resistance and molecular characterization of Salmonella isolated from stool samples of diarrhoeal patients in Ile-Ife, Nigeria. A total of 69 Salmonella isolates (82.6% S. Typhimurium, 10.1% S. Typhi, 7.3% S. Paratyphi A) were obtained from 187 stool samples. The isolates showed high resistance to nitrofurantoin (100%), ceftriazone (97.2%), and gentamicin (94.2%). Sixty-seven isolates (97.1%) were resistant to at least two antibiotic classes, with 32 resistance patterns identified. Multiple plasmids ranging from 1.
The International Journal of Engineering & Science is aimed at providing a platform for researchers, engineers, scientists, or educators to publish their original research results, to exchange new ideas, to disseminate information in innovative designs, engineering experiences and technological skills. It is also the Journal's objective to promote engineering and technology education. All papers submitted to the Journal will be blind peer-reviewed. Only original articles will be published.
Colletotrichum causes anthracnose in crops around the world producing postharvest losses up to 60%. There are a great variety of Colletotrichum strains isolated from mango orchards. Thus, it is important to characterize their pathogenicity, as well as to perform a correct identification, in order to implement good strategies to eradicate the produced disease. The aim of this work is to identify Colletotrichum spp. and to determine the production of Pectate Lyase (PL) as a virulence factor in the pathogenicity process. Macroscopic characteristics of isolated colony vary from grey to salmon, sometimes showing luxuriant orange conidial masses with grey or white bottom. Conidia vary from 10.39 to 14.83 × 2.75 to 3.40 μm corresponding to C. gloeosporioides or C. acutatum according to Sutton. Growth rates vary from 0.1948 to 0.2239 day-1. The pectate lyase activity was induced by mango cells (240.81 VS 398U/L). According to CgInt and ITS4 PCR amplification M2V and SA correspond to C. gloeosporioides.
EWMA Conference-Ep442-MODELLING WOUND BIOFILMS IN A THERMO-REVERSIBLE MATRIX ...EWMA
Benjamin Taylor1, 2, David Williams1 & Jon Nosworthy3.
1Cardiff University School of Dentistry
2Knowledge Transfer Partnership Associate
3Advanced Medical Solutions plc
EWMA Conference-Ep442-MODELLING WOUND BIOFILMS IN A THERMO-REVERSIBLE MATRIX ...EWMAConference
MODELLING WOUND BIOFILMS IN A THERMO-REVERSIBLE MATRIX WITH FLORESCENT MARKERS
Benjamin Taylor1, David Williams2, Jon Nosworthy3
1Cardiff University/Advanced Medical Solutions (Cardiff/Winsford, United Kingdom);
2Cardiff University (Cardiff, United Kingdom);
3Advanced Medical Solutions (Winsford, United Kingdom).
Similar to 2013 - Identification and abundance of thiothrix in wwt ps treating domestic wastewater using fish (20)
Este documento es un certificado de aprobación que otorga al Sr. José Airam el título de Técnico en Bioindicación y Control del Proceso Biológico de Estaciones Depuradoras de Aguas Residuales. El Sr. Airam completó con éxito 114 horas de estudios en este plan de dos años académicos que le faculta para monitorear y optimizar estas instalaciones. El certificado fue expedido por el Dr. Andrés Miguel Zornoza Zornoza, director de la plataforma educativa Walebublé del área H2O de H
2015 - Archaeal populations in full-scale autotrophic nitrogen removal biorea...WALEBUBLÉ
This study analyzed the archaeal populations present in three full-scale autotrophic nitrogen removal bioreactors located in the Netherlands with different technologies: partial nitritation/anammox, CANON, and DEMON. Quantitative PCR and 16S rRNA gene pyrosequencing revealed the presence of archaea in all three bioreactors, comprising over 0.3% of the total prokaryotic communities. Phylogenetic analysis identified archaeal species related to methanogenesis and nitrogen cycling. The results confirm archaea play a role in full-scale anammox processes, though their functions require further study. An inverse correlation was also found between archaeal abundance and bioreactor performance.
