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“Estimation of Free CO2 in given
Water Sample”
Presented by
Manoj Joshi
Department of Zoology
UCoS,MLSU, Udaipur.
Class- B.Sc. III Year
Practical
Date: 15.10.2020
Time: 03:00-05:00PM
CO2 present in water is called free CO2. Free CO2 react with H2O and
form carbonic acid which is titrated with strong base (NaOH) or weak
base (Na2CO3).
Carbonic acid convert into nutral sodium bicarbonate, by adding
phenolphtalin indicator during titration at pH 8.3 it gives pink colour.
2NaOH + CO2=== Na2CO3 + H2O
Na2CO3 + H2O + H2O + CO2 == 2NaHCO3.
Free CO2 titratte by two methods:-
(a) NaOH (b) Na2CO3
(a) By NaOH:-
Reagents:-
i. 0.05 N NaOH:- 40 gm + 1000 ml DW Heat and then Cool.
ii. Phenolpthalien:- 50 ml 95% Ethenol + 500 mg Phenolpthalien.
Procedure:-
•Collect 250-300 ml sample in Nessler tube carefully.
•Take 100 ml sample from collected sample in a conical flask.
•Add few drops of indicator if it shows pink colour then no free CO2 present in
sample.
•If it remain colourless then titrate it up to pink colour appearance.
•Note the reading and repeat the process three times for better results.
Observation table:-
S. No
Sample
Vol. (ml)
Burette Reading
Mean
Start End Total
1 100 0.0
2 100 0.0
3 100 0.0
Calculation:-
Chloride mg/L= A x N of NaOH X 1000 x 44
ml of sample
= A x 0.05 x 1000 x 44
100
= A x 22mg/L.
1644_B.Sc. III Yr 11 (1).pptx

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1644_B.Sc. III Yr 11 (1).pptx

  • 1. “Estimation of Free CO2 in given Water Sample” Presented by Manoj Joshi Department of Zoology UCoS,MLSU, Udaipur. Class- B.Sc. III Year Practical Date: 15.10.2020 Time: 03:00-05:00PM
  • 2.
  • 3. CO2 present in water is called free CO2. Free CO2 react with H2O and form carbonic acid which is titrated with strong base (NaOH) or weak base (Na2CO3). Carbonic acid convert into nutral sodium bicarbonate, by adding phenolphtalin indicator during titration at pH 8.3 it gives pink colour. 2NaOH + CO2=== Na2CO3 + H2O Na2CO3 + H2O + H2O + CO2 == 2NaHCO3. Free CO2 titratte by two methods:- (a) NaOH (b) Na2CO3
  • 4. (a) By NaOH:- Reagents:- i. 0.05 N NaOH:- 40 gm + 1000 ml DW Heat and then Cool. ii. Phenolpthalien:- 50 ml 95% Ethenol + 500 mg Phenolpthalien. Procedure:- •Collect 250-300 ml sample in Nessler tube carefully. •Take 100 ml sample from collected sample in a conical flask. •Add few drops of indicator if it shows pink colour then no free CO2 present in sample. •If it remain colourless then titrate it up to pink colour appearance. •Note the reading and repeat the process three times for better results.
  • 5. Observation table:- S. No Sample Vol. (ml) Burette Reading Mean Start End Total 1 100 0.0 2 100 0.0 3 100 0.0 Calculation:- Chloride mg/L= A x N of NaOH X 1000 x 44 ml of sample = A x 0.05 x 1000 x 44 100 = A x 22mg/L.