2018 - CFD simulation of fluid dynamic and biokinetic processes within activa...WALEBUBLÉ
This document describes a CFD simulation of fluid dynamics and biokinetic processes within an activated sludge reactor (ASR) operating under intermittent aeration. The CFD model considers fluid dynamics, oxygen transfer, and biological processes described by Activated Sludge Model No. 1 (ASM1). The model is used to evaluate two different aeration system configurations for an ASR in terms of their ability to satisfy effluent requirements with minimum energy consumption. Results show that modifying the air diffuser layout can improve energy consumption by 2.8%, and reducing the air flow rate per diffuser improves energy consumption by 14.5%. The model provides insight into aeration inefficiencies within ASRs.
2016 - EXPERIENCIA PILOTO DE SIMULACIÓN PARA LA MEJORA DEL RENDIMIENTO DE ELI...WALEBUBLÉ
El documento describe un estudio de modelado y simulación de una estación depuradora de aguas residuales (EDAR) que tenía problemas persistentes para cumplir con los límites de nitrógeno total en el efluente. Se generó un modelo matemático integral de la EDAR y se calibro con datos experimentales. Las simulaciones permitieron determinar estrategias operativas óptimas para lograr una concentración de nitrógeno total inferior a 10 mg/L con costos mínimos.
2018 - APLICACIÓN DE HERRAMIENTAS DE SIMULACIÓN PARA CONTROL Y SUPERVISIÓN EN...WALEBUBLÉ
El documento describe un estudio de modelado y simulación de la estación depuradora de aguas residuales de El Chaparral. Se generó un modelo matemático que fue calibrado y validado con datos experimentales. El modelo se utilizó para simular diferentes escenarios operativos y la ampliación planificada de la planta, lo que ayudó a optimizar la operación y predecir el rendimiento futuro.
2017 - Analysis of nitrifying microbial communities by FISH and 16S rRNA ampl...WALEBUBLÉ
Nitrification, the sequential oxidation of ammonia via nitrite to nitrate, is an important process for nitrogen removal from municipal wastewater. This process is catalysed by ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB), two different groups of slow-growing microorganisms whose cooperation is needed to achieve complete nitrification. High efficiency and stability of this process is required for wastewater treatment plants (WWTPs) operational optimization due to
nitrification is often subjected to recurring collapse in many WWTPs. Therefore, a better understanding of the microbial ecology of nitrifying bacteria in WWTPs could
potentially improve the nitrification stability. Novel high-throughput molecular methods, as next generation sequencing (NGS), are nowadays providing detailed knowledge on the microorganisms governing wastewater treatment systems. This
methods in conjunction with the environmental ordination of the relationships between biological variables (nitrifying bacterial community) and physicochemical variables (nitrogen compounds and environmental conditions) provide a powerful
tool to elucidate how selection pressures imposed by operational and environmental conditions affect community diversity and dynamics within activated sludge systems.
2017 - Guía de iniciación WEST (Español): Modelado y Simulación en EDARWALEBUBLÉ
Este documento presenta una guía de uso de un software de simulación de plantas de tratamiento de aguas residuales. Explica los diferentes niveles de simulación, introduce el modelo matemático ASM1 y describe las utilidades y funcionalidades del software como la introducción de datos de entrada, librería de bloques, ventanas de opciones y funciones disponibles en las pestañas.
2017 - (IAGUA Magazine 16) El software libre de simulación llega a la EDARWALEBUBLÉ
Este documento describe el desarrollo de software libre para simular procesos y dinámica de fluidos computacional (CFD) en plantas de tratamiento de aguas residuales. Se menciona el software Lynx CFD y Lynx ASM1, desarrollados por el Aula de Bioindicación Gonzalo Cuesta y Lynx Simulations para simular reactores biológicos, canales UV y el proceso completo de una planta, respectivamente. El objetivo es proveer herramientas de simulación accesibles para optimizar el diseño y operación de plantas de trat
2013 - Estudio de las relaciones de las bacterias filamentosas no ramificadas...WALEBUBLÉ
Tena, S. (2013) Estudio de las relaciones de las bacterias filamentosas no ramificadas (Microthrix y tipo 0581) formadoras de espumas con los parámetros Operacionales y Físico- Químicos en una EDAR de la Comunidad Valenciana. Trabajo final de Máster en Ingeniería Ambiental. Valencia: Universitat Politècnica de València.
www.abgc
2016 - Estudio de la dinámica de protistas y metazoos en un reactor biológico...WALEBUBLÉ
Este documento presenta un estudio sobre la dinámica de protistas y metazoos en un reactor biológico de aireación prolongada con macrófitas en flotación y su relación con las variables fisicoquímicas. El estudio se realizó en la EDAR de una urbanización que utiliza este sistema de tratamiento. Se tomaron muestras del agua de entrada y salida, del licor mezcla y de la biopelícula de las macrófitas en diferentes puntos del proceso para analizar las comunidades microbianas presentes y su evoluc
2014 - Estudio de las relaciones del morfotipo Nosotocoida limicola con los p...WALEBUBLÉ
Calvo, S. (2014) Estudio de las relaciones del morfotipo Nosotocoida limicola con los parámetros operacionales y fisico-químicos en EDAR de la Comunidad Valenciana. Trabajo final de Máster en Ingeniería Ambiental. Valencia: Universitat Politècnica de València.
www.abgc.es
2014 - Identificación y cuantificación del morfotipo Haliscomenobacter hydros...WALEBUBLÉ
Ferrer, M. (2014) Identificación y cuantificación del morfotipo Haliscomenobacter hydrossis formador de bulking mediante la técnica FISH y estudio de su relación con los parámetros operacionales y físico-químicos en EDAR de la Comunidad Valenciana. Trabajo final de Máster en Ingeniería Ambiental. Valencia: Universitat Politècnica de València.
www.abgc.es
2012 - Microscopía convencional versus FISH en la identificación, abundancia ...WALEBUBLÉ
Andújar, A. B. (2012) Microscopía convencional versus FISH en la identificación, abundancia y ecofisiología de los morfotipos filamentosos 0803, 0914 y 0092 en fangos activos. Trabajo final de máster en Ingeniería Ambiental. Valencia: Universitat Politècnica de València.
www.abgc.es
2017 -Study of enzymatic activities and bacterial communities in two full-sca...WALEBUBLÉ
Reference:
Fernández-Navarro, J., Moreno-Mesonero, L., Zornoza, A., Amorós, I., Alonso-Molina, J.L., Muñagorri, F., Álvarez, C. (2017) Study of enzymatic activities and bacterial communities in two full-scale MBR plants treating wastewaters from municipal solid wastes. In: Abstracts of the 7th congress of European microbiologists FEMS 2017, Valencia, Spain, 9-13 July 2017.
www.abgc.es
2013 - Estudio de las relaciones de Gordonia con parámetros operacionales y f...WALEBUBLÉ
Nuñez, J.M. (2013) Estudio de las relaciones de Gordonia con parámetros operacionales y físico-químicos en EDAR de la Comunidad Valenciana. Trabajo final de Máster. Valencia: Universitat Politècnica de València.
2017 - Plausible Bioindicators of Biological Nitrogen Removal Process in WWTPsWALEBUBLÉ
The document describes a study that aimed to identify potential bioindicators of biological nitrogen removal in wastewater treatment plants (WWTPs). Samples were collected from six WWTPs over one year and analyzed for protist and metazoan populations. Multivariate analyses revealed differences in biological communities between bioreactors and seasons. Models identified several protist and metazoan species correlated with nitrogen removal efficiency. Species were grouped based on their associations with different nitrogen compounds in plant effluent, with some correlated with good nitrification and others with poor nitrification performance.
2011 - Estudio de la población de bacterias nitrificantes y su relación con l...WALEBUBLÉ
Este documento presenta un estudio realizado sobre la población de bacterias nitrificantes y su relación con parámetros físico-químicos, biológicos y operacionales en una EDAR con sistema convencional de fangos activos. Se identificó y cuantificó la población de bacterias amonio y nitrito oxidantes a lo largo de un año usando la técnica FISH. Se encontró que las bacterias amonio oxidantes dominantes eran del género Nitrosomonas y las nitrito oxidantes del género Nitrospira
2011 - Estudio de la población de bacterias nitrificantes y su relación con l...
2013 - Identification and abundance of thiothrix in wwt ps treating domestic wastewater using fish
1. Identification and abundance of Thiothrix in wastewater treatment plants
treating domestic wastewater using fluorescence in situ hybridization
ABSTRACT
CODE
821
W56
V. Romero, A. Zornoza, and J.L. Alonso
Instituto Universitario de Ingeniería del Agua y Medio Ambiente, (IIAMA). Universitát Politècnica de València, Spain (e-mail: jalonso@ihdr.upv.es).
INTRODUCTION
Bacteria that belong to the genus Thiothrix are characterized by distinctive morphological features, such as filamentous growth and accumulation of sulphur
granule when incubated in the presence of sulphide or thiosulphate. The Thiothrix morphotypes occur in municipal and industrial WWTPs with and without
nutrient removal [1]. Traditionally, the problems of biological bulking have been monitored by classical microscopy such as morphological observation and
polychromatic staining [2]. Few works have been carried out for the detection of Thiothrix in WWTP samples with molecular procedures such as the fluorescence
in situ hybridization (FISH) technique. In this study, we investigated several Spanish WWTPs with the FISH technique to analyze the composition of Thiothix
species and their relathionships with physico-chemical and operational parameters.
MATERIAL AND METHODS
The sampling period was conducted between February to November 2012 in 3
urban WWTPs with conventional activated sludge system (CAS) and 1 urban
WWTP with extended aeration system. Fortnightly samples of mixed liquor were
analyzed by light microscopy and quantitative FISH.
In the FISH analysis a set of 8 rRNA-targeted nucleic acid probes covering
Thiothrix genus and species were used (Table 1). The abundance of filamentous
bacteria in the 139 activated sludge samples was measured according to the
subjective scoring method of Eikelboom (2000) where observations are rated on
scale from 0 (none) to 5 (extensive growth). The hybridizations were performed at
46 º C for 2 hours. All the probes (Table 1), labelled at the 5’ end with Tamra, were
purchased from TibMobiol, Germany.
%FA: Formamide percentage; G123TC y Comp. TNI y TFR: Competitor probes
RESULTS
Thiothrix filaments were present in the four WWTP analyzed. In 92 (66,2 %) of the samples, Thiothrix filaments were observed with the probe G123T. It was
evident that mixed populations of Thiothrix (figure 1) were present in the activated sludge samples investigated, the observed differences were in the relative
abundance of the various groups. These findings were supported by the results obtained using conventional microscopy. T. eikelbomii (figure 2), T.
fructosivorans (figures 3) and T. nivea (figure 4) were common (FI>3) in two of the four WWTP analyzed. No fluorescence signal was detected with the probes G1B
(T. disciformis), G3M (T. flexilis) and Meg1028+Meg 983 (Meganema perideroedes). Redundancy analysis (RDA) ordination diagram (biplot) suggested different
behaviour among species within genus Thiothrix. T. eikelbomii showed a positive relationship with the reactor temperature (Tr) (figure 5). Conversely, T.
fructosivorans was negatively related with reactor temperature (Tr). T. nivea and T. fructosivorans were associated with high sludge retention time (SRT) values
and low values of organic loading rate (OLR). T. nivea showed a positive relationship with the hydraulic retention time (HRT) and negative with the influent
carbohydrates and proteins. T. eikelbomii and T. nivea were associated with nitrogen and phosphorus deficit (BOD5/TN/TP) (figure 6).
Figure 1: Thiothrix spp. (G123T probe), A:
phase contrast, B: epifluorescence 1000X
Figure 3: T. fructosivorans (TFR probe), A: phase contrast,
B: epifluorescence, 1000X
Figure 2: T. eikelbomii (G2M probe), A: phase
contrast, B: epifluorescence 1000X
A
B
Figure 5: RDA between Thiothrix species and
physico-chemical parameters
Figure 6: RDA between
operational parameters
Figure 4: T. nivea (TNI probe), A: phase contrast, B:
epifluorescence, 1000X
REFERENCES
[1] Eikelboom D (2000) Process control of activated sludge plants by microscopic investigation. IWA Publishing, London, England.
[2] Jenkins D., Richard M., Daigger G. (2004) Manual on cases and control of activated sludge bulking and foaming. Lewis Publishers, Chelsea, Michigan.
Thiothrix species